Incidental Mutation 'R8962:Wisp2'
ID 682372
Institutional Source Beutler Lab
Gene Symbol Wisp2
Ensembl Gene ENSMUSG00000027656
Gene Name WNT1 inducible signaling pathway protein 2
Synonyms rCop1, Crgr4, CCN5
MMRRC Submission 068796-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8962 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 163820861-163833146 bp(+) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) C to T at 163825240 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Arginine to Stop codon at position 54 (R54*)
Ref Sequence ENSEMBL: ENSMUSP00000029188 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029188]
AlphaFold no structure available at present
Predicted Effect probably null
Transcript: ENSMUST00000029188
AA Change: R54*
SMART Domains Protein: ENSMUSP00000029188
Gene: ENSMUSG00000027656
AA Change: R54*

signal peptide 1 23 N/A INTRINSIC
IB 24 93 1.67e-16 SMART
VWC 100 163 5.9e-16 SMART
TSP1 195 239 9.68e-3 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.3%
  • 20x: 97.4%
Validation Efficiency 99% (86/87)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are viabe and overtly normal with no adult bone phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 86 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4931429L15Rik T A 9: 46,308,875 (GRCm38) M120L probably benign Het
6430573F11Rik A G 8: 36,505,575 (GRCm38) K60E probably damaging Het
Abat A G 16: 8,578,302 (GRCm38) T48A probably damaging Het
Abca8a A T 11: 110,078,808 (GRCm38) I314N probably damaging Het
Abr A T 11: 76,461,329 (GRCm38) V108D probably damaging Het
Adamts17 T G 7: 67,075,309 (GRCm38) L162V probably damaging Het
Adamts6 A T 13: 104,297,391 (GRCm38) K109N probably damaging Het
Ago3 A G 4: 126,347,802 (GRCm38) F68S probably damaging Het
Aifm3 T C 16: 17,506,336 (GRCm38) probably null Het
Ankrd26 T C 6: 118,535,143 (GRCm38) E506G probably benign Het
Apaf1 A G 10: 91,067,204 (GRCm38) L185P probably damaging Het
Atp8b3 G T 10: 80,520,062 (GRCm38) T1272K probably benign Het
Cars2 A G 8: 11,537,304 (GRCm38) V193A probably benign Het
Ccr8 A T 9: 120,094,547 (GRCm38) I243F possibly damaging Het
Col12a1 A G 9: 79,631,619 (GRCm38) V2465A probably damaging Het
Ctbp1 T C 5: 33,259,272 (GRCm38) N127S probably benign Het
Ddx10 A G 9: 53,238,077 (GRCm38) S117P probably damaging Het
Dgkb T C 12: 38,139,495 (GRCm38) probably null Het
Dnah11 C T 12: 117,954,895 (GRCm38) D1530N probably damaging Het
Dnah11 T A 12: 117,952,538 (GRCm38) D3520V probably damaging Het
Dock5 T C 14: 67,757,191 (GRCm38) T1807A probably benign Het
Drd3 C T 16: 43,821,479 (GRCm38) T386I probably damaging Het
Dsg1b A G 18: 20,409,259 (GRCm38) N941S probably damaging Het
Enpp3 T C 10: 24,820,615 (GRCm38) T141A probably benign Het
Esyt1 A T 10: 128,520,697 (GRCm38) C360S possibly damaging Het
Ethe1 T A 7: 24,606,257 (GRCm38) V143D probably damaging Het
Fam170b A G 14: 32,835,379 (GRCm38) D57G probably benign Het
Fam53c T C 18: 34,768,176 (GRCm38) S49P probably damaging Het
Fbp1 G A 13: 62,875,253 (GRCm38) L77F probably benign Het
Gm10471 T G 5: 26,085,747 (GRCm38) E142A probably benign Het
Gm17093 A G 14: 44,520,692 (GRCm38) E110G Het
Gm21976 G T 13: 98,287,313 (GRCm38) silent Het
Golgb1 T C 16: 36,913,616 (GRCm38) I1075T probably damaging Het
Grip2 T C 6: 91,777,410 (GRCm38) D628G probably damaging Het
Gtpbp1 G T 15: 79,717,728 (GRCm38) L557F probably benign Het
Ighv12-3 A T 12: 114,366,584 (GRCm38) F97Y probably benign Het
Itga8 T C 2: 12,191,234 (GRCm38) H635R possibly damaging Het
Itgb7 A G 15: 102,218,602 (GRCm38) L466S probably damaging Het
Klc2 T C 19: 5,111,836 (GRCm38) D277G probably benign Het
Ktn1 A G 14: 47,663,791 (GRCm38) E2G probably damaging Het
Lcmt1 T A 7: 123,401,446 (GRCm38) Y68N probably damaging Het
March10 G T 11: 105,389,989 (GRCm38) S490* probably null Het
Mterf1b A T 5: 4,196,437 (GRCm38) Y26F probably benign Het
Myo18b T A 5: 112,858,480 (GRCm38) I855F probably benign Het
Nek4 G A 14: 30,953,958 (GRCm38) M83I probably damaging Het
Nicn1 C T 9: 108,294,509 (GRCm38) R163C possibly damaging Het
Nos1 T C 5: 117,879,340 (GRCm38) V256A probably benign Het
Nr1h2 T C 7: 44,552,039 (GRCm38) T50A probably benign Het
Olfr296-ps1 T A 7: 86,562,109 (GRCm38) *126K probably null Het
Olfr732 A C 14: 50,281,359 (GRCm38) M298R possibly damaging Het
Ovol2 G C 2: 144,305,914 (GRCm38) R172G probably damaging Het
Parp14 A G 16: 35,856,817 (GRCm38) I927T probably damaging Het
Pcdhga7 T A 18: 37,715,826 (GRCm38) H295Q probably benign Het
Pkhd1l1 C T 15: 44,536,895 (GRCm38) T2145I probably damaging Het
Plekhs1 C T 19: 56,473,248 (GRCm38) T139I possibly damaging Het
Rab11fip3 T A 17: 26,012,033 (GRCm38) D746V probably damaging Het
Rnf214 A C 9: 45,898,430 (GRCm38) probably null Het
Rtn4ip1 A T 10: 43,946,419 (GRCm38) probably null Het
S1pr1 T C 3: 115,711,920 (GRCm38) R342G Het
Serpina1f T C 12: 103,689,872 (GRCm38) S366G probably benign Het
Sestd1 A T 2: 77,212,364 (GRCm38) M282K probably benign Het
Siglecf T G 7: 43,351,716 (GRCm38) V36G probably damaging Het
Slc1a1 T C 19: 28,909,469 (GRCm38) V410A probably damaging Het
Slc38a4 C A 15: 97,019,803 (GRCm38) D14Y probably benign Het
Slk C T 19: 47,622,309 (GRCm38) T806M probably damaging Het
Slurp2 C A 15: 74,743,400 (GRCm38) V48F possibly damaging Het
Socs1 A G 16: 10,784,778 (GRCm38) S32P possibly damaging Het
Son T C 16: 91,658,169 (GRCm38) V1268A possibly damaging Het
Sp7 C A 15: 102,366,445 (GRCm38) probably benign Het
Taar7b T G 10: 24,000,461 (GRCm38) S175A probably benign Het
Tet2 T C 3: 133,488,043 (GRCm38) N210S probably benign Het
Tkfc T C 19: 10,593,336 (GRCm38) D492G probably damaging Het
Tmc7 G A 7: 118,561,005 (GRCm38) P203L probably benign Het
Tshz2 C A 2: 169,884,604 (GRCm38) F373L probably damaging Het
Ttc29 T A 8: 78,315,707 (GRCm38) L307Q probably damaging Het
Tubd1 G A 11: 86,548,833 (GRCm38) M1I probably null Het
Vmn1r62 T A 7: 5,675,602 (GRCm38) M94K probably damaging Het
Vmn2r125 T C 4: 156,350,891 (GRCm38) I188T possibly damaging Het
Vmn2r5 T C 3: 64,491,143 (GRCm38) D805G probably damaging Het
Vmn2r6 A T 3: 64,556,155 (GRCm38) D419E probably damaging Het
Vps39 A T 2: 120,344,206 (GRCm38) Y98* probably null Het
Zdbf2 G A 1: 63,308,003 (GRCm38) G1847D probably benign Het
Zdhhc6 T C 19: 55,298,807 (GRCm38) E407G probably benign Het
Zfp407 A G 18: 84,558,932 (GRCm38) I1352T probably damaging Het
Zfp607a A G 7: 27,879,361 (GRCm38) K619E possibly damaging Het
Zfp811 A T 17: 32,798,648 (GRCm38) N139K probably benign Het
Other mutations in Wisp2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01447:Wisp2 APN 2 163,829,022 (GRCm38) missense probably damaging 1.00
BB002:Wisp2 UTSW 2 163,829,041 (GRCm38) missense possibly damaging 0.82
BB012:Wisp2 UTSW 2 163,829,041 (GRCm38) missense possibly damaging 0.82
R0336:Wisp2 UTSW 2 163,832,322 (GRCm38) missense probably damaging 0.98
R0600:Wisp2 UTSW 2 163,825,313 (GRCm38) missense probably damaging 1.00
R1241:Wisp2 UTSW 2 163,829,077 (GRCm38) missense unknown
R1779:Wisp2 UTSW 2 163,828,986 (GRCm38) missense probably damaging 1.00
R2921:Wisp2 UTSW 2 163,832,346 (GRCm38) missense probably benign 0.11
R2923:Wisp2 UTSW 2 163,832,346 (GRCm38) missense probably benign 0.11
R4049:Wisp2 UTSW 2 163,828,984 (GRCm38) missense probably damaging 1.00
R4344:Wisp2 UTSW 2 163,828,986 (GRCm38) missense probably damaging 1.00
R5409:Wisp2 UTSW 2 163,825,238 (GRCm38) missense probably damaging 1.00
R5529:Wisp2 UTSW 2 163,825,359 (GRCm38) critical splice donor site probably null
R5663:Wisp2 UTSW 2 163,825,253 (GRCm38) missense probably damaging 1.00
R6401:Wisp2 UTSW 2 163,829,026 (GRCm38) missense probably benign 0.45
R6685:Wisp2 UTSW 2 163,828,948 (GRCm38) missense possibly damaging 0.87
R7242:Wisp2 UTSW 2 163,828,852 (GRCm38) missense probably benign 0.27
R7925:Wisp2 UTSW 2 163,829,041 (GRCm38) missense possibly damaging 0.82
R8066:Wisp2 UTSW 2 163,828,942 (GRCm38) missense probably damaging 1.00
R8701:Wisp2 UTSW 2 163,828,866 (GRCm38) missense probably damaging 1.00
R9215:Wisp2 UTSW 2 163,829,046 (GRCm38) missense probably damaging 1.00
R9656:Wisp2 UTSW 2 163,829,065 (GRCm38) missense probably benign 0.04
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2021-08-31