Mutagenetix > Incidental Mutation

Incidental Mutation 'R8950:Vmn2r5'

681552

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r5

Ensembl Gene

ENSMUSG00000068999

Gene Name

vomeronasal 2, receptor 5

Synonyms

EG667060

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R8950 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64398242-64417156 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64398522 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 819 (V819D)

Ref Sequence

ENSEMBL: ENSMUSP00000135592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170270] [ENSMUST00000177184]

AlphaFold

K7N788

Predicted Effect

probably damaging
Transcript: ENSMUST00000170270
AA Change: V732D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000131925
Gene: ENSMUSG00000068999
AA Change: V732D

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	9.9e-78	PFAM
Pfam:NCD3G	458	511	8.5e-18	PFAM
Pfam:7tm_3	542	779	2.3e-74	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000177184
AA Change: V819D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000135592
Gene: ENSMUSG00000068999
AA Change: V819D

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	86	503	1.8e-80	PFAM
Pfam:NCD3G	545	598	1e-14	PFAM
Pfam:7tm_3	631	865	1.4e-45	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	T	C	7: 119,823,595 (GRCm39)	F374S	possibly damaging	Het
Abca14	T	A	7: 119,823,644 (GRCm39)	Y390*	probably null	Het
Abca9	T	C	11: 110,054,206 (GRCm39)		probably null	Het
Anxa1	G	T	19: 20,352,662 (GRCm39)	S304*	probably null	Het
Aspm	G	A	1: 139,406,690 (GRCm39)	R1859Q	probably damaging	Het
Atp13a5	A	T	16: 29,197,314 (GRCm39)	N14K	probably damaging	Het
Bmpr1a	G	T	14: 34,163,148 (GRCm39)	H81N	possibly damaging	Het
Camkv	C	T	9: 107,824,296 (GRCm39)	T236I	probably damaging	Het
Carm1	T	A	9: 21,490,789 (GRCm39)	M219K	probably damaging	Het
Ccdc162	T	C	10: 41,474,507 (GRCm39)	R1352G	probably benign	Het
Cd200l1	A	T	16: 45,263,007 (GRCm39)	V44D	possibly damaging	Het
Cib1	A	G	7: 79,878,211 (GRCm39)	L89P	probably damaging	Het
Col6a5	C	T	9: 105,822,833 (GRCm39)	E175K	unknown	Het
Crebbp	A	G	16: 4,031,023 (GRCm39)	F22S	probably damaging	Het
Csf2rb	A	T	15: 78,232,520 (GRCm39)	D609V	probably benign	Het
Csn1s1	T	C	5: 87,824,482 (GRCm39)		probably null	Het
Dchs1	A	T	7: 105,408,212 (GRCm39)	H1873Q	probably benign	Het
Dnah1	T	C	14: 31,012,396 (GRCm39)	D1901G	probably damaging	Het
Dock10	G	T	1: 80,519,016 (GRCm39)	T1235N	probably benign	Het
Eddm3b	T	A	14: 51,354,110 (GRCm39)	S33T	probably damaging	Het
Fam151b	A	T	13: 92,610,567 (GRCm39)	M74K	probably damaging	Het
Fat1	T	A	8: 45,476,158 (GRCm39)	F1735I	probably damaging	Het
Fcgbpl1	A	G	7: 27,863,751 (GRCm39)	R2508G	probably benign	Het
Fip1l1	T	G	5: 74,756,524 (GRCm39)	S505R	probably damaging	Het
Gm21886	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	18: 80,133,040 (GRCm39)		probably benign	Het
Gpr180	A	G	14: 118,395,452 (GRCm39)	I295V	probably benign	Het
Gtf3c2	G	A	5: 31,331,151 (GRCm39)	T175I	probably damaging	Het
Hmcn2	T	C	2: 31,244,741 (GRCm39)	Y733H	probably damaging	Het
Irs1	T	A	1: 82,264,652 (GRCm39)	Q1188L	probably benign	Het
Kcnk5	T	C	14: 20,192,046 (GRCm39)	R372G	probably benign	Het
Kdm5b	T	A	1: 134,541,664 (GRCm39)	N746K	possibly damaging	Het
Kdm7a	T	C	6: 39,123,903 (GRCm39)	I754V	probably benign	Het
Mad2l1bp	G	A	17: 46,458,891 (GRCm39)	T213I	possibly damaging	Het
Map2	G	T	1: 66,419,684 (GRCm39)	R5L	probably damaging	Het
Map4	G	A	9: 109,901,702 (GRCm39)	V781M	possibly damaging	Het
Mbd2	A	G	18: 70,713,864 (GRCm39)	D231G	probably damaging	Het
Negr1	C	A	3: 156,721,906 (GRCm39)	P160Q	probably damaging	Het
Nfya	A	T	17: 48,700,489 (GRCm39)		probably benign	Het
Nlk	T	C	11: 78,586,758 (GRCm39)	Q112R	probably benign	Het
Obscn	C	A	11: 58,947,009 (GRCm39)	A4339S	probably damaging	Het
Or3a10	A	G	11: 73,935,782 (GRCm39)	F106S	possibly damaging	Het
Or5b99	A	T	19: 12,976,445 (GRCm39)	I32F	probably damaging	Het
Pcdhb12	A	G	18: 37,570,590 (GRCm39)	T579A	probably benign	Het
Pcdhb2	G	A	18: 37,429,715 (GRCm39)	V563M	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Piezo1	T	C	8: 123,208,729 (GRCm39)	R2545G	probably benign	Het
Plcb1	A	G	2: 135,179,439 (GRCm39)	N616D	probably damaging	Het
Pogk	A	T	1: 166,226,394 (GRCm39)	F586I	probably damaging	Het
Polr1f	T	A	12: 33,483,526 (GRCm39)	Y93N	probably damaging	Het
Prkg2	T	A	5: 99,119,815 (GRCm39)	I435F	possibly damaging	Het
Prrt4	T	C	6: 29,177,665 (GRCm39)	T35A	probably damaging	Het
Rad17	A	G	13: 100,767,576 (GRCm39)	S342P	probably damaging	Het
Rpl36-ps4	A	G	17: 88,228,574 (GRCm39)	T40A	probably damaging	Het
Skint5	T	C	4: 113,374,349 (GRCm39)	T1247A	unknown	Het
Slc22a14	A	G	9: 118,998,778 (GRCm39)	L182P	possibly damaging	Het
Slc35b3	T	C	13: 39,138,097 (GRCm39)	D104G	probably damaging	Het
Sult2a1	A	G	7: 13,530,342 (GRCm39)	F266L	probably damaging	Het
Sult2a2	A	T	7: 13,467,484 (GRCm39)	M1L	probably damaging	Het
Tat	T	A	8: 110,718,337 (GRCm39)	I74N	probably damaging	Het
Trappc6a	G	T	7: 19,249,923 (GRCm39)		probably benign	Het
Usp44	A	G	10: 93,682,129 (GRCm39)	E193G	possibly damaging	Het
Virma	C	T	4: 11,519,047 (GRCm39)	Q765*	probably null	Het
Vmn1r46	T	C	6: 89,954,053 (GRCm39)	S301P	probably damaging	Het
Vmn2r116	A	T	17: 23,620,467 (GRCm39)	I734F	probably damaging	Het
Vmn2r74	A	G	7: 85,606,569 (GRCm39)	I259T	probably damaging	Het
Vps13a	A	G	19: 16,723,340 (GRCm39)	I286T	probably damaging	Het
Wdr73	T	C	7: 80,550,131 (GRCm39)	I55V	probably benign	Het
Znhit2	A	T	19: 6,111,803 (GRCm39)	R183*	probably null	Het

Other mutations in Vmn2r5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00742:Vmn2r5	APN	3	64,398,834 (GRCm39)	missense	possibly damaging	0.67
IGL01139:Vmn2r5	APN	3	64,398,826 (GRCm39)	missense	probably benign	0.28
IGL03293:Vmn2r5	APN	3	64,398,747 (GRCm39)	missense	probably benign	0.01
PIT4581001:Vmn2r5	UTSW	3	64,411,348 (GRCm39)	nonsense	probably null
R0063:Vmn2r5	UTSW	3	64,411,221 (GRCm39)	missense	probably benign	0.01
R0142:Vmn2r5	UTSW	3	64,400,009 (GRCm39)	missense	probably damaging	1.00
R0193:Vmn2r5	UTSW	3	64,398,951 (GRCm39)	missense	possibly damaging	0.91
R0219:Vmn2r5	UTSW	3	64,411,734 (GRCm39)	missense	probably damaging	0.99
R0313:Vmn2r5	UTSW	3	64,411,248 (GRCm39)	missense	probably benign
R0620:Vmn2r5	UTSW	3	64,411,235 (GRCm39)	nonsense	probably null
R0726:Vmn2r5	UTSW	3	64,411,186 (GRCm39)	missense	probably benign	0.01
R1073:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R1549:Vmn2r5	UTSW	3	64,411,421 (GRCm39)	missense	probably damaging	1.00
R1581:Vmn2r5	UTSW	3	64,398,640 (GRCm39)	missense	probably damaging	1.00
R1624:Vmn2r5	UTSW	3	64,417,116 (GRCm39)	missense	probably benign	0.19
R1973:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1975:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1977:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R2189:Vmn2r5	UTSW	3	64,417,014 (GRCm39)	missense	probably benign	0.27
R2484:Vmn2r5	UTSW	3	64,411,392 (GRCm39)	missense	possibly damaging	0.94
R4573:Vmn2r5	UTSW	3	64,411,339 (GRCm39)	missense	probably damaging	0.98
R5243:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5384:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5385:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5587:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5588:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5589:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5590:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5739:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R6113:Vmn2r5	UTSW	3	64,398,820 (GRCm39)	missense	probably benign	0.03
R6739:Vmn2r5	UTSW	3	64,398,637 (GRCm39)	missense	probably damaging	0.97
R7106:Vmn2r5	UTSW	3	64,399,104 (GRCm39)	missense	probably benign	0.05
R7304:Vmn2r5	UTSW	3	64,411,671 (GRCm39)	missense	probably damaging	0.97
R7402:Vmn2r5	UTSW	3	64,403,176 (GRCm39)	missense	probably benign	0.05
R7477:Vmn2r5	UTSW	3	64,399,060 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r5	UTSW	3	64,411,825 (GRCm39)	missense	probably damaging	1.00
R7678:Vmn2r5	UTSW	3	64,416,943 (GRCm39)	missense	probably benign
R7874:Vmn2r5	UTSW	3	64,398,453 (GRCm39)	missense	probably damaging	1.00
R8110:Vmn2r5	UTSW	3	64,398,709 (GRCm39)	missense	probably benign	0.00
R8223:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R8767:Vmn2r5	UTSW	3	64,415,103 (GRCm39)	missense	possibly damaging	0.79
R8896:Vmn2r5	UTSW	3	64,411,203 (GRCm39)	missense	probably benign	0.00
R8948:Vmn2r5	UTSW	3	64,398,522 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r5	UTSW	3	64,398,564 (GRCm39)	missense	probably damaging	1.00
R9012:Vmn2r5	UTSW	3	64,411,915 (GRCm39)	missense	probably damaging	1.00
R9109:Vmn2r5	UTSW	3	64,411,411 (GRCm39)	missense	possibly damaging	0.58
R9126:Vmn2r5	UTSW	3	64,399,159 (GRCm39)	missense	probably benign	0.13
R9192:Vmn2r5	UTSW	3	64,398,938 (GRCm39)	nonsense	probably null
R9221:Vmn2r5	UTSW	3	64,411,721 (GRCm39)	nonsense	probably null
R9375:Vmn2r5	UTSW	3	64,411,316 (GRCm39)	missense	probably damaging	1.00
R9529:Vmn2r5	UTSW	3	64,400,018 (GRCm39)	missense	probably benign	0.06
Z1088:Vmn2r5	UTSW	3	64,416,963 (GRCm39)	missense	probably benign	0.02
Z1177:Vmn2r5	UTSW	3	64,398,472 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTTCCACCAACAGTTTCATCAG -3'
(R):5'- TACTTGGTGTTGGAGCCTCC -3'

Sequencing Primer
(F):5'- ACCAACAGTTTCATCAGTGTTC -3'
(R):5'- GGAGCCTCCAAAGATGTTCAAG -3'

Posted On

2021-08-31