Mutagenetix > Incidental Mutation

Incidental Mutation 'R8968:Vmn2r14'

682844

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.420) question?

Stock #

R8968 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109215502-109224622 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109217667 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 514 (T514A)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably benign
Transcript: ENSMUST00000170341
AA Change: T514A

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: T514A

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	A	13: 77,332,310	L1153*	probably null	Het
2410089E03Rik	A	T	15: 8,201,281	H1014L	possibly damaging	Het
2810474O19Rik	T	A	6: 149,327,166	V570D	probably damaging	Het
Adamts2	T	C	11: 50,792,723	I944T	possibly damaging	Het
Aqp9	A	T	9: 71,138,203	C67*	probably null	Het
Armt1	G	T	10: 4,454,150	G412W	probably damaging	Het
B4galt1	T	C	4: 40,807,243	D381G	probably benign	Het
Bmp5	A	T	9: 75,873,297	H292L	probably benign	Het
Brpf1	T	C	6: 113,322,549	F1181S	probably damaging	Het
Btbd7	C	A	12: 102,812,766	G414C	probably damaging	Het
Ccdc65	A	T	15: 98,718,842	K204*	probably null	Het
Cdk5	T	A	5: 24,422,381	K88M	probably damaging	Het
Cdrt4	T	A	11: 62,992,808	L112Q	probably damaging	Het
Cenpb	T	C	2: 131,178,627	E417G	unknown	Het
Cep135	T	C	5: 76,606,729	I351T	possibly damaging	Het
Ces1d	A	T	8: 93,187,755	S226R	probably damaging	Het
Cfap97	T	A	8: 46,170,077	V168E	possibly damaging	Het
CN725425	T	C	15: 91,245,887	C318R	possibly damaging	Het
Cnga3	T	A	1: 37,261,379	D393E	probably benign	Het
Cpsf1	G	A	15: 76,601,969	R358*	probably null	Het
Cyp2b23	G	A	7: 26,679,538	P167L	probably damaging	Het
Cyp2c70	A	T	19: 40,153,615	H477Q	probably benign	Het
D930020B18Rik	A	C	10: 121,654,816	Y107S	probably damaging	Het
Dhrs1	A	T	14: 55,740,735	F216I	probably benign	Het
Dmgdh	A	T	13: 93,709,259	K474*	probably null	Het
Dsp	T	C	13: 38,151,620	I11T	possibly damaging	Het
Erh	G	T	12: 80,637,508	A65E	probably benign	Het
Fmnl1	AGGCTCTGG	AGG	11: 103,186,618		probably benign	Het
Grid2	A	C	6: 64,666,155	H967P	probably benign	Het
Hc	A	G	2: 35,032,305	I503T	possibly damaging	Het
Herc3	T	C	6: 58,890,198	L824S	probably damaging	Het
Hfm1	T	C	5: 106,917,573	D80G	probably benign	Het
Hjurp	CTCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCT	C	1: 88,266,277		probably benign	Het
Hspa1l	A	G	17: 34,977,254	K90E	possibly damaging	Het
Impa2	G	A	18: 67,318,427	V264I	probably benign	Het
Ipmk	C	T	10: 71,363,503	R65W	probably damaging	Het
Klhl38	C	T	15: 58,322,104	V410I	probably benign	Het
Lcn4	A	G	2: 26,668,348	I175T	possibly damaging	Het
Lipo2	C	A	19: 33,749,517	W40L	probably damaging	Het
Mgam	A	T	6: 40,757,811		probably null	Het
Myo16	T	A	8: 10,569,700	I1417N	unknown	Het
Olfr1052	G	A	2: 86,298,182	R122H	probably benign	Het
Olfr1196	T	A	2: 88,700,793	I179F	probably benign	Het
Olfr136	A	T	17: 38,335,429	T91S	possibly damaging	Het
Olfr603	A	T	7: 103,383,460	C181S	probably damaging	Het
Pcdha11	A	G	18: 37,012,254	N466S	probably damaging	Het
Pif1	C	T	9: 65,591,794	T432M	probably damaging	Het
Pigt	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	2: 164,499,669		probably null	Het
Pkd1l3	CCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGACACACCTGCATCCAATAGCCCACCACAGGGGACATCAGACACACCTGGATTCAGCAGCCCAACACAGGTGACAACAGCCACACTTGTATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACATCTGCATCCATCAGCCCACCACAGGTAATATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACA	CCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGACACACCTGCATCCAATAGCCCACCACAGGGGACATCAGACACACCTGGATTCAGCAGCCCAACACAGGTGACAACAGCCACACTTGTATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACATCTGCATCCATCAGCCCACCACAGGTAATATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACA	8: 109,623,788		probably benign	Het
Pole	T	C	5: 110,312,083	S1118P	possibly damaging	Het
Ppp4r4	T	C	12: 103,600,447	M652T	probably benign	Het
Prex2	A	T	1: 11,110,338	K376*	probably null	Het
Prps1l1	A	T	12: 34,985,206	I107F	probably damaging	Het
Rfc1	C	T	5: 65,275,435	V761I	probably benign	Het
Ripk4	T	C	16: 97,746,003	E353G	probably benign	Het
Robo2	C	A	16: 73,971,053		probably null	Het
Rrm1	C	T	7: 102,468,338	A745V	probably benign	Het
Sash1	A	T	10: 8,730,415	V737D	probably benign	Het
Sel1l2	T	C	2: 140,285,289	K101E	probably benign	Het
Selenbp2	A	T	3: 94,700,030	I253F	probably benign	Het
Setd1a	C	T	7: 127,786,107	P662L	possibly damaging	Het
Slc16a6	C	T	11: 109,454,950	V496I	possibly damaging	Het
Slc4a4	A	T	5: 89,084,653	M239L	probably benign	Het
Slc7a8	G	C	14: 54,781,293	A12G	probably benign	Het
Snx30	T	C	4: 59,886,517	S309P	possibly damaging	Het
Spen	T	C	4: 141,470,390	N3389S	probably benign	Het
Spg11	T	C	2: 122,092,206	T921A	probably damaging	Het
Tanc2	T	A	11: 105,867,574	D720E	possibly damaging	Het
Tmprss9	A	T	10: 80,894,329	I688F	possibly damaging	Het
Trap1	A	G	16: 4,044,626	V596A	possibly damaging	Het
Ttc32	G	T	12: 9,030,187	R44L	probably benign	Het
Tusc3	T	A	8: 39,130,744	N288K	probably benign	Het
Ush2a	G	A	1: 188,395,759	G656E	probably damaging	Het
Vill	T	C	9: 119,063,603		probably null	Het
Vmn1r5	A	T	6: 56,986,197	R286*	probably null	Het
Vmn2r32	G	A	7: 7,474,205	H396Y	probably benign	Het
Vmn2r75	A	T	7: 86,171,557	Y56*	probably null	Het
Wdfy3	C	T	5: 101,864,117	S2668N	probably benign	Het
Wdr72	G	A	9: 74,152,447	D392N	probably benign	Het
Zfp518a	A	C	19: 40,913,426	K600Q	possibly damaging	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109216314	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109221419	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109224577	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109220409	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109224483	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109220067	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109220588	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109220016	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109221439	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109220188	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109221426	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109216394	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109216107	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109220484	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109219836	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109219044	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109218896	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109216360	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109224574	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109216251	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109220329	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109220329	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109215996	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109221417	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109219972	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109219047	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109221243	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109218832	splice site	probably null
R2417:Vmn2r14	UTSW	5	109224463	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109215910	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109224565	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109216229	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109220167	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109216064	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109216064	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109216283	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109216283	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109216411	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109216411	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109221504	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109216110	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109221518	splice site	probably null
R4896:Vmn2r14	UTSW	5	109220380	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109220380	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109216095	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109217576	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109221288	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109220395	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109219950	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109217620	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109215858	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109221356	missense	probably benign
R5985:Vmn2r14	UTSW	5	109220216	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109221417	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109221267	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109216230	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109216059	missense	probably benign	0.22
R6944:Vmn2r14	UTSW	5	109216274	missense	probably benign	0.06
R7608:Vmn2r14	UTSW	5	109221410	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109220220	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109221353	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109215996	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109220554	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109221476	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109221474	missense	probably benign	0.30
R9008:Vmn2r14	UTSW	5	109220027	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109220188	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109220036	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109219917	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109216221	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109221246	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109221422	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109220310	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109220562	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109220096	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109216175	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109221260	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109219875	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- AGGTCTTCAGTTTCCATCTAGGCC -3'
(R):5'- CCTGATACTGTTAAGTAATGCTAGC -3'

Sequencing Primer
(F):5'- TCAGTTTCCATCTAGGCCATATC -3'
(R):5'- TGTTAAGTAATGCTAGCTATCAGAGG -3'

Posted On

2021-10-11