Mutagenetix > Incidental Mutation

Incidental Mutation 'R8907:Gm7298'

678442

Institutional Source

Beutler Lab

Gene Symbol

Gm7298

Ensembl Gene

ENSMUSG00000108022

Gene Name

predicted gene 7298

Synonyms

MMRRC Submission

068763-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.285) question?

Stock #

R8907 (G1)

Quality Score

219.009

Status

Not validated

Chromosome

Chromosomal Location

121711454-121761598 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121741817 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 413 (V413A)

Ref Sequence

ENSEMBL: ENSMUSP00000145242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000204124]

AlphaFold

A0A0N4SVU1

Predicted Effect

probably benign
Transcript: ENSMUST00000204124
AA Change: V413A

PolyPhen 2 Score 0.104 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000145242
Gene: ENSMUSG00000108022
AA Change: V413A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:A2M_N	128	221	4e-18	PFAM
A2M_N_2	449	599	1e-45	SMART
A2M	740	830	2.1e-39	SMART
Pfam:Thiol-ester_cl	963	992	1.9e-15	PFAM
Pfam:A2M_comp	1012	1268	1.6e-90	PFAM
A2M_recep	1378	1465	4.3e-42	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5930422O12Rik	A	G	8: 33,919,398 (GRCm39)	I73V	probably damaging	Het
Agap3	T	C	5: 24,679,629 (GRCm39)	I109T	probably benign	Het
Ahnak	G	A	19: 8,986,452 (GRCm39)	V2579M	probably benign	Het
Akap13	T	C	7: 75,260,444 (GRCm39)	S220P	probably benign	Het
Akap13	C	G	7: 75,260,456 (GRCm39)	P224A	probably damaging	Het
Alpk1	G	A	3: 127,474,642 (GRCm39)	Q454*	probably null	Het
Asrgl1	C	T	19: 9,090,506 (GRCm39)	G280D	probably damaging	Het
Atp6v0d1	TTCTTACCTC	T	8: 106,251,782 (GRCm39)		probably benign	Het
Atxn2l	T	A	7: 126,099,425 (GRCm39)	D204V	probably damaging	Het
B430306N03Rik	T	C	17: 48,628,100 (GRCm39)	Y177H	probably damaging	Het
Bcl2l14	G	A	6: 134,400,585 (GRCm39)	C2Y	probably damaging	Het
Bltp1	T	A	3: 37,002,295 (GRCm39)	Y1413*	probably null	Het
Bphl	A	T	13: 34,230,930 (GRCm39)	M125L	possibly damaging	Het
Cdh10	A	T	15: 19,013,521 (GRCm39)	N707I	probably damaging	Het
Ceacam1	T	A	7: 25,171,444 (GRCm39)	D340V	possibly damaging	Het
Cklf	G	A	8: 104,977,671 (GRCm39)	V8I	probably benign	Het
Cln5	A	G	14: 103,310,711 (GRCm39)	N126S	probably damaging	Het
Col5a2	A	T	1: 45,456,106 (GRCm39)	M327K	probably benign	Het
Col6a6	A	T	9: 105,644,528 (GRCm39)	F1253L	probably damaging	Het
Crb2	G	A	2: 37,685,395 (GRCm39)	S1169N	probably benign	Het
Cspg4	A	T	9: 56,790,967 (GRCm39)	D72V	probably damaging	Het
Ctnnd2	C	T	15: 30,905,873 (GRCm39)	P840L	probably damaging	Het
Eea1	T	C	10: 95,826,274 (GRCm39)	Y105H	probably damaging	Het
Epha4	A	C	1: 77,483,422 (GRCm39)	S196A	probably damaging	Het
Evx1	C	T	6: 52,293,746 (GRCm39)	R305C	probably damaging	Het
Fcamr	G	T	1: 130,740,328 (GRCm39)	G249V	probably damaging	Het
Fdxacb1	C	T	9: 50,681,451 (GRCm39)	T124I	probably damaging	Het
Fmn1	T	G	2: 113,355,914 (GRCm39)	L883R	unknown	Het
Frmd4b	T	C	6: 97,273,046 (GRCm39)	N782S	probably damaging	Het
Frmpd2	T	G	14: 33,248,380 (GRCm39)	C621W	probably damaging	Het
Fsip2	T	A	2: 82,816,984 (GRCm39)	V4239D	probably benign	Het
Gcnt4	A	G	13: 97,083,844 (GRCm39)	Y380C	probably damaging	Het
Gstp3	C	T	19: 4,108,145 (GRCm39)	V93M	possibly damaging	Het
Helz2	A	T	2: 180,874,920 (GRCm39)	M1858K	possibly damaging	Het
Ighv1-7	T	A	12: 114,502,353 (GRCm39)	K38M	probably damaging	Het
Klri1	T	C	6: 129,680,283 (GRCm39)	K124R	probably null	Het
Kmt2c	A	T	5: 25,514,609 (GRCm39)	F3078Y	probably damaging	Het
Map4k4	C	T	1: 40,058,770 (GRCm39)	P988L	probably damaging	Het
Mmrn1	G	A	6: 60,953,077 (GRCm39)	E453K	probably damaging	Het
Muc5b	T	A	7: 141,417,875 (GRCm39)	V3607E	probably benign	Het
Myo15a	T	A	11: 60,417,434 (GRCm39)	I3461N		Het
Ngef	G	A	1: 87,405,376 (GRCm39)	S678F	probably damaging	Het
Npc1l1	A	T	11: 6,178,157 (GRCm39)	S418T	probably benign	Het
Nprl2	A	G	9: 107,421,995 (GRCm39)	D257G	probably damaging	Het
Or4c113	A	T	2: 88,885,542 (GRCm39)	V76D	probably damaging	Het
Pard3b	A	G	1: 62,383,294 (GRCm39)	D796G	probably benign	Het
Pcf11	T	C	7: 92,302,451 (GRCm39)	T1220A	probably benign	Het
Pkhd1	T	C	1: 20,187,785 (GRCm39)	I3508V	possibly damaging	Het
Ppargc1a	A	T	5: 51,647,570 (GRCm39)	S258T	probably damaging	Het
Prom1	A	G	5: 44,159,135 (GRCm39)	Y830H	probably damaging	Het
Ptprm	T	A	17: 67,051,732 (GRCm39)	H1001L	probably damaging	Het
Rab8b	A	G	9: 66,826,807 (GRCm39)	F37L	probably benign	Het
Rsf1	GGCG	GGCGACGGCTGCG	7: 97,229,113 (GRCm39)		probably benign	Het
Rsf1	GGCGGCGGC	GGCGGCGGCCGCGGCGGC	7: 97,229,125 (GRCm39)		probably benign	Het
Sdk1	A	T	5: 142,070,278 (GRCm39)	I1148F	probably damaging	Het
Sec62	A	G	3: 30,864,621 (GRCm39)	K162E	unknown	Het
Serpinb13	A	T	1: 106,928,519 (GRCm39)	I380F	probably damaging	Het
Sptb	T	C	12: 76,634,186 (GRCm39)	D2175G	probably benign	Het
St3gal6	T	C	16: 58,314,095 (GRCm39)	I13V	probably benign	Het
Tfpi2	A	T	6: 3,967,996 (GRCm39)	L48H	probably damaging	Het
Tmem121b	T	A	6: 120,469,707 (GRCm39)	I337F	probably damaging	Het
Tpbgl	T	C	7: 99,275,182 (GRCm39)	D225G	probably benign	Het
Trpa1	A	G	1: 14,959,664 (GRCm39)	V674A	probably benign	Het
Trpa1	A	G	1: 14,963,563 (GRCm39)	L553P	probably damaging	Het
Tyrp1	T	C	4: 80,755,798 (GRCm39)	V189A	probably damaging	Het
Vmn1r19	G	T	6: 57,381,991 (GRCm39)	M181I	probably benign	Het
Vmn2r10	T	C	5: 109,149,791 (GRCm39)	T418A	probably benign	Het
Zfp617	A	G	8: 72,686,927 (GRCm39)	Y419C	probably damaging	Het

Other mutations in Gm7298

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0030:Gm7298	UTSW	6	121,751,009 (GRCm39)	missense	probably benign
R4978:Gm7298	UTSW	6	121,710,076 (GRCm39)	critical splice donor site	probably null
R4980:Gm7298	UTSW	6	121,736,198 (GRCm39)	splice site	probably null
R6000:Gm7298	UTSW	6	121,742,038 (GRCm39)	missense	possibly damaging	0.91
R6160:Gm7298	UTSW	6	121,741,886 (GRCm39)	missense	probably benign	0.28
R6180:Gm7298	UTSW	6	121,737,782 (GRCm39)	missense	probably benign	0.01
R6243:Gm7298	UTSW	6	121,756,096 (GRCm39)	missense	possibly damaging	0.88
R6266:Gm7298	UTSW	6	121,759,663 (GRCm39)	missense	probably damaging	1.00
R6268:Gm7298	UTSW	6	121,756,032 (GRCm39)	missense	possibly damaging	0.83
R6363:Gm7298	UTSW	6	121,765,565 (GRCm39)	missense	probably damaging	1.00
R6364:Gm7298	UTSW	6	121,756,402 (GRCm39)	missense	possibly damaging	0.90
R6527:Gm7298	UTSW	6	121,746,669 (GRCm39)	missense	probably benign	0.01
R6538:Gm7298	UTSW	6	121,753,132 (GRCm39)	missense	probably damaging	0.98
R6801:Gm7298	UTSW	6	121,752,768 (GRCm39)	missense	probably benign	0.03
R6884:Gm7298	UTSW	6	121,737,480 (GRCm39)	missense	possibly damaging	0.74
R6935:Gm7298	UTSW	6	121,744,653 (GRCm39)	missense	probably benign	0.02
R7051:Gm7298	UTSW	6	121,751,993 (GRCm39)	critical splice donor site	probably null
R7144:Gm7298	UTSW	6	121,738,546 (GRCm39)	missense	probably damaging	0.99
R7178:Gm7298	UTSW	6	121,762,855 (GRCm39)	missense	probably damaging	0.98
R7398:Gm7298	UTSW	6	121,758,912 (GRCm39)	missense	probably benign	0.02
R7706:Gm7298	UTSW	6	121,712,570 (GRCm39)	missense	probably damaging	0.96
R7793:Gm7298	UTSW	6	121,737,563 (GRCm39)	critical splice donor site	probably null
R7829:Gm7298	UTSW	6	121,742,297 (GRCm39)	missense	probably damaging	1.00
R7877:Gm7298	UTSW	6	121,759,741 (GRCm39)	nonsense	probably null
R8010:Gm7298	UTSW	6	121,712,542 (GRCm39)	missense	probably benign
R8167:Gm7298	UTSW	6	121,761,414 (GRCm39)	nonsense	probably null
R8188:Gm7298	UTSW	6	121,763,537 (GRCm39)	critical splice acceptor site	probably null
R8248:Gm7298	UTSW	6	121,764,402 (GRCm39)	missense	probably benign	0.02
R8669:Gm7298	UTSW	6	121,742,002 (GRCm39)	missense	probably benign
R8806:Gm7298	UTSW	6	121,761,641 (GRCm39)	synonymous	silent
R8867:Gm7298	UTSW	6	121,748,788 (GRCm39)	missense	probably benign
R8930:Gm7298	UTSW	6	121,742,030 (GRCm39)	missense	probably benign	0.01
R8932:Gm7298	UTSW	6	121,742,030 (GRCm39)	missense	probably benign	0.01
R8947:Gm7298	UTSW	6	121,757,553 (GRCm39)	missense	possibly damaging	0.62
R9016:Gm7298	UTSW	6	121,758,800 (GRCm39)	missense	possibly damaging	0.96
R9040:Gm7298	UTSW	6	121,764,438 (GRCm39)	missense	probably benign	0.20
R9069:Gm7298	UTSW	6	121,761,393 (GRCm39)	missense	probably benign
R9154:Gm7298	UTSW	6	121,756,436 (GRCm39)	missense	probably damaging	1.00
R9273:Gm7298	UTSW	6	121,756,604 (GRCm39)	intron	probably benign
R9371:Gm7298	UTSW	6	121,744,541 (GRCm39)	missense	probably benign	0.02
R9372:Gm7298	UTSW	6	121,748,746 (GRCm39)	missense	probably benign	0.21
R9490:Gm7298	UTSW	6	121,751,083 (GRCm39)	missense	probably benign	0.00
R9649:Gm7298	UTSW	6	121,764,491 (GRCm39)	missense	probably damaging	1.00
Z1176:Gm7298	UTSW	6	121,741,834 (GRCm39)	missense	possibly damaging	0.48
Z1176:Gm7298	UTSW	6	121,741,829 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GCACAATAAATGGTTCCTTTGTCTG -3'
(R):5'- GCCTGAACTGTGTGAATCTGG -3'

Sequencing Primer
(F):5'- CCCTCTTACCTGGTATAAATATTTGC -3'
(R):5'- AACTGTGTGAATCTGGTTGCAG -3'

Posted On

2021-08-02