Mutagenetix > Incidental Mutation

Incidental Mutation 'R8900:Vmn2r104'

679935

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R8900 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20041662 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 402 (I402T)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168050
AA Change: I402T

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: I402T

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430419D17Rik	A	T	7: 131,302,468		probably benign	Het
Abca9	T	C	11: 110,154,392	T372A	probably benign	Het
Adamts8	C	A	9: 30,954,634	R493S	probably benign	Het
Ankrd33b	G	A	15: 31,297,684	T309I	probably damaging	Het
Arhgap22	G	A	14: 33,271,923	W58*	probably null	Het
B4galt4	C	T	16: 38,752,042		probably benign	Het
Bcl2a1a	A	T	9: 88,957,258	I70L	probably benign	Het
Bst1	A	G	5: 43,820,600	D97G	possibly damaging	Het
Cand2	C	T	6: 115,780,933	T52M	probably benign	Het
Cd101	C	A	3: 101,018,746	R219L	probably benign	Het
Cd163l1	G	A	7: 140,228,565	W796*	probably null	Het
Cd5l	T	A	3: 87,367,575	D140E	probably benign	Het
Cebpa	A	G	7: 35,120,481	M355V	possibly damaging	Het
Cfap74	G	A	4: 155,436,730	E620K		Het
Clec4a4	A	G	6: 123,023,916	E196G	probably damaging	Het
Cpxm1	T	A	2: 130,393,440	D544V	probably damaging	Het
Cwf19l2	T	A	9: 3,447,245	D535E	probably benign	Het
Daw1	T	G	1: 83,198,177	L212R	probably benign	Het
Dnm3	T	A	1: 162,307,876	T443S	probably benign	Het
Dock9	T	C	14: 121,580,528	D1627G	probably damaging	Het
Dohh	A	G	10: 81,387,901	I263V	probably benign	Het
Dsp	G	A	13: 38,181,179	V513M	probably damaging	Het
Dus4l	G	T	12: 31,640,693	L320I	possibly damaging	Het
Edc4	A	C	8: 105,891,225	Q1139P	probably damaging	Het
Fam220a	T	A	5: 143,563,473	C213*	probably null	Het
Fgf14	A	T	14: 123,983,914	Y159*	probably null	Het
Gm45861	A	T	8: 27,529,604	D749V	unknown	Het
Hip1	T	C	5: 135,430,290	T203A	probably benign	Het
Igfals	A	G	17: 24,880,040	D35G	possibly damaging	Het
Itga4	T	A	2: 79,314,988	I716K	probably damaging	Het
Ncaph2	T	A	15: 89,369,391	I282N	probably benign	Het
Ndufb3	T	C	1: 58,595,665	Y59H	probably damaging	Het
Nxpe3	T	C	16: 55,844,660	H399R	probably damaging	Het
Nxph1	T	C	6: 9,247,601	S191P	probably damaging	Het
Olfr29-ps1	G	T	4: 43,781,432	S299R	probably benign	Het
Olfr59	T	C	11: 74,289,587	S314P	probably damaging	Het
Pcdha11	T	G	18: 37,012,746	I630S	probably damaging	Het
Pfkl	C	G	10: 78,000,781	G134A	probably damaging	Het
Phf19	A	T	2: 34,905,472	C196S	probably damaging	Het
Piezo2	T	C	18: 63,115,025	K468R	probably benign	Het
Plekhn1	T	C	4: 156,225,621	S79G	possibly damaging	Het
Pomk	A	G	8: 25,983,356	Y190H	possibly damaging	Het
Ppp1r13b	T	A	12: 111,872,344	E33D	probably damaging	Het
Raly	A	T	2: 154,863,573	I174F	probably damaging	Het
Rint1	T	C	5: 23,811,884	V549A	possibly damaging	Het
Rnf31	T	G	14: 55,596,232	C566G	probably damaging	Het
Sdc1	C	T	12: 8,790,460	T79I	possibly damaging	Het
Sema6a	T	G	18: 47,291,115	E242A	probably damaging	Het
Slc22a30	A	C	19: 8,337,976	M430R	probably damaging	Het
Sp9	C	A	2: 73,273,519	T139K	probably benign	Het
Tbkbp1	T	C	11: 97,149,501	D29G	probably benign	Het
Tcf4	T	C	18: 69,564,690		probably benign	Het
Tenm3	A	T	8: 48,236,402	I2050N	probably damaging	Het
Tiparp	T	A	3: 65,553,182	F531I	probably damaging	Het
Tmem132b	A	T	5: 125,778,820	I539F	probably damaging	Het
Ttf2	T	C	3: 100,952,640	D666G	probably damaging	Het
Vmn1r51	T	C	6: 90,129,860	S253P	probably damaging	Het
Vmn2r16	T	C	5: 109,363,753	Y609H	probably benign	Het
Vmn2r17	A	T	5: 109,427,997	M245L	probably benign	Het
Vmn2r42	C	T	7: 8,194,793	E276K	probably benign	Het
Wdr75	C	A	1: 45,799,127	N65K	probably damaging	Het
Zfhx4	T	C	3: 5,398,864	S1361P	probably damaging	Het
Zfp317	T	A	9: 19,647,412	C396*	probably null	Het
Zfp651	A	C	9: 121,767,639	K687T	probably damaging	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20042725	missense	probably benign
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20030081	missense	probably damaging	1.00
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20029475	missense	probably benign	0.01
R7514:Vmn2r104	UTSW	17	20029529	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATTGTGGACAAGACAAACTG -3'
(R):5'- GCAAAGGTTATTGACATGGAACATCTG -3'

Sequencing Primer
(F):5'- GTGGACAAGACAAACTGAATTTTAC -3'
(R):5'- CATGTTAGACTCATTCCATGGAAG -3'

Posted On

2021-08-31