Mutagenetix > Incidental Mutation

Incidental Mutation 'R8933:Wnk1'

680439

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8933 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 120036998 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 212 (V212A)

Ref Sequence

ENSEMBL: ENSMUSP00000086019 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

probably damaging
Transcript: ENSMUST00000060043
AA Change: V212A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: V212A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000088644
AA Change: V212A

PolyPhen 2 Score 0.738 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: V212A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000088646
AA Change: V212A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: V212A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000177761
AA Change: V212A

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: V212A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000203030
AA Change: V212A

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: V212A

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (86/86)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 86 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 122,128,137	I1114N	probably damaging	Het
Ache	C	A	5: 137,290,187	R52S	possibly damaging	Het
AI182371	A	T	2: 35,085,702		probably null	Het
Ankrd17	T	C	5: 90,258,466	S1453G	probably damaging	Het
Arvcf	A	G	16: 18,400,095	N508S	probably damaging	Het
Aurkc	A	C	7: 7,002,797	D136A	possibly damaging	Het
Bfsp2	A	G	9: 103,448,649	M265T	probably benign	Het
Bmpr1b	T	C	3: 141,856,608	T273A	probably damaging	Het
Cacnb1	T	C	11: 98,005,752	K406R	probably damaging	Het
Calcoco2	C	T	11: 96,107,426		probably benign	Het
Capza1	T	C	3: 104,840,893		probably null	Het
Ccdc63	T	A	5: 122,113,202	I382F	probably damaging	Het
Cep350	A	T	1: 155,863,415	N2227K	probably benign	Het
Chia1	T	A	3: 106,129,017	Y304*	probably null	Het
Col5a2	G	A	1: 45,421,963	P258L		Het
Cyp21a1	A	G	17: 34,804,311	L30P	probably damaging	Het
Dennd4b	T	A	3: 90,279,216	H1351Q	probably benign	Het
Dnah9	T	A	11: 65,855,252	I4012F	possibly damaging	Het
Drd1	A	G	13: 54,053,271	I301T	possibly damaging	Het
Dzip1	T	A	14: 118,906,914	H369L	probably damaging	Het
Fgfrl1	T	A	5: 108,703,391	M58K	probably damaging	Het
Ftsj3	G	T	11: 106,250,834	D529E	probably benign	Het
Fut9	A	T	4: 25,619,861	W318R	probably damaging	Het
Gabrg3	A	G	7: 56,984,958	I159T	probably damaging	Het
Gabrr1	A	T	4: 33,146,972	D53V	probably benign	Het
Ggps1	A	G	13: 14,054,343	V85A	probably benign	Het
Gm5141	C	T	13: 62,777,040	W18*	probably null	Het
Grik5	T	A	7: 25,023,318	T518S	probably benign	Het
Hapln3	A	T	7: 79,117,630		probably benign	Het
Hbs1l	C	T	10: 21,367,685	Q646*	probably null	Het
Hmox1	T	C	8: 75,097,016	I104T	probably benign	Het
Ifit1bl1	T	C	19: 34,594,013	Y348C	probably damaging	Het
Igf2r	A	T	17: 12,701,244	S1403T	probably damaging	Het
Igf2r	G	A	17: 12,704,637	T1186M	probably damaging	Het
Igfbp1	T	C	11: 7,198,333		probably null	Het
Ikbip	C	A	10: 91,083,230	A35E	probably benign	Het
Isca1	C	T	13: 59,769,683	A8T	probably damaging	Het
Itgb2	T	C	10: 77,565,188	L754P	probably damaging	Het
Itgb6	C	T	2: 60,627,903	C502Y	probably damaging	Het
Kdm3a	A	G	6: 71,600,108	V741A	probably benign	Het
Kdsr	T	C	1: 106,753,219	D83G	possibly damaging	Het
Kiz	T	A	2: 146,942,117	N523K		Het
Kmt2a	T	C	9: 44,822,505		probably benign	Het
Krt25	T	C	11: 99,321,238	E191G	probably benign	Het
Lipn	A	G	19: 34,069,480	I61V	probably damaging	Het
Lmbr1l	T	A	15: 98,909,269		probably null	Het
Lrrc4c	A	G	2: 97,629,481	K151E	probably benign	Het
Mab21l3	T	C	3: 101,823,458	Q155R	probably benign	Het
Mtfr2	G	A	10: 20,357,528	R281H	possibly damaging	Het
Muc5ac	A	G	7: 141,789,756	Y35C	possibly damaging	Het
Myadm	C	T	7: 3,296,917	T65I	probably benign	Het
Nap1l1	T	C	10: 111,492,849	V213A	probably benign	Het
Nav2	A	G	7: 49,461,957	D737G	probably damaging	Het
Ndst4	T	A	3: 125,611,506	V470D	probably damaging	Het
Nek5	T	A	8: 22,111,210	Y165F	probably damaging	Het
Nek5	A	T	8: 22,120,843	V48E	probably damaging	Het
Nr6a1	T	C	2: 38,760,388	I77V	probably damaging	Het
Nvl	A	T	1: 181,139,073	D93E	probably benign	Het
Olfr450	A	G	6: 42,818,016	T182A	probably benign	Het
Olfr552	A	T	7: 102,604,430	L25F	probably damaging	Het
Olfr656	A	T	7: 104,617,666	T4S	probably benign	Het
Olfr678	A	G	7: 105,069,392		probably benign	Het
Olfr780	A	T	10: 129,322,390	I256F	probably damaging	Het
Olfr96	C	A	17: 37,225,455	T110K	possibly damaging	Het
Pla2g4c	G	A	7: 13,339,702	V225I	probably benign	Het
Plppr4	T	A	3: 117,323,041	N331I	probably damaging	Het
Ptpn13	A	G	5: 103,579,805	R2051G	probably benign	Het
Rbm45	T	C	2: 76,378,724	S346P	probably damaging	Het
Samd14	A	C	11: 95,021,201	D168A	probably damaging	Het
Slc15a1	C	T	14: 121,486,679	G172R	probably benign	Het
Slco3a1	G	T	7: 74,284,500	Y641*	probably null	Het
Srcap	G	A	7: 127,552,394	R2027H	probably damaging	Het
Stra8	A	T	6: 34,927,689		probably benign	Het
Syt11	T	C	3: 88,747,704	Y430C	probably damaging	Het
Tanc1	T	G	2: 59,785,456	V269G	possibly damaging	Het
Tead1	A	G	7: 112,898,611	N342S	probably benign	Het
Tenm3	C	T	8: 48,279,060	A1254T	possibly damaging	Het
Txlnb	A	T	10: 17,806,798	N156I	probably damaging	Het
Ufl1	A	T	4: 25,262,258	S409R	possibly damaging	Het
Usp17la	A	G	7: 104,861,100	H304R	probably benign	Het
Vmn1r203	T	A	13: 22,524,521	H157Q	possibly damaging	Het
Vmn1r37	C	T	6: 66,732,247	R249*	probably null	Het
Vmn2r96	A	G	17: 18,583,979	Q497R	probably benign	Het
Vps39	A	G	2: 120,338,585	S292P	probably benign	Het
Xbp1	T	C	11: 5,524,741	V161A	probably benign	Het
Zfp113	T	C	5: 138,144,830	Q386R	probably damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119960708	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119937411	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119948397	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119963485	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119949078	unclassified	probably benign
IGL02268:Wnk1	APN	6	119937373	nonsense	probably null
IGL02348:Wnk1	APN	6	119963328	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119963454	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119937862	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119926633	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119954187	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119953895	missense	probably benign	0.41
Actor	UTSW	6	119970978	missense	probably damaging	1.00
Bad	UTSW	6	119965678	intron	probably benign
Blink	UTSW	6	120002273	nonsense	probably null
Knock	UTSW	6	119943878	splice site	probably null
Narrow	UTSW	6	119992408	missense	probably damaging	1.00
nictitate	UTSW	6	120002280	missense	possibly damaging	0.88
opportunity	UTSW	6	119951068	missense	probably damaging	1.00
path	UTSW	6	120037149	missense	probably damaging	0.99
Stormy	UTSW	6	119992447	missense	probably damaging	1.00
tear	UTSW	6	119963470	missense	probably damaging	0.98
Tic	UTSW	6	119950083	missense	probably damaging	1.00
Unforgiving	UTSW	6	119948282	missense	probably damaging	1.00
Window	UTSW	6	119992453	missense	probably damaging	1.00
Woke	UTSW	6	119962955	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119944799	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119963367	nonsense	probably null
R0044:Wnk1	UTSW	6	120037149	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119952733	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119928163	intron	probably benign
R0453:Wnk1	UTSW	6	119963151	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119969332	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119962803	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119926564	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119951992	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119926683	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119948505	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119952006	splice site	probably benign
R1188:Wnk1	UTSW	6	119948709	nonsense	probably null
R1245:Wnk1	UTSW	6	119948457	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119950684	splice site	probably benign
R1869:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119952923	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119934382	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119937578	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119928157	splice site	probably null
R2144:Wnk1	UTSW	6	119948988	unclassified	probably benign
R2186:Wnk1	UTSW	6	119948567	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119963640	splice site	probably null
R2338:Wnk1	UTSW	6	119969534	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119936367	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119992453	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119969354	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119949051	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119951068	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119948126	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119949261	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119953895	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119926426	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119951911	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119965744	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119963470	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119952771	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119962438	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119949377	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119965735	intron	probably benign
R5002:Wnk1	UTSW	6	119937963	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119965735	intron	probably benign
R5152:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120037188	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119968523	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119948891	unclassified	probably benign
R5600:Wnk1	UTSW	6	119949358	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119992408	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120037601	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119972997	intron	probably benign
R6128:Wnk1	UTSW	6	119963786	splice site	probably null
R6237:Wnk1	UTSW	6	119952767	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119948585	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119962955	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119948282	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119948781	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119965678	intron	probably benign
R7024:Wnk1	UTSW	6	119965726	intron	probably benign
R7072:Wnk1	UTSW	6	119937861	missense	unknown
R7087:Wnk1	UTSW	6	120037530	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119948307	missense	unknown
R7134:Wnk1	UTSW	6	119926428	missense	unknown
R7137:Wnk1	UTSW	6	120038212	unclassified	probably benign
R7142:Wnk1	UTSW	6	119949279	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119970978	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119943878	splice site	probably null
R7218:Wnk1	UTSW	6	120002273	nonsense	probably null
R7498:Wnk1	UTSW	6	119927196	missense	unknown
R7599:Wnk1	UTSW	6	119929828	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119932738	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119949176	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120037448	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119932714	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119953833	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119963493	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119929999	splice site	probably null
R8359:Wnk1	UTSW	6	119992447	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119934427	missense	unknown
R8519:Wnk1	UTSW	6	119950083	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R9005:Wnk1	UTSW	6	119962432	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119948670	missense	unknown
R9148:Wnk1	UTSW	6	119948670	missense	unknown
R9354:Wnk1	UTSW	6	119965699	missense	unknown
R9379:Wnk1	UTSW	6	119951717	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120037032	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119948205	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CTCGACAAACTGGCTTAACAG -3'
(R):5'- TGGGGAACAAGCCGTTGTAG -3'

Sequencing Primer
(F):5'- CGACTTCCACAGTGGTTT -3'
(R):5'- ACGACCGTCCCCAGCAG -3'

Posted On

2021-08-31