Mutagenetix > Incidental Mutation

Incidental Mutation 'R8969:Ash1l'

682918

Institutional Source

Beutler Lab

Gene Symbol

Ash1l

Ensembl Gene

ENSMUSG00000028053

Gene Name

ASH1 like histone lysine methyltransferase

Synonyms

E430018P19Rik, 8030453L17Rik, KMT2H, chromatin remodeling factor

MMRRC Submission

068803-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8969 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

88857929-88986682 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88873598 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 127 (M127T)

Ref Sequence

ENSEMBL: ENSMUSP00000088451 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090933] [ENSMUST00000186583]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000090933
AA Change: M127T

PolyPhen 2 Score 0.523 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000088451
Gene: ENSMUSG00000028053
AA Change: M127T

Domain	Start	End	E-Value	Type
low complexity region	36	46	N/A	INTRINSIC
low complexity region	226	237	N/A	INTRINSIC
internal_repeat_1	238	306	6.88e-12	PROSPERO
internal_repeat_1	306	406	6.88e-12	PROSPERO
low complexity region	552	571	N/A	INTRINSIC
low complexity region	706	717	N/A	INTRINSIC
low complexity region	745	753	N/A	INTRINSIC
low complexity region	777	791	N/A	INTRINSIC
AT_hook	823	835	3.06e2	SMART
low complexity region	859	873	N/A	INTRINSIC
AT_hook	885	897	9.15e0	SMART
low complexity region	938	948	N/A	INTRINSIC
low complexity region	1086	1105	N/A	INTRINSIC
low complexity region	1107	1121	N/A	INTRINSIC
low complexity region	1159	1173	N/A	INTRINSIC
low complexity region	1262	1273	N/A	INTRINSIC
low complexity region	1288	1301	N/A	INTRINSIC
AT_hook	1345	1357	3.09e-1	SMART
low complexity region	1377	1388	N/A	INTRINSIC
low complexity region	1395	1424	N/A	INTRINSIC
low complexity region	1478	1491	N/A	INTRINSIC
low complexity region	1678	1692	N/A	INTRINSIC
AT_hook	1843	1855	1.03e1	SMART
low complexity region	1971	1983	N/A	INTRINSIC
AWS	2081	2133	3.95e-26	SMART
SET	2135	2257	8.04e-45	SMART
PostSET	2259	2275	6.38e-2	SMART
low complexity region	2296	2316	N/A	INTRINSIC
BROMO	2431	2541	8.29e-23	SMART
low complexity region	2549	2563	N/A	INTRINSIC
PHD	2576	2618	8.25e-6	SMART
BAH	2650	2787	1.18e-23	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000186583
AA Change: M127T

PolyPhen 2 Score 0.523 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000140251
Gene: ENSMUSG00000028053
AA Change: M127T

Domain	Start	End	E-Value	Type
low complexity region	36	46	N/A	INTRINSIC
low complexity region	226	237	N/A	INTRINSIC
internal_repeat_1	238	306	6.88e-12	PROSPERO
internal_repeat_1	306	406	6.88e-12	PROSPERO
low complexity region	552	571	N/A	INTRINSIC
low complexity region	706	717	N/A	INTRINSIC
low complexity region	745	753	N/A	INTRINSIC
low complexity region	777	791	N/A	INTRINSIC
AT_hook	823	835	3.06e2	SMART
low complexity region	859	873	N/A	INTRINSIC
AT_hook	885	897	9.15e0	SMART
low complexity region	938	948	N/A	INTRINSIC
low complexity region	1086	1105	N/A	INTRINSIC
low complexity region	1107	1121	N/A	INTRINSIC
low complexity region	1159	1173	N/A	INTRINSIC
low complexity region	1262	1273	N/A	INTRINSIC
low complexity region	1288	1301	N/A	INTRINSIC
AT_hook	1345	1357	3.09e-1	SMART
low complexity region	1377	1388	N/A	INTRINSIC
low complexity region	1395	1424	N/A	INTRINSIC
low complexity region	1478	1491	N/A	INTRINSIC
low complexity region	1678	1692	N/A	INTRINSIC
AT_hook	1843	1855	1.03e1	SMART
low complexity region	1971	1983	N/A	INTRINSIC
AWS	2081	2133	3.95e-26	SMART
SET	2135	2257	8.04e-45	SMART
PostSET	2259	2275	6.38e-2	SMART
low complexity region	2296	2316	N/A	INTRINSIC
BROMO	2431	2541	8.29e-23	SMART
low complexity region	2549	2563	N/A	INTRINSIC
PHD	2576	2618	8.25e-6	SMART
BAH	2650	2787	1.18e-23	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (77/77)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a transposon-induced allele are more susceptible to endotoxin shock, sepsis, and autoimmune disease. Homozygotes for a hypomorphic allele show reduced growth and postnatal lethality; surviving adults lack Meibomian glands and show vertebral, reproductive organ, and fertility defects. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Gene trapped(4)

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,227,944 (GRCm39)	N662K	probably benign	Het
Acp7	G	A	7: 28,307,382 (GRCm39)	R492W	probably damaging	Het
Adam33	C	T	2: 130,894,994 (GRCm39)	G688D	probably damaging	Het
Ak3	T	G	19: 29,025,094 (GRCm39)	M46L	probably benign	Het
Aldh1l1	A	G	6: 90,547,790 (GRCm39)	T417A	probably benign	Het
Ambp	C	T	4: 63,072,328 (GRCm39)		probably benign	Het
Arhgef11	T	C	3: 87,632,949 (GRCm39)	S687P	probably damaging	Het
Atg9b	C	T	5: 24,592,832 (GRCm39)	A524T	probably benign	Het
Btbd10	A	T	7: 112,925,162 (GRCm39)	S277T	probably damaging	Het
Ccdc167	T	C	17: 29,924,553 (GRCm39)	D16G	probably damaging	Het
Cdh12	A	G	15: 21,492,739 (GRCm39)	T253A	probably damaging	Het
Cfap206	A	T	4: 34,692,522 (GRCm39)	D501E	probably benign	Het
Chrnb2	A	G	3: 89,664,532 (GRCm39)	F461L	probably damaging	Het
Clic1	T	C	17: 35,274,386 (GRCm39)		probably null	Het
Cmah	A	G	13: 24,606,636 (GRCm39)	Y89C	probably damaging	Het
Crkl	A	G	16: 17,286,918 (GRCm39)	E158G	probably damaging	Het
Cyp3a59	A	T	5: 146,049,630 (GRCm39)	E486V	probably benign	Het
D16Ertd472e	T	G	16: 78,344,682 (GRCm39)	Y142S	probably damaging	Het
Decr2	T	A	17: 26,306,355 (GRCm39)	M94L	probably benign	Het
Dspp	A	T	5: 104,325,640 (GRCm39)	S668C	unknown	Het
Dync2h1	T	C	9: 7,130,723 (GRCm39)	R1708G	probably damaging	Het
Eno3	T	A	11: 70,551,691 (GRCm39)	I217N	possibly damaging	Het
Fcho2	A	T	13: 98,891,604 (GRCm39)	L386*	probably null	Het
Gm5114	C	A	7: 39,058,732 (GRCm39)	V296F	probably damaging	Het
Hc	A	G	2: 34,909,475 (GRCm39)		probably null	Het
Helz2	A	G	2: 180,879,581 (GRCm39)	V679A	probably benign	Het
Ifnar2	T	C	16: 91,201,060 (GRCm39)	F434L	probably benign	Het
Isl1	A	G	13: 116,439,857 (GRCm39)	S164P	possibly damaging	Het
Jup	C	T	11: 100,270,391 (GRCm39)	C372Y	probably damaging	Het
Kat2b	T	A	17: 53,967,116 (GRCm39)	Y621*	probably null	Het
Kif2b	T	C	11: 91,468,019 (GRCm39)	H88R	probably benign	Het
Kit	G	A	5: 75,799,722 (GRCm39)	V485I		Het
Klhl36	T	C	8: 120,596,887 (GRCm39)	L196P	probably damaging	Het
Klk1b27	T	A	7: 43,703,932 (GRCm39)	I25K	probably damaging	Het
Kmt2c	T	A	5: 25,519,387 (GRCm39)	Q2241L	possibly damaging	Het
Krt10	T	A	11: 99,278,434 (GRCm39)	N242Y	probably damaging	Het
Krt36	T	A	11: 99,993,129 (GRCm39)	I449F	probably damaging	Het
Med27	G	T	2: 29,236,875 (GRCm39)	V7F	possibly damaging	Het
Mettl25b	G	A	3: 87,837,282 (GRCm39)		probably benign	Het
Mxd1	C	T	6: 86,628,466 (GRCm39)	V145M	probably benign	Het
Mylip	T	A	13: 45,544,820 (GRCm39)	F81L	probably damaging	Het
Nceh1	T	A	3: 27,276,885 (GRCm39)	F69L	probably null	Het
Ndufs3	A	T	2: 90,732,773 (GRCm39)	N104K	probably damaging	Het
Nfxl1	A	C	5: 72,716,473 (GRCm39)	V46G	unknown	Het
Or51a39	T	C	7: 102,363,558 (GRCm39)	T21A	probably benign	Het
Or51f1e	T	G	7: 102,747,251 (GRCm39)	I101S	probably damaging	Het
Or5as1	T	A	2: 86,980,928 (GRCm39)	M26L	probably benign	Het
Or5m10b	A	G	2: 85,699,832 (GRCm39)	N299D	probably benign	Het
Palld	T	A	8: 62,137,883 (GRCm39)	H624L	probably damaging	Het
Pcbp2	T	G	15: 102,399,214 (GRCm39)	L343R	probably damaging	Het
Pclo	T	A	5: 14,572,208 (GRCm39)	I531N	unknown	Het
Phip	T	C	9: 82,809,017 (GRCm39)		probably benign	Het
Phldb2	A	T	16: 45,592,496 (GRCm39)		probably null	Het
Ppp2r5e	G	T	12: 75,500,492 (GRCm39)	T467N	possibly damaging	Het
Ptcd3	T	C	6: 71,880,431 (GRCm39)	I97M	probably benign	Het
Ptpn21	T	A	12: 98,655,284 (GRCm39)	Q561L	probably benign	Het
Rnf38	A	T	4: 44,149,079 (GRCm39)	H121Q	possibly damaging	Het
Sec1	A	G	7: 45,328,897 (GRCm39)	F50S	possibly damaging	Het
Sec62	A	G	3: 30,873,024 (GRCm39)	E369G	unknown	Het
Serpinb2	A	T	1: 107,452,390 (GRCm39)	I323F	probably damaging	Het
Sh3rf1	A	C	8: 61,837,860 (GRCm39)	T802P	probably benign	Het
Srd5a3	A	G	5: 76,301,493 (GRCm39)	R241G	probably benign	Het
Suox	T	C	10: 128,507,542 (GRCm39)	N162S	probably benign	Het
Tenm2	C	T	11: 35,942,688 (GRCm39)	C1327Y	probably damaging	Het
Terf2ip	A	G	8: 112,738,370 (GRCm39)	D86G	probably damaging	Het
Tmc1	G	A	19: 20,793,593 (GRCm39)	R523C	probably damaging	Het
Tmprss11e	T	A	5: 86,861,758 (GRCm39)	I263L	possibly damaging	Het
Tmtc4	T	C	14: 123,179,224 (GRCm39)		probably benign	Het
Tnfrsf22	T	A	7: 143,192,173 (GRCm39)	N171I	unknown	Het
Trpm3	A	G	19: 22,903,308 (GRCm39)	S1035G	probably damaging	Het
U2af1	C	T	17: 31,867,854 (GRCm39)	V72M	possibly damaging	Het
Uimc1	A	G	13: 55,233,447 (GRCm39)	C70R	possibly damaging	Het
Vmn2r83	A	G	10: 79,313,853 (GRCm39)	T154A	probably benign	Het
Xrn2	T	C	2: 146,871,304 (GRCm39)	I302T	probably damaging	Het
Zbtb6	A	G	2: 37,318,677 (GRCm39)	L417P	probably damaging	Het
Zdhhc14	C	T	17: 5,775,555 (GRCm39)	S269L	probably benign	Het
Zfp735	T	A	11: 73,602,699 (GRCm39)	F548I	possibly damaging	Het
Zfp809	T	C	9: 22,137,130 (GRCm39)		probably null	Het
Zranb3	A	T	1: 127,888,588 (GRCm39)	D832E	possibly damaging	Het

Other mutations in Ash1l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Ash1l	APN	3	88,889,019 (GRCm39)	missense	probably benign	0.19
IGL00819:Ash1l	APN	3	88,915,043 (GRCm39)	missense	possibly damaging	0.68
IGL00939:Ash1l	APN	3	88,942,543 (GRCm39)	missense	probably damaging	0.99
IGL01064:Ash1l	APN	3	88,979,791 (GRCm39)	missense	probably damaging	1.00
IGL01066:Ash1l	APN	3	88,891,942 (GRCm39)	missense	probably damaging	1.00
IGL01087:Ash1l	APN	3	88,971,209 (GRCm39)	missense	probably damaging	1.00
IGL01293:Ash1l	APN	3	88,890,836 (GRCm39)	missense	probably benign	0.01
IGL01541:Ash1l	APN	3	88,973,572 (GRCm39)	missense	probably damaging	1.00
IGL01863:Ash1l	APN	3	88,892,813 (GRCm39)	nonsense	probably null
IGL02326:Ash1l	APN	3	88,873,364 (GRCm39)	missense	probably benign	0.00
IGL02407:Ash1l	APN	3	88,979,855 (GRCm39)	missense	probably damaging	1.00
IGL02419:Ash1l	APN	3	88,892,872 (GRCm39)	missense	probably benign	0.00
IGL02422:Ash1l	APN	3	88,976,386 (GRCm39)	critical splice donor site	probably null
IGL02494:Ash1l	APN	3	88,973,525 (GRCm39)	nonsense	probably null
IGL02727:Ash1l	APN	3	88,930,344 (GRCm39)	missense	probably benign
IGL02732:Ash1l	APN	3	88,873,535 (GRCm39)	missense	probably damaging	1.00
IGL02817:Ash1l	APN	3	88,892,108 (GRCm39)	missense	probably damaging	1.00
IGL02887:Ash1l	APN	3	88,891,488 (GRCm39)	missense	probably benign	0.11
IGL03224:Ash1l	APN	3	88,942,575 (GRCm39)	splice site	probably benign
IGL03253:Ash1l	APN	3	88,891,981 (GRCm39)	missense	probably damaging	1.00
IGL03327:Ash1l	APN	3	88,930,390 (GRCm39)	missense	probably benign	0.02
IGL03398:Ash1l	APN	3	88,914,527 (GRCm39)	missense	probably benign	0.01
3-1:Ash1l	UTSW	3	88,873,633 (GRCm39)	missense	probably benign
BB008:Ash1l	UTSW	3	88,950,848 (GRCm39)	missense	probably damaging	1.00
BB018:Ash1l	UTSW	3	88,950,848 (GRCm39)	missense	probably damaging	1.00
R0068:Ash1l	UTSW	3	88,914,624 (GRCm39)	missense	probably benign	0.17
R0068:Ash1l	UTSW	3	88,914,624 (GRCm39)	missense	probably benign	0.17
R0239:Ash1l	UTSW	3	88,974,529 (GRCm39)	missense	possibly damaging	0.49
R0239:Ash1l	UTSW	3	88,974,529 (GRCm39)	missense	possibly damaging	0.49
R0395:Ash1l	UTSW	3	88,965,896 (GRCm39)	missense	probably damaging	1.00
R0477:Ash1l	UTSW	3	88,890,766 (GRCm39)	missense	probably benign	0.41
R0528:Ash1l	UTSW	3	88,889,584 (GRCm39)	missense	probably benign
R0543:Ash1l	UTSW	3	88,971,085 (GRCm39)	splice site	probably null
R0855:Ash1l	UTSW	3	88,961,761 (GRCm39)	missense	possibly damaging	0.82
R1147:Ash1l	UTSW	3	88,892,194 (GRCm39)	missense	possibly damaging	0.72
R1147:Ash1l	UTSW	3	88,892,194 (GRCm39)	missense	possibly damaging	0.72
R1163:Ash1l	UTSW	3	88,942,570 (GRCm39)	critical splice donor site	probably null
R1196:Ash1l	UTSW	3	88,890,623 (GRCm39)	missense	probably damaging	0.99
R1419:Ash1l	UTSW	3	88,892,204 (GRCm39)	missense	probably damaging	0.99
R1445:Ash1l	UTSW	3	88,914,659 (GRCm39)	missense	probably benign	0.02
R1466:Ash1l	UTSW	3	88,959,372 (GRCm39)	missense	probably damaging	1.00
R1466:Ash1l	UTSW	3	88,959,372 (GRCm39)	missense	probably damaging	1.00
R1480:Ash1l	UTSW	3	88,892,359 (GRCm39)	missense	probably damaging	1.00
R1506:Ash1l	UTSW	3	88,965,806 (GRCm39)	missense	probably damaging	0.99
R1537:Ash1l	UTSW	3	88,979,783 (GRCm39)	missense	probably damaging	0.99
R1584:Ash1l	UTSW	3	88,959,372 (GRCm39)	missense	probably damaging	1.00
R1669:Ash1l	UTSW	3	88,974,549 (GRCm39)	critical splice donor site	probably null
R1713:Ash1l	UTSW	3	88,983,531 (GRCm39)	missense	probably damaging	1.00
R1780:Ash1l	UTSW	3	88,873,291 (GRCm39)	missense	probably benign
R1793:Ash1l	UTSW	3	88,977,616 (GRCm39)	missense	probably damaging	1.00
R1881:Ash1l	UTSW	3	88,888,862 (GRCm39)	missense	probably benign	0.00
R1909:Ash1l	UTSW	3	88,891,835 (GRCm39)	missense	probably benign	0.29
R1938:Ash1l	UTSW	3	88,891,729 (GRCm39)	missense	probably damaging	0.98
R2035:Ash1l	UTSW	3	88,973,624 (GRCm39)	missense	probably benign	0.00
R2070:Ash1l	UTSW	3	88,873,510 (GRCm39)	missense	probably damaging	1.00
R2071:Ash1l	UTSW	3	88,873,510 (GRCm39)	missense	probably damaging	1.00
R2114:Ash1l	UTSW	3	88,890,571 (GRCm39)	missense	probably benign	0.00
R2116:Ash1l	UTSW	3	88,890,571 (GRCm39)	missense	probably benign	0.00
R2118:Ash1l	UTSW	3	88,892,602 (GRCm39)	missense	possibly damaging	0.80
R2143:Ash1l	UTSW	3	88,892,726 (GRCm39)	missense	probably benign	0.09
R2164:Ash1l	UTSW	3	88,892,726 (GRCm39)	missense	probably benign	0.09
R2210:Ash1l	UTSW	3	88,973,605 (GRCm39)	missense	probably damaging	1.00
R2247:Ash1l	UTSW	3	88,914,674 (GRCm39)	missense	possibly damaging	0.77
R2303:Ash1l	UTSW	3	88,933,733 (GRCm39)	missense	probably damaging	1.00
R2860:Ash1l	UTSW	3	88,961,785 (GRCm39)	missense	probably damaging	1.00
R2861:Ash1l	UTSW	3	88,961,785 (GRCm39)	missense	probably damaging	1.00
R3104:Ash1l	UTSW	3	88,961,693 (GRCm39)	missense	probably damaging	1.00
R4133:Ash1l	UTSW	3	88,889,567 (GRCm39)	missense	probably benign	0.00
R4164:Ash1l	UTSW	3	88,889,273 (GRCm39)	missense	probably damaging	0.97
R4270:Ash1l	UTSW	3	88,889,347 (GRCm39)	missense	probably benign	0.26
R4271:Ash1l	UTSW	3	88,889,347 (GRCm39)	missense	probably benign	0.26
R4287:Ash1l	UTSW	3	88,973,722 (GRCm39)	missense	probably damaging	0.99
R4409:Ash1l	UTSW	3	88,914,506 (GRCm39)	missense	probably damaging	0.99
R4459:Ash1l	UTSW	3	88,873,541 (GRCm39)	missense	probably damaging	0.99
R4487:Ash1l	UTSW	3	88,892,622 (GRCm39)	missense	possibly damaging	0.65
R4674:Ash1l	UTSW	3	88,979,783 (GRCm39)	missense	possibly damaging	0.80
R4739:Ash1l	UTSW	3	88,890,152 (GRCm39)	missense	probably benign	0.19
R4927:Ash1l	UTSW	3	88,892,641 (GRCm39)	missense	probably damaging	1.00
R5000:Ash1l	UTSW	3	88,965,941 (GRCm39)	missense	probably damaging	1.00
R5016:Ash1l	UTSW	3	88,889,630 (GRCm39)	missense	probably damaging	1.00
R5055:Ash1l	UTSW	3	88,930,519 (GRCm39)	critical splice donor site	probably null
R5081:Ash1l	UTSW	3	88,892,024 (GRCm39)	missense	probably damaging	1.00
R5082:Ash1l	UTSW	3	88,873,541 (GRCm39)	missense	probably damaging	0.99
R5090:Ash1l	UTSW	3	88,960,184 (GRCm39)	missense	probably damaging	1.00
R5113:Ash1l	UTSW	3	88,973,582 (GRCm39)	missense	probably damaging	0.99
R5408:Ash1l	UTSW	3	88,889,701 (GRCm39)	missense	probably damaging	1.00
R5452:Ash1l	UTSW	3	88,892,183 (GRCm39)	missense	possibly damaging	0.93
R5487:Ash1l	UTSW	3	88,888,733 (GRCm39)	missense	probably benign	0.17
R5610:Ash1l	UTSW	3	88,930,492 (GRCm39)	missense	probably damaging	1.00
R5624:Ash1l	UTSW	3	88,892,916 (GRCm39)	missense	probably damaging	1.00
R5682:Ash1l	UTSW	3	88,914,914 (GRCm39)	missense	probably damaging	0.99
R5712:Ash1l	UTSW	3	88,959,297 (GRCm39)	missense	probably damaging	0.99
R5719:Ash1l	UTSW	3	88,965,933 (GRCm39)	missense	probably damaging	1.00
R5719:Ash1l	UTSW	3	88,961,805 (GRCm39)	missense	possibly damaging	0.83
R5839:Ash1l	UTSW	3	88,890,658 (GRCm39)	missense	probably damaging	0.99
R5859:Ash1l	UTSW	3	88,976,300 (GRCm39)	missense	probably damaging	1.00
R5877:Ash1l	UTSW	3	88,888,891 (GRCm39)	missense	probably benign	0.00
R5940:Ash1l	UTSW	3	88,891,343 (GRCm39)	missense	probably damaging	0.96
R6026:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6027:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6029:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6033:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6033:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6034:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6034:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6035:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6035:Ash1l	UTSW	3	88,892,326 (GRCm39)	missense	probably damaging	1.00
R6089:Ash1l	UTSW	3	88,960,450 (GRCm39)	nonsense	probably null
R6110:Ash1l	UTSW	3	88,892,436 (GRCm39)	missense	probably damaging	1.00
R6168:Ash1l	UTSW	3	88,960,080 (GRCm39)	nonsense	probably null
R6200:Ash1l	UTSW	3	88,977,834 (GRCm39)	missense	probably damaging	1.00
R6290:Ash1l	UTSW	3	88,890,068 (GRCm39)	nonsense	probably null
R6331:Ash1l	UTSW	3	88,915,172 (GRCm39)	missense	probably benign	0.00
R6425:Ash1l	UTSW	3	88,891,087 (GRCm39)	missense	probably damaging	0.99
R6540:Ash1l	UTSW	3	88,892,368 (GRCm39)	missense	probably damaging	1.00
R6568:Ash1l	UTSW	3	88,959,344 (GRCm39)	missense	probably benign	0.09
R6828:Ash1l	UTSW	3	88,983,420 (GRCm39)	missense	probably benign	0.00
R6843:Ash1l	UTSW	3	88,892,695 (GRCm39)	missense	probably damaging	1.00
R6894:Ash1l	UTSW	3	88,890,298 (GRCm39)	missense	probably benign	0.00
R6976:Ash1l	UTSW	3	88,888,964 (GRCm39)	missense	possibly damaging	0.77
R7038:Ash1l	UTSW	3	88,889,978 (GRCm39)	missense	probably benign	0.00
R7073:Ash1l	UTSW	3	88,892,647 (GRCm39)	missense	probably damaging	1.00
R7133:Ash1l	UTSW	3	88,890,764 (GRCm39)	frame shift	probably null
R7150:Ash1l	UTSW	3	88,984,381 (GRCm39)	missense	probably damaging	1.00
R7205:Ash1l	UTSW	3	88,873,259 (GRCm39)	missense	probably benign	0.00
R7254:Ash1l	UTSW	3	88,977,816 (GRCm39)	missense	probably damaging	1.00
R7272:Ash1l	UTSW	3	88,961,941 (GRCm39)	splice site	probably null
R7288:Ash1l	UTSW	3	88,873,199 (GRCm39)	start gained	probably benign
R7319:Ash1l	UTSW	3	88,888,694 (GRCm39)	missense	probably benign	0.19
R7341:Ash1l	UTSW	3	88,889,066 (GRCm39)	missense	possibly damaging	0.93
R7342:Ash1l	UTSW	3	88,873,304 (GRCm39)	missense	possibly damaging	0.94
R7454:Ash1l	UTSW	3	88,891,172 (GRCm39)	missense	probably benign	0.16
R7677:Ash1l	UTSW	3	88,950,500 (GRCm39)	missense	probably damaging	1.00
R7822:Ash1l	UTSW	3	88,914,571 (GRCm39)	missense	probably benign
R7857:Ash1l	UTSW	3	88,891,616 (GRCm39)	nonsense	probably null
R7889:Ash1l	UTSW	3	88,873,345 (GRCm39)	missense	probably benign	0.00
R7898:Ash1l	UTSW	3	88,890,932 (GRCm39)	missense	possibly damaging	0.54
R7931:Ash1l	UTSW	3	88,950,848 (GRCm39)	missense	probably damaging	1.00
R7937:Ash1l	UTSW	3	88,977,624 (GRCm39)	nonsense	probably null
R7973:Ash1l	UTSW	3	88,960,164 (GRCm39)	missense	probably benign
R8119:Ash1l	UTSW	3	88,942,734 (GRCm39)	missense	probably damaging	1.00
R8157:Ash1l	UTSW	3	88,971,014 (GRCm39)	critical splice donor site	probably null
R8162:Ash1l	UTSW	3	88,977,553 (GRCm39)	missense	probably damaging	0.99
R8194:Ash1l	UTSW	3	88,960,062 (GRCm39)	missense	probably damaging	1.00
R8306:Ash1l	UTSW	3	88,873,259 (GRCm39)	missense	probably benign	0.00
R8497:Ash1l	UTSW	3	88,914,951 (GRCm39)	missense	probably benign	0.02
R8558:Ash1l	UTSW	3	88,891,713 (GRCm39)	missense	probably damaging	0.96
R8744:Ash1l	UTSW	3	88,965,890 (GRCm39)	missense	possibly damaging	0.89
R8923:Ash1l	UTSW	3	88,892,974 (GRCm39)	missense	possibly damaging	0.51
R8970:Ash1l	UTSW	3	88,976,307 (GRCm39)	missense	probably benign	0.00
R9002:Ash1l	UTSW	3	88,888,715 (GRCm39)	missense	probably benign	0.17
R9023:Ash1l	UTSW	3	88,892,576 (GRCm39)	missense	probably damaging	1.00
R9032:Ash1l	UTSW	3	88,891,529 (GRCm39)	missense	probably benign	0.00
R9032:Ash1l	UTSW	3	88,889,294 (GRCm39)	missense	probably benign	0.19
R9049:Ash1l	UTSW	3	88,914,671 (GRCm39)	missense	probably benign
R9085:Ash1l	UTSW	3	88,891,529 (GRCm39)	missense	probably benign	0.00
R9085:Ash1l	UTSW	3	88,889,294 (GRCm39)	missense	probably benign	0.19
R9130:Ash1l	UTSW	3	88,965,848 (GRCm39)	nonsense	probably null
R9149:Ash1l	UTSW	3	88,914,530 (GRCm39)	missense	probably benign
R9294:Ash1l	UTSW	3	88,890,297 (GRCm39)	missense	possibly damaging	0.90
R9365:Ash1l	UTSW	3	88,889,207 (GRCm39)	missense	possibly damaging	0.63
R9450:Ash1l	UTSW	3	88,915,139 (GRCm39)	missense	possibly damaging	0.86
R9542:Ash1l	UTSW	3	88,950,566 (GRCm39)	missense	probably damaging	1.00
R9558:Ash1l	UTSW	3	88,889,521 (GRCm39)	missense	probably benign	0.02
R9572:Ash1l	UTSW	3	88,960,188 (GRCm39)	missense	probably damaging	1.00
R9688:Ash1l	UTSW	3	88,892,024 (GRCm39)	missense	probably damaging	1.00
R9736:Ash1l	UTSW	3	88,891,733 (GRCm39)	missense	probably damaging	1.00
R9765:Ash1l	UTSW	3	88,930,500 (GRCm39)	missense	probably damaging	1.00
R9789:Ash1l	UTSW	3	88,873,373 (GRCm39)	missense	probably benign
X0017:Ash1l	UTSW	3	88,891,892 (GRCm39)	missense	probably benign	0.45
X0019:Ash1l	UTSW	3	88,977,863 (GRCm39)	missense	probably damaging	1.00
X0021:Ash1l	UTSW	3	88,890,511 (GRCm39)	missense	probably benign	0.10
Z1088:Ash1l	UTSW	3	88,890,016 (GRCm39)	missense	probably benign	0.00
Z1176:Ash1l	UTSW	3	88,950,524 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGAAGAGGATCCTCGCAAGC -3'
(R):5'- TAGCATCCCTTATAGCCCATTTAG -3'

Sequencing Primer
(F):5'- GGATGCACAGCAACAGTTTTC -3'
(R):5'- TCCCTTATAGCCCATTTAGAAACAAC -3'

Posted On

2021-10-11