Mutagenetix > Incidental Mutation

Incidental Mutation 'R8948:Map2'

681429

Institutional Source

Beutler Lab

Gene Symbol

Map2

Ensembl Gene

ENSMUSG00000015222

Gene Name

microtubule-associated protein 2

Synonyms

G1-397-34, MAP-2, Mtap2, repro4

MMRRC Submission

068786-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.751) question?

Stock #

R8948 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66214432-66481742 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 66419684 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Leucine at position 5 (R5L)

Ref Sequence

ENSEMBL: ENSMUSP00000109651 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024639] [ENSMUST00000077355] [ENSMUST00000114012] [ENSMUST00000114013] [ENSMUST00000114015] [ENSMUST00000114017] [ENSMUST00000114018] [ENSMUST00000123647] [ENSMUST00000156636] [ENSMUST00000173855]

AlphaFold

P20357

Predicted Effect

probably damaging
Transcript: ENSMUST00000024639
AA Change: R5L

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000024639
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000077355
AA Change: R5L

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000076577
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114012
AA Change: R5L

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000109645
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	133	140	N/A	INTRINSIC
low complexity region	181	195	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	228	250	N/A	INTRINSIC
Pfam:Tubulin-binding	299	330	2.1e-18	PFAM
Pfam:Tubulin-binding	331	361	9.1e-20	PFAM
Pfam:Tubulin-binding	362	393	1.7e-17	PFAM
low complexity region	421	434	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114013
AA Change: R5L

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000109646
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	1.2e-5	PFAM
low complexity region	120	141	N/A	INTRINSIC
Pfam:MAP2_projctn	376	1510	N/A	PFAM
low complexity region	1543	1557	N/A	INTRINSIC
low complexity region	1567	1583	N/A	INTRINSIC
low complexity region	1590	1612	N/A	INTRINSIC
Pfam:Tubulin-binding	1662	1692	1.7e-13	PFAM
Pfam:Tubulin-binding	1693	1723	5.8e-18	PFAM
Pfam:Tubulin-binding	1724	1755	5.9e-18	PFAM
low complexity region	1783	1796	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114015
AA Change: R5L

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000109648
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114017
AA Change: R5L

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000109650
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114018
AA Change: R5L

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000109651
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000123647
AA Change: R5L

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000119341
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	3.8e-5	PFAM
low complexity region	120	135	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000141148

SMART Domains

Protein: ENSMUSP00000117996
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	23	40	3.2e-6	PFAM
low complexity region	70	77	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000156636
AA Change: R5L

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000173855
AA Change: R5L

PolyPhen 2 Score 0.896 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000134471
Gene: ENSMUSG00000015222
AA Change: R5L

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	142	163	N/A	INTRINSIC
Pfam:MAP2_projctn	458	565	1.1e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered contextual memory. Mice homozygous for another knock-out allele display decreased body weight, altered microtubule density and organization in Purkinje cell dendrites, and reduced dendritic length inhippocampal neurons. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	T	C	16: 8,418,805 (GRCm39)	I102T	possibly damaging	Het
Abca9	T	C	11: 110,054,206 (GRCm39)		probably null	Het
Ago3	T	C	4: 126,244,115 (GRCm39)		probably null	Het
Akap1	G	T	11: 88,735,099 (GRCm39)	A75E	probably damaging	Het
Atad2b	C	A	12: 5,041,012 (GRCm39)	A227E	possibly damaging	Het
Bmpr1a	G	T	14: 34,163,148 (GRCm39)	H81N	possibly damaging	Het
Camkv	C	T	9: 107,824,296 (GRCm39)	T236I	probably damaging	Het
Ccdc30	T	C	4: 119,181,358 (GRCm39)	E601G	probably benign	Het
Ccin	G	A	4: 43,984,064 (GRCm39)	R157H	probably damaging	Het
Cdkn3	C	T	14: 47,004,780 (GRCm39)	P114L	probably damaging	Het
Col6a2	C	T	10: 76,446,527 (GRCm39)	G352D	probably damaging	Het
Csf2rb	A	T	15: 78,232,520 (GRCm39)	D609V	probably benign	Het
Ctc1	T	G	11: 68,917,175 (GRCm39)	Y110*	probably null	Het
D630023F18Rik	T	A	1: 65,147,899 (GRCm39)	N218I	probably damaging	Het
Dchs1	A	T	7: 105,408,212 (GRCm39)	H1873Q	probably benign	Het
Dnah1	T	C	14: 31,012,396 (GRCm39)	D1901G	probably damaging	Het
Eddm3b	T	A	14: 51,354,110 (GRCm39)	S33T	probably damaging	Het
Efcc1	A	G	6: 87,728,768 (GRCm39)	T408A	probably benign	Het
Epas1	A	G	17: 87,134,920 (GRCm39)	T518A	probably benign	Het
Ergic2	A	T	6: 148,104,005 (GRCm39)	M84K	probably damaging	Het
Ergic3	T	C	2: 155,853,160 (GRCm39)	V201A	probably benign	Het
Espn	C	A	4: 152,223,278 (GRCm39)	W258L	probably damaging	Het
Gm45337	A	G	7: 141,697,817 (GRCm39)	S156P	unknown	Het
Hmcn2	T	C	2: 31,244,741 (GRCm39)	Y733H	probably damaging	Het
Itga2	C	T	13: 115,009,866 (GRCm39)	G363D	probably damaging	Het
Kcnk5	T	C	14: 20,192,046 (GRCm39)	R372G	probably benign	Het
Khdc4	G	T	3: 88,617,219 (GRCm39)	S506I	probably damaging	Het
Kif11	T	A	19: 37,386,602 (GRCm39)	D387E	probably damaging	Het
Lrrn2	A	C	1: 132,866,104 (GRCm39)	I390L	probably benign	Het
Muc16	C	T	9: 18,558,529 (GRCm39)	R2588K	unknown	Het
Or3a10	A	G	11: 73,935,782 (GRCm39)	F106S	possibly damaging	Het
Or5b99	A	T	19: 12,976,445 (GRCm39)	I32F	probably damaging	Het
Or7g29	A	G	9: 19,286,262 (GRCm39)	I305T	probably benign	Het
Osbpl8	T	C	10: 111,103,530 (GRCm39)	I178T	probably damaging	Het
Pak2	A	T	16: 31,852,729 (GRCm39)		probably benign	Het
Perp	A	T	10: 18,729,326 (GRCm39)	I86F	possibly damaging	Het
Plpp1	A	T	13: 112,993,511 (GRCm39)	I149F	probably damaging	Het
Polr1f	T	A	12: 33,483,526 (GRCm39)	Y93N	probably damaging	Het
Preb	A	G	5: 31,115,671 (GRCm39)	S220P	probably damaging	Het
Prrt4	T	C	6: 29,177,665 (GRCm39)	T35A	probably damaging	Het
Ror2	T	A	13: 53,286,032 (GRCm39)	I73F	possibly damaging	Het
Rpl36-ps4	A	G	17: 88,228,574 (GRCm39)	T40A	probably damaging	Het
Rsbn1	A	T	3: 103,868,830 (GRCm39)	H623L	possibly damaging	Het
Sap30	C	A	8: 57,940,456 (GRCm39)	A49S	possibly damaging	Het
Sf3b3	T	C	8: 111,550,075 (GRCm39)	T648A	probably benign	Het
Sh3pxd2a	T	C	19: 47,361,882 (GRCm39)	R83G	probably damaging	Het
Slc35e1	A	T	8: 73,246,042 (GRCm39)	I130N	probably damaging	Het
Smchd1	C	T	17: 71,743,767 (GRCm39)	R466Q	probably damaging	Het
Smgc	C	T	15: 91,722,565 (GRCm39)		probably benign	Het
Stk32b	A	T	5: 37,612,341 (GRCm39)	N348K	possibly damaging	Het
Sult2a1	A	G	7: 13,530,342 (GRCm39)	F266L	probably damaging	Het
Sult2a2	A	T	7: 13,467,484 (GRCm39)	M1L	probably damaging	Het
Telo2	A	G	17: 25,332,085 (GRCm39)	V161A	probably benign	Het
Tmed11	C	T	5: 108,925,293 (GRCm39)	R173H	probably damaging	Het
Trappc6a	G	A	7: 19,249,923 (GRCm39)		probably benign	Het
Vmn2r5	A	T	3: 64,398,522 (GRCm39)	V819D	probably damaging	Het
Vmn2r74	A	G	7: 85,606,569 (GRCm39)	I259T	probably damaging	Het
Vps13a	A	G	19: 16,723,340 (GRCm39)	I286T	probably damaging	Het
Vsig10l	A	T	7: 43,117,623 (GRCm39)	I739F	possibly damaging	Het
Zfyve1	A	C	12: 83,594,802 (GRCm39)	V730G	probably benign	Het
Zfyve9	A	T	4: 108,499,288 (GRCm39)	D1284E	possibly damaging	Het
Zmym4	C	T	4: 126,758,060 (GRCm39)	R1498H	probably damaging	Het
Znhit2	A	T	19: 6,111,803 (GRCm39)	R183*	probably null	Het

Other mutations in Map2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Map2	APN	1	66,464,490 (GRCm39)	missense	probably damaging	1.00
IGL02135:Map2	APN	1	66,419,920 (GRCm39)	nonsense	probably null
IGL02526:Map2	APN	1	66,419,876 (GRCm39)	missense	possibly damaging	0.94
Annas	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
calliope	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
carthage	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
costas	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
Jacobin	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
Nectar	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
ruby_throat	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
Rufous	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
Speckled	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
Sunbird	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
swift	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
E0370:Map2	UTSW	1	66,455,883 (GRCm39)	unclassified	probably benign
PIT4362001:Map2	UTSW	1	66,451,677 (GRCm39)	missense	probably benign	0.05
R0067:Map2	UTSW	1	66,452,322 (GRCm39)	missense	probably benign	0.04
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0268:Map2	UTSW	1	66,419,881 (GRCm39)	nonsense	probably null
R0302:Map2	UTSW	1	66,453,987 (GRCm39)	missense	probably benign	0.15
R0305:Map2	UTSW	1	66,452,253 (GRCm39)	missense	probably benign	0.00
R0409:Map2	UTSW	1	66,472,739 (GRCm39)	missense	probably damaging	1.00
R0561:Map2	UTSW	1	66,464,656 (GRCm39)	missense	probably damaging	1.00
R0674:Map2	UTSW	1	66,452,361 (GRCm39)	missense	probably damaging	1.00
R0738:Map2	UTSW	1	66,464,348 (GRCm39)	splice site	probably benign
R0893:Map2	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
R1305:Map2	UTSW	1	66,464,554 (GRCm39)	missense	probably damaging	1.00
R1534:Map2	UTSW	1	66,452,339 (GRCm39)	missense	probably benign	0.33
R1632:Map2	UTSW	1	66,454,245 (GRCm39)	missense	possibly damaging	0.60
R1682:Map2	UTSW	1	66,454,781 (GRCm39)	splice site	probably null
R1774:Map2	UTSW	1	66,453,233 (GRCm39)	missense	probably damaging	1.00
R2014:Map2	UTSW	1	66,455,295 (GRCm39)	missense	possibly damaging	0.55
R2017:Map2	UTSW	1	66,451,958 (GRCm39)	missense	probably damaging	1.00
R2050:Map2	UTSW	1	66,453,473 (GRCm39)	missense	probably damaging	0.98
R2093:Map2	UTSW	1	66,438,599 (GRCm39)	missense	probably damaging	1.00
R2214:Map2	UTSW	1	66,459,345 (GRCm39)	missense	probably damaging	0.99
R2284:Map2	UTSW	1	66,453,227 (GRCm39)	missense	probably damaging	1.00
R3011:Map2	UTSW	1	66,453,771 (GRCm39)	missense	probably damaging	1.00
R3105:Map2	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
R3708:Map2	UTSW	1	66,455,714 (GRCm39)	unclassified	probably benign
R3709:Map2	UTSW	1	66,455,015 (GRCm39)	nonsense	probably null
R3729:Map2	UTSW	1	66,451,605 (GRCm39)	missense	possibly damaging	0.80
R3760:Map2	UTSW	1	66,478,077 (GRCm39)	missense	probably damaging	1.00
R3788:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R3789:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R4003:Map2	UTSW	1	66,454,899 (GRCm39)	missense	probably damaging	1.00
R4120:Map2	UTSW	1	66,455,063 (GRCm39)	missense	probably damaging	1.00
R4172:Map2	UTSW	1	66,452,759 (GRCm39)	missense	possibly damaging	0.89
R4198:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4200:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4205:Map2	UTSW	1	66,464,449 (GRCm39)	missense	probably damaging	1.00
R4613:Map2	UTSW	1	66,464,628 (GRCm39)	missense	probably damaging	1.00
R4700:Map2	UTSW	1	66,449,796 (GRCm39)	missense	probably damaging	0.96
R4974:Map2	UTSW	1	66,452,664 (GRCm39)	missense	probably benign	0.15
R5007:Map2	UTSW	1	66,452,448 (GRCm39)	missense	possibly damaging	0.86
R5039:Map2	UTSW	1	66,477,955 (GRCm39)	missense	probably damaging	1.00
R5237:Map2	UTSW	1	66,478,169 (GRCm39)	unclassified	probably benign
R5313:Map2	UTSW	1	66,464,538 (GRCm39)	missense	probably damaging	1.00
R5455:Map2	UTSW	1	66,438,550 (GRCm39)	missense	probably damaging	1.00
R5490:Map2	UTSW	1	66,452,292 (GRCm39)	missense	probably damaging	1.00
R5517:Map2	UTSW	1	66,454,415 (GRCm39)	missense	probably benign	0.00
R5532:Map2	UTSW	1	66,453,779 (GRCm39)	missense	probably damaging	1.00
R5583:Map2	UTSW	1	66,455,196 (GRCm39)	missense	probably damaging	1.00
R5764:Map2	UTSW	1	66,454,034 (GRCm39)	missense	probably damaging	0.99
R5996:Map2	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
R6058:Map2	UTSW	1	66,454,573 (GRCm39)	missense	probably benign	0.05
R6199:Map2	UTSW	1	66,464,637 (GRCm39)	missense	probably damaging	1.00
R6208:Map2	UTSW	1	66,470,749 (GRCm39)	missense	probably damaging	1.00
R6276:Map2	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
R6378:Map2	UTSW	1	66,454,488 (GRCm39)	missense	probably damaging	1.00
R6424:Map2	UTSW	1	66,453,946 (GRCm39)	missense	possibly damaging	0.67
R6743:Map2	UTSW	1	66,454,766 (GRCm39)	missense	probably benign	0.04
R6837:Map2	UTSW	1	66,453,731 (GRCm39)	missense	probably damaging	1.00
R6901:Map2	UTSW	1	66,460,932 (GRCm39)	missense	possibly damaging	0.94
R6984:Map2	UTSW	1	66,454,395 (GRCm39)	missense	possibly damaging	0.90
R6989:Map2	UTSW	1	66,454,065 (GRCm39)	missense	probably benign	0.00
R7001:Map2	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
R7055:Map2	UTSW	1	66,455,983 (GRCm39)	missense	probably damaging	1.00
R7094:Map2	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
R7106:Map2	UTSW	1	66,449,903 (GRCm39)	missense	possibly damaging	0.92
R7182:Map2	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
R7235:Map2	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
R7424:Map2	UTSW	1	66,453,983 (GRCm39)	missense	possibly damaging	0.69
R7473:Map2	UTSW	1	66,454,617 (GRCm39)	missense	probably damaging	1.00
R7642:Map2	UTSW	1	66,452,466 (GRCm39)	missense	probably benign	0.01
R7660:Map2	UTSW	1	66,453,536 (GRCm39)	missense	probably damaging	1.00
R7673:Map2	UTSW	1	66,452,933 (GRCm39)	missense	probably benign	0.03
R7768:Map2	UTSW	1	66,453,642 (GRCm39)	missense	possibly damaging	0.94
R7796:Map2	UTSW	1	66,455,654 (GRCm39)	splice site	probably null
R7834:Map2	UTSW	1	66,455,647 (GRCm39)	missense	probably damaging	1.00
R7842:Map2	UTSW	1	66,455,570 (GRCm39)	missense	probably benign
R7955:Map2	UTSW	1	66,452,875 (GRCm39)	missense	probably damaging	1.00
R8056:Map2	UTSW	1	66,454,779 (GRCm39)	missense	probably damaging	0.99
R8118:Map2	UTSW	1	66,464,550 (GRCm39)	missense	probably damaging	1.00
R8135:Map2	UTSW	1	66,452,828 (GRCm39)	missense	probably damaging	1.00
R8152:Map2	UTSW	1	66,453,902 (GRCm39)	missense	probably benign	0.00
R8223:Map2	UTSW	1	66,464,649 (GRCm39)	missense	probably damaging	1.00
R8329:Map2	UTSW	1	66,454,272 (GRCm39)	missense	probably benign	0.01
R8344:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8475:Map2	UTSW	1	66,453,164 (GRCm39)	missense	probably damaging	1.00
R8548:Map2	UTSW	1	66,452,499 (GRCm39)	missense	probably damaging	1.00
R8680:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8713:Map2	UTSW	1	66,453,781 (GRCm39)	missense	probably damaging	1.00
R8745:Map2	UTSW	1	66,452,556 (GRCm39)	missense	probably benign	0.00
R8786:Map2	UTSW	1	66,472,755 (GRCm39)	critical splice donor site	probably benign
R8790:Map2	UTSW	1	66,477,997 (GRCm39)	missense	probably damaging	1.00
R8874:Map2	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
R8887:Map2	UTSW	1	66,454,758 (GRCm39)	missense	possibly damaging	0.48
R8950:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8998:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R8999:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R9072:Map2	UTSW	1	66,453,312 (GRCm39)	missense	probably damaging	1.00
R9088:Map2	UTSW	1	66,453,773 (GRCm39)	missense	probably damaging	1.00
R9089:Map2	UTSW	1	66,452,098 (GRCm39)	missense	probably benign	0.09
R9106:Map2	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
R9112:Map2	UTSW	1	66,472,723 (GRCm39)	nonsense	probably null
R9120:Map2	UTSW	1	66,453,218 (GRCm39)	missense	probably damaging	1.00
R9161:Map2	UTSW	1	66,477,503 (GRCm39)	missense	possibly damaging	0.65
R9464:Map2	UTSW	1	66,454,497 (GRCm39)	missense	probably damaging	1.00
R9589:Map2	UTSW	1	66,449,753 (GRCm39)	missense	probably benign
V8831:Map2	UTSW	1	66,455,004 (GRCm39)	missense	probably damaging	1.00
Z1177:Map2	UTSW	1	66,477,520 (GRCm39)	missense	probably damaging	0.96
Z1177:Map2	UTSW	1	66,419,839 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CACTTGTCCTGAAATACGATTGC -3'
(R):5'- AATAGGTGCCCTGTGACCTG -3'

Sequencing Primer
(F):5'- CTTTCCATAAATGCTTTCCAAAAACC -3'
(R):5'- TGTGACCTGTGCTCCCCAAAG -3'

Posted On

2021-08-31