Mutagenetix > Incidental Mutation

Incidental Mutation 'R8968:Vmn2r32'

682852

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r32

Ensembl Gene

ENSMUSG00000096743

Gene Name

vomeronasal 2, receptor 32

Synonyms

V2r5

MMRRC Submission

068802-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.190) question?

Stock #

R8968 (G1)

Quality Score

184.009

Status

Not validated

Chromosome

Chromosomal Location

7463015-7479973 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 7474205 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Tyrosine at position 396 (H396Y)

Ref Sequence

ENSEMBL: ENSMUSP00000092462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094866]

AlphaFold

K7N686

Predicted Effect

probably benign
Transcript: ENSMUST00000094866
AA Change: H396Y

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000092462
Gene: ENSMUSG00000096743
AA Change: H396Y

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	3.6e-33	PFAM
Pfam:NCD3G	512	565	8.1e-21	PFAM
Pfam:7tm_3	598	833	9.5e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	A	13: 77,332,310 (GRCm38)	L1153*	probably null	Het
Adamts2	T	C	11: 50,792,723 (GRCm38)	I944T	possibly damaging	Het
Aqp9	A	T	9: 71,138,203 (GRCm38)	C67*	probably null	Het
Armt1	G	T	10: 4,454,150 (GRCm38)	G412W	probably damaging	Het
B4galt1	T	C	4: 40,807,243 (GRCm38)	D381G	probably benign	Het
Bmp5	A	T	9: 75,873,297 (GRCm38)	H292L	probably benign	Het
Brpf1	T	C	6: 113,322,549 (GRCm38)	F1181S	probably damaging	Het
Btbd7	C	A	12: 102,812,766 (GRCm38)	G414C	probably damaging	Het
Ccdc65	A	T	15: 98,718,842 (GRCm38)	K204*	probably null	Het
Cdk5	T	A	5: 24,422,381 (GRCm38)	K88M	probably damaging	Het
Cdrt4	T	A	11: 62,992,808 (GRCm38)	L112Q	probably damaging	Het
Cenpb	T	C	2: 131,178,627 (GRCm38)	E417G	unknown	Het
Cep135	T	C	5: 76,606,729 (GRCm38)	I351T	possibly damaging	Het
Ces1d	A	T	8: 93,187,755 (GRCm38)	S226R	probably damaging	Het
Cfap97	T	A	8: 46,170,077 (GRCm38)	V168E	possibly damaging	Het
CN725425	T	C	15: 91,245,887 (GRCm38)	C318R	possibly damaging	Het
Cnga3	T	A	1: 37,261,379 (GRCm38)	D393E	probably benign	Het
Cplane1	A	T	15: 8,201,281 (GRCm38)	H1014L	possibly damaging	Het
Cpsf1	G	A	15: 76,601,969 (GRCm38)	R358*	probably null	Het
Cyp2b23	G	A	7: 26,679,538 (GRCm38)	P167L	probably damaging	Het
Cyp2c70	A	T	19: 40,153,615 (GRCm38)	H477Q	probably benign	Het
D930020B18Rik	A	C	10: 121,654,816 (GRCm38)	Y107S	probably damaging	Het
Dhrs1	A	T	14: 55,740,735 (GRCm38)	F216I	probably benign	Het
Dmgdh	A	T	13: 93,709,259 (GRCm38)	K474*	probably null	Het
Dsp	T	C	13: 38,151,620 (GRCm38)	I11T	possibly damaging	Het
Erh	G	T	12: 80,637,508 (GRCm38)	A65E	probably benign	Het
Fmnl1	AGGCTCTGG	AGG	11: 103,186,618 (GRCm38)		probably benign	Het
Grid2	A	C	6: 64,666,155 (GRCm38)	H967P	probably benign	Het
Hc	A	G	2: 35,032,305 (GRCm38)	I503T	possibly damaging	Het
Herc3	T	C	6: 58,890,198 (GRCm38)	L824S	probably damaging	Het
Hfm1	T	C	5: 106,917,573 (GRCm38)	D80G	probably benign	Het
Hjurp	CTCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCT	C	1: 88,266,277 (GRCm38)		probably benign	Het
Hspa1l	A	G	17: 34,977,254 (GRCm38)	K90E	possibly damaging	Het
Impa2	G	A	18: 67,318,427 (GRCm38)	V264I	probably benign	Het
Ipmk	C	T	10: 71,363,503 (GRCm38)	R65W	probably damaging	Het
Klhl38	C	T	15: 58,322,104 (GRCm38)	V410I	probably benign	Het
Lcn4	A	G	2: 26,668,348 (GRCm38)	I175T	possibly damaging	Het
Lipo2	C	A	19: 33,749,517 (GRCm38)	W40L	probably damaging	Het
Mgam	A	T	6: 40,757,811 (GRCm38)		probably null	Het
Myo16	T	A	8: 10,569,700 (GRCm38)	I1417N	unknown	Het
Or2n1d	A	T	17: 38,335,429 (GRCm38)	T91S	possibly damaging	Het
Or4a66	T	A	2: 88,700,793 (GRCm38)	I179F	probably benign	Het
Or52e19b	A	T	7: 103,383,460 (GRCm38)	C181S	probably damaging	Het
Or5j3	G	A	2: 86,298,182 (GRCm38)	R122H	probably benign	Het
Pcdha11	A	G	18: 37,012,254 (GRCm38)	N466S	probably damaging	Het
Pif1	C	T	9: 65,591,794 (GRCm38)	T432M	probably damaging	Het
Pigt	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	2: 164,499,669 (GRCm38)		probably null	Het
Pkd1l3	CCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGACACACCTGCATCCAATAGCCCACCACAGGGGACATCAGACACACCTGGATTCAGCAGCCCAACACAGGTGACAACAGCCACACTTGTATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACATCTGCATCCATCAGCCCACCACAGGTAATATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACA	CCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGACACACCTGCATCCAATAGCCCACCACAGGGGACATCAGACACACCTGGATTCAGCAGCCCAACACAGGTGACAACAGCCACACTTGTATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACATCTGCATCCATCAGCCCACCACAGGTAATATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACA	8: 109,623,788 (GRCm38)		probably benign	Het
Pole	T	C	5: 110,312,083 (GRCm38)	S1118P	possibly damaging	Het
Ppp4r4	T	C	12: 103,600,447 (GRCm38)	M652T	probably benign	Het
Prex2	A	T	1: 11,110,338 (GRCm38)	K376*	probably null	Het
Prps1l1	A	T	12: 34,985,206 (GRCm38)	I107F	probably damaging	Het
Resf1	T	A	6: 149,327,166 (GRCm38)	V570D	probably damaging	Het
Rfc1	C	T	5: 65,275,435 (GRCm38)	V761I	probably benign	Het
Ripk4	T	C	16: 97,746,003 (GRCm38)	E353G	probably benign	Het
Robo2	C	A	16: 73,971,053 (GRCm38)		probably null	Het
Rrm1	C	T	7: 102,468,338 (GRCm38)	A745V	probably benign	Het
Sash1	A	T	10: 8,730,415 (GRCm38)	V737D	probably benign	Het
Sel1l2	T	C	2: 140,285,289 (GRCm38)	K101E	probably benign	Het
Selenbp2	A	T	3: 94,700,030 (GRCm38)	I253F	probably benign	Het
Setd1a	C	T	7: 127,786,107 (GRCm38)	P662L	possibly damaging	Het
Slc16a6	C	T	11: 109,454,950 (GRCm38)	V496I	possibly damaging	Het
Slc4a4	A	T	5: 89,084,653 (GRCm38)	M239L	probably benign	Het
Slc7a8	G	C	14: 54,781,293 (GRCm38)	A12G	probably benign	Het
Snx30	T	C	4: 59,886,517 (GRCm38)	S309P	possibly damaging	Het
Spen	T	C	4: 141,470,390 (GRCm38)	N3389S	probably benign	Het
Spg11	T	C	2: 122,092,206 (GRCm38)	T921A	probably damaging	Het
Tanc2	T	A	11: 105,867,574 (GRCm38)	D720E	possibly damaging	Het
Tmprss9	A	T	10: 80,894,329 (GRCm38)	I688F	possibly damaging	Het
Trap1	A	G	16: 4,044,626 (GRCm38)	V596A	possibly damaging	Het
Ttc32	G	T	12: 9,030,187 (GRCm38)	R44L	probably benign	Het
Tusc3	T	A	8: 39,130,744 (GRCm38)	N288K	probably benign	Het
Ush2a	G	A	1: 188,395,759 (GRCm38)	G656E	probably damaging	Het
Vill	T	C	9: 119,063,603 (GRCm38)		probably null	Het
Vmn1r5	A	T	6: 56,986,197 (GRCm38)	R286*	probably null	Het
Vmn2r14	T	C	5: 109,217,667 (GRCm38)	T514A	probably benign	Het
Vmn2r75	A	T	7: 86,171,557 (GRCm38)	Y56*	probably null	Het
Wdfy3	C	T	5: 101,864,117 (GRCm38)	S2668N	probably benign	Het
Wdr72	G	A	9: 74,152,447 (GRCm38)	D392N	probably benign	Het
Zfp518a	A	C	19: 40,913,426 (GRCm38)	K600Q	possibly damaging	Het

Other mutations in Vmn2r32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Vmn2r32	APN	7	7,476,697 (GRCm38)	missense	probably damaging	0.97
IGL02333:Vmn2r32	APN	7	7,464,144 (GRCm38)	missense	probably damaging	1.00
IGL02406:Vmn2r32	APN	7	7,476,710 (GRCm38)	missense	probably benign
IGL02428:Vmn2r32	APN	7	7,474,284 (GRCm38)	missense	probably benign	0.02
IGL02484:Vmn2r32	APN	7	7,464,117 (GRCm38)	missense	probably damaging	1.00
IGL03277:Vmn2r32	APN	7	7,474,252 (GRCm38)	missense	probably benign	0.23
IGL03366:Vmn2r32	APN	7	7,464,030 (GRCm38)	missense	probably damaging	0.99
R1055:Vmn2r32	UTSW	7	7,474,327 (GRCm38)	nonsense	probably null
R1695:Vmn2r32	UTSW	7	7,463,992 (GRCm38)	missense	probably benign	0.01
R2172:Vmn2r32	UTSW	7	7,474,615 (GRCm38)	missense	probably damaging	0.99
R2262:Vmn2r32	UTSW	7	7,474,619 (GRCm38)	missense	probably benign
R3150:Vmn2r32	UTSW	7	7,472,555 (GRCm38)	missense	probably benign
R4362:Vmn2r32	UTSW	7	7,479,858 (GRCm38)	nonsense	probably null
R4432:Vmn2r32	UTSW	7	7,479,919 (GRCm38)	missense	probably damaging	0.98
R4851:Vmn2r32	UTSW	7	7,479,954 (GRCm38)	missense	possibly damaging	0.59
R4949:Vmn2r32	UTSW	7	7,464,084 (GRCm38)	missense	probably benign	0.22
R5990:Vmn2r32	UTSW	7	7,479,810 (GRCm38)	missense	probably damaging	0.97
R6083:Vmn2r32	UTSW	7	7,464,210 (GRCm38)	missense	probably benign	0.15
R6084:Vmn2r32	UTSW	7	7,464,210 (GRCm38)	missense	probably benign	0.15
R6116:Vmn2r32	UTSW	7	7,464,093 (GRCm38)	missense	probably damaging	1.00
R6263:Vmn2r32	UTSW	7	7,476,692 (GRCm38)	missense	possibly damaging	0.90
R6889:Vmn2r32	UTSW	7	7,472,574 (GRCm38)	missense	possibly damaging	0.82
R7286:Vmn2r32	UTSW	7	7,479,808 (GRCm38)	missense	probably benign	0.21
R7390:Vmn2r32	UTSW	7	7,479,852 (GRCm38)	missense	probably benign	0.00
R7412:Vmn2r32	UTSW	7	7,474,213 (GRCm38)	missense	possibly damaging	0.53
R7508:Vmn2r32	UTSW	7	7,467,374 (GRCm38)	missense	possibly damaging	0.87
R8812:Vmn2r32	UTSW	7	7,474,670 (GRCm38)	missense	probably damaging	1.00
R9331:Vmn2r32	UTSW	7	7,464,403 (GRCm38)	nonsense	probably null
R9358:Vmn2r32	UTSW	7	7,474,198 (GRCm38)	missense	probably damaging	1.00
Z1177:Vmn2r32	UTSW	7	7,474,161 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGTCTGGGCATCTGAAAGAC -3'
(R):5'- AGTCATGACACATTCTATGGATCAC -3'

Sequencing Primer
(F):5'- GTCTGGGCATCTGAAAGACTCTAAAC -3'
(R):5'- ACCATGGTGAGATTTCTGGC -3'

Posted On

2021-10-11