Incidental Mutation 'R8950:Anxa1'
ID 681612
Institutional Source Beutler Lab
Gene Symbol Anxa1
Ensembl Gene ENSMUSG00000024659
Gene Name annexin A1
Synonyms Anx-1, Anx-A1, Lpc-1, Lpc1, C430014K04Rik
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.300) question?
Stock # R8950 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 20350798-20368035 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) G to T at 20352662 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Stop codon at position 304 (S304*)
Ref Sequence ENSEMBL: ENSMUSP00000025561 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025561]
AlphaFold P10107
Predicted Effect probably null
Transcript: ENSMUST00000025561
AA Change: S304*
SMART Domains Protein: ENSMUSP00000025561
Gene: ENSMUSG00000024659
AA Change: S304*

DomainStartEndE-ValueType
ANX 59 111 4.45e-21 SMART
ANX 131 183 3.9e-26 SMART
ANX 215 267 4.86e-13 SMART
ANX 290 342 2.26e-22 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.4%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mutations in this gene result in increased inflammatory response and decreased macrophage activity. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca14 T C 7: 119,823,595 (GRCm39) F374S possibly damaging Het
Abca14 T A 7: 119,823,644 (GRCm39) Y390* probably null Het
Abca9 T C 11: 110,054,206 (GRCm39) probably null Het
Aspm G A 1: 139,406,690 (GRCm39) R1859Q probably damaging Het
Atp13a5 A T 16: 29,197,314 (GRCm39) N14K probably damaging Het
Bmpr1a G T 14: 34,163,148 (GRCm39) H81N possibly damaging Het
Camkv C T 9: 107,824,296 (GRCm39) T236I probably damaging Het
Carm1 T A 9: 21,490,789 (GRCm39) M219K probably damaging Het
Ccdc162 T C 10: 41,474,507 (GRCm39) R1352G probably benign Het
Cd200l1 A T 16: 45,263,007 (GRCm39) V44D possibly damaging Het
Cib1 A G 7: 79,878,211 (GRCm39) L89P probably damaging Het
Col6a5 C T 9: 105,822,833 (GRCm39) E175K unknown Het
Crebbp A G 16: 4,031,023 (GRCm39) F22S probably damaging Het
Csf2rb A T 15: 78,232,520 (GRCm39) D609V probably benign Het
Csn1s1 T C 5: 87,824,482 (GRCm39) probably null Het
Dchs1 A T 7: 105,408,212 (GRCm39) H1873Q probably benign Het
Dnah1 T C 14: 31,012,396 (GRCm39) D1901G probably damaging Het
Dock10 G T 1: 80,519,016 (GRCm39) T1235N probably benign Het
Eddm3b T A 14: 51,354,110 (GRCm39) S33T probably damaging Het
Fam151b A T 13: 92,610,567 (GRCm39) M74K probably damaging Het
Fat1 T A 8: 45,476,158 (GRCm39) F1735I probably damaging Het
Fcgbpl1 A G 7: 27,863,751 (GRCm39) R2508G probably benign Het
Fip1l1 T G 5: 74,756,524 (GRCm39) S505R probably damaging Het
Gm21886 ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG 18: 80,133,040 (GRCm39) probably benign Het
Gpr180 A G 14: 118,395,452 (GRCm39) I295V probably benign Het
Gtf3c2 G A 5: 31,331,151 (GRCm39) T175I probably damaging Het
Hmcn2 T C 2: 31,244,741 (GRCm39) Y733H probably damaging Het
Irs1 T A 1: 82,264,652 (GRCm39) Q1188L probably benign Het
Kcnk5 T C 14: 20,192,046 (GRCm39) R372G probably benign Het
Kdm5b T A 1: 134,541,664 (GRCm39) N746K possibly damaging Het
Kdm7a T C 6: 39,123,903 (GRCm39) I754V probably benign Het
Mad2l1bp G A 17: 46,458,891 (GRCm39) T213I possibly damaging Het
Map2 G T 1: 66,419,684 (GRCm39) R5L probably damaging Het
Map4 G A 9: 109,901,702 (GRCm39) V781M possibly damaging Het
Mbd2 A G 18: 70,713,864 (GRCm39) D231G probably damaging Het
Negr1 C A 3: 156,721,906 (GRCm39) P160Q probably damaging Het
Nfya A T 17: 48,700,489 (GRCm39) probably benign Het
Nlk T C 11: 78,586,758 (GRCm39) Q112R probably benign Het
Obscn C A 11: 58,947,009 (GRCm39) A4339S probably damaging Het
Or3a10 A G 11: 73,935,782 (GRCm39) F106S possibly damaging Het
Or5b99 A T 19: 12,976,445 (GRCm39) I32F probably damaging Het
Pcdhb12 A G 18: 37,570,590 (GRCm39) T579A probably benign Het
Pcdhb2 G A 18: 37,429,715 (GRCm39) V563M probably damaging Het
Peg10 GC GCTCC 6: 4,756,452 (GRCm39) probably benign Het
Piezo1 T C 8: 123,208,729 (GRCm39) R2545G probably benign Het
Plcb1 A G 2: 135,179,439 (GRCm39) N616D probably damaging Het
Pogk A T 1: 166,226,394 (GRCm39) F586I probably damaging Het
Polr1f T A 12: 33,483,526 (GRCm39) Y93N probably damaging Het
Prkg2 T A 5: 99,119,815 (GRCm39) I435F possibly damaging Het
Prrt4 T C 6: 29,177,665 (GRCm39) T35A probably damaging Het
Rad17 A G 13: 100,767,576 (GRCm39) S342P probably damaging Het
Rpl36-ps4 A G 17: 88,228,574 (GRCm39) T40A probably damaging Het
Skint5 T C 4: 113,374,349 (GRCm39) T1247A unknown Het
Slc22a14 A G 9: 118,998,778 (GRCm39) L182P possibly damaging Het
Slc35b3 T C 13: 39,138,097 (GRCm39) D104G probably damaging Het
Sult2a1 A G 7: 13,530,342 (GRCm39) F266L probably damaging Het
Sult2a2 A T 7: 13,467,484 (GRCm39) M1L probably damaging Het
Tat T A 8: 110,718,337 (GRCm39) I74N probably damaging Het
Trappc6a G T 7: 19,249,923 (GRCm39) probably benign Het
Usp44 A G 10: 93,682,129 (GRCm39) E193G possibly damaging Het
Virma C T 4: 11,519,047 (GRCm39) Q765* probably null Het
Vmn1r46 T C 6: 89,954,053 (GRCm39) S301P probably damaging Het
Vmn2r116 A T 17: 23,620,467 (GRCm39) I734F probably damaging Het
Vmn2r5 A T 3: 64,398,522 (GRCm39) V819D probably damaging Het
Vmn2r74 A G 7: 85,606,569 (GRCm39) I259T probably damaging Het
Vps13a A G 19: 16,723,340 (GRCm39) I286T probably damaging Het
Wdr73 T C 7: 80,550,131 (GRCm39) I55V probably benign Het
Znhit2 A T 19: 6,111,803 (GRCm39) R183* probably null Het
Other mutations in Anxa1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00592:Anxa1 APN 19 20,355,033 (GRCm39) missense probably benign 0.01
IGL03234:Anxa1 APN 19 20,354,965 (GRCm39) splice site probably benign
R0058:Anxa1 UTSW 19 20,361,141 (GRCm39) missense probably damaging 1.00
R0058:Anxa1 UTSW 19 20,361,141 (GRCm39) missense probably damaging 1.00
R1446:Anxa1 UTSW 19 20,351,103 (GRCm39) missense probably damaging 1.00
R1864:Anxa1 UTSW 19 20,357,053 (GRCm39) missense probably benign 0.00
R2213:Anxa1 UTSW 19 20,360,239 (GRCm39) missense probably damaging 1.00
R4787:Anxa1 UTSW 19 20,351,118 (GRCm39) missense probably damaging 0.98
R5013:Anxa1 UTSW 19 20,360,287 (GRCm39) missense probably benign 0.02
R5453:Anxa1 UTSW 19 20,357,703 (GRCm39) critical splice donor site probably null
R5468:Anxa1 UTSW 19 20,355,847 (GRCm39) missense probably damaging 1.00
R5918:Anxa1 UTSW 19 20,355,857 (GRCm39) splice site probably benign
R6059:Anxa1 UTSW 19 20,355,064 (GRCm39) missense possibly damaging 0.91
R6379:Anxa1 UTSW 19 20,351,079 (GRCm39) makesense probably null
R6394:Anxa1 UTSW 19 20,361,213 (GRCm39) missense probably damaging 1.00
R7049:Anxa1 UTSW 19 20,352,635 (GRCm39) missense probably benign
R8280:Anxa1 UTSW 19 20,352,650 (GRCm39) missense possibly damaging 0.79
Predicted Primers PCR Primer
(F):5'- GCAGTGTATAGCTGTAGCATATCAC -3'
(R):5'- CTAATGCACACCTTCTGCTAATG -3'

Sequencing Primer
(F):5'- AGCTGTAGCATATCACATCTGC -3'
(R):5'- CCCCCGTGGTATGAGTATATG -3'
Posted On 2021-08-31