Mutagenetix > Incidental Mutation

Incidental Mutation 'R8930:Vmn1r21'

680236

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r21

Ensembl Gene

ENSMUSG00000115343

Gene Name

vomeronasal 1 receptor 21

Synonyms

V1rc28

MMRRC Submission

068774-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R8930 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

57820549-57821442 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 57820998 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 149 (T149A)

Ref Sequence

ENSEMBL: ENSMUSP00000154295 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000203310] [ENSMUST00000203488] [ENSMUST00000226191]

AlphaFold

Q8R2C6

Predicted Effect

probably benign
Transcript: ENSMUST00000203310
AA Change: T149A

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000145398
Gene: ENSMUSG00000115343
AA Change: T149A

Domain	Start	End	E-Value	Type
Pfam:V1R	27	107	1.8e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203488
AA Change: T149A

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000144978
Gene: ENSMUSG00000115343
AA Change: T149A

Domain	Start	End	E-Value	Type
Pfam:V1R	28	105	2e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226191
AA Change: T149A

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921524L21Rik	T	C	18: 6,629,693 (GRCm39)		probably null	Het
Abl2	A	G	1: 156,461,402 (GRCm39)	T435A	probably damaging	Het
Adamtsl3	C	T	7: 82,261,069 (GRCm39)	P29S		Het
Ankrd11	C	T	8: 123,622,718 (GRCm39)	R378K	probably damaging	Het
Arap1	G	A	7: 101,057,324 (GRCm39)	R1355Q	possibly damaging	Het
Ass1	T	A	2: 31,382,387 (GRCm39)	M186K	probably damaging	Het
Bckdk	A	G	7: 127,507,182 (GRCm39)	D312G	probably benign	Het
Btg3	T	A	16: 78,170,298 (GRCm39)	N3I	probably benign	Het
Cd163	T	C	6: 124,294,882 (GRCm39)	F649L	probably damaging	Het
Cdh1	ACTCGAAATGATGTGGCTC	ACTC	8: 107,392,870 (GRCm39)		probably benign	Het
Cnksr3	A	T	10: 7,110,780 (GRCm39)	V27E	probably damaging	Het
Col12a1	A	T	9: 79,580,665 (GRCm39)	S1512T	probably benign	Het
Cstf1	A	G	2: 172,217,623 (GRCm39)	T79A	probably benign	Het
Defa24	T	C	8: 22,225,373 (GRCm39)	Y88H	probably benign	Het
Dgkz	C	T	2: 91,769,915 (GRCm39)	R389Q	probably damaging	Het
Dsg3	T	A	18: 20,672,718 (GRCm39)	D796E	probably damaging	Het
Dyrk3	G	T	1: 131,057,293 (GRCm39)	D293E	probably damaging	Het
Epb42	T	C	2: 120,854,767 (GRCm39)	D606G	probably benign	Het
Erich5	G	A	15: 34,453,844 (GRCm39)	G18S	probably benign	Het
Fam8a1	C	T	13: 46,827,868 (GRCm39)	T352M	probably benign	Het
Fastkd3	T	A	13: 68,731,835 (GRCm39)	L52Q	probably benign	Het
Fat3	G	A	9: 15,910,819 (GRCm39)	H1728Y	probably benign	Het
Fgl1	A	G	8: 41,662,868 (GRCm39)	V39A	probably benign	Het
Gm7298	T	C	6: 121,742,030 (GRCm39)	V484A	probably benign	Het
Gpr75	A	G	11: 30,842,571 (GRCm39)	Q492R	possibly damaging	Het
Hoxc8	A	G	15: 102,899,318 (GRCm39)	H36R	possibly damaging	Het
Insyn2a	A	G	7: 134,500,881 (GRCm39)	I408T	probably damaging	Het
Insyn2b	T	C	11: 34,352,707 (GRCm39)	S250P	probably benign	Het
Isy1	T	C	6: 87,798,513 (GRCm39)	I214V	probably damaging	Het
Kdm5b	C	T	1: 134,544,010 (GRCm39)	P853L	probably damaging	Het
Man2b1	A	G	8: 85,822,022 (GRCm39)	T746A	probably damaging	Het
Mast3	A	T	8: 71,234,377 (GRCm39)		probably benign	Het
Mki67	A	G	7: 135,300,628 (GRCm39)	S1469P	possibly damaging	Het
Mrps30	C	T	13: 118,523,695 (GRCm39)	A26T	probably benign	Het
Mup10	T	A	4: 60,536,708 (GRCm39)	T92S	possibly damaging	Het
Otud7a	A	G	7: 63,407,239 (GRCm39)	D514G	possibly damaging	Het
Pacs1	T	A	19: 5,185,030 (GRCm39)	S932C	probably damaging	Het
Pbx2	C	T	17: 34,813,563 (GRCm39)	R188C	probably damaging	Het
Pclo	C	T	5: 14,762,989 (GRCm39)	P536S	possibly damaging	Het
Pdxdc1	A	T	16: 13,672,269 (GRCm39)	I377N	probably damaging	Het
Peg10	GAT	GATCAT	6: 4,756,449 (GRCm39)		probably benign	Het
Phip	T	C	9: 82,789,041 (GRCm39)	K758E	possibly damaging	Het
Pramel34	T	G	5: 93,785,944 (GRCm39)	D112A	probably benign	Het
Ptprm	C	T	17: 67,263,846 (GRCm39)	R467H	probably benign	Het
Scnn1b	C	T	7: 121,502,067 (GRCm39)	R242C	probably damaging	Het
Seh1l	T	C	18: 67,908,134 (GRCm39)	S19P	possibly damaging	Het
Slc28a1	T	A	7: 80,817,715 (GRCm39)	V528D	probably benign	Het
Slc6a17	G	T	3: 107,379,507 (GRCm39)	Q554K	probably benign	Het
Spag16	G	A	1: 70,338,928 (GRCm39)		probably null	Het
Spata31d1e	C	T	13: 59,890,015 (GRCm39)	E184K	possibly damaging	Het
Srfbp1	T	A	18: 52,623,117 (GRCm39)	N377K	possibly damaging	Het
Stap2	A	G	17: 56,304,895 (GRCm39)	S296P	probably benign	Het
Tnr	A	T	1: 159,740,359 (GRCm39)	I1178F	probably damaging	Het
Tomt	C	T	7: 101,550,350 (GRCm39)	A139T	probably damaging	Het
Trak2	T	C	1: 58,974,967 (GRCm39)	Q75R	probably benign	Het
Tulp1	T	C	17: 28,583,468 (GRCm39)	K58E	probably benign	Het
Utp6	A	G	11: 79,834,055 (GRCm39)		probably null	Het
Vmn1r238	C	T	18: 3,123,127 (GRCm39)	V96M	probably benign	Het
Vmn1r9	T	A	6: 57,048,666 (GRCm39)	I247N	probably damaging	Het
Vps33b	T	C	7: 79,932,241 (GRCm39)	C138R	possibly damaging	Het
Wnk1	A	T	6: 119,940,226 (GRCm39)	V837E	probably damaging	Het
Xirp2	C	T	2: 67,312,707 (GRCm39)	H59Y	possibly damaging	Het
Zbtb38	G	T	9: 96,568,434 (GRCm39)	D883E	probably benign	Het
Zbtb7a	C	A	10: 80,980,368 (GRCm39)	D187E	probably benign	Het
Zfp511	T	C	7: 139,617,442 (GRCm39)	V148A	probably damaging	Het
Zfp52	A	G	17: 21,780,692 (GRCm39)	D180G	possibly damaging	Het

Other mutations in Vmn1r21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Vmn1r21	APN	6	57,821,049 (GRCm39)	missense	probably benign	0.05
IGL01366:Vmn1r21	APN	6	57,820,799 (GRCm39)	missense	probably benign	0.01
IGL01660:Vmn1r21	APN	6	57,821,222 (GRCm39)	missense	probably damaging	0.99
IGL02864:Vmn1r21	APN	6	57,820,661 (GRCm39)	missense	probably benign	0.13
IGL02961:Vmn1r21	APN	6	57,820,974 (GRCm39)	missense	probably benign	0.01
IGL03170:Vmn1r21	APN	6	57,820,847 (GRCm39)	missense	probably damaging	0.99
PIT4453001:Vmn1r21	UTSW	6	57,821,307 (GRCm39)	missense	probably benign	0.04
R1800:Vmn1r21	UTSW	6	57,820,799 (GRCm39)	missense	probably benign	0.01
R1928:Vmn1r21	UTSW	6	57,821,077 (GRCm39)	nonsense	probably null
R3407:Vmn1r21	UTSW	6	57,820,877 (GRCm39)	missense	probably damaging	1.00
R5566:Vmn1r21	UTSW	6	57,821,079 (GRCm39)	missense	probably benign	0.06
R6012:Vmn1r21	UTSW	6	57,820,891 (GRCm39)	missense	probably damaging	1.00
R6293:Vmn1r21	UTSW	6	57,821,255 (GRCm39)	missense	probably benign	0.19
R6473:Vmn1r21	UTSW	6	57,820,583 (GRCm39)	missense	probably damaging	0.99
R7128:Vmn1r21	UTSW	6	57,820,936 (GRCm39)	missense	probably damaging	0.97
R7489:Vmn1r21	UTSW	6	57,820,877 (GRCm39)	missense	probably damaging	1.00
R7559:Vmn1r21	UTSW	6	57,821,227 (GRCm39)	missense	probably damaging	0.99
R8002:Vmn1r21	UTSW	6	57,821,199 (GRCm39)	missense	probably benign	0.00
R8218:Vmn1r21	UTSW	6	57,820,910 (GRCm39)	missense	noncoding transcript
R8467:Vmn1r21	UTSW	6	57,821,441 (GRCm39)	start codon destroyed	probably null	1.00
R8922:Vmn1r21	UTSW	6	57,820,829 (GRCm39)	missense	probably damaging	1.00
R8932:Vmn1r21	UTSW	6	57,820,998 (GRCm39)	missense	probably benign	0.01
R8961:Vmn1r21	UTSW	6	57,820,829 (GRCm39)	missense	probably damaging	1.00
R9154:Vmn1r21	UTSW	6	57,821,348 (GRCm39)	missense	probably benign	0.36
Z1176:Vmn1r21	UTSW	6	57,820,563 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCAAGATTATCTGGGTGGCC -3'
(R):5'- GAGGCCTCTCTATCTGCATCAC -3'

Sequencing Primer
(F):5'- CTATGAAGATGCTTGCATTGCC -3'
(R):5'- ACCTGCCTCCTGAGTGTG -3'

Posted On

2021-08-31