Mutagenetix > Incidental Mutation

Incidental Mutation 'R8911:Hectd1'

678644

Institutional Source

Beutler Lab

Gene Symbol

Hectd1

Ensembl Gene

ENSMUSG00000035247

Gene Name

HECT domain E3 ubiquitin protein ligase 1

Synonyms

A630086P08Rik, b2b327Clo, opm

MMRRC Submission

068764-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8911 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

51790505-51876319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 51795616 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 2271 (I2271T)

Ref Sequence

ENSEMBL: ENSMUSP00000136449 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042052] [ENSMUST00000179265] [ENSMUST00000218820]

AlphaFold

Q69ZR2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042052
AA Change: I2266T

PolyPhen 2 Score 0.580 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000046766
Gene: ENSMUSG00000035247
AA Change: I2266T

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	395	424	1.44e-1	SMART
ANK	426	455	2.81e-4	SMART
ANK	459	488	1.55e2	SMART
low complexity region	490	509	N/A	INTRINSIC
low complexity region	630	654	N/A	INTRINSIC
low complexity region	707	723	N/A	INTRINSIC
low complexity region	821	832	N/A	INTRINSIC
Pfam:Sad1_UNC	1107	1240	9.2e-27	PFAM
low complexity region	1259	1271	N/A	INTRINSIC
Pfam:MIB_HERC2	1277	1338	7.6e-27	PFAM
low complexity region	1373	1401	N/A	INTRINSIC
low complexity region	1441	1458	N/A	INTRINSIC
low complexity region	1484	1495	N/A	INTRINSIC
low complexity region	1508	1524	N/A	INTRINSIC
low complexity region	1600	1630	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
low complexity region	1674	1703	N/A	INTRINSIC
low complexity region	1745	1752	N/A	INTRINSIC
PDB:2LC3\|A	1879	1966	4e-57	PDB
low complexity region	2101	2117	N/A	INTRINSIC
HECTc	2143	2610	8.32e-76	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000179265
AA Change: I2271T

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000136449
Gene: ENSMUSG00000035247
AA Change: I2271T

Domain	Start	End	E-Value	Type
low complexity region	317	331	N/A	INTRINSIC
ANK	396	425	1.44e-1	SMART
ANK	427	456	2.81e-4	SMART
ANK	460	489	1.55e2	SMART
low complexity region	491	510	N/A	INTRINSIC
low complexity region	631	655	N/A	INTRINSIC
low complexity region	708	724	N/A	INTRINSIC
low complexity region	822	833	N/A	INTRINSIC
Pfam:Sad1_UNC	1112	1245	1.3e-26	PFAM
low complexity region	1264	1276	N/A	INTRINSIC
Pfam:MIB_HERC2	1282	1341	5.3e-26	PFAM
low complexity region	1378	1406	N/A	INTRINSIC
low complexity region	1446	1463	N/A	INTRINSIC
low complexity region	1489	1500	N/A	INTRINSIC
low complexity region	1513	1529	N/A	INTRINSIC
low complexity region	1605	1635	N/A	INTRINSIC
low complexity region	1638	1656	N/A	INTRINSIC
low complexity region	1679	1708	N/A	INTRINSIC
low complexity region	1750	1757	N/A	INTRINSIC
PDB:2LC3\|A	1884	1971	3e-57	PDB
low complexity region	2106	2122	N/A	INTRINSIC
HECTc	2148	2618	4.5e-72	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000218820

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (76/76)

MGI Phenotype

PHENOTYPE: Mice that are homozygous for either a gene trapped or an ENU-induced allele exhibit exencephaly associated with impaired head mesenchyme development and neural tube closure, and show eye and cranial vault dysplasia. Homozygotes for another ENU-induced allele show congenital cardiovascular defects. [provided by MGI curators]

Allele List at MGI

All alleles(30) : Gene trapped(29) Chemically induced(1)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	T	13: 77,429,234 (GRCm39)	I774F	probably benign	Het
3425401B19Rik	A	T	14: 32,383,626 (GRCm39)	C780S	possibly damaging	Het
Abca12	T	C	1: 71,380,690 (GRCm39)	D306G	probably benign	Het
Adgrl2	T	C	3: 148,558,163 (GRCm39)		probably benign	Het
Agt	T	C	8: 125,291,184 (GRCm39)	Y41C	probably benign	Het
Alx3	G	T	3: 107,511,603 (GRCm39)	R204L	probably damaging	Het
Arih2	C	G	9: 108,488,938 (GRCm39)	R260P	probably damaging	Het
Bcl11a	C	T	11: 24,114,763 (GRCm39)	P702L	probably damaging	Het
Bpifa2	A	G	2: 153,851,090 (GRCm39)	N17S	probably benign	Het
Cabcoco1	T	C	10: 68,377,584 (GRCm39)	D35G	probably benign	Het
Cd69	T	C	6: 129,252,187 (GRCm39)	K21R	probably benign	Het
Cdhr18	T	C	14: 13,823,796 (GRCm38)		probably null	Het
Cntn4	T	C	6: 106,330,743 (GRCm39)		probably null	Het
Cntnap5c	A	C	17: 58,506,043 (GRCm39)	N689T	probably damaging	Het
Cog5	A	G	12: 31,883,238 (GRCm39)	Y389C	probably damaging	Het
Col5a1	T	C	2: 27,887,630 (GRCm39)		probably null	Het
Coro1b	G	A	19: 4,200,803 (GRCm39)	R245Q	probably damaging	Het
Csmd1	T	C	8: 16,748,019 (GRCm39)	D244G	probably damaging	Het
Ctcfl	C	T	2: 172,937,121 (GRCm39)		probably null	Het
Dpy19l4	G	A	4: 11,317,078 (GRCm39)	P40L	possibly damaging	Het
Dsg4	C	A	18: 20,584,929 (GRCm39)	Y214*	probably null	Het
Eapp	A	T	12: 54,739,440 (GRCm39)		probably benign	Het
Fmo6	T	C	1: 162,748,114 (GRCm39)	T317A	possibly damaging	Het
Gm11595	A	G	11: 99,663,564 (GRCm39)	C39R	unknown	Het
Golim4	T	C	3: 75,813,703 (GRCm39)		probably benign	Het
Greb1	G	A	12: 16,740,903 (GRCm39)	S1393L	possibly damaging	Het
Guca1b	A	G	17: 47,700,044 (GRCm39)	I73V	probably benign	Het
Hdac7	C	T	15: 97,694,789 (GRCm39)	V796I	possibly damaging	Het
Heg1	T	G	16: 33,558,627 (GRCm39)	Y1066*	probably null	Het
Helz2	C	A	2: 180,880,173 (GRCm39)	K514N		Het
Ice1	C	T	13: 70,740,787 (GRCm39)	R70Q		Het
Il18rap	C	T	1: 40,582,177 (GRCm39)	T366M	probably benign	Het
Itgae	A	G	11: 73,004,447 (GRCm39)	T245A	probably damaging	Het
Jhy	G	A	9: 40,822,453 (GRCm39)	Q562*	probably null	Het
Khnyn	A	G	14: 56,124,735 (GRCm39)	R330G	probably benign	Het
Lamc2	A	T	1: 153,027,873 (GRCm39)	C184S	probably damaging	Het
Lhx5	T	A	5: 120,574,509 (GRCm39)	L271*	probably null	Het
Magel2	G	A	7: 62,029,537 (GRCm39)	V814M	unknown	Het
Manf	A	G	9: 106,767,461 (GRCm39)	I85T	possibly damaging	Het
Mgmt	A	G	7: 136,729,794 (GRCm39)	T203A	probably benign	Het
Mmp9	C	T	2: 164,794,568 (GRCm39)	S520F	possibly damaging	Het
Myrip	A	G	9: 120,270,484 (GRCm39)	E578G	possibly damaging	Het
Naaladl2	A	G	3: 23,900,757 (GRCm39)	M691T	probably damaging	Het
Ncln	C	T	10: 81,323,519 (GRCm39)	V51I	probably benign	Het
Or2n1d	A	T	17: 38,646,320 (GRCm39)	T91S	possibly damaging	Het
Or4c112	T	C	2: 88,854,294 (GRCm39)	N18D	probably benign	Het
Or4c117	T	C	2: 88,955,608 (GRCm39)	I156V	probably benign	Het
Pcdhga12	A	T	18: 37,900,118 (GRCm39)	M317L	possibly damaging	Het
Pcnt	T	G	10: 76,223,359 (GRCm39)	K1941T	probably damaging	Het
Pde4dip	A	T	3: 97,650,917 (GRCm39)	M896K	probably benign	Het
Pfkfb3	A	T	2: 11,487,254 (GRCm39)		probably null	Het
Pik3cb	A	G	9: 98,946,201 (GRCm39)	S542P	probably benign	Het
Pik3cg	A	G	12: 32,247,257 (GRCm39)	V822A	probably benign	Het
Plcl2	G	T	17: 50,915,141 (GRCm39)	G717C	probably damaging	Het
Ptprs	C	T	17: 56,730,320 (GRCm39)	A1185T	probably benign	Het
Ptpru	T	A	4: 131,503,560 (GRCm39)	I1157F	probably damaging	Het
Rbbp6	A	G	7: 122,591,268 (GRCm39)	T457A	possibly damaging	Het
Rdh16f2	A	G	10: 127,712,812 (GRCm39)	E270G	probably damaging	Het
Rrp15	C	T	1: 186,453,641 (GRCm39)	E269K	unknown	Het
Sec23b	T	C	2: 144,401,316 (GRCm39)	V59A	probably benign	Het
Slc22a21	A	T	11: 53,846,809 (GRCm39)		probably null	Het
Sp5	C	A	2: 70,306,962 (GRCm39)	P216T	probably benign	Het
Synj1	A	T	16: 90,775,622 (GRCm39)	D385E	probably damaging	Het
Trim37	C	T	11: 87,097,629 (GRCm39)	S808F	possibly damaging	Het
Tsks	A	T	7: 44,592,694 (GRCm39)		probably benign	Het
Upf1	A	T	8: 70,791,087 (GRCm39)	S563T	possibly damaging	Het
Upf2	A	G	2: 5,987,893 (GRCm39)	D580G	unknown	Het
Usp14	A	G	18: 9,996,194 (GRCm39)	I462T	probably damaging	Het
Usp48	G	A	4: 137,340,996 (GRCm39)	G332E	probably benign	Het
Vmn1r184	C	G	7: 25,966,310 (GRCm39)	Q19E	possibly damaging	Het
Vps50	C	A	6: 3,516,710 (GRCm39)	A64E	probably benign	Het
Zc3h11a	T	A	1: 133,566,339 (GRCm39)	N211I	probably damaging	Het
Zfp518a	A	C	19: 40,901,870 (GRCm39)	K600Q	possibly damaging	Het
Zfp729a	A	T	13: 67,768,061 (GRCm39)	S723T	probably benign	Het
Zmynd12	A	T	4: 119,294,286 (GRCm39)	I88F	probably damaging	Het

Other mutations in Hectd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Hectd1	APN	12	51,806,215 (GRCm39)	missense	probably benign
IGL00402:Hectd1	APN	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
IGL00419:Hectd1	APN	12	51,810,818 (GRCm39)	missense	probably damaging	0.99
IGL00518:Hectd1	APN	12	51,823,272 (GRCm39)	splice site	probably benign
IGL00565:Hectd1	APN	12	51,837,181 (GRCm39)	missense	probably damaging	0.97
IGL00574:Hectd1	APN	12	51,820,787 (GRCm39)	missense	probably benign	0.17
IGL00576:Hectd1	APN	12	51,806,092 (GRCm39)	missense	probably damaging	0.99
IGL00788:Hectd1	APN	12	51,795,571 (GRCm39)	missense	probably damaging	0.99
IGL00978:Hectd1	APN	12	51,838,173 (GRCm39)	missense	possibly damaging	0.95
IGL01328:Hectd1	APN	12	51,807,904 (GRCm39)	missense	probably damaging	1.00
IGL01337:Hectd1	APN	12	51,849,057 (GRCm39)	missense	possibly damaging	0.95
IGL01634:Hectd1	APN	12	51,850,562 (GRCm39)	missense	probably damaging	0.98
IGL01731:Hectd1	APN	12	51,849,593 (GRCm39)	missense	possibly damaging	0.59
IGL01920:Hectd1	APN	12	51,829,337 (GRCm39)	missense	probably damaging	0.99
IGL01951:Hectd1	APN	12	51,841,280 (GRCm39)	nonsense	probably null
IGL01994:Hectd1	APN	12	51,844,725 (GRCm39)	missense	probably damaging	0.99
IGL02140:Hectd1	APN	12	51,820,920 (GRCm39)	missense	probably damaging	0.99
IGL02150:Hectd1	APN	12	51,815,974 (GRCm39)	missense	probably damaging	0.97
IGL02156:Hectd1	APN	12	51,800,916 (GRCm39)	splice site	probably benign
IGL02177:Hectd1	APN	12	51,819,103 (GRCm39)	missense	probably damaging	0.99
IGL02502:Hectd1	APN	12	51,844,635 (GRCm39)	missense	possibly damaging	0.77
IGL02505:Hectd1	APN	12	51,847,496 (GRCm39)	critical splice donor site	probably null
IGL02519:Hectd1	APN	12	51,815,894 (GRCm39)	missense	probably damaging	0.99
IGL02624:Hectd1	APN	12	51,809,233 (GRCm39)	missense	possibly damaging	0.61
IGL02833:Hectd1	APN	12	51,810,864 (GRCm39)	missense	probably damaging	0.96
IGL02851:Hectd1	APN	12	51,814,423 (GRCm39)	missense	possibly damaging	0.94
IGL02866:Hectd1	APN	12	51,837,396 (GRCm39)	missense	probably damaging	1.00
IGL02981:Hectd1	APN	12	51,815,670 (GRCm39)	missense	possibly damaging	0.70
IGL02987:Hectd1	APN	12	51,791,550 (GRCm39)	missense	probably damaging	1.00
IGL02999:Hectd1	APN	12	51,874,205 (GRCm39)	missense	possibly damaging	0.77
IGL03071:Hectd1	APN	12	51,815,957 (GRCm39)	missense	probably benign	0.00
IGL03078:Hectd1	APN	12	51,849,019 (GRCm39)	missense	probably damaging	0.98
IGL03299:Hectd1	APN	12	51,847,671 (GRCm39)	splice site	probably benign
3-1:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R0039:Hectd1	UTSW	12	51,800,608 (GRCm39)	missense	possibly damaging	0.83
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0238:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0239:Hectd1	UTSW	12	51,816,101 (GRCm39)	missense	possibly damaging	0.72
R0268:Hectd1	UTSW	12	51,815,891 (GRCm39)	missense	possibly damaging	0.94
R0268:Hectd1	UTSW	12	51,815,890 (GRCm39)	missense	probably damaging	0.99
R0409:Hectd1	UTSW	12	51,829,339 (GRCm39)	missense	possibly damaging	0.59
R1019:Hectd1	UTSW	12	51,795,440 (GRCm39)	missense	probably damaging	0.99
R1072:Hectd1	UTSW	12	51,807,855 (GRCm39)	missense	probably benign	0.11
R1087:Hectd1	UTSW	12	51,823,355 (GRCm39)	missense	probably damaging	0.99
R1165:Hectd1	UTSW	12	51,810,947 (GRCm39)	splice site	probably benign
R1350:Hectd1	UTSW	12	51,809,217 (GRCm39)	missense	probably benign
R1553:Hectd1	UTSW	12	51,820,661 (GRCm39)	missense	probably damaging	0.98
R1666:Hectd1	UTSW	12	51,800,607 (GRCm39)	missense	possibly damaging	0.91
R1676:Hectd1	UTSW	12	51,791,571 (GRCm39)	missense	probably damaging	1.00
R1694:Hectd1	UTSW	12	51,791,375 (GRCm39)	missense	probably damaging	1.00
R1778:Hectd1	UTSW	12	51,800,590 (GRCm39)	missense	probably damaging	0.99
R1856:Hectd1	UTSW	12	51,791,577 (GRCm39)	missense	probably damaging	1.00
R1859:Hectd1	UTSW	12	51,853,350 (GRCm39)	missense	probably damaging	1.00
R1884:Hectd1	UTSW	12	51,847,738 (GRCm39)	missense	probably benign	0.00
R1982:Hectd1	UTSW	12	51,832,624 (GRCm39)	missense	probably damaging	0.97
R2034:Hectd1	UTSW	12	51,803,899 (GRCm39)	splice site	probably null
R2061:Hectd1	UTSW	12	51,841,227 (GRCm39)	missense	probably damaging	0.99
R2078:Hectd1	UTSW	12	51,795,325 (GRCm39)	missense	probably damaging	0.99
R2176:Hectd1	UTSW	12	51,792,277 (GRCm39)	missense	probably damaging	1.00
R2210:Hectd1	UTSW	12	51,853,245 (GRCm39)	missense	probably damaging	0.99
R2248:Hectd1	UTSW	12	51,853,254 (GRCm39)	missense	probably damaging	0.99
R2282:Hectd1	UTSW	12	51,815,791 (GRCm39)	missense	possibly damaging	0.95
R2402:Hectd1	UTSW	12	51,792,317 (GRCm39)	missense	probably benign	0.01
R3876:Hectd1	UTSW	12	51,815,513 (GRCm39)	missense	probably damaging	0.98
R4027:Hectd1	UTSW	12	51,849,219 (GRCm39)	critical splice acceptor site	probably null
R4085:Hectd1	UTSW	12	51,821,533 (GRCm39)	missense	possibly damaging	0.93
R4115:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4116:Hectd1	UTSW	12	51,815,506 (GRCm39)	nonsense	probably null
R4169:Hectd1	UTSW	12	51,837,008 (GRCm39)	missense	probably damaging	0.97
R4434:Hectd1	UTSW	12	51,798,835 (GRCm39)	missense	probably damaging	1.00
R4507:Hectd1	UTSW	12	51,837,276 (GRCm39)	missense	probably damaging	0.97
R4578:Hectd1	UTSW	12	51,798,715 (GRCm39)	missense	probably damaging	1.00
R4579:Hectd1	UTSW	12	51,791,356 (GRCm39)	missense	probably damaging	0.97
R4709:Hectd1	UTSW	12	51,834,695 (GRCm39)	missense	possibly damaging	0.94
R4812:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R4883:Hectd1	UTSW	12	51,831,030 (GRCm39)	nonsense	probably null
R4885:Hectd1	UTSW	12	51,847,505 (GRCm39)	missense	probably damaging	0.97
R4975:Hectd1	UTSW	12	51,809,280 (GRCm39)	missense	probably benign	0.02
R4983:Hectd1	UTSW	12	51,831,045 (GRCm39)	missense	probably benign	0.01
R5007:Hectd1	UTSW	12	51,849,443 (GRCm39)	missense	possibly damaging	0.95
R5046:Hectd1	UTSW	12	51,797,171 (GRCm39)	missense	probably damaging	1.00
R5062:Hectd1	UTSW	12	51,791,662 (GRCm39)	missense	probably damaging	0.98
R5164:Hectd1	UTSW	12	51,874,272 (GRCm39)	start codon destroyed	probably null	0.60
R5213:Hectd1	UTSW	12	51,849,316 (GRCm39)	critical splice donor site	probably null
R5535:Hectd1	UTSW	12	51,849,109 (GRCm39)	missense	probably damaging	0.98
R5776:Hectd1	UTSW	12	51,810,897 (GRCm39)	missense	possibly damaging	0.91
R5846:Hectd1	UTSW	12	51,820,618 (GRCm39)	missense	probably damaging	0.99
R5907:Hectd1	UTSW	12	51,845,537 (GRCm39)	missense	probably damaging	0.98
R5911:Hectd1	UTSW	12	51,849,035 (GRCm39)	missense	probably damaging	0.99
R5919:Hectd1	UTSW	12	51,815,855 (GRCm39)	missense	probably damaging	0.98
R6051:Hectd1	UTSW	12	51,800,887 (GRCm39)	missense	probably benign
R6141:Hectd1	UTSW	12	51,792,875 (GRCm39)	critical splice donor site	probably null
R6172:Hectd1	UTSW	12	51,816,065 (GRCm39)	missense	probably damaging	1.00
R6194:Hectd1	UTSW	12	51,795,228 (GRCm39)	missense	probably damaging	0.99
R6356:Hectd1	UTSW	12	51,791,402 (GRCm39)	missense	probably damaging	1.00
R6795:Hectd1	UTSW	12	51,841,270 (GRCm39)	missense	possibly damaging	0.94
R6909:Hectd1	UTSW	12	51,810,945 (GRCm39)	splice site	probably null
R6971:Hectd1	UTSW	12	51,795,526 (GRCm39)	nonsense	probably null
R7079:Hectd1	UTSW	12	51,834,638 (GRCm39)	missense	possibly damaging	0.96
R7104:Hectd1	UTSW	12	51,874,134 (GRCm39)	critical splice donor site	probably null
R7171:Hectd1	UTSW	12	51,806,080 (GRCm39)	missense	probably damaging	0.99
R7296:Hectd1	UTSW	12	51,832,635 (GRCm39)	missense	possibly damaging	0.73
R7346:Hectd1	UTSW	12	51,797,104 (GRCm39)	missense	probably benign
R7355:Hectd1	UTSW	12	51,838,081 (GRCm39)	missense	possibly damaging	0.72
R7468:Hectd1	UTSW	12	51,791,588 (GRCm39)	splice site	probably null
R7531:Hectd1	UTSW	12	51,853,150 (GRCm39)	missense	probably benign	0.33
R7532:Hectd1	UTSW	12	51,837,233 (GRCm39)	missense	probably damaging	0.98
R7755:Hectd1	UTSW	12	51,849,003 (GRCm39)	missense	possibly damaging	0.86
R7807:Hectd1	UTSW	12	51,792,171 (GRCm39)	missense	probably damaging	1.00
R7842:Hectd1	UTSW	12	51,819,343 (GRCm39)	missense	probably damaging	0.99
R7922:Hectd1	UTSW	12	51,836,978 (GRCm39)	nonsense	probably null
R8059:Hectd1	UTSW	12	51,837,161 (GRCm39)	missense	possibly damaging	0.53
R8085:Hectd1	UTSW	12	51,795,679 (GRCm39)	missense	probably damaging	0.97
R8145:Hectd1	UTSW	12	51,831,016 (GRCm39)	missense	possibly damaging	0.72
R8157:Hectd1	UTSW	12	51,838,073 (GRCm39)	missense	possibly damaging	0.53
R8405:Hectd1	UTSW	12	51,874,178 (GRCm39)	missense	probably benign	0.01
R8505:Hectd1	UTSW	12	51,797,145 (GRCm39)	missense	probably damaging	1.00
R8511:Hectd1	UTSW	12	51,834,654 (GRCm39)	missense	probably benign	0.01
R8697:Hectd1	UTSW	12	51,819,320 (GRCm39)	critical splice donor site	probably benign
R8725:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8727:Hectd1	UTSW	12	51,849,000 (GRCm39)	missense	possibly damaging	0.92
R8983:Hectd1	UTSW	12	51,791,410 (GRCm39)	missense	probably damaging	0.97
R9037:Hectd1	UTSW	12	51,832,665 (GRCm39)	missense	possibly damaging	0.85
R9219:Hectd1	UTSW	12	51,800,612 (GRCm39)	missense	probably damaging	0.99
R9413:Hectd1	UTSW	12	51,792,880 (GRCm39)	nonsense	probably null
R9456:Hectd1	UTSW	12	51,832,584 (GRCm39)	missense	probably benign
R9513:Hectd1	UTSW	12	51,816,079 (GRCm39)	missense	possibly damaging	0.92
R9640:Hectd1	UTSW	12	51,795,197 (GRCm39)	nonsense	probably null
R9641:Hectd1	UTSW	12	51,816,047 (GRCm39)	missense	probably benign	0.00
R9713:Hectd1	UTSW	12	51,823,328 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ATCCCATTAAACCAGGCTGGG -3'
(R):5'- TGGAGGTGGATTGAAGCCTC -3'

Sequencing Primer
(F):5'- CTTAGGGGGATCGAGGATAAATTCTG -3'
(R):5'- TGGATTGAAGCCTCCAGGATAC -3'

Posted On

2021-08-02