Mutagenetix > Incidental Mutation

Incidental Mutation 'R8953:Wee1'

681759

Institutional Source

Beutler Lab

Gene Symbol

Wee1

Ensembl Gene

ENSMUSG00000031016

Gene Name

WEE 1 homolog 1 (S. pombe)

Synonyms

Wee1A

MMRRC Submission

068789-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8953 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109721266-109742506 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109723691 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 202 (D202G)

Ref Sequence

ENSEMBL: ENSMUSP00000033326 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033326]

AlphaFold

P47810

Predicted Effect

probably benign
Transcript: ENSMUST00000033326
AA Change: D202G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000033326
Gene: ENSMUSG00000031016
AA Change: D202G

Domain	Start	End	E-Value	Type
low complexity region	62	112	N/A	INTRINSIC
Pfam:Pkinase_Tyr	298	566	1.9e-26	PFAM
Pfam:Pkinase	298	568	1.7e-61	PFAM
low complexity region	620	631	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (64/64)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930590J08Rik	T	C	6: 91,892,002 (GRCm39)	V88A	probably benign	Het
Abca17	C	A	17: 24,518,015 (GRCm39)	V765L	probably benign	Het
Atp2b2	T	A	6: 113,737,630 (GRCm39)	T915S	possibly damaging	Het
Cacna1s	C	T	1: 136,025,170 (GRCm39)	H992Y	possibly damaging	Het
Castor1	A	T	11: 4,171,249 (GRCm39)	I244F	probably benign	Het
Clpp	T	A	17: 57,298,373 (GRCm39)	M113K	probably damaging	Het
Cmklr2	A	G	1: 63,222,272 (GRCm39)	L321P	probably damaging	Het
Csn3	A	G	5: 88,077,809 (GRCm39)	Q105R	possibly damaging	Het
Dcaf1	G	A	9: 106,735,542 (GRCm39)	R830Q	possibly damaging	Het
Dnah17	A	T	11: 118,016,238 (GRCm39)		probably benign	Het
Etv4	A	G	11: 101,662,513 (GRCm39)		probably null	Het
Fos	A	G	12: 85,522,826 (GRCm39)	D246G	probably benign	Het
Foxo1	T	C	3: 52,253,675 (GRCm39)	S613P	probably damaging	Het
Gabra2	A	T	5: 71,163,525 (GRCm39)	C261S	probably damaging	Het
Garem1	A	T	18: 21,264,388 (GRCm39)		probably null	Het
Gmip	T	G	8: 70,269,427 (GRCm39)	L533R	probably damaging	Het
Havcr1	A	G	11: 46,647,006 (GRCm39)	T147A	possibly damaging	Het
Helz2	C	T	2: 180,874,884 (GRCm39)	R1870Q	probably damaging	Het
Itprid2	T	C	2: 79,490,839 (GRCm39)	Y986H	probably damaging	Het
Kcnk3	A	C	5: 30,779,382 (GRCm39)	K144T	probably damaging	Het
Lama5	A	G	2: 179,835,313 (GRCm39)	V1293A	probably damaging	Het
Man2b1	A	G	8: 85,818,539 (GRCm39)	I498V	probably benign	Het
Map6	T	C	7: 98,965,078 (GRCm39)	L289P	probably damaging	Het
Mapk7	G	T	11: 61,383,792 (GRCm39)	D43E	possibly damaging	Het
Medag	A	G	5: 149,350,765 (GRCm39)	Y194C	probably damaging	Het
Mertk	A	G	2: 128,620,716 (GRCm39)		probably benign	Het
Mier3	C	A	13: 111,842,587 (GRCm39)	T136K	probably benign	Het
Ndc1	T	A	4: 107,238,890 (GRCm39)	W242R	probably damaging	Het
Nlrp1b	A	G	11: 71,052,632 (GRCm39)	V932A	probably damaging	Het
Or2m12	T	A	16: 19,105,269 (GRCm39)	M75L	probably benign	Het
Or5p55	G	A	7: 107,567,251 (GRCm39)	V216I	probably benign	Het
Or5w15	A	T	2: 87,568,371 (GRCm39)	M99K	probably benign	Het
Or9a4	G	T	6: 40,548,676 (GRCm39)	A119S	possibly damaging	Het
Otub2	A	T	12: 103,370,585 (GRCm39)	D289V	probably damaging	Het
P2rx3	A	G	2: 84,853,842 (GRCm39)	V133A	possibly damaging	Het
Pde4a	C	A	9: 21,122,030 (GRCm39)	T585K	probably damaging	Het
Pik3c2a	A	T	7: 115,987,320 (GRCm39)	Y534N	probably benign	Het
Pip5kl1	A	G	2: 32,469,991 (GRCm39)	D232G	possibly damaging	Het
Pklr	C	T	3: 89,049,612 (GRCm39)	S234L	probably damaging	Het
Pkn1	T	C	8: 84,410,815 (GRCm39)	K271E	probably damaging	Het
Plch1	T	G	3: 63,639,126 (GRCm39)	H449P	possibly damaging	Het
Pparg	T	C	6: 115,418,507 (GRCm39)	L92P	possibly damaging	Het
Pramel51	A	G	12: 88,144,070 (GRCm39)	C248R	probably benign	Het
Ptgdr2	C	T	19: 10,917,786 (GRCm39)	T101I	probably benign	Het
Rad54l2	A	T	9: 106,570,461 (GRCm39)	H1286Q	probably benign	Het
Ralgapa1	A	G	12: 55,867,546 (GRCm39)	S3P	probably damaging	Het
Rictor	G	A	15: 6,823,928 (GRCm39)	V1598I	probably benign	Het
Rph3al	G	T	11: 75,797,401 (GRCm39)	C98*	probably null	Het
Rpn2	A	G	2: 157,163,746 (GRCm39)	T613A	probably damaging	Het
Rps20	T	A	4: 3,834,617 (GRCm39)	M82L	probably benign	Het
Rusf1	T	G	7: 127,872,678 (GRCm39)	Q423H	probably benign	Het
Sh3bp4	A	G	1: 89,072,159 (GRCm39)	I336V	probably damaging	Het
Slc6a20b	T	C	9: 123,438,254 (GRCm39)	N174S	probably damaging	Het
Spta1	A	G	1: 174,058,241 (GRCm39)	N1903S	probably benign	Het
Tacc2	T	C	7: 130,227,487 (GRCm39)	S1391P	probably benign	Het
Terb1	T	A	8: 105,195,431 (GRCm39)	D570V	probably damaging	Het
Trhde	T	A	10: 114,338,966 (GRCm39)	I615F	probably damaging	Het
Ttc13	T	C	8: 125,402,088 (GRCm39)	N556S	probably damaging	Het
Ttn	T	A	2: 76,568,228 (GRCm39)	D19228V	probably damaging	Het
Unc13c	T	A	9: 73,840,044 (GRCm39)	Y269F	probably benign	Het
Usp33	T	A	3: 152,080,420 (GRCm39)	V517E	possibly damaging	Het
Zfp318	T	A	17: 46,731,356 (GRCm39)	S1120T	probably benign	Het
Zfr2	C	A	10: 81,084,271 (GRCm39)	N650K	probably damaging	Het
Znfx1	G	A	2: 166,897,421 (GRCm39)	T501I	probably damaging	Het

Other mutations in Wee1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00693:Wee1	APN	7	109,734,060 (GRCm39)	splice site	probably null
IGL00981:Wee1	APN	7	109,738,876 (GRCm39)	missense	probably damaging	1.00
IGL01017:Wee1	APN	7	109,725,055 (GRCm39)	missense	possibly damaging	0.93
IGL01357:Wee1	APN	7	109,741,242 (GRCm39)	missense	probably benign	0.39
IGL01838:Wee1	APN	7	109,723,744 (GRCm39)	missense	probably benign	0.01
IGL01970:Wee1	APN	7	109,738,457 (GRCm39)	missense	probably damaging	1.00
IGL02396:Wee1	APN	7	109,741,300 (GRCm39)	missense	probably damaging	1.00
IGL02511:Wee1	APN	7	109,738,483 (GRCm39)	missense	possibly damaging	0.55
IGL02884:Wee1	APN	7	109,725,269 (GRCm39)	missense	probably benign	0.02
IGL03085:Wee1	APN	7	109,723,805 (GRCm39)	missense	probably damaging	1.00
IGL03221:Wee1	APN	7	109,726,024 (GRCm39)	missense	probably damaging	1.00
IGL03383:Wee1	APN	7	109,738,899 (GRCm39)	missense	probably damaging	1.00
R0220:Wee1	UTSW	7	109,723,733 (GRCm39)	missense	probably benign	0.10
R1934:Wee1	UTSW	7	109,721,698 (GRCm39)	missense	probably benign	0.06
R3110:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3112:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3978:Wee1	UTSW	7	109,723,762 (GRCm39)	missense	probably damaging	1.00
R4348:Wee1	UTSW	7	109,730,165 (GRCm39)	missense	probably damaging	1.00
R5434:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5435:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5436:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5449:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5566:Wee1	UTSW	7	109,725,257 (GRCm39)	nonsense	probably null
R5630:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5632:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5685:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5694:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5807:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5941:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R6044:Wee1	UTSW	7	109,738,513 (GRCm39)	missense	probably benign	0.00
R6163:Wee1	UTSW	7	109,734,858 (GRCm39)	missense	probably damaging	1.00
R6826:Wee1	UTSW	7	109,723,870 (GRCm39)	critical splice donor site	probably null
R7203:Wee1	UTSW	7	109,734,001 (GRCm39)	missense	probably benign	0.00
R7835:Wee1	UTSW	7	109,730,085 (GRCm39)	nonsense	probably null
R8273:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R9077:Wee1	UTSW	7	109,725,963 (GRCm39)	missense	probably damaging	1.00
R9336:Wee1	UTSW	7	109,721,689 (GRCm39)	missense	probably damaging	1.00
R9463:Wee1	UTSW	7	109,721,917 (GRCm39)	missense	probably damaging	1.00
R9673:Wee1	UTSW	7	109,725,210 (GRCm39)	missense	probably damaging	0.98
R9748:Wee1	UTSW	7	109,721,722 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTAAGGCTTGTGTGTTGCC -3'
(R):5'- ACCTTAGGTGTGCAGAGGAACC -3'

Sequencing Primer
(F):5'- TCGGGTAACGGAACCTCTC -3'
(R):5'- TGTGCAGAGGAACCAAACTATAC -3'

Posted On

2021-08-31