Mutagenetix > Incidental Mutation

Incidental Mutation 'R8899:Tas2r136'

679833

Institutional Source

Beutler Lab

Gene Symbol

Tas2r136

Ensembl Gene

ENSMUSG00000053217

Gene Name

taste receptor, type 2, member 136

Synonyms

Tas2r36, mt2r52

MMRRC Submission

068756-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R8899 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

132754142-132755125 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 132754323 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 268 (D268G)

Ref Sequence

ENSEMBL: ENSMUSP00000070791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065532]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000065532
AA Change: D268G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000070791
Gene: ENSMUSG00000053217
AA Change: D268G

Domain	Start	End	E-Value	Type
Pfam:TAS2R	23	317	8.4e-68	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (70/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] TAS2R44 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al., 2002 [PubMed 12584440]). For further information on TAS2Rs, see MIM 604791.[supplied by OMIM, Mar 2009]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agxt2	A	T	15: 10,378,900 (GRCm39)	N167I	probably damaging	Het
Arel1	A	G	12: 84,981,017 (GRCm39)	I330T	probably benign	Het
Ate1	T	C	7: 129,996,389 (GRCm39)	K484E	possibly damaging	Het
Atl2	T	C	17: 80,183,469 (GRCm39)	D42G	probably benign	Het
Bltp1	T	C	3: 37,042,429 (GRCm39)	I2805T	probably damaging	Het
Cd209e	C	T	8: 3,901,212 (GRCm39)	W147*	probably null	Het
Cdc40	G	A	10: 40,717,809 (GRCm39)	Q365*	probably null	Het
Cenpe	A	G	3: 134,945,644 (GRCm39)	T1053A	probably benign	Het
Colgalt1	T	C	8: 72,076,306 (GRCm39)	S586P	probably damaging	Het
Crem	A	G	18: 3,295,370 (GRCm39)	I91T	probably damaging	Het
Csrnp3	G	A	2: 65,852,987 (GRCm39)	V460I	possibly damaging	Het
Cul1	T	A	6: 47,474,246 (GRCm39)	I136N	possibly damaging	Het
Ddx19b	A	T	8: 111,737,929 (GRCm39)	I273N	probably damaging	Het
Fap	A	G	2: 62,348,817 (GRCm39)	I505T	probably damaging	Het
Fbxo44	A	T	4: 148,238,078 (GRCm39)	Y216*	probably null	Het
Fbxw10	T	A	11: 62,748,567 (GRCm39)	M398K	probably damaging	Het
Fut10	T	C	8: 31,726,514 (GRCm39)	V423A	possibly damaging	Het
Fyco1	A	C	9: 123,655,646 (GRCm39)	D1037E	probably benign	Het
Gabrg2	G	A	11: 41,867,377 (GRCm39)	R81*	probably null	Het
Gcn1	T	C	5: 115,717,220 (GRCm39)	V204A	probably benign	Het
Gnl1	T	C	17: 36,299,608 (GRCm39)	L593P	probably damaging	Het
Grm4	T	C	17: 27,653,754 (GRCm39)	Q732R	probably damaging	Het
Icam5	T	C	9: 20,948,415 (GRCm39)	V741A	possibly damaging	Het
Iqub	C	T	6: 24,505,768 (GRCm39)	E47K	probably benign	Het
Kcnk3	A	G	5: 30,779,580 (GRCm39)	K210R	probably benign	Het
Kdm2b	T	A	5: 123,125,851 (GRCm39)	R12*	probably null	Het
Kifbp	T	C	10: 62,399,282 (GRCm39)		probably benign	Het
Kndc1	C	T	7: 139,507,708 (GRCm39)	S1222F	possibly damaging	Het
Lgi1	C	T	19: 38,294,538 (GRCm39)	H413Y	probably damaging	Het
Lhx8	A	G	3: 154,033,653 (GRCm39)	Y51H	probably damaging	Het
Lim2	T	C	7: 43,083,055 (GRCm39)	I80T	probably benign	Het
Macf1	A	G	4: 123,368,852 (GRCm39)	F405L	probably benign	Het
Mefv	G	A	16: 3,528,764 (GRCm39)	T559I	probably damaging	Het
Nin	A	G	12: 70,077,710 (GRCm39)	W1739R	probably damaging	Het
Or10h1	A	T	17: 33,418,718 (GRCm39)	K232M	probably damaging	Het
Or11j4	T	C	14: 50,630,269 (GRCm39)	F19L	probably damaging	Het
Or5t16	A	G	2: 86,818,710 (GRCm39)	I270T	probably benign	Het
Or8b3b	C	T	9: 38,584,147 (GRCm39)	V198I	probably damaging	Het
Otop3	T	C	11: 115,231,886 (GRCm39)		probably null	Het
Pate9	A	T	9: 36,446,254 (GRCm39)	C53S	probably damaging	Het
Pip5kl1	A	G	2: 32,469,082 (GRCm39)	I247V	probably benign	Het
Polr2d	T	A	18: 31,922,226 (GRCm39)	M1K	probably null	Het
Prss53	T	C	7: 127,488,193 (GRCm39)	T141A	possibly damaging	Het
Rdh12	A	G	12: 79,268,802 (GRCm39)	N293S	probably benign	Het
Rnf146	G	A	10: 29,223,754 (GRCm39)	T44I	probably benign	Het
Rsbn1l	A	T	5: 21,101,865 (GRCm39)	C588S	probably damaging	Het
Septin8	G	A	11: 53,426,862 (GRCm39)	V208I	probably damaging	Het
Sgo2a	T	G	1: 58,058,822 (GRCm39)	S1134A	possibly damaging	Het
Sh3bp4	C	T	1: 89,073,297 (GRCm39)	T715I	probably benign	Het
Snrnp200	A	G	2: 127,078,517 (GRCm39)	T1758A	probably damaging	Het
Snx6	A	T	12: 54,812,423 (GRCm39)	D70E	probably benign	Het
Spag9	T	C	11: 93,983,695 (GRCm39)	S341P	probably damaging	Het
Sparcl1	T	C	5: 104,240,590 (GRCm39)	D278G	probably benign	Het
Spmap2l	T	A	5: 77,185,200 (GRCm39)		probably null	Het
Srbd1	T	A	17: 86,292,885 (GRCm39)	S895C		Het
Stxbp5l	ATTTT	ATTTTT	16: 37,036,414 (GRCm39)		probably null	Het
Tas2r143	T	A	6: 42,377,888 (GRCm39)	Y239*	probably null	Het
Tbc1d17	C	T	7: 44,492,328 (GRCm39)	G419D	probably damaging	Het
Tff2	C	T	17: 31,362,113 (GRCm39)	W68*	probably null	Het
Thop1	T	C	10: 80,916,440 (GRCm39)	C483R	probably damaging	Het
Tmem245	A	C	4: 56,903,916 (GRCm39)		probably null	Het
Tmem67	A	G	4: 12,055,038 (GRCm39)	F655S	probably damaging	Het
Trim36	T	G	18: 46,302,264 (GRCm39)	S583R	possibly damaging	Het
Ucp1	T	C	8: 84,017,216 (GRCm39)	V2A	probably benign	Het
Ugt1a6a	T	A	1: 88,066,803 (GRCm39)	M203K	probably damaging	Het
Usp1	A	G	4: 98,819,347 (GRCm39)	K270E	probably damaging	Het
Vps13c	T	C	9: 67,841,783 (GRCm39)	F1935S	probably damaging	Het
Zfp521	C	A	18: 13,979,137 (GRCm39)	L425F	probably damaging	Het
Zfp799	G	T	17: 33,039,348 (GRCm39)	P306Q	probably damaging	Het
Zfp831	A	T	2: 174,485,978 (GRCm39)	R218W	probably damaging	Het

Other mutations in Tas2r136

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00678:Tas2r136	APN	6	132,754,161 (GRCm39)	missense	probably damaging	1.00
IGL02414:Tas2r136	APN	6	132,754,494 (GRCm39)	missense	possibly damaging	0.87
IGL02662:Tas2r136	APN	6	132,754,671 (GRCm39)	missense	probably damaging	1.00
R0607:Tas2r136	UTSW	6	132,754,375 (GRCm39)	missense	probably benign	0.09
R0785:Tas2r136	UTSW	6	132,754,890 (GRCm39)	missense	probably benign
R1727:Tas2r136	UTSW	6	132,754,753 (GRCm39)	missense	possibly damaging	0.63
R2101:Tas2r136	UTSW	6	132,754,495 (GRCm39)	missense	probably benign	0.09
R2975:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R3080:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R3746:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R3749:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R3750:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R4362:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4411:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4413:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4803:Tas2r136	UTSW	6	132,754,455 (GRCm39)	missense	probably damaging	1.00
R5283:Tas2r136	UTSW	6	132,754,374 (GRCm39)	missense	probably damaging	0.99
R5343:Tas2r136	UTSW	6	132,755,043 (GRCm39)	missense	probably benign	0.00
R5738:Tas2r136	UTSW	6	132,754,707 (GRCm39)	missense	probably damaging	1.00
R5872:Tas2r136	UTSW	6	132,754,294 (GRCm39)	missense	possibly damaging	0.46
R6770:Tas2r136	UTSW	6	132,754,345 (GRCm39)	missense	probably benign	0.03
R7326:Tas2r136	UTSW	6	132,754,869 (GRCm39)	missense	possibly damaging	0.65
R7845:Tas2r136	UTSW	6	132,754,833 (GRCm39)	missense	probably benign	0.05
R7900:Tas2r136	UTSW	6	132,754,599 (GRCm39)	missense	possibly damaging	0.93
R8422:Tas2r136	UTSW	6	132,754,290 (GRCm39)	missense	probably damaging	0.96
R8783:Tas2r136	UTSW	6	132,754,612 (GRCm39)	missense	probably benign	0.08
R9126:Tas2r136	UTSW	6	132,754,584 (GRCm39)	missense	probably damaging	1.00
R9627:Tas2r136	UTSW	6	132,754,948 (GRCm39)	missense	possibly damaging	0.61
R9642:Tas2r136	UTSW	6	132,754,462 (GRCm39)	missense	probably benign	0.13
R9749:Tas2r136	UTSW	6	132,755,106 (GRCm39)	missense	probably damaging	1.00
R9765:Tas2r136	UTSW	6	132,754,813 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TTTCAGCCAGAACCTTGCTC -3'
(R):5'- CTTGACTGGGAAGACAAAACTGAC -3'

Sequencing Primer
(F):5'- GAACCTTGCTCTCACCTGAAC -3'
(R):5'- AGCTATGACTTTCACTCTAGGCAG -3'

Posted On

2021-08-31