Mutagenetix > Incidental Mutation

Incidental Mutation 'R8951:Kras'

681637

Institutional Source

Beutler Lab

Gene Symbol

Kras

Ensembl Gene

ENSMUSG00000030265

Gene Name

Kirsten rat sarcoma viral oncogene homolog

Synonyms

Kras2, Kras-2, K-ras, Ki-ras

MMRRC Submission

068788-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8951 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

145162425-145195965 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 145166338 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 188 (M188K)

Ref Sequence

ENSEMBL: ENSMUSP00000032399 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032399] [ENSMUST00000039729] [ENSMUST00000111710] [ENSMUST00000111719] [ENSMUST00000111721] [ENSMUST00000111723] [ENSMUST00000111724] [ENSMUST00000111725] [ENSMUST00000111726] [ENSMUST00000156486] [ENSMUST00000203147]

AlphaFold

P32883

Predicted Effect

probably benign
Transcript: ENSMUST00000032399
AA Change: M188K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000032399
Gene: ENSMUSG00000030265
AA Change: M188K

Domain	Start	End	E-Value	Type
RAS	1	166	1.14e-123	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000039729

SMART Domains

Protein: ENSMUSP00000039433
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111710

SMART Domains

Protein: ENSMUSP00000107339
Gene: ENSMUSG00000030265

Domain	Start	End	E-Value	Type
RAS	1	166	3.7e-123	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000111719

SMART Domains

Protein: ENSMUSP00000107348
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111721

SMART Domains

Protein: ENSMUSP00000107350
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	5.5e-15	PFAM
Pfam:Complex1_LYR_1	7	67	5.5e-15	PFAM
Pfam:Complex1_LYR_2	9	85	9.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111723

SMART Domains

Protein: ENSMUSP00000107352
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111724

SMART Domains

Protein: ENSMUSP00000107353
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111725

SMART Domains

Protein: ENSMUSP00000107354
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000111726

SMART Domains

Protein: ENSMUSP00000107355
Gene: ENSMUSG00000040370

Domain	Start	End	E-Value	Type
Pfam:Complex1_LYR	7	63	2.6e-15	PFAM
Pfam:Complex1_LYR_1	7	74	3.6e-14	PFAM
Pfam:Complex1_LYR_2	9	85	8.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156486

Predicted Effect

SMART Domains

Protein: ENSMUSP00000145294
Gene: ENSMUSG00000030265
AA Change: M75K

Domain	Start	End	E-Value	Type
small_GTPase	1	53	3.1e-8	SMART

Meta Mutation Damage Score

0.2740

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

99% (73/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality, decreased fetal growth, pericardial edema, anemia, and liver hypoplasia. Mice heterozygous for various knock-in alleles exhibit increased tumorigenesis. [provided by MGI curators]

Allele List at MGI

All alleles(26) : Targeted, knock-out(3) Targeted, other(7) Gene trapped(14) Other(2)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam11	T	C	11: 102,665,193 (GRCm39)		probably null	Het
Adcy6	A	T	15: 98,502,140 (GRCm39)	V158E	possibly damaging	Het
Adgrg3	T	C	8: 95,761,362 (GRCm39)		probably benign	Het
Arsb	T	C	13: 93,944,124 (GRCm39)	S272P	probably damaging	Het
Asb10	A	C	5: 24,742,952 (GRCm39)	C260W	probably damaging	Het
Atp13a1	A	G	8: 70,246,484 (GRCm39)	D158G	probably benign	Het
Bcl6	T	C	16: 23,793,704 (GRCm39)	E81G	probably damaging	Het
C7	A	T	15: 5,032,231 (GRCm39)	V660E	probably benign	Het
Cacna1c	T	C	6: 118,590,300 (GRCm39)	D1401G	probably damaging	Het
Ccp110	A	G	7: 118,321,015 (GRCm39)	I223M	possibly damaging	Het
Cilp	A	G	9: 65,180,220 (GRCm39)	E187G	probably benign	Het
Cmc2	T	C	8: 117,637,904 (GRCm39)	N15S	probably damaging	Het
Cnnm3	T	A	1: 36,558,019 (GRCm39)		probably benign	Het
Col27a1	T	G	4: 63,191,311 (GRCm39)	M828R	possibly damaging	Het
Cpm	C	T	10: 117,511,938 (GRCm39)	P294L	probably damaging	Het
Cpt1a	G	C	19: 3,412,211 (GRCm39)	A228P	probably benign	Het
Ctbp1	A	G	5: 33,416,679 (GRCm39)	V43A	probably damaging	Het
Cylc2	A	T	4: 51,229,469 (GRCm39)	E270D	unknown	Het
Cyp46a1	A	C	12: 108,312,348 (GRCm39)	R119S	possibly damaging	Het
Dctn3	A	T	4: 41,719,845 (GRCm39)	I87N	probably damaging	Het
Dhdds	C	T	4: 133,719,857 (GRCm39)	V25I	possibly damaging	Het
Entpd1	A	C	19: 40,727,319 (GRCm39)	N489T	probably damaging	Het
Fam110a	T	C	2: 151,812,461 (GRCm39)	D103G	probably damaging	Het
Foxo6	T	A	4: 120,125,133 (GRCm39)	Q554L	unknown	Het
Gbp5	T	A	3: 142,206,481 (GRCm39)	M55K	probably damaging	Het
Gm20730	T	C	6: 43,058,638 (GRCm39)	Y58C	possibly damaging	Het
Grip1	T	A	10: 119,874,509 (GRCm39)	S679T	possibly damaging	Het
Gtdc1	A	C	2: 44,679,030 (GRCm39)		probably benign	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Igha	T	A	12: 113,222,684 (GRCm39)	N246Y		Het
Ighg2b	T	A	12: 113,270,926 (GRCm39)	T104S	probably benign	Het
Ighmbp2	C	T	19: 3,318,726 (GRCm39)	W450*	probably null	Het
Ipo11	T	C	13: 106,978,690 (GRCm39)	E839G	possibly damaging	Het
Iqce	T	C	5: 140,675,578 (GRCm39)	D207G	probably damaging	Het
Irgm2	T	C	11: 58,110,408 (GRCm39)	V45A	possibly damaging	Het
Itga1	C	T	13: 115,107,027 (GRCm39)	W1021*	probably null	Het
Itga3	T	C	11: 94,944,911 (GRCm39)	Y799C	probably damaging	Het
Klf1	T	C	8: 85,629,912 (GRCm39)	S246P	possibly damaging	Het
Lacc1	C	T	14: 77,272,613 (GRCm39)	C61Y	probably benign	Het
Lect2	T	A	13: 56,690,822 (GRCm39)		probably benign	Het
Leng9	G	A	7: 4,152,782 (GRCm39)		probably benign	Het
Lhx9	A	G	1: 138,769,704 (GRCm39)	C6R	probably damaging	Het
Matn3	T	A	12: 9,002,172 (GRCm39)	I128K	probably damaging	Het
Ndst1	T	C	18: 60,830,196 (GRCm39)	E638G	probably benign	Het
Nell2	A	T	15: 95,139,424 (GRCm39)	C603S	probably damaging	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Oas1h	T	G	5: 121,000,657 (GRCm39)	F89C	probably damaging	Het
Or4d10c	A	T	19: 12,066,056 (GRCm39)	Y33*	probably null	Het
Or52l1	A	G	7: 104,829,638 (GRCm39)	V294A	probably damaging	Het
Or5b101	T	A	19: 13,004,827 (GRCm39)	S289C		Het
Or5d45	C	T	2: 88,153,975 (GRCm39)	G25S	probably damaging	Het
P2ry2	G	A	7: 100,647,228 (GRCm39)	T359I	probably damaging	Het
Pbxip1	T	A	3: 89,352,864 (GRCm39)	V170E	probably benign	Het
Pcdhb20	T	A	18: 37,639,146 (GRCm39)	N557K	probably damaging	Het
Pdilt	A	G	7: 119,099,611 (GRCm39)	V219A	possibly damaging	Het
Plec	G	T	15: 76,067,310 (GRCm39)	S1149*	probably null	Het
Pnkp	C	T	7: 44,507,617 (GRCm39)	T61I	possibly damaging	Het
Polr1f	G	T	12: 33,487,867 (GRCm39)	E261*	probably null	Het
Prss56	T	A	1: 87,115,749 (GRCm39)	V541D	probably damaging	Het
Rab40c	A	G	17: 26,138,407 (GRCm39)	V25A	probably damaging	Het
Rgs8	A	T	1: 153,546,567 (GRCm39)	H22L	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Slc1a2	T	C	2: 102,586,353 (GRCm39)	V319A	probably damaging	Het
Sp140l2	G	A	1: 85,224,671 (GRCm39)	S288L	possibly damaging	Het
Spata18	A	T	5: 73,828,572 (GRCm39)	I378F	probably damaging	Het
Speer1i	T	A	5: 11,092,799 (GRCm39)	Y79*	probably null	Het
Stag1	T	C	9: 100,762,854 (GRCm39)	V485A	probably damaging	Het
Tas2r104	T	A	6: 131,662,569 (GRCm39)	I47F	probably damaging	Het
Thumpd1	A	T	7: 119,317,471 (GRCm39)	H143Q	possibly damaging	Het
Tmem231	T	C	8: 112,640,697 (GRCm39)	Q272R	probably damaging	Het
Tnfaip2	A	G	12: 111,412,310 (GRCm39)	D237G	probably benign	Het
Tox3	T	C	8: 91,074,543 (GRCm39)	D12G	probably benign	Het
Vmn1r1	A	G	1: 181,985,309 (GRCm39)	S119P	probably damaging	Het
Xrn1	A	G	9: 95,870,999 (GRCm39)	E608G	probably benign	Het
Zfp395	T	C	14: 65,629,528 (GRCm39)	C281R	probably damaging	Het

Other mutations in Kras

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00808:Kras	APN	6	145,192,474 (GRCm39)	missense	probably damaging	1.00
IGL02929:Kras	APN	6	145,177,815 (GRCm39)	intron	probably benign
N/A - 293:Kras	UTSW	6	145,177,940 (GRCm39)	missense	probably benign	0.01
R1463:Kras	UTSW	6	145,170,787 (GRCm39)	intron	probably benign
R1518:Kras	UTSW	6	145,177,977 (GRCm39)	missense	probably benign	0.00
R1603:Kras	UTSW	6	145,170,871 (GRCm39)	nonsense	probably null
R1885:Kras	UTSW	6	145,177,843 (GRCm39)	missense	probably damaging	1.00
R5089:Kras	UTSW	6	145,170,869 (GRCm39)	missense	probably benign	0.00
R5133:Kras	UTSW	6	145,177,879 (GRCm39)	missense	probably benign	0.00
R7710:Kras	UTSW	6	145,166,354 (GRCm39)	missense	probably benign
R7876:Kras	UTSW	6	145,170,848 (GRCm39)	missense	probably benign
R8151:Kras	UTSW	6	145,166,360 (GRCm39)	small deletion	probably benign
R8944:Kras	UTSW	6	145,170,853 (GRCm39)	missense	probably benign
R9345:Kras	UTSW	6	145,192,442 (GRCm39)	missense	probably benign	0.00
Z1177:Kras	UTSW	6	145,192,498 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAGTTTAAGTCCAAAACCCAGGG -3'
(R):5'- TGTCATCTGAGCATGGCAAG -3'

Sequencing Primer
(F):5'- GTTTAAGTCCAAAACCCAGGGAATAC -3'
(R):5'- TGGCAAGCCATCGCATATTAACATG -3'

Posted On

2021-08-31