Mutagenetix > Incidental Mutation

Incidental Mutation 'R8734:Cyp2b9'

679801

Institutional Source

Beutler Lab

Gene Symbol

Cyp2b9

Ensembl Gene

ENSMUSG00000040660

Gene Name

cytochrome P450, family 2, subfamily b, polypeptide 9

Synonyms

16\alphaoh-a, phenobarbitol inducible, type a, Cyp2b

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.098) question?

Stock #

R8734 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

26173411-26210661 bp(+) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

A to G at 26198610 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000080846 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082214]

AlphaFold

P12790

Predicted Effect

probably benign
Transcript: ENSMUST00000082214

SMART Domains

Protein: ENSMUSP00000080846
Gene: ENSMUSG00000040660

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:p450	31	488	1.7e-146	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (61/61)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc2	T	A	19: 43,782,416	C5S	probably damaging	Het
Actl6a	G	A	3: 32,719,955	D275N	probably benign	Het
Ahctf1	C	A	1: 179,780,865	E681*	probably null	Het
Ankub1	G	A	3: 57,692,285	S21L	probably benign	Het
Apmap	T	A	2: 150,588,904	K178N	probably benign	Het
Arhgap15	T	A	2: 44,243,118	N345K	probably damaging	Het
Armc8	A	G	9: 99,520,485	V379A	probably benign	Het
Atmin	G	A	8: 116,954,786	D175N	possibly damaging	Het
Atp5h	T	C	11: 115,416,863	E94G	possibly damaging	Het
B3gnt3	G	A	8: 71,693,501	T128M	probably damaging	Het
Ccdc88c	T	A	12: 100,940,135	T1040S	probably damaging	Het
Crot	C	T	5: 8,978,208	R247Q	probably benign	Het
Dcaf8	T	C	1: 172,193,860	W540R	probably benign	Het
Galnt11	T	C	5: 25,250,224	I186T	possibly damaging	Het
Gne	T	A	4: 44,072,911		probably benign	Het
Gpr37	A	T	6: 25,688,202	F299I	probably benign	Het
Gpr37l1	C	T	1: 135,167,429	A26T	probably benign	Het
Grm4	A	G	17: 27,438,791	Y414H	probably damaging	Het
Kcnh1	T	C	1: 192,506,012	I954T	possibly damaging	Het
Lias	T	C	5: 65,404,209	Y308H	probably damaging	Het
Lrrc27	T	A	7: 139,216,599		probably benign	Het
Lrrc8a	C	T	2: 30,256,607	H478Y	probably benign	Het
Mdh2	T	A	5: 135,784,129		probably benign	Het
Mug1	T	A	6: 121,871,381	I688K	probably benign	Het
Nrp1	A	G	8: 128,480,939	D605G	probably benign	Het
Nt5dc3	A	G	10: 86,833,999	Y486C	possibly damaging	Het
Olfr1025-ps1	T	A	2: 85,918,649	C241*	probably null	Het
Olfr1413	T	A	1: 92,573,399	V76D	possibly damaging	Het
Olfr285	A	T	15: 98,313,073	L159H	probably damaging	Het
Pam	A	T	1: 97,834,402		probably benign	Het
Pcolce	A	G	5: 137,611,288	L14P	probably damaging	Het
Pcsk6	T	C	7: 65,931,733	I254T	probably benign	Het
Pdzrn3	A	T	6: 101,151,606	C700S	probably damaging	Het
Pglyrp2	A	G	17: 32,416,002	F462S	probably damaging	Het
Pla2g16	T	A	19: 7,574,982	Y21N	possibly damaging	Het
Plekhm1	A	T	11: 103,394,952	L219Q	probably damaging	Het
Prkch	T	A	12: 73,585,244	S28T	possibly damaging	Het
Prrc2c	A	T	1: 162,679,512	S2529R	possibly damaging	Het
Prss3	G	T	6: 41,373,893	A221D	probably damaging	Het
Rbm33	T	A	5: 28,352,876		probably benign	Het
Retreg1	T	C	15: 25,968,407	L83S	probably damaging	Het
Robo2	C	T	16: 73,967,763		probably benign	Het
Slc25a47	T	A	12: 108,854,321	F84I	probably benign	Het
Sntb2	A	G	8: 107,001,688	I423V	probably benign	Het
Spg20	A	T	3: 55,124,879	D378V	possibly damaging	Het
Spint2	A	T	7: 29,259,410	F127Y	probably damaging	Het
Stag3	A	G	5: 138,311,788	T1233A	probably benign	Het
Tenm3	A	G	8: 48,349,356	I390T	probably benign	Het
Tigd2	A	G	6: 59,210,199	D17G	probably damaging	Het
Tln2	C	A	9: 67,272,654	A812S	probably benign	Het
Tmem234	A	G	4: 129,607,524	T133A	probably benign	Het
Trnp1	A	G	4: 133,498,069	F130S	possibly damaging	Het
Tsr1	A	G	11: 74,903,826	S436G	probably benign	Het
Ttn	T	C	2: 76,710,688	I33985V	probably benign	Het
Usp34	A	C	11: 23,444,184	D2278A		Het
Vav3	T	A	3: 109,657,969	F727Y	probably benign	Het
Vcl	T	C	14: 21,010,168		probably null	Het
Vps13c	T	A	9: 67,973,403	D3507E	probably damaging	Het
Vtn	A	G	11: 78,500,264		probably benign	Het
Zfp319	A	G	8: 95,328,310	S422P	possibly damaging	Het

Other mutations in Cyp2b9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Cyp2b9	APN	7	26198505	missense	probably damaging	0.99
IGL01133:Cyp2b9	APN	7	26210235	missense	probably damaging	1.00
IGL01331:Cyp2b9	APN	7	26187715	missense	probably damaging	0.99
IGL02281:Cyp2b9	APN	7	26201104	missense	probably damaging	0.99
IGL02502:Cyp2b9	APN	7	26187814	critical splice donor site	probably null
IGL02713:Cyp2b9	APN	7	26173520	missense	probably benign	0.12
IGL03032:Cyp2b9	APN	7	26198600	splice site	probably benign
IGL03307:Cyp2b9	APN	7	26199051	missense	probably benign	0.00
R0010:Cyp2b9	UTSW	7	26186753	splice site	probably benign
R0025:Cyp2b9	UTSW	7	26200813	missense	probably benign	0.09
R0040:Cyp2b9	UTSW	7	26173474	missense	possibly damaging	0.68
R0184:Cyp2b9	UTSW	7	26187007	nonsense	probably null
R0370:Cyp2b9	UTSW	7	26210106	missense	probably damaging	1.00
R1595:Cyp2b9	UTSW	7	26200907	missense	possibly damaging	0.72
R1751:Cyp2b9	UTSW	7	26186675	missense	probably benign	0.05
R1835:Cyp2b9	UTSW	7	26200783	missense	probably benign
R1879:Cyp2b9	UTSW	7	26198569	missense	probably damaging	0.99
R2256:Cyp2b9	UTSW	7	26173605	critical splice donor site	probably null
R2257:Cyp2b9	UTSW	7	26173605	critical splice donor site	probably null
R2418:Cyp2b9	UTSW	7	26186707	missense	probably benign	0.00
R3420:Cyp2b9	UTSW	7	26210103	missense	probably damaging	1.00
R4088:Cyp2b9	UTSW	7	26173456	missense	probably damaging	0.99
R4412:Cyp2b9	UTSW	7	26198443	missense	probably damaging	1.00
R4495:Cyp2b9	UTSW	7	26200755	missense	probably benign	0.00
R4615:Cyp2b9	UTSW	7	26201125	missense	probably damaging	1.00
R5375:Cyp2b9	UTSW	7	26187742	missense	probably damaging	1.00
R5426:Cyp2b9	UTSW	7	26187655	missense	probably benign
R5862:Cyp2b9	UTSW	7	26187807	missense	probably benign	0.01
R6237:Cyp2b9	UTSW	7	26173574	missense	probably benign	0.02
R6445:Cyp2b9	UTSW	7	26186987	missense	probably benign	0.13
R6992:Cyp2b9	UTSW	7	26201139	missense	probably benign	0.00
R7515:Cyp2b9	UTSW	7	26199171	missense	probably damaging	1.00
R7654:Cyp2b9	UTSW	7	26186942	missense	possibly damaging	0.72
R7816:Cyp2b9	UTSW	7	26201092	missense	probably benign	0.01
R7850:Cyp2b9	UTSW	7	26186686	nonsense	probably null
R8790:Cyp2b9	UTSW	7	26198742	intron	probably benign
R8839:Cyp2b9	UTSW	7	26200760	missense	probably damaging	0.96
R9209:Cyp2b9	UTSW	7	26173579	missense	possibly damaging	0.52
R9723:Cyp2b9	UTSW	7	26210171	nonsense	probably null
R9787:Cyp2b9	UTSW	7	26200834	missense	probably benign	0.04
Z1177:Cyp2b9	UTSW	7	26201163	missense	probably benign	0.31

Predicted Primers

PCR Primer
(F):5'- TACAGGTCACCTCCTAGGCC -3'
(R):5'- GGGTTTTGTCTGCCAGCCTC -3'

Sequencing Primer
(F):5'- GAGCTCTTCTCTGGATTCCTGAAG -3'
(R):5'- GCCAGCCTCATTCTTCTGTGG -3'

Posted On

2021-08-30