Mutagenetix > Incidental Mutation

Incidental Mutation 'R8974:Sidt1'

683339

Institutional Source

Beutler Lab

Gene Symbol

Sidt1

Ensembl Gene

ENSMUSG00000022696

Gene Name

SID1 transmembrane family, member 1

Synonyms

B830021E24Rik

MMRRC Submission

068808-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8974 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

44060543-44153559 bp(-) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

A to T at 44101980 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Arginine at position 62 (*62R)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047446] [ENSMUST00000136381]

AlphaFold

Q6AXF6

Predicted Effect

probably benign
Transcript: ENSMUST00000047446

SMART Domains

Protein: ENSMUSP00000038433
Gene: ENSMUSG00000022696

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
low complexity region	36	50	N/A	INTRINSIC
Pfam:SID-1_RNA_chan	184	832	1.8e-290	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000136381

SMART Domains

Protein: ENSMUSP00000115372
Gene: ENSMUSG00000022696

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
low complexity region	36	50	N/A	INTRINSIC
Pfam:SID-1_RNA_chan	184	827	1.3e-251	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000147032
AA Change: *62R

SMART Domains

Protein: ENSMUSP00000114424
Gene: ENSMUSG00000022696
AA Change: *62R

Domain	Start	End	E-Value	Type
transmembrane domain	24	46	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acat3	A	G	17: 13,143,716 (GRCm39)	V342A	probably damaging	Het
Acot4	T	C	12: 84,090,515 (GRCm39)	F404S	probably benign	Het
Alpk1	T	C	3: 127,473,580 (GRCm39)	T808A	probably benign	Het
Apba3	T	C	10: 81,109,032 (GRCm39)	V154A		Het
Arhgap27	A	T	11: 103,224,756 (GRCm39)	F476I	possibly damaging	Het
Asah2	A	T	19: 32,030,305 (GRCm39)	D122E	probably benign	Het
Asb10	T	C	5: 24,745,209 (GRCm39)	I47V	probably benign	Het
Catsperd	A	G	17: 56,959,525 (GRCm39)	T322A	possibly damaging	Het
Ccdc142	T	C	6: 83,078,963 (GRCm39)	V100A	probably benign	Het
Cdc5l	A	T	17: 45,715,621 (GRCm39)	C664S	possibly damaging	Het
Cep250	C	T	2: 155,812,042 (GRCm39)	A446V	unknown	Het
Cep89	A	G	7: 35,097,493 (GRCm39)	S40G	probably damaging	Het
Csmd2	A	G	4: 128,446,380 (GRCm39)	K3315R		Het
Cyp2d34	T	C	15: 82,500,537 (GRCm39)	E434G	probably damaging	Het
Degs1	A	G	1: 182,107,278 (GRCm39)	Y30H	probably benign	Het
Eps8l1	A	G	7: 4,474,817 (GRCm39)	D266G	probably damaging	Het
Fam168a	A	T	7: 100,484,611 (GRCm39)	T234S	probably benign	Het
Fzd3	G	T	14: 65,472,440 (GRCm39)	Q443K	possibly damaging	Het
Galnt7	A	G	8: 58,105,934 (GRCm39)	S27P		Het
Gpr162	T	C	6: 124,837,876 (GRCm39)	D258G	probably damaging	Het
Hdac2	A	G	10: 36,862,340 (GRCm39)	Y68C	probably damaging	Het
Lmbr1l	A	G	15: 98,810,408 (GRCm39)	C68R	probably damaging	Het
Loxhd1	A	G	18: 77,518,899 (GRCm39)	T1902A	possibly damaging	Het
Lrrc4c	T	C	2: 97,459,992 (GRCm39)	M206T	probably damaging	Het
Lrrc75b	T	C	10: 75,389,848 (GRCm39)	T168A	probably benign	Het
Magi1	A	G	6: 93,674,223 (GRCm39)	F1003L	probably damaging	Het
Map1s	T	C	8: 71,366,994 (GRCm39)	L633P	probably damaging	Het
Mapk11	G	A	15: 89,028,014 (GRCm39)	T341I	probably benign	Het
Mycbp2	A	T	14: 103,361,857 (GRCm39)	V4441D	probably damaging	Het
Nin	T	C	12: 70,124,932 (GRCm39)	E130G		Het
Nod2	T	A	8: 89,390,433 (GRCm39)	Y247N	probably damaging	Het
Nudcd3	A	T	11: 6,143,163 (GRCm39)	V169E	probably benign	Het
Or2w2	A	T	13: 21,758,203 (GRCm39)	I141N	probably damaging	Het
Or51v15-ps1	T	C	7: 103,278,489 (GRCm39)	Y226C	unknown	Het
Or6c200-ps1	A	C	10: 128,869,905 (GRCm39)	C269G	probably damaging	Het
Pcdh9	A	C	14: 94,125,113 (GRCm39)	N352K	probably benign	Het
Pfpl	G	A	19: 12,405,839 (GRCm39)	G30D	probably damaging	Het
Pi15	A	G	1: 17,691,675 (GRCm39)	D159G	possibly damaging	Het
Plxna4	G	T	6: 32,216,447 (GRCm39)	R543S	possibly damaging	Het
Ppp1r12c	C	T	7: 4,486,698 (GRCm39)	E543K	probably damaging	Het
Prkdc	T	A	16: 15,617,726 (GRCm39)		probably null	Het
Proca1	T	C	11: 78,096,144 (GRCm39)	S172P	probably damaging	Het
Ralgapa1	C	A	12: 55,723,791 (GRCm39)	M1578I	probably benign	Het
Rnf103	C	A	6: 71,486,092 (GRCm39)	A241E	probably damaging	Het
Rorb	T	C	19: 18,955,070 (GRCm39)	D182G	probably benign	Het
Ryr3	T	C	2: 112,742,624 (GRCm39)	K485E	possibly damaging	Het
Sec24d	G	A	3: 123,099,498 (GRCm39)	D324N	probably damaging	Het
Serpina3f	T	A	12: 104,183,642 (GRCm39)	I168N	probably damaging	Het
Slc22a27	A	C	19: 7,903,751 (GRCm39)	S129A	probably damaging	Het
Spata31e1	T	C	13: 49,939,556 (GRCm39)	D718G	probably benign	Het
Stab1	A	G	14: 30,882,779 (GRCm39)	V408A	probably benign	Het
Svil	G	T	18: 5,099,650 (GRCm39)	G1729W	probably damaging	Het
Tbx20	A	G	9: 24,681,082 (GRCm39)	S137P	probably damaging	Het
Tcstv2c	T	A	13: 120,616,486 (GRCm39)	D108E	probably benign	Het
Trem2	G	A	17: 48,655,599 (GRCm39)	R52Q	probably damaging	Het
Trim55	T	C	3: 19,699,603 (GRCm39)	S112P	probably damaging	Het
Vmn1r172	A	T	7: 23,359,840 (GRCm39)	M242L	probably benign	Het
Vmn2r91	G	A	17: 18,325,636 (GRCm39)	A85T	probably benign	Het
Zc3h3	T	C	15: 75,657,452 (GRCm39)	T580A	probably benign	Het
Zfp451	A	G	1: 33,816,535 (GRCm39)	C472R	probably damaging	Het

Other mutations in Sidt1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00709:Sidt1	APN	16	44,082,374 (GRCm39)	splice site	probably benign
IGL01103:Sidt1	APN	16	44,063,906 (GRCm39)	nonsense	probably null
IGL01725:Sidt1	APN	16	44,104,645 (GRCm39)	missense	probably benign	0.06
IGL02000:Sidt1	APN	16	44,106,732 (GRCm39)	missense	probably damaging	0.98
IGL02266:Sidt1	APN	16	44,075,348 (GRCm39)	missense	possibly damaging	0.90
IGL02309:Sidt1	APN	16	44,075,343 (GRCm39)	missense	probably benign	0.00
IGL02898:Sidt1	APN	16	44,102,858 (GRCm39)	missense	possibly damaging	0.87
R0282:Sidt1	UTSW	16	44,102,249 (GRCm39)	missense	possibly damaging	0.79
R0525:Sidt1	UTSW	16	44,079,809 (GRCm39)	missense	possibly damaging	0.64
R0927:Sidt1	UTSW	16	44,063,895 (GRCm39)	missense	probably benign	0.00
R1806:Sidt1	UTSW	16	44,102,234 (GRCm39)	missense	possibly damaging	0.46
R1911:Sidt1	UTSW	16	44,102,234 (GRCm39)	missense	possibly damaging	0.81
R3843:Sidt1	UTSW	16	44,104,587 (GRCm39)	missense	probably benign	0.04
R3848:Sidt1	UTSW	16	44,076,322 (GRCm39)	intron	probably benign
R4023:Sidt1	UTSW	16	44,102,249 (GRCm39)	missense	possibly damaging	0.79
R4026:Sidt1	UTSW	16	44,102,249 (GRCm39)	missense	possibly damaging	0.79
R4495:Sidt1	UTSW	16	44,102,841 (GRCm39)	missense	probably damaging	1.00
R4603:Sidt1	UTSW	16	44,075,389 (GRCm39)	missense	probably damaging	0.98
R4707:Sidt1	UTSW	16	44,090,221 (GRCm39)	nonsense	probably null
R5322:Sidt1	UTSW	16	44,101,985 (GRCm39)	intron	probably benign
R5921:Sidt1	UTSW	16	44,094,098 (GRCm39)	splice site	probably benign
R5980:Sidt1	UTSW	16	44,083,675 (GRCm39)	nonsense	probably null
R5982:Sidt1	UTSW	16	44,082,071 (GRCm39)	missense	probably damaging	1.00
R6063:Sidt1	UTSW	16	44,079,829 (GRCm39)	missense	probably benign	0.01
R6337:Sidt1	UTSW	16	44,121,298 (GRCm39)	splice site	probably null
R6392:Sidt1	UTSW	16	44,111,657 (GRCm39)	missense	possibly damaging	0.47
R6855:Sidt1	UTSW	16	44,065,706 (GRCm39)	missense	probably null	1.00
R7092:Sidt1	UTSW	16	44,120,192 (GRCm39)	missense	possibly damaging	0.89
R7099:Sidt1	UTSW	16	44,063,860 (GRCm39)	missense	probably damaging	1.00
R7448:Sidt1	UTSW	16	44,106,763 (GRCm39)	nonsense	probably null
R7574:Sidt1	UTSW	16	44,079,848 (GRCm39)	missense	probably damaging	1.00
R8265:Sidt1	UTSW	16	44,088,250 (GRCm39)	missense	possibly damaging	0.79
R8379:Sidt1	UTSW	16	44,106,755 (GRCm39)	missense	probably benign	0.14
R8460:Sidt1	UTSW	16	44,107,705 (GRCm39)	nonsense	probably null
R8480:Sidt1	UTSW	16	44,065,529 (GRCm39)	missense	probably damaging	1.00
R8762:Sidt1	UTSW	16	44,152,707 (GRCm39)	missense	probably benign	0.16
R8954:Sidt1	UTSW	16	44,082,390 (GRCm39)	missense	probably benign	0.04
R9362:Sidt1	UTSW	16	44,078,316 (GRCm39)	missense	possibly damaging	0.96
R9451:Sidt1	UTSW	16	44,075,392 (GRCm39)	critical splice acceptor site	probably null
R9669:Sidt1	UTSW	16	44,102,243 (GRCm39)	missense	probably damaging	1.00
R9737:Sidt1	UTSW	16	44,102,243 (GRCm39)	missense	probably damaging	1.00
Z1176:Sidt1	UTSW	16	44,079,845 (GRCm39)	missense	possibly damaging	0.90
Z1191:Sidt1	UTSW	16	44,078,294 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CAGCTACGGACTATACCTCAAG -3'
(R):5'- AGGTAGGTGCACAGGTTCTTC -3'

Sequencing Primer
(F):5'- CGATTAACTTTTCGACAGAATGCTTC -3'
(R):5'- GGTGCACAGGTTCTTCCTCCC -3'

Posted On

2021-10-11