Incidental Mutation 'R8914:Ccnl1'
ID 678803
Institutional Source Beutler Lab
Gene Symbol Ccnl1
Ensembl Gene ENSMUSG00000027829
Gene Name cyclin L1
Synonyms ania-6a, 2610030E23Rik
MMRRC Submission 068766-MU
Accession Numbers
Essential gene? Possibly essential (E-score: 0.712) question?
Stock # R8914 (G1)
Quality Score 225.009
Status Validated
Chromosome 3
Chromosomal Location 65853572-65865670 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 65854080 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Threonine at position 514 (S514T)
Ref Sequence ENSEMBL: ENSMUSP00000029416 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029416] [ENSMUST00000129002] [ENSMUST00000135719] [ENSMUST00000154585]
AlphaFold Q52KE7
Predicted Effect unknown
Transcript: ENSMUST00000029416
AA Change: S514T
SMART Domains Protein: ENSMUSP00000029416
Gene: ENSMUSG00000027829
AA Change: S514T

DomainStartEndE-ValueType
low complexity region 2 45 N/A INTRINSIC
CYCLIN 94 196 3.55e-11 SMART
Cyclin_C 205 320 7.79e-5 SMART
CYCLIN 209 293 9.01e-13 SMART
low complexity region 386 445 N/A INTRINSIC
low complexity region 464 485 N/A INTRINSIC
low complexity region 494 532 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000129002
SMART Domains Protein: ENSMUSP00000118377
Gene: ENSMUSG00000027829

DomainStartEndE-ValueType
low complexity region 2 45 N/A INTRINSIC
CYCLIN 94 174 3.93e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000135719
SMART Domains Protein: ENSMUSP00000118394
Gene: ENSMUSG00000027829

DomainStartEndE-ValueType
low complexity region 2 13 N/A INTRINSIC
CYCLIN 62 142 3.93e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000144810
Predicted Effect probably benign
Transcript: ENSMUST00000145186
Predicted Effect probably benign
Transcript: ENSMUST00000154585
SMART Domains Protein: ENSMUSP00000119229
Gene: ENSMUSG00000027829

DomainStartEndE-ValueType
low complexity region 2 45 N/A INTRINSIC
CYCLIN 94 174 3.93e-4 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 100% (62/62)
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
5330417H12Rik C T 7: 107,223,922 (GRCm39) E51K unknown Het
Akr1c20 T C 13: 4,561,215 (GRCm39) D146G probably benign Het
Anapc4 T A 5: 53,000,843 (GRCm39) C205* probably null Het
Ap3s2 A G 7: 79,564,911 (GRCm39) I86T probably benign Het
Arih2 C G 9: 108,488,938 (GRCm39) R260P probably damaging Het
Atp9a C T 2: 168,479,420 (GRCm39) probably null Het
Bcl11b C A 12: 107,883,163 (GRCm39) R384L probably damaging Het
Bltp3a T G 17: 28,105,887 (GRCm39) D804E possibly damaging Het
Brca2 T A 5: 150,465,208 (GRCm39) D1657E probably damaging Het
Cabyr C A 18: 12,884,077 (GRCm39) T188N probably damaging Het
Capn5 C A 7: 97,784,997 (GRCm39) W159L probably damaging Het
Ccdc121rt3 A T 5: 112,503,087 (GRCm39) Y206N probably damaging Het
Cdk14 G T 5: 5,086,515 (GRCm39) H286Q possibly damaging Het
Cpsf3 T A 12: 21,345,112 (GRCm39) H141Q probably damaging Het
Ddx24 A G 12: 103,390,665 (GRCm39) S109P possibly damaging Het
Dnmt3a A T 12: 3,916,192 (GRCm39) R126* probably null Het
Dpys C A 15: 39,720,619 (GRCm39) R47L probably benign Het
Dync1li2 A G 8: 105,152,090 (GRCm39) V361A probably benign Het
Epha7 T G 4: 28,963,892 (GRCm39) D962E probably damaging Het
Erh G T 12: 80,684,282 (GRCm39) A65E probably benign Het
Fyb2 T C 4: 104,857,700 (GRCm39) S626P probably benign Het
Gtpbp1 T A 15: 79,600,393 (GRCm39) V442D probably damaging Het
Hecw1 T A 13: 14,422,188 (GRCm39) I973F probably damaging Het
Igkv1-99 A C 6: 68,519,340 (GRCm39) K99T Het
Igkv6-14 C T 6: 70,412,180 (GRCm39) V35I probably benign Het
Il18rap C T 1: 40,582,177 (GRCm39) T366M probably benign Het
Klk1b9 C T 7: 43,628,925 (GRCm39) T143I possibly damaging Het
Krt1 AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC 15: 101,758,813 (GRCm39) probably benign Het
Mettl15 A C 2: 108,967,625 (GRCm39) probably benign Het
Mki67 A G 7: 135,299,595 (GRCm39) V1813A Het
Mrps30 A G 13: 118,523,755 (GRCm39) Y6H possibly damaging Het
Mthfd1 T A 12: 76,329,710 (GRCm39) I176K probably benign Het
Nckap5l C T 15: 99,323,761 (GRCm39) G914D probably damaging Het
Nemf C A 12: 69,363,089 (GRCm39) E930* probably null Het
Nuggc G A 14: 65,879,354 (GRCm39) V705I probably benign Het
Omd A T 13: 49,745,718 (GRCm39) K376M probably damaging Het
Or10a3n T C 7: 108,492,736 (GRCm39) R298G probably damaging Het
Or2n1d A T 17: 38,646,320 (GRCm39) T91S possibly damaging Het
Or4k42 C A 2: 111,320,004 (GRCm39) L166F probably damaging Het
Or51l14 T A 7: 103,101,090 (GRCm39) L182Q probably damaging Het
Or5g27 A G 2: 85,410,056 (GRCm39) T158A possibly damaging Het
Or5p68 A G 7: 107,945,759 (GRCm39) V143A probably benign Het
Or6c200-ps1 C A 10: 128,871,372 (GRCm39) M2I probably benign Het
Pi15 A G 1: 17,691,962 (GRCm39) Y217C probably damaging Het
Pou5f1 T C 17: 35,821,371 (GRCm39) S328P probably benign Het
Pparg T A 6: 115,440,133 (GRCm39) S236T probably benign Het
Prss23 T C 7: 89,159,854 (GRCm39) T72A probably benign Het
Ptprb C A 10: 116,158,567 (GRCm39) S546* probably null Het
Ranbp9 A G 13: 43,578,560 (GRCm39) I171T probably benign Het
Rap1gap2 A T 11: 74,326,587 (GRCm39) L160* probably null Het
Rfxap T C 3: 54,715,042 (GRCm39) T19A probably benign Het
Sctr T A 1: 119,959,386 (GRCm39) F95I probably benign Het
Sf3b3 T A 8: 111,540,439 (GRCm39) Q994L probably benign Het
Slfn9 A T 11: 82,872,132 (GRCm39) I868N probably damaging Het
Top3a A T 11: 60,631,405 (GRCm39) C986S probably damaging Het
Trak1 T A 9: 121,272,847 (GRCm39) L245Q unknown Het
Trim27 A G 13: 21,364,993 (GRCm39) D110G possibly damaging Het
Ubxn2a T C 12: 4,930,754 (GRCm39) D202G probably benign Het
Urb1 T C 16: 90,607,122 (GRCm39) K36R probably damaging Het
Usp34 A G 11: 23,293,604 (GRCm39) T252A Het
Vmn1r73 C A 7: 11,490,328 (GRCm39) H49N probably damaging Het
Vmn2r117 C T 17: 23,679,143 (GRCm39) V694I probably benign Het
Vmn2r26 T C 6: 124,038,983 (GRCm39) F853L probably benign Het
Vmn2r55 A T 7: 12,405,024 (GRCm39) H126Q probably benign Het
Other mutations in Ccnl1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01969:Ccnl1 APN 3 65,855,908 (GRCm39) splice site probably benign
IGL02353:Ccnl1 APN 3 65,856,141 (GRCm39) missense probably damaging 1.00
IGL02360:Ccnl1 APN 3 65,856,141 (GRCm39) missense probably damaging 1.00
IGL02454:Ccnl1 APN 3 65,864,318 (GRCm39) missense probably damaging 0.99
R1598:Ccnl1 UTSW 3 65,854,191 (GRCm39) missense probably damaging 0.97
R1903:Ccnl1 UTSW 3 65,854,332 (GRCm39) missense possibly damaging 0.73
R2392:Ccnl1 UTSW 3 65,856,173 (GRCm39) missense probably damaging 1.00
R3879:Ccnl1 UTSW 3 65,856,179 (GRCm39) missense possibly damaging 0.85
R4607:Ccnl1 UTSW 3 65,854,131 (GRCm39) utr 3 prime probably benign
R4608:Ccnl1 UTSW 3 65,854,131 (GRCm39) utr 3 prime probably benign
R4739:Ccnl1 UTSW 3 65,854,092 (GRCm39) utr 3 prime probably benign
R4885:Ccnl1 UTSW 3 65,864,320 (GRCm39) missense probably damaging 1.00
R5833:Ccnl1 UTSW 3 65,855,922 (GRCm39) missense probably benign 0.23
R5933:Ccnl1 UTSW 3 65,855,763 (GRCm39) missense probably damaging 1.00
R6933:Ccnl1 UTSW 3 65,855,373 (GRCm39) missense probably benign 0.00
R7425:Ccnl1 UTSW 3 65,856,179 (GRCm39) missense probably damaging 1.00
R7943:Ccnl1 UTSW 3 65,864,326 (GRCm39) missense probably benign 0.30
R7988:Ccnl1 UTSW 3 65,865,282 (GRCm39) missense possibly damaging 0.64
R7990:Ccnl1 UTSW 3 65,854,314 (GRCm39) missense possibly damaging 0.73
R8137:Ccnl1 UTSW 3 65,865,291 (GRCm39) missense possibly damaging 0.96
R8690:Ccnl1 UTSW 3 65,855,165 (GRCm39) missense possibly damaging 0.73
R8736:Ccnl1 UTSW 3 65,865,447 (GRCm39) missense unknown
R8865:Ccnl1 UTSW 3 65,854,269 (GRCm39) missense probably benign 0.18
R9186:Ccnl1 UTSW 3 65,865,426 (GRCm39) missense unknown
R9612:Ccnl1 UTSW 3 65,865,404 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCAAAGAAAATGTCCTCAGTGTTTC -3'
(R):5'- AGCCCTCGAAGACATCATAATCATG -3'

Sequencing Primer
(F):5'- CACTGCATATGGGCAACA -3'
(R):5'- CGAAGACATCATAATCATGGTTCTCC -3'
Posted On 2021-08-02