Incidental Mutation 'R8717:Adgrf3'
| ID |
682546 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Adgrf3
|
| Ensembl Gene |
ENSMUSG00000067642 |
| Gene Name |
adhesion G protein-coupled receptor F3 |
| Synonyms |
Gpr113, LOC381628, PGR23 |
| MMRRC Submission |
068570-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R8717 (G1)
|
| Quality Score |
206.009 |
|
Status
|
Validated
|
| Chromosome |
5 |
| Chromosomal Location |
30193431-30205722 bp(-) (GRCm38) |
| Type of Mutation |
unclassified |
| DNA Base Change (assembly) |
T to A
at 30198581 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000120958
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000088117]
[ENSMUST00000125367]
|
| AlphaFold |
Q58Y75 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000088117
|
| SMART Domains |
Protein: ENSMUSP00000085440
Gene: ENSMUSG00000067642
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
85 |
97 |
N/A |
INTRINSIC |
|
Blast:IG
|
163 |
252 |
2e-20 |
BLAST |
|
Blast:CCP
|
341 |
399 |
1e-6 |
BLAST |
|
low complexity region
|
403 |
415 |
N/A |
INTRINSIC |
|
low complexity region
|
471 |
483 |
N/A |
INTRINSIC |
|
GPS
|
632 |
684 |
2.68e-17 |
SMART |
|
Pfam:7tm_2
|
687 |
935 |
1e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000125367
|
| SMART Domains |
Protein: ENSMUSP00000120958
Gene: ENSMUSG00000067642
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
21 |
43 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.7%
- 20x: 98.9%
|
| Validation Efficiency |
100% (60/60) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 62 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 6030419C18Rik |
A |
G |
9: 58,499,340 (GRCm38) |
T178A |
probably damaging |
Het |
| A2ml1 |
A |
G |
6: 128,566,995 (GRCm38) |
F528L |
probably benign |
Het |
| Aadacl3 |
T |
G |
4: 144,456,208 (GRCm38) |
D230A |
probably damaging |
Het |
| Agtr1a |
A |
C |
13: 30,381,374 (GRCm38) |
T141P |
probably damaging |
Het |
| Ankfy1 |
T |
A |
11: 72,730,474 (GRCm38) |
D195E |
probably benign |
Het |
| AY074887 |
G |
A |
9: 54,950,502 (GRCm38) |
T113I |
probably damaging |
Het |
| Cacna1c |
G |
A |
6: 119,057,353 (GRCm38) |
A71V |
|
Het |
| Ccdc88b |
T |
C |
19: 6,855,845 (GRCm38) |
E278G |
probably damaging |
Het |
| Cntrl |
T |
A |
2: 35,113,339 (GRCm38) |
V78E |
probably benign |
Het |
| Cyp2c67 |
T |
A |
19: 39,638,711 (GRCm38) |
Y225F |
probably benign |
Het |
| Cyp2j13 |
T |
A |
4: 96,045,540 (GRCm38) |
H369L |
probably benign |
Het |
| Dagla |
T |
C |
19: 10,248,223 (GRCm38) |
E859G |
probably benign |
Het |
| Duox1 |
C |
A |
2: 122,337,671 (GRCm38) |
S1092R |
possibly damaging |
Het |
| Elfn2 |
C |
A |
15: 78,672,361 (GRCm38) |
S662I |
probably benign |
Het |
| Epor |
A |
T |
9: 21,959,445 (GRCm38) |
C380S |
probably benign |
Het |
| Fam213a |
G |
A |
14: 40,998,879 (GRCm38) |
P126S |
possibly damaging |
Het |
| Fsd2 |
T |
C |
7: 81,540,342 (GRCm38) |
E564G |
probably benign |
Het |
| Hace1 |
C |
T |
10: 45,605,598 (GRCm38) |
T117I |
unknown |
Het |
| Ift172 |
A |
G |
5: 31,255,641 (GRCm38) |
M1488T |
probably benign |
Het |
| Kdm3b |
A |
G |
18: 34,819,787 (GRCm38) |
E1070G |
probably damaging |
Het |
| Lrp6 |
A |
G |
6: 134,457,748 (GRCm38) |
F1331L |
probably benign |
Het |
| Lrrc74a |
G |
A |
12: 86,736,479 (GRCm38) |
E33K |
probably damaging |
Het |
| Magel2 |
T |
C |
7: 62,377,672 (GRCm38) |
M108T |
unknown |
Het |
| Mief1 |
T |
C |
15: 80,248,383 (GRCm38) |
S127P |
probably benign |
Het |
| Mprip |
T |
C |
11: 59,759,700 (GRCm38) |
V1410A |
probably benign |
Het |
| Muc1 |
G |
A |
3: 89,231,514 (GRCm38) |
V477M |
possibly damaging |
Het |
| Nat8f3 |
A |
T |
6: 85,761,705 (GRCm38) |
M25K |
possibly damaging |
Het |
| Neb |
T |
G |
2: 52,183,769 (GRCm38) |
H5942P |
probably damaging |
Het |
| Nf2 |
A |
T |
11: 4,816,099 (GRCm38) |
Y144N |
probably damaging |
Het |
| Nipbl |
T |
A |
15: 8,338,741 (GRCm38) |
M1166L |
probably benign |
Het |
| Npnt |
A |
T |
3: 132,908,375 (GRCm38) |
L206Q |
probably damaging |
Het |
| Olfr1288 |
A |
G |
2: 111,479,647 (GRCm38) |
T288A |
probably damaging |
Het |
| Olfr1337 |
T |
C |
4: 118,781,799 (GRCm38) |
Y262C |
probably damaging |
Het |
| Olfr808 |
T |
A |
10: 129,768,258 (GRCm38) |
I254N |
probably damaging |
Het |
| Parg |
A |
G |
14: 32,210,535 (GRCm38) |
K444R |
probably benign |
Het |
| Pcbp4 |
A |
T |
9: 106,460,003 (GRCm38) |
|
probably null |
Het |
| Pcdhgb4 |
A |
G |
18: 37,720,794 (GRCm38) |
S81G |
probably benign |
Het |
| Plat |
G |
T |
8: 22,772,232 (GRCm38) |
G91W |
probably damaging |
Het |
| Plekho2 |
T |
C |
9: 65,556,776 (GRCm38) |
N264D |
probably benign |
Het |
| Pmpca |
T |
A |
2: 26,391,881 (GRCm38) |
M182K |
probably damaging |
Het |
| Prdm10 |
A |
G |
9: 31,341,399 (GRCm38) |
H476R |
probably benign |
Het |
| Rab7 |
T |
C |
6: 88,012,387 (GRCm38) |
S34G |
probably damaging |
Het |
| Rbp3 |
G |
A |
14: 33,956,438 (GRCm38) |
R781H |
probably damaging |
Het |
| Rngtt |
T |
A |
4: 33,368,695 (GRCm38) |
S409R |
probably damaging |
Het |
| Ryr1 |
C |
A |
7: 29,052,328 (GRCm38) |
R3680L |
probably benign |
Het |
| Sbno1 |
T |
C |
5: 124,374,555 (GRCm38) |
S1384G |
possibly damaging |
Het |
| Shmt1 |
T |
C |
11: 60,794,937 (GRCm38) |
D271G |
probably benign |
Het |
| Sin3a |
C |
T |
9: 57,127,226 (GRCm38) |
R1228W |
probably damaging |
Het |
| Slc25a39 |
T |
C |
11: 102,403,794 (GRCm38) |
R294G |
probably benign |
Het |
| Smcr8 |
T |
A |
11: 60,779,428 (GRCm38) |
S467R |
probably damaging |
Het |
| Spaca7 |
A |
G |
8: 12,586,480 (GRCm38) |
Y102C |
probably damaging |
Het |
| Tacr1 |
A |
G |
6: 82,403,725 (GRCm38) |
Y39C |
probably damaging |
Het |
| Tenm3 |
G |
A |
8: 48,299,645 (GRCm38) |
S894L |
possibly damaging |
Het |
| Tmem115 |
T |
A |
9: 107,537,933 (GRCm38) |
M307K |
possibly damaging |
Het |
| Tnn |
A |
G |
1: 160,116,276 (GRCm38) |
V989A |
possibly damaging |
Het |
| Trabd2b |
T |
A |
4: 114,602,989 (GRCm38) |
N441K |
probably damaging |
Het |
| Ttll1 |
T |
C |
15: 83,489,508 (GRCm38) |
K368R |
probably benign |
Het |
| Tubgcp2 |
C |
T |
7: 139,996,792 (GRCm38) |
V858I |
probably benign |
Het |
| Ufsp2 |
G |
T |
8: 45,983,577 (GRCm38) |
V122L |
probably benign |
Het |
| Wdr26 |
A |
G |
1: 181,184,348 (GRCm38) |
C470R |
possibly damaging |
Het |
| Xab2 |
T |
C |
8: 3,613,845 (GRCm38) |
H358R |
probably benign |
Het |
| Xrcc5 |
A |
G |
1: 72,383,746 (GRCm38) |
I697V |
probably benign |
Het |
|
| Other mutations in Adgrf3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL03080:Adgrf3
|
APN |
5 |
30,196,829 (GRCm38) |
missense |
probably benign |
0.02 |
|
IGL03171:Adgrf3
|
APN |
5 |
30,196,294 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0010:Adgrf3
|
UTSW |
5 |
30,205,609 (GRCm38) |
splice site |
probably benign |
|
|
R0042:Adgrf3
|
UTSW |
5 |
30,197,428 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0140:Adgrf3
|
UTSW |
5 |
30,196,381 (GRCm38) |
missense |
probably benign |
0.19 |
|
R0617:Adgrf3
|
UTSW |
5 |
30,195,080 (GRCm38) |
missense |
probably benign |
0.25 |
|
R0748:Adgrf3
|
UTSW |
5 |
30,196,876 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1291:Adgrf3
|
UTSW |
5 |
30,199,534 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R1330:Adgrf3
|
UTSW |
5 |
30,195,095 (GRCm38) |
missense |
probably benign |
0.24 |
|
R1468:Adgrf3
|
UTSW |
5 |
30,202,229 (GRCm38) |
splice site |
probably benign |
|
|
R1695:Adgrf3
|
UTSW |
5 |
30,203,555 (GRCm38) |
missense |
probably benign |
0.05 |
|
R1716:Adgrf3
|
UTSW |
5 |
30,197,551 (GRCm38) |
missense |
probably benign |
0.03 |
|
R1844:Adgrf3
|
UTSW |
5 |
30,199,213 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R1935:Adgrf3
|
UTSW |
5 |
30,202,306 (GRCm38) |
missense |
probably benign |
0.00 |
|
R1936:Adgrf3
|
UTSW |
5 |
30,202,306 (GRCm38) |
missense |
probably benign |
0.00 |
|
R2059:Adgrf3
|
UTSW |
5 |
30,199,491 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
R2656:Adgrf3
|
UTSW |
5 |
30,196,438 (GRCm38) |
missense |
possibly damaging |
0.96 |
|
R2913:Adgrf3
|
UTSW |
5 |
30,196,994 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2914:Adgrf3
|
UTSW |
5 |
30,196,994 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2987:Adgrf3
|
UTSW |
5 |
30,197,360 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3797:Adgrf3
|
UTSW |
5 |
30,196,823 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R3798:Adgrf3
|
UTSW |
5 |
30,196,823 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R3799:Adgrf3
|
UTSW |
5 |
30,196,823 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R3934:Adgrf3
|
UTSW |
5 |
30,200,434 (GRCm38) |
unclassified |
probably benign |
|
|
R4043:Adgrf3
|
UTSW |
5 |
30,204,362 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4080:Adgrf3
|
UTSW |
5 |
30,197,369 (GRCm38) |
nonsense |
probably null |
|
|
R4575:Adgrf3
|
UTSW |
5 |
30,202,257 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4754:Adgrf3
|
UTSW |
5 |
30,197,617 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R4819:Adgrf3
|
UTSW |
5 |
30,198,444 (GRCm38) |
missense |
possibly damaging |
0.66 |
|
R4893:Adgrf3
|
UTSW |
5 |
30,200,478 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4991:Adgrf3
|
UTSW |
5 |
30,199,148 (GRCm38) |
missense |
probably benign |
0.26 |
|
R5686:Adgrf3
|
UTSW |
5 |
30,197,306 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5965:Adgrf3
|
UTSW |
5 |
30,205,639 (GRCm38) |
missense |
probably benign |
0.00 |
|
R5997:Adgrf3
|
UTSW |
5 |
30,198,362 (GRCm38) |
critical splice donor site |
probably null |
|
|
R6103:Adgrf3
|
UTSW |
5 |
30,196,267 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6244:Adgrf3
|
UTSW |
5 |
30,197,533 (GRCm38) |
missense |
probably benign |
0.17 |
|
R6409:Adgrf3
|
UTSW |
5 |
30,197,314 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R6575:Adgrf3
|
UTSW |
5 |
30,196,524 (GRCm38) |
missense |
possibly damaging |
0.72 |
|
R6745:Adgrf3
|
UTSW |
5 |
30,203,603 (GRCm38) |
missense |
probably benign |
0.31 |
|
R6790:Adgrf3
|
UTSW |
5 |
30,196,387 (GRCm38) |
missense |
probably benign |
0.00 |
|
R6813:Adgrf3
|
UTSW |
5 |
30,197,521 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R7202:Adgrf3
|
UTSW |
5 |
30,204,380 (GRCm38) |
nonsense |
probably null |
|
|
R7250:Adgrf3
|
UTSW |
5 |
30,195,682 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7353:Adgrf3
|
UTSW |
5 |
30,198,497 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R7634:Adgrf3
|
UTSW |
5 |
30,202,247 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7658:Adgrf3
|
UTSW |
5 |
30,197,206 (GRCm38) |
missense |
probably benign |
0.41 |
|
R8037:Adgrf3
|
UTSW |
5 |
30,199,512 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8281:Adgrf3
|
UTSW |
5 |
30,197,303 (GRCm38) |
missense |
possibly damaging |
0.46 |
|
R8857:Adgrf3
|
UTSW |
5 |
30,197,067 (GRCm38) |
nonsense |
probably null |
|
|
R8926:Adgrf3
|
UTSW |
5 |
30,200,448 (GRCm38) |
missense |
possibly damaging |
0.46 |
|
R9391:Adgrf3
|
UTSW |
5 |
30,195,073 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R9446:Adgrf3
|
UTSW |
5 |
30,196,959 (GRCm38) |
missense |
probably benign |
0.01 |
|
R9522:Adgrf3
|
UTSW |
5 |
30,199,484 (GRCm38) |
missense |
possibly damaging |
0.90 |
|
Z1088:Adgrf3
|
UTSW |
5 |
30,199,120 (GRCm38) |
missense |
possibly damaging |
0.90 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCAGTAAGGTCACAGTGTGC -3'
(R):5'- TGCTGGAGGTCTCTAGAAAGG -3'
Sequencing Primer
(F):5'- GTGCAGCATTTCCCATAAGTCAGAG -3'
(R):5'- GAGTGTGAAAGTGGATCCTCC -3'
|
| Posted On |
2021-09-07 |
Incidental Mutation