|Institutional Source||Beutler Lab|
|Gene Name||phospholipase C, gamma 1|
|Synonyms||Cded, Plc-gamma1, Plcg-1, Plc-1|
|Essential gene?||Essential (E-score: 1.000)|
|Stock #||R8708 (G1)|
|Chromosomal Location||160731300-160775760 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to A at 160754553 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000133771 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000103115] [ENSMUST00000109462] [ENSMUST00000151590]|
|Coding Region Coverage||
|Validation Efficiency||98% (99/101)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit early embryonic lethality associated with arrested growth and/or abnormal hematopoiesis. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Plcg1||
(F):5'- GATGCTGGGACTCCTAAGTTC -3'
(R):5'- GCCCTTCACAATGACAGGAC -3'
(F):5'- GGGACTCCTAAGTTCTTCTTGACAG -3'
(R):5'- GTCAGCAAGGAAGACAGCCC -3'