Mutagenetix > Incidental Mutation

Incidental Mutation 'R8963:Helz2'

682468

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

068797-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8963 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

180869408-180883820 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 180871407 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 2735 (V2735E)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094203
AA Change: V2779E

PolyPhen 2 Score 0.624 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: V2779E

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000108831
AA Change: V2779E

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: V2779E

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000121484
AA Change: V2735E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: V2735E

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	T	C	11: 119,902,963 (GRCm39)	T421A	probably damaging	Het
Adamts12	T	C	15: 11,317,443 (GRCm39)		probably null	Het
Adgrv1	A	T	13: 81,567,588 (GRCm39)	V5195E	probably benign	Het
Adora2b	C	T	11: 62,139,983 (GRCm39)	A19V	possibly damaging	Het
Ankrd28	A	G	14: 31,477,698 (GRCm39)	C115R	probably benign	Het
Ankrd35	T	A	3: 96,587,003 (GRCm39)	L106*	probably null	Het
Ankrd44	G	A	1: 54,801,538 (GRCm39)	A263V	probably damaging	Het
Apol7b	T	C	15: 77,308,120 (GRCm39)	K125R	possibly damaging	Het
Cacna1c	A	T	6: 118,719,232 (GRCm39)	L245*	probably null	Het
Cbll1	A	T	12: 31,538,199 (GRCm39)	H185Q	probably damaging	Het
Ccdc150	A	C	1: 54,311,641 (GRCm39)	N209T	probably benign	Het
Ccdc88a	T	A	11: 29,448,416 (GRCm39)	N1465K	possibly damaging	Het
Cfap54	C	A	10: 92,864,562 (GRCm39)	G124*	probably null	Het
Chml	A	T	1: 175,514,601 (GRCm39)	L440H	probably damaging	Het
Chrnd	A	T	1: 87,122,603 (GRCm39)	Q128L	probably damaging	Het
Clvs2	A	T	10: 33,498,677 (GRCm39)	D84E	probably benign	Het
Ctif	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	18: 75,604,874 (GRCm39)		probably benign	Het
Cyp2c40	T	A	19: 39,755,926 (GRCm39)	I463F	possibly damaging	Het
Cyp51	C	A	5: 4,136,519 (GRCm39)	R425L	probably damaging	Het
Daam1	A	G	12: 71,992,018 (GRCm39)	T279A	unknown	Het
Ebf2	G	T	14: 67,665,554 (GRCm39)	V571F	probably benign	Het
Elapor2	T	A	5: 9,487,792 (GRCm39)	N559K	probably damaging	Het
Elavl4	A	T	4: 110,063,776 (GRCm39)	I275N	probably damaging	Het
F11r	A	G	1: 171,288,505 (GRCm39)	Q116R	probably benign	Het
Foxred2	T	A	15: 77,829,805 (GRCm39)	D580V	probably benign	Het
Gatad1	A	C	5: 3,691,544 (GRCm39)	L4R	probably damaging	Het
Gcn1	T	A	5: 115,727,153 (GRCm39)	M670K	probably benign	Het
Greb1	A	T	12: 16,774,885 (GRCm39)	F171I	probably damaging	Het
Grin2b	A	G	6: 136,021,007 (GRCm39)	V98A	probably damaging	Het
Hrct1	A	T	4: 43,727,564 (GRCm39)		probably benign	Het
Ighv1-11	C	T	12: 114,575,864 (GRCm39)	R117K	probably damaging	Het
Igkv12-46	A	T	6: 69,741,754 (GRCm39)	S34T	probably damaging	Het
Il27ra	T	A	8: 84,767,711 (GRCm39)	N71Y	probably damaging	Het
Iqca1	G	T	1: 90,067,649 (GRCm39)	H201N	probably benign	Het
Itgb1bp1	C	T	12: 21,324,864 (GRCm39)	R64Q	probably damaging	Het
Katnb1	T	C	8: 95,809,519 (GRCm39)	L13S	probably damaging	Het
Kif26b	G	A	1: 178,743,714 (GRCm39)	R1270Q	probably benign	Het
Klhdc2	A	T	12: 69,347,065 (GRCm39)	R77*	probably null	Het
Klra10	A	T	6: 130,249,617 (GRCm39)		probably null	Het
Large1	A	T	8: 73,542,612 (GRCm39)	I704N	probably damaging	Het
Lrp1b	G	A	2: 40,888,196 (GRCm39)	H2241Y	probably benign	Het
Lrpap1	T	A	5: 35,255,001 (GRCm39)	M212L	probably benign	Het
Mab21l1	A	G	3: 55,690,348 (GRCm39)		probably benign	Het
Map4k4	A	T	1: 40,039,740 (GRCm39)	Q44L	probably damaging	Het
Map7d1	A	G	4: 126,130,475 (GRCm39)	S412P	probably damaging	Het
Me1	A	T	9: 86,480,844 (GRCm39)	F354I	probably damaging	Het
Nlrp1b	A	G	11: 71,108,658 (GRCm39)	V281A	probably damaging	Het
Nrip2	A	G	6: 128,385,288 (GRCm39)	T240A	possibly damaging	Het
Nsmaf	A	G	4: 6,428,471 (GRCm39)	V203A	probably damaging	Het
Or12e8	T	A	2: 87,187,950 (GRCm39)	I54K	possibly damaging	Het
Or4g17	A	T	2: 111,209,645 (GRCm39)	Q100L	probably damaging	Het
Or4n5	A	T	14: 50,132,509 (GRCm39)	M250K	probably benign	Het
Or8g2	A	G	9: 39,821,495 (GRCm39)	Y132C	probably damaging	Het
P4ha2	T	C	11: 54,004,995 (GRCm39)	F124L	probably benign	Het
Pcdha9	A	T	18: 37,131,750 (GRCm39)	D273V	probably benign	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Plxnd1	G	T	6: 115,949,506 (GRCm39)	S760*	probably null	Het
Polr1b	A	G	2: 128,957,576 (GRCm39)	T544A	probably benign	Het
Polr2g	T	C	19: 8,771,513 (GRCm39)	D153G	probably damaging	Het
Pramel12	T	C	4: 143,144,229 (GRCm39)	Y192H	probably benign	Het
Rad51ap2	A	C	12: 11,506,255 (GRCm39)	E59A	possibly damaging	Het
Rrm1	A	T	7: 102,105,739 (GRCm39)	Y285F	probably benign	Het
Ryr3	A	G	2: 112,667,015 (GRCm39)		probably null	Het
Scaf4	GGCTGCTGCTGCTGCTGCTGCTGCTG	GGCTGCTGCTGCTGCTGCTGCTG	16: 90,026,745 (GRCm39)		probably benign	Het
Sftpd	A	G	14: 40,905,001 (GRCm39)	V30A	probably benign	Het
Sirt6	A	G	10: 81,462,378 (GRCm39)	V7A	probably benign	Het
Ska1	C	T	18: 74,330,639 (GRCm39)	V188M	probably damaging	Het
Skint8	G	A	4: 111,794,241 (GRCm39)	M210I	probably benign	Het
Slc6a11	A	G	6: 114,202,782 (GRCm39)		probably null	Het
Slc6a2	A	T	8: 93,715,702 (GRCm39)	H280L	probably benign	Het
Sptan1	T	C	2: 29,873,744 (GRCm39)	V208A	possibly damaging	Het
Taf6l	T	A	19: 8,752,135 (GRCm39)	T518S	probably benign	Het
Thoc2l	T	C	5: 104,665,652 (GRCm39)	V58A	probably benign	Het
Tsc22d1	T	C	14: 76,656,266 (GRCm39)	M59T	probably benign	Het
Unc5cl	A	G	17: 48,769,361 (GRCm39)	T282A	probably benign	Het
Vav3	A	G	3: 109,590,229 (GRCm39)	K260E	probably damaging	Het
Vmn2r53	A	G	7: 12,315,926 (GRCm39)	V631A	probably damaging	Het
Zfp938	A	T	10: 82,061,287 (GRCm39)	F444L	possibly damaging	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	180,871,495 (GRCm39)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	180,874,799 (GRCm39)	nonsense	probably null
IGL00704:Helz2	APN	2	180,876,178 (GRCm39)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	180,874,038 (GRCm39)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	180,875,770 (GRCm39)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	180,874,674 (GRCm39)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	180,880,274 (GRCm39)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	180,873,978 (GRCm39)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	180,873,483 (GRCm39)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	180,872,704 (GRCm39)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	180,872,815 (GRCm39)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	180,876,819 (GRCm39)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	180,872,939 (GRCm39)	intron	probably benign
IGL03003:Helz2	APN	2	180,882,046 (GRCm39)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	180,871,015 (GRCm39)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	180,873,597 (GRCm39)	missense	probably benign	0.00
Colby	UTSW	2	180,874,995 (GRCm39)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	180,876,627 (GRCm39)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	180,882,752 (GRCm39)	missense	probably benign
R0013:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	180,879,595 (GRCm39)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	180,874,062 (GRCm39)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	180,872,386 (GRCm39)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	180,874,002 (GRCm39)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	180,871,449 (GRCm39)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	180,869,563 (GRCm39)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	180,876,618 (GRCm39)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	180,873,882 (GRCm39)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	180,873,909 (GRCm39)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	180,882,674 (GRCm39)	missense	probably benign
R0826:Helz2	UTSW	2	180,882,646 (GRCm39)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	180,872,570 (GRCm39)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	180,877,928 (GRCm39)	missense	probably benign
R1170:Helz2	UTSW	2	180,871,608 (GRCm39)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	180,872,921 (GRCm39)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	180,879,389 (GRCm39)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	180,874,774 (GRCm39)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	180,877,317 (GRCm39)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	180,874,597 (GRCm39)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	180,875,021 (GRCm39)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	180,875,940 (GRCm39)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	180,878,056 (GRCm39)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	180,880,252 (GRCm39)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	180,876,780 (GRCm39)	missense	probably benign
R1837:Helz2	UTSW	2	180,871,082 (GRCm39)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	180,873,878 (GRCm39)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	180,876,082 (GRCm39)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	180,875,543 (GRCm39)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	180,873,122 (GRCm39)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	180,874,371 (GRCm39)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	180,879,272 (GRCm39)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	180,882,337 (GRCm39)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	180,876,895 (GRCm39)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	180,873,173 (GRCm39)	missense	probably benign
R2180:Helz2	UTSW	2	180,875,525 (GRCm39)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	180,870,841 (GRCm39)	nonsense	probably null
R2248:Helz2	UTSW	2	180,875,226 (GRCm39)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	180,874,705 (GRCm39)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	180,882,535 (GRCm39)	missense	probably benign
R3617:Helz2	UTSW	2	180,874,854 (GRCm39)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	180,882,182 (GRCm39)	nonsense	probably null
R3803:Helz2	UTSW	2	180,881,789 (GRCm39)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	180,871,503 (GRCm39)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	180,882,268 (GRCm39)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	180,871,695 (GRCm39)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	180,870,626 (GRCm39)	missense	probably benign
R4624:Helz2	UTSW	2	180,881,101 (GRCm39)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	180,880,210 (GRCm39)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	180,879,210 (GRCm39)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	180,871,913 (GRCm39)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	180,877,940 (GRCm39)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	180,874,231 (GRCm39)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	180,882,709 (GRCm39)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	180,882,362 (GRCm39)	missense	probably benign
R5089:Helz2	UTSW	2	180,876,942 (GRCm39)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	180,872,550 (GRCm39)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	180,876,639 (GRCm39)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	180,877,321 (GRCm39)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R5559:Helz2	UTSW	2	180,871,919 (GRCm39)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	180,882,051 (GRCm39)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	180,879,082 (GRCm39)	intron	probably benign
R5805:Helz2	UTSW	2	180,882,301 (GRCm39)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	180,878,189 (GRCm39)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	180,874,449 (GRCm39)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	180,875,821 (GRCm39)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	180,872,177 (GRCm39)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	180,872,560 (GRCm39)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	180,872,843 (GRCm39)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	180,882,106 (GRCm39)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	180,874,831 (GRCm39)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	180,874,087 (GRCm39)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	180,877,738 (GRCm39)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	180,874,995 (GRCm39)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R6581:Helz2	UTSW	2	180,871,172 (GRCm39)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	180,881,350 (GRCm39)	nonsense	probably null
R6964:Helz2	UTSW	2	180,872,221 (GRCm39)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	180,882,307 (GRCm39)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	180,873,078 (GRCm39)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	180,880,216 (GRCm39)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	180,877,393 (GRCm39)	splice site	probably null
R7512:Helz2	UTSW	2	180,872,647 (GRCm39)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	180,879,365 (GRCm39)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	180,873,789 (GRCm39)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	180,872,148 (GRCm39)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	180,876,324 (GRCm39)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	180,875,784 (GRCm39)	missense	probably benign
R7799:Helz2	UTSW	2	180,879,782 (GRCm39)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	180,874,695 (GRCm39)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	180,879,543 (GRCm39)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	180,881,998 (GRCm39)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	180,879,689 (GRCm39)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	180,880,055 (GRCm39)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	180,879,895 (GRCm39)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	180,871,950 (GRCm39)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	180,871,350 (GRCm39)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	180,874,560 (GRCm39)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	180,874,920 (GRCm39)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	180,880,173 (GRCm39)	missense
R8953:Helz2	UTSW	2	180,874,884 (GRCm39)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	180,879,581 (GRCm39)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	180,876,486 (GRCm39)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	180,870,792 (GRCm39)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	180,874,261 (GRCm39)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	180,881,968 (GRCm39)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	180,881,433 (GRCm39)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	180,881,848 (GRCm39)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	180,871,883 (GRCm39)	missense	probably benign
R9186:Helz2	UTSW	2	180,876,457 (GRCm39)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	180,882,741 (GRCm39)	missense	probably benign
R9407:Helz2	UTSW	2	180,881,975 (GRCm39)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	180,874,710 (GRCm39)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	180,878,245 (GRCm39)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	180,882,014 (GRCm39)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	180,882,470 (GRCm39)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	180,882,025 (GRCm39)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	180,882,616 (GRCm39)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	180,873,534 (GRCm39)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	180,879,357 (GRCm39)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	180,877,754 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTCTGAGCTCAGCAACCTC -3'
(R):5'- TCTTTGAGAGGTACCACAGGG -3'

Sequencing Primer
(F):5'- TGAGCTCAGCAACCTCACTCAATAC -3'
(R):5'- TGCTGGACACACAGTACCGTATG -3'

Posted On

2021-08-31