Incidental Mutation 'R8906:Egfr'
ID 680128
Institutional Source Beutler Lab
Gene Symbol Egfr
Ensembl Gene ENSMUSG00000020122
Gene Name epidermal growth factor receptor
Synonyms 9030024J15Rik, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, Wa5, Errb1, Erbb
MMRRC Submission 068699-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.933) question?
Stock # R8906 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 16702203-16868158 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 16861635 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Cysteine at position 1138 (Y1138C)
Ref Sequence ENSEMBL: ENSMUSP00000020329 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020329]
AlphaFold Q01279
Predicted Effect probably damaging
Transcript: ENSMUST00000020329
AA Change: Y1138C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000020329
Gene: ENSMUSG00000020122
AA Change: Y1138C

DomainStartEndE-ValueType
low complexity region 5 25 N/A INTRINSIC
Pfam:Recep_L_domain 57 168 1.4e-32 PFAM
low complexity region 182 195 N/A INTRINSIC
FU 228 270 6.07e-4 SMART
Pfam:Recep_L_domain 361 481 1.8e-29 PFAM
FU 496 547 8.25e-6 SMART
FU 552 601 4.38e-10 SMART
FU 614 654 4.05e1 SMART
low complexity region 677 694 N/A INTRINSIC
TyrKc 714 970 2.88e-129 SMART
low complexity region 1004 1017 N/A INTRINSIC
low complexity region 1027 1048 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.5%
  • 20x: 98.4%
Validation Efficiency 100% (88/88)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
PHENOTYPE: Mutations widely affect epithelial development. Null homozygote survival is strain dependent, with defects observed in skin, eye, brain, viscera, palate, tongue and other tisses. Other mutations produce an open eyed, curly whisker phenotype, while a dominant hypermorph yields a thickened epidermis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 90 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1810055G02Rik A C 19: 3,766,686 (GRCm39) N91T possibly damaging Het
2510009E07Rik CCGGAAGGGGAGGAGCAGTGACCCAGTTTCGGA CCGGA 16: 21,472,148 (GRCm39) probably null Het
4932414N04Rik A G 2: 68,562,498 (GRCm39) D375G possibly damaging Het
Adad2 C A 8: 120,339,725 (GRCm39) P69Q probably benign Het
Adamts3 G A 5: 89,825,575 (GRCm39) T1088I probably damaging Het
Ank2 A G 3: 126,726,720 (GRCm39) V858A probably benign Het
Ankrd13a A G 5: 114,939,798 (GRCm39) N475S probably benign Het
Barhl2 G A 5: 106,603,352 (GRCm39) T269I probably benign Het
Boc G A 16: 44,323,931 (GRCm39) R157W Het
Bsn G T 9: 107,984,752 (GRCm39) P3101T unknown Het
Catsperb C A 12: 101,486,904 (GRCm39) A477E possibly damaging Het
Ccser1 T A 6: 61,787,842 (GRCm39) I220K probably benign Het
Cd200r3 A T 16: 44,778,102 (GRCm39) I242F possibly damaging Het
Cdc34b A C 11: 94,632,911 (GRCm39) D37A probably damaging Het
Chek2 A G 5: 111,013,458 (GRCm39) probably benign Het
Cngb1 A T 8: 95,989,736 (GRCm39) V792D probably damaging Het
Cops7a T C 6: 124,939,371 (GRCm39) K93E possibly damaging Het
Crispld1 T C 1: 17,820,995 (GRCm39) I345T possibly damaging Het
Dgkd A G 1: 87,869,157 (GRCm39) D1170G probably damaging Het
Dhx35 A G 2: 158,648,918 (GRCm39) T115A possibly damaging Het
Dnai1 G A 4: 41,625,125 (GRCm39) R363H probably benign Het
Dnmbp T A 19: 43,878,681 (GRCm39) Q130L probably benign Het
Elf3 A T 1: 135,182,678 (GRCm39) L329Q probably damaging Het
Enkur A G 2: 21,201,568 (GRCm39) M39T probably benign Het
Epha7 T C 4: 28,821,615 (GRCm39) I260T probably damaging Het
Fbxo28 A T 1: 182,144,634 (GRCm39) I310K probably damaging Het
Fga C A 3: 82,939,111 (GRCm39) N495K probably benign Het
Galnt6 T C 15: 100,601,247 (GRCm39) D344G probably damaging Het
Gm572 A G 4: 148,751,290 (GRCm39) Q221R probably benign Het
Gpa33 G A 1: 165,974,216 (GRCm39) A18T probably benign Het
Gpatch11 C T 17: 79,145,289 (GRCm39) T6I probably benign Het
Gpr84 T A 15: 103,217,625 (GRCm39) S151C probably damaging Het
H2-M11 T A 17: 36,859,851 (GRCm39) Y281* probably null Het
Hadh T C 3: 131,038,891 (GRCm39) N155S probably benign Het
Hoxd13 A G 2: 74,500,266 (GRCm39) Y269C Het
Iars1 T A 13: 49,882,177 (GRCm39) C1074S probably benign Het
Ibtk G T 9: 85,625,457 (GRCm39) H98N possibly damaging Het
Igsf10 C T 3: 59,233,739 (GRCm39) G1665R probably benign Het
Inpp5d A G 1: 87,625,337 (GRCm39) probably benign Het
Ipo9 A C 1: 135,321,951 (GRCm39) V593G probably damaging Het
Irx3 T C 8: 92,526,915 (GRCm39) D263G possibly damaging Het
Kif13a A G 13: 46,927,154 (GRCm39) V1179A probably benign Het
Lrrc4c T C 2: 97,460,393 (GRCm39) Y340H probably benign Het
Lysmd1 A G 3: 95,045,219 (GRCm39) D155G probably damaging Het
Mical2 T A 7: 111,980,671 (GRCm39) I105N probably damaging Het
Mob2 A T 7: 141,563,261 (GRCm39) L66Q probably damaging Het
Myh1 A G 11: 67,096,739 (GRCm39) S337G probably benign Het
Neb T C 2: 52,096,259 (GRCm39) D1002G probably benign Het
Nebl A T 2: 17,382,928 (GRCm39) N116K probably benign Het
Nkx2-6 T C 14: 69,412,623 (GRCm39) S264P probably benign Het
Nlgn3 T C X: 100,352,390 (GRCm39) V179A probably damaging Het
Nlrp4e A G 7: 23,020,556 (GRCm39) T348A possibly damaging Het
Nomo1 A T 7: 45,722,004 (GRCm39) I982F probably benign Het
Or10j5 A G 1: 172,784,186 (GRCm39) probably benign Het
Or11g24 T G 14: 50,662,291 (GRCm39) F105C probably damaging Het
Or1j19 A T 2: 36,676,621 (GRCm39) Y28F probably benign Het
Or8g22 A G 9: 38,958,077 (GRCm39) F213L possibly damaging Het
Palld A G 8: 62,003,198 (GRCm39) probably null Het
Pcdh7 A G 5: 57,879,154 (GRCm39) Y903C probably damaging Het
Pknox2 G A 9: 36,804,167 (GRCm39) T460M possibly damaging Het
Ppm1h C T 10: 122,714,451 (GRCm39) T330I probably damaging Het
Ppp2r2b C T 18: 42,821,399 (GRCm39) R253H probably damaging Het
Pramel58 G T 5: 94,831,413 (GRCm39) R140L possibly damaging Het
Prr18 G A 17: 8,560,476 (GRCm39) A211T probably benign Het
Ptgs2 A G 1: 149,979,859 (GRCm39) I321M Het
Rara G T 11: 98,860,989 (GRCm39) R159L probably damaging Het
Rasa4 T A 5: 136,133,446 (GRCm39) I635N probably benign Het
Rxfp2 A T 5: 149,989,888 (GRCm39) H423L possibly damaging Het
Scn4b A G 9: 45,059,169 (GRCm39) I147V possibly damaging Het
Scrn3 G A 2: 73,161,352 (GRCm39) V313I probably benign Het
Scrn3 C A 2: 73,161,355 (GRCm39) P314T possibly damaging Het
Sh2b1 A G 7: 126,070,292 (GRCm39) probably null Het
Slc26a10 T C 10: 127,016,459 (GRCm39) Q3R probably benign Het
Slitrk1 A G 14: 109,149,139 (GRCm39) I524T probably damaging Het
Smcp T A 3: 92,491,530 (GRCm39) N106Y unknown Het
Spata31h1 T C 10: 82,122,379 (GRCm39) T3544A probably benign Het
St8sia5 G A 18: 77,336,172 (GRCm39) V202M probably damaging Het
Stxbp5l A T 16: 37,028,526 (GRCm39) D512E probably damaging Het
Tas1r2 A G 4: 139,397,046 (GRCm39) E795G probably damaging Het
Tdrd1 T A 19: 56,831,145 (GRCm39) V320D probably damaging Het
Tmem71 T A 15: 66,404,606 (GRCm39) I261L probably benign Het
Tns2 T C 15: 102,020,039 (GRCm39) L643P probably damaging Het
Upf1 G A 8: 70,786,815 (GRCm39) Q890* probably null Het
Usp43 A T 11: 67,782,307 (GRCm39) H370Q possibly damaging Het
Vmn1r236 T A 17: 21,507,356 (GRCm39) I158N possibly damaging Het
Vmn2r98 T A 17: 19,286,532 (GRCm39) N343K probably benign Het
Vwa8 T C 14: 79,329,815 (GRCm39) S1216P probably benign Het
Yars1 A C 4: 129,090,747 (GRCm39) D97A probably damaging Het
Zer1 G A 2: 30,001,035 (GRCm39) H129Y probably benign Het
Zfat C T 15: 67,956,404 (GRCm39) D1143N possibly damaging Het
Other mutations in Egfr
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01338:Egfr APN 11 16,813,020 (GRCm39) missense probably damaging 1.00
IGL01529:Egfr APN 11 16,813,014 (GRCm39) missense probably benign
IGL01556:Egfr APN 11 16,855,382 (GRCm39) missense probably damaging 1.00
IGL02627:Egfr APN 11 16,819,346 (GRCm39) missense probably damaging 1.00
IGL02862:Egfr APN 11 16,833,562 (GRCm39) missense probably benign 0.25
IGL02945:Egfr APN 11 16,702,514 (GRCm39) missense probably damaging 1.00
IGL02994:Egfr APN 11 16,861,811 (GRCm39) missense probably damaging 1.00
IGL03395:Egfr APN 11 16,860,261 (GRCm39) splice site probably benign
set UTSW 11 16,821,881 (GRCm39) splice site probably benign
Velvet UTSW 11 16,854,399 (GRCm39) missense probably damaging 1.00
PIT1430001:Egfr UTSW 11 16,860,214 (GRCm39) missense probably benign 0.00
R0196:Egfr UTSW 11 16,861,746 (GRCm39) missense probably benign 0.02
R0513:Egfr UTSW 11 16,822,855 (GRCm39) missense probably damaging 1.00
R0567:Egfr UTSW 11 16,822,873 (GRCm39) missense probably benign 0.01
R0629:Egfr UTSW 11 16,819,333 (GRCm39) missense probably damaging 1.00
R0961:Egfr UTSW 11 16,812,964 (GRCm39) missense probably damaging 1.00
R1163:Egfr UTSW 11 16,833,546 (GRCm39) missense probably benign 0.02
R1454:Egfr UTSW 11 16,839,920 (GRCm39) missense probably benign
R1456:Egfr UTSW 11 16,813,065 (GRCm39) missense probably benign 0.00
R1503:Egfr UTSW 11 16,819,301 (GRCm39) missense possibly damaging 0.86
R1577:Egfr UTSW 11 16,819,241 (GRCm39) missense probably benign 0.04
R1595:Egfr UTSW 11 16,856,847 (GRCm39) missense probably damaging 0.99
R1699:Egfr UTSW 11 16,809,019 (GRCm39) missense probably benign 0.14
R2172:Egfr UTSW 11 16,861,562 (GRCm39) missense probably benign 0.00
R3690:Egfr UTSW 11 16,821,881 (GRCm39) splice site probably benign
R3922:Egfr UTSW 11 16,831,495 (GRCm39) missense probably damaging 1.00
R4444:Egfr UTSW 11 16,821,027 (GRCm39) missense probably benign 0.00
R4685:Egfr UTSW 11 16,808,980 (GRCm39) missense probably damaging 1.00
R4737:Egfr UTSW 11 16,819,231 (GRCm39) missense probably damaging 0.99
R4814:Egfr UTSW 11 16,819,354 (GRCm39) missense probably damaging 1.00
R4841:Egfr UTSW 11 16,861,607 (GRCm39) missense probably benign 0.05
R4842:Egfr UTSW 11 16,861,607 (GRCm39) missense probably benign 0.05
R4903:Egfr UTSW 11 16,858,949 (GRCm39) missense probably damaging 1.00
R4964:Egfr UTSW 11 16,858,949 (GRCm39) missense probably damaging 1.00
R4985:Egfr UTSW 11 16,809,029 (GRCm39) nonsense probably null
R4998:Egfr UTSW 11 16,831,493 (GRCm39) missense possibly damaging 0.58
R5001:Egfr UTSW 11 16,854,434 (GRCm39) missense probably damaging 0.98
R5304:Egfr UTSW 11 16,834,260 (GRCm39) missense probably benign
R5309:Egfr UTSW 11 16,861,703 (GRCm39) missense probably benign 0.00
R5653:Egfr UTSW 11 16,861,617 (GRCm39) missense probably benign 0.04
R5905:Egfr UTSW 11 16,861,494 (GRCm39) missense probably damaging 1.00
R6051:Egfr UTSW 11 16,833,607 (GRCm39) missense possibly damaging 0.87
R6052:Egfr UTSW 11 16,861,554 (GRCm39) missense probably benign 0.16
R6114:Egfr UTSW 11 16,854,374 (GRCm39) missense possibly damaging 0.46
R6261:Egfr UTSW 11 16,839,964 (GRCm39) missense probably benign 0.11
R6434:Egfr UTSW 11 16,819,294 (GRCm39) missense probably benign 0.25
R6475:Egfr UTSW 11 16,841,259 (GRCm39) missense probably benign
R6799:Egfr UTSW 11 16,846,952 (GRCm39) missense probably damaging 1.00
R7143:Egfr UTSW 11 16,821,627 (GRCm39) missense probably benign 0.20
R7195:Egfr UTSW 11 16,818,162 (GRCm39) missense probably damaging 1.00
R7459:Egfr UTSW 11 16,846,967 (GRCm39) missense probably damaging 1.00
R7612:Egfr UTSW 11 16,809,025 (GRCm39) missense possibly damaging 0.74
R7757:Egfr UTSW 11 16,839,966 (GRCm39) missense possibly damaging 0.64
R7763:Egfr UTSW 11 16,841,266 (GRCm39) missense probably damaging 1.00
R8315:Egfr UTSW 11 16,825,027 (GRCm39) missense probably benign 0.08
R8320:Egfr UTSW 11 16,841,251 (GRCm39) missense probably damaging 1.00
R8324:Egfr UTSW 11 16,858,885 (GRCm39) missense probably damaging 0.98
R8324:Egfr UTSW 11 16,808,971 (GRCm39) missense probably damaging 0.99
R8347:Egfr UTSW 11 16,828,174 (GRCm39) missense probably damaging 1.00
R8440:Egfr UTSW 11 16,859,831 (GRCm39) missense probably damaging 1.00
R8511:Egfr UTSW 11 16,846,949 (GRCm39) missense probably damaging 1.00
R8708:Egfr UTSW 11 16,817,300 (GRCm39) critical splice donor site probably benign
R8804:Egfr UTSW 11 16,819,339 (GRCm39) missense probably benign 0.09
R8853:Egfr UTSW 11 16,858,885 (GRCm39) missense possibly damaging 0.93
R9177:Egfr UTSW 11 16,855,410 (GRCm39) missense probably damaging 1.00
R9268:Egfr UTSW 11 16,855,410 (GRCm39) missense probably damaging 1.00
R9335:Egfr UTSW 11 16,820,991 (GRCm39) missense probably damaging 1.00
R9417:Egfr UTSW 11 16,825,067 (GRCm39) nonsense probably null
R9454:Egfr UTSW 11 16,837,155 (GRCm39) missense probably damaging 1.00
Z1177:Egfr UTSW 11 16,819,319 (GRCm39) missense probably damaging 1.00
Z1177:Egfr UTSW 11 16,812,954 (GRCm39) missense probably benign 0.05
Predicted Primers PCR Primer
(F):5'- CAGTGAGCCACGGAATTCTG -3'
(R):5'- TAGGTACTCTGCATTTTCAGCTG -3'

Sequencing Primer
(F):5'- CACGGAATTCTGCATCAGGATCTTG -3'
(R):5'- CTGTGGGGCCCTTAAATATGCC -3'
Posted On 2021-08-31