Incidental Mutation 'R8932:Gm7298'
ID 680362
Institutional Source Beutler Lab
Gene Symbol Gm7298
Ensembl Gene ENSMUSG00000108022
Gene Name predicted gene 7298
Synonyms
MMRRC Submission 068776-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.235) question?
Stock # R8932 (G1)
Quality Score 225.009
Status Not validated
Chromosome 6
Chromosomal Location 121711454-121761598 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 121742030 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 484 (V484A)
Ref Sequence ENSEMBL: ENSMUSP00000145242 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000204124]
AlphaFold A0A0N4SVU1
Predicted Effect probably benign
Transcript: ENSMUST00000204124
AA Change: V484A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000145242
Gene: ENSMUSG00000108022
AA Change: V484A

DomainStartEndE-ValueType
signal peptide 1 24 N/A INTRINSIC
Pfam:A2M_N 128 221 4e-18 PFAM
A2M_N_2 449 599 1e-45 SMART
A2M 740 830 2.1e-39 SMART
Pfam:Thiol-ester_cl 963 992 1.9e-15 PFAM
Pfam:A2M_comp 1012 1268 1.6e-90 PFAM
A2M_recep 1378 1465 4.3e-42 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.9%
  • 20x: 99.6%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921524L21Rik T C 18: 6,629,693 (GRCm39) probably null Het
Abl2 A G 1: 156,461,402 (GRCm39) T435A probably damaging Het
Adamtsl3 C T 7: 82,261,069 (GRCm39) P29S Het
Ass1 T A 2: 31,382,387 (GRCm39) M186K probably damaging Het
Bckdk A G 7: 127,507,182 (GRCm39) D312G probably benign Het
Btg3 T A 16: 78,170,298 (GRCm39) N3I probably benign Het
Cd163 T C 6: 124,294,882 (GRCm39) F649L probably damaging Het
Cdh1 ACTCGAAATGATGTGGCTC ACTC 8: 107,392,870 (GRCm39) probably benign Het
Cnksr3 A T 10: 7,110,780 (GRCm39) V27E probably damaging Het
Col12a1 A T 9: 79,580,665 (GRCm39) S1512T probably benign Het
Cstf1 A G 2: 172,217,623 (GRCm39) T79A probably benign Het
Defa24 T C 8: 22,225,373 (GRCm39) Y88H probably benign Het
Dgkz C T 2: 91,769,915 (GRCm39) R389Q probably damaging Het
Dsg3 T A 18: 20,672,718 (GRCm39) D796E probably damaging Het
Dyrk3 G T 1: 131,057,293 (GRCm39) D293E probably damaging Het
Epb42 T C 2: 120,854,767 (GRCm39) D606G probably benign Het
Erich5 G A 15: 34,453,844 (GRCm39) G18S probably benign Het
Fam8a1 C T 13: 46,827,868 (GRCm39) T352M probably benign Het
Fastkd3 T A 13: 68,731,835 (GRCm39) L52Q probably benign Het
Fat3 G A 9: 15,910,819 (GRCm39) H1728Y probably benign Het
Fgl1 A G 8: 41,662,868 (GRCm39) V39A probably benign Het
Gpr75 A G 11: 30,842,571 (GRCm39) Q492R possibly damaging Het
Hoxc8 A G 15: 102,899,318 (GRCm39) H36R possibly damaging Het
Insyn2a A G 7: 134,500,881 (GRCm39) I408T probably damaging Het
Insyn2b T C 11: 34,352,707 (GRCm39) S250P probably benign Het
Isy1 T C 6: 87,798,513 (GRCm39) I214V probably damaging Het
Kdm5b C T 1: 134,544,010 (GRCm39) P853L probably damaging Het
Man2b1 A G 8: 85,822,022 (GRCm39) T746A probably damaging Het
Mki67 A G 7: 135,300,628 (GRCm39) S1469P possibly damaging Het
Mrps30 C T 13: 118,523,695 (GRCm39) A26T probably benign Het
Mup10 T A 4: 60,536,708 (GRCm39) T92S possibly damaging Het
Otud7a A G 7: 63,407,239 (GRCm39) D514G possibly damaging Het
Pacs1 T A 19: 5,185,030 (GRCm39) S932C probably damaging Het
Pbx2 C T 17: 34,813,563 (GRCm39) R188C probably damaging Het
Pclo C T 5: 14,762,989 (GRCm39) P536S possibly damaging Het
Pdxdc1 A T 16: 13,672,269 (GRCm39) I377N probably damaging Het
Phip T C 9: 82,789,041 (GRCm39) K758E possibly damaging Het
Pramel34 T G 5: 93,785,944 (GRCm39) D112A probably benign Het
Ptprm C T 17: 67,263,846 (GRCm39) R467H probably benign Het
Rbm12b1 C T 4: 12,145,689 (GRCm39) R554C probably benign Het
Scnn1b C T 7: 121,502,067 (GRCm39) R242C probably damaging Het
Seh1l T C 18: 67,908,134 (GRCm39) S19P possibly damaging Het
Slc28a1 T A 7: 80,817,715 (GRCm39) V528D probably benign Het
Slc6a17 G T 3: 107,379,507 (GRCm39) Q554K probably benign Het
Spag16 G A 1: 70,338,928 (GRCm39) probably null Het
Spata31d1e C T 13: 59,890,015 (GRCm39) E184K possibly damaging Het
Srfbp1 T A 18: 52,623,117 (GRCm39) N377K possibly damaging Het
Stap2 A G 17: 56,304,895 (GRCm39) S296P probably benign Het
Tnr A T 1: 159,740,359 (GRCm39) I1178F probably damaging Het
Tomt C T 7: 101,550,350 (GRCm39) A139T probably damaging Het
Trak2 T C 1: 58,974,967 (GRCm39) Q75R probably benign Het
Tulp1 T C 17: 28,583,468 (GRCm39) K58E probably benign Het
Utp6 A G 11: 79,834,055 (GRCm39) probably null Het
Vmn1r21 T C 6: 57,820,998 (GRCm39) T149A probably benign Het
Vmn1r238 C T 18: 3,123,127 (GRCm39) V96M probably benign Het
Vmn1r9 T A 6: 57,048,666 (GRCm39) I247N probably damaging Het
Vps33b T C 7: 79,932,241 (GRCm39) C138R possibly damaging Het
Wnk1 A T 6: 119,940,226 (GRCm39) V837E probably damaging Het
Xirp2 C T 2: 67,312,707 (GRCm39) H59Y possibly damaging Het
Zbtb38 G T 9: 96,568,434 (GRCm39) D883E probably benign Het
Zbtb7a C A 10: 80,980,368 (GRCm39) D187E probably benign Het
Zfp511 T C 7: 139,617,442 (GRCm39) V148A probably damaging Het
Zfp52 A G 17: 21,780,692 (GRCm39) D180G possibly damaging Het
Other mutations in Gm7298
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0030:Gm7298 UTSW 6 121,751,009 (GRCm39) missense probably benign
R4978:Gm7298 UTSW 6 121,710,076 (GRCm39) critical splice donor site probably null
R4980:Gm7298 UTSW 6 121,736,198 (GRCm39) splice site probably null
R6000:Gm7298 UTSW 6 121,742,038 (GRCm39) missense possibly damaging 0.91
R6160:Gm7298 UTSW 6 121,741,886 (GRCm39) missense probably benign 0.28
R6180:Gm7298 UTSW 6 121,737,782 (GRCm39) missense probably benign 0.01
R6243:Gm7298 UTSW 6 121,756,096 (GRCm39) missense possibly damaging 0.88
R6266:Gm7298 UTSW 6 121,759,663 (GRCm39) missense probably damaging 1.00
R6268:Gm7298 UTSW 6 121,756,032 (GRCm39) missense possibly damaging 0.83
R6363:Gm7298 UTSW 6 121,765,565 (GRCm39) missense probably damaging 1.00
R6364:Gm7298 UTSW 6 121,756,402 (GRCm39) missense possibly damaging 0.90
R6527:Gm7298 UTSW 6 121,746,669 (GRCm39) missense probably benign 0.01
R6538:Gm7298 UTSW 6 121,753,132 (GRCm39) missense probably damaging 0.98
R6801:Gm7298 UTSW 6 121,752,768 (GRCm39) missense probably benign 0.03
R6884:Gm7298 UTSW 6 121,737,480 (GRCm39) missense possibly damaging 0.74
R6935:Gm7298 UTSW 6 121,744,653 (GRCm39) missense probably benign 0.02
R7051:Gm7298 UTSW 6 121,751,993 (GRCm39) critical splice donor site probably null
R7144:Gm7298 UTSW 6 121,738,546 (GRCm39) missense probably damaging 0.99
R7178:Gm7298 UTSW 6 121,762,855 (GRCm39) missense probably damaging 0.98
R7398:Gm7298 UTSW 6 121,758,912 (GRCm39) missense probably benign 0.02
R7706:Gm7298 UTSW 6 121,712,570 (GRCm39) missense probably damaging 0.96
R7793:Gm7298 UTSW 6 121,737,563 (GRCm39) critical splice donor site probably null
R7829:Gm7298 UTSW 6 121,742,297 (GRCm39) missense probably damaging 1.00
R7877:Gm7298 UTSW 6 121,759,741 (GRCm39) nonsense probably null
R8010:Gm7298 UTSW 6 121,712,542 (GRCm39) missense probably benign
R8167:Gm7298 UTSW 6 121,761,414 (GRCm39) nonsense probably null
R8188:Gm7298 UTSW 6 121,763,537 (GRCm39) critical splice acceptor site probably null
R8248:Gm7298 UTSW 6 121,764,402 (GRCm39) missense probably benign 0.02
R8669:Gm7298 UTSW 6 121,742,002 (GRCm39) missense probably benign
R8806:Gm7298 UTSW 6 121,761,641 (GRCm39) synonymous silent
R8867:Gm7298 UTSW 6 121,748,788 (GRCm39) missense probably benign
R8907:Gm7298 UTSW 6 121,741,817 (GRCm39) missense probably benign 0.10
R8930:Gm7298 UTSW 6 121,742,030 (GRCm39) missense probably benign 0.01
R8947:Gm7298 UTSW 6 121,757,553 (GRCm39) missense possibly damaging 0.62
R9016:Gm7298 UTSW 6 121,758,800 (GRCm39) missense possibly damaging 0.96
R9040:Gm7298 UTSW 6 121,764,438 (GRCm39) missense probably benign 0.20
R9069:Gm7298 UTSW 6 121,761,393 (GRCm39) missense probably benign
R9154:Gm7298 UTSW 6 121,756,436 (GRCm39) missense probably damaging 1.00
R9273:Gm7298 UTSW 6 121,756,604 (GRCm39) intron probably benign
R9371:Gm7298 UTSW 6 121,744,541 (GRCm39) missense probably benign 0.02
R9372:Gm7298 UTSW 6 121,748,746 (GRCm39) missense probably benign 0.21
R9490:Gm7298 UTSW 6 121,751,083 (GRCm39) missense probably benign 0.00
R9649:Gm7298 UTSW 6 121,764,491 (GRCm39) missense probably damaging 1.00
Z1176:Gm7298 UTSW 6 121,741,834 (GRCm39) missense possibly damaging 0.48
Z1176:Gm7298 UTSW 6 121,741,829 (GRCm39) missense probably benign 0.03
Predicted Primers PCR Primer
(F):5'- TCCCATTTCTATTGCATGGAGG -3'
(R):5'- TGGGCCATGACCTAAAATGG -3'

Sequencing Primer
(F):5'- TCTATTGCATGGAGGAAAGATATGC -3'
(R):5'- CAAGACTTTGTTCTAACTGTGCTAG -3'
Posted On 2021-08-31