Mutagenetix > Incidental Mutations

Incidental Mutation 'R8948:Olfr847'

681459

Institutional Source

Beutler Lab

Gene Symbol

Olfr847

Ensembl Gene

ENSMUSG00000059821

Gene Name

olfactory receptor 847

Synonyms

MOR149-2, GA_x6K02T2PVTD-13113073-13112135

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.132) question?

Stock #

R8948 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19373668-19378669 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19374966 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 305 (I305T)

Ref Sequence

ENSEMBL: ENSMUSP00000151112 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079620] [ENSMUST00000216839]

AlphaFold

Q8VFF4

Predicted Effect

probably benign
Transcript: ENSMUST00000079620
AA Change: I305T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000078568
Gene: ENSMUSG00000059821
AA Change: I305T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.9e-56	PFAM
Pfam:7tm_1	41	290	2.1e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216839
AA Change: I305T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810403A07Rik	G	T	3: 88,709,912	S506I	probably damaging	Het
Abat	T	C	16: 8,600,941	I102T	possibly damaging	Het
Abca9	T	C	11: 110,163,380		probably null	Het
Ago3	T	C	4: 126,350,322		probably null	Het
Akap1	G	T	11: 88,844,273	A75E	probably damaging	Het
Atad2b	C	A	12: 4,991,012	A227E	possibly damaging	Het
Bmpr1a	G	T	14: 34,441,191	H81N	possibly damaging	Het
Camkv	C	T	9: 107,947,097	T236I	probably damaging	Het
Ccdc30	T	C	4: 119,324,161	E601G	probably benign	Het
Ccin	G	A	4: 43,984,064	R157H	probably damaging	Het
Cdkn3	C	T	14: 46,767,323	P114L	probably damaging	Het
Col6a2	C	T	10: 76,610,693	G352D	probably damaging	Het
Csf2rb	A	T	15: 78,348,320	D609V	probably benign	Het
Ctc1	T	G	11: 69,026,349	Y110*	probably null	Het
D630023F18Rik	T	A	1: 65,108,740	N218I	probably damaging	Het
Dchs1	A	T	7: 105,759,005	H1873Q	probably benign	Het
Dnah1	T	C	14: 31,290,439	D1901G	probably damaging	Het
Eddm3b	T	A	14: 51,116,653	S33T	probably damaging	Het
Efcc1	A	G	6: 87,751,786	T408A	probably benign	Het
Epas1	A	G	17: 86,827,492	T518A	probably benign	Het
Ergic2	A	T	6: 148,202,507	M84K	probably damaging	Het
Ergic3	T	C	2: 156,011,240	V201A	probably benign	Het
Espn	C	A	4: 152,138,821	W258L	probably damaging	Het
Gm45337	A	G	7: 142,144,080	S156P	unknown	Het
Hmcn2	T	C	2: 31,354,729	Y733H	probably damaging	Het
Itga2	C	T	13: 114,873,330	G363D	probably damaging	Het
Kcnk5	T	C	14: 20,141,978	R372G	probably benign	Het
Kif11	T	A	19: 37,398,154	D387E	probably damaging	Het
Lrrn2	A	C	1: 132,938,366	I390L	probably benign	Het
Map2	G	T	1: 66,380,525	R5L	probably damaging	Het
Muc16	C	T	9: 18,647,233	R2588K	unknown	Het
Olfr139	A	G	11: 74,044,956	F106S	possibly damaging	Het
Olfr1451	A	T	19: 12,999,081	I32F	probably damaging	Het
Osbpl8	T	C	10: 111,267,669	I178T	probably damaging	Het
Perp	A	T	10: 18,853,578	I86F	possibly damaging	Het
Plpp1	A	T	13: 112,856,977	I149F	probably damaging	Het
Preb	A	G	5: 30,958,327	S220P	probably damaging	Het
Prrt4	T	C	6: 29,177,666	T35A	probably damaging	Het
Ror2	T	A	13: 53,131,996	I73F	possibly damaging	Het
Rpl36-ps4	A	G	17: 87,921,146	T40A	probably damaging	Het
Rsbn1	A	T	3: 103,961,514	H623L	possibly damaging	Het
Sap30	C	A	8: 57,487,422	A49S	possibly damaging	Het
Sf3b3	T	C	8: 110,823,443	T648A	probably benign	Het
Sh3pxd2a	T	C	19: 47,373,443	R83G	probably damaging	Het
Slc35e1	A	T	8: 72,492,198	I130N	probably damaging	Het
Smchd1	C	T	17: 71,436,772	R466Q	probably damaging	Het
Smgc	C	T	15: 91,838,362		probably benign	Het
Stk32b	A	T	5: 37,454,997	N348K	possibly damaging	Het
Sult2a1	A	G	7: 13,796,417	F266L	probably damaging	Het
Sult2a2	A	T	7: 13,733,559	M1L	probably damaging	Het
Telo2	A	G	17: 25,113,111	V161A	probably benign	Het
Tmed11	C	T	5: 108,777,427	R173H	probably damaging	Het
Trappc6a	G	A	7: 19,515,998		probably benign	Het
Twistnb	T	A	12: 33,433,527	Y93N	probably damaging	Het
Vmn2r5	A	T	3: 64,491,101	V819D	probably damaging	Het
Vmn2r74	A	G	7: 85,957,361	I259T	probably damaging	Het
Vps13a	A	G	19: 16,745,976	I286T	probably damaging	Het
Vsig10l	A	T	7: 43,468,199	I739F	possibly damaging	Het
Zfyve1	A	C	12: 83,548,028	V730G	probably benign	Het
Zfyve9	A	T	4: 108,642,091	D1284E	possibly damaging	Het
Zmym4	C	T	4: 126,864,267	R1498H	probably damaging	Het
Znhit2	A	T	19: 6,061,773	R183*	probably null	Het

Other mutations in Olfr847

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01150:Olfr847	APN	9	19375239	missense	probably damaging	1.00
IGL01293:Olfr847	APN	9	19375336	missense	probably benign	0.23
IGL01879:Olfr847	APN	9	19375407	nonsense	probably null
IGL03298:Olfr847	APN	9	19375062	missense	probably damaging	1.00
R1350:Olfr847	UTSW	9	19375414	missense	possibly damaging	0.94
R1400:Olfr847	UTSW	9	19375062	missense	probably damaging	0.98
R2894:Olfr847	UTSW	9	19375292	nonsense	probably null
R4468:Olfr847	UTSW	9	19375648	missense	probably benign	0.00
R4694:Olfr847	UTSW	9	19375398	missense	probably damaging	1.00
R4791:Olfr847	UTSW	9	19375809	missense	probably benign	0.28
R4794:Olfr847	UTSW	9	19375545	missense	probably benign	0.00
R5517:Olfr847	UTSW	9	19375767	missense	probably damaging	1.00
R5599:Olfr847	UTSW	9	19375629	missense	possibly damaging	0.77
R5777:Olfr847	UTSW	9	19375718	missense	probably benign	0.29
R6505:Olfr847	UTSW	9	19374941	makesense	probably null
R6509:Olfr847	UTSW	9	19375143	missense	probably benign
R7246:Olfr847	UTSW	9	19375465	nonsense	probably null
R7659:Olfr847	UTSW	9	19375558	missense	probably benign	0.03
R7789:Olfr847	UTSW	9	19375065	missense	probably benign	0.33
R7886:Olfr847	UTSW	9	19375906	splice site	probably null
R9326:Olfr847	UTSW	9	19375050	missense	probably damaging	1.00
Z1088:Olfr847	UTSW	9	19375684	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCATCTGGTCTCATGAGGAAATTAC -3'
(R):5'- ATCCTTATTCTATGGTGCAGCAG -3'

Sequencing Primer
(F):5'- CATGAGGAAATTACATTTGTGTGCTC -3'
(R):5'- CAGCAGTGGGAGTATACATCTGTTC -3'

Posted On

2021-08-31