Incidental Mutation 'R8975:Stk33'
ID 683385
Institutional Source Beutler Lab
Gene Symbol Stk33
Ensembl Gene ENSMUSG00000031027
Gene Name serine/threonine kinase 33
Synonyms 4921505G21Rik
MMRRC Submission 068809-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.068) question?
Stock # R8975 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 108878430-109038288 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 108935280 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 171 (I171F)
Ref Sequence ENSEMBL: ENSMUSP00000102356 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000090414] [ENSMUST00000106745] [ENSMUST00000121378] [ENSMUST00000121748] [ENSMUST00000141210]
AlphaFold Q924X7
Predicted Effect probably damaging
Transcript: ENSMUST00000090414
AA Change: I171F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000087897
Gene: ENSMUSG00000031027
AA Change: I171F

DomainStartEndE-ValueType
S_TKc 111 377 4.7e-102 SMART
low complexity region 399 414 N/A INTRINSIC
low complexity region 445 467 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000106745
AA Change: I171F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000102356
Gene: ENSMUSG00000031027
AA Change: I171F

DomainStartEndE-ValueType
S_TKc 111 377 4.7e-102 SMART
low complexity region 399 414 N/A INTRINSIC
low complexity region 445 467 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000121378
AA Change: I48F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000112560
Gene: ENSMUSG00000031027
AA Change: I48F

DomainStartEndE-ValueType
S_TKc 2 254 1.01e-83 SMART
low complexity region 276 291 N/A INTRINSIC
low complexity region 322 344 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000121748
AA Change: I171F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000112515
Gene: ENSMUSG00000031027
AA Change: I171F

DomainStartEndE-ValueType
S_TKc 111 377 4.8e-102 SMART
low complexity region 399 414 N/A INTRINSIC
low complexity region 445 467 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000141210
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (64/64)
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700018F24Rik A G 5: 144,980,192 (GRCm39) D47G probably damaging Het
4930550C14Rik A T 9: 53,334,258 (GRCm39) K167M possibly damaging Het
Abhd16b A G 2: 181,135,806 (GRCm39) N236S probably benign Het
Ahnak T G 19: 8,990,101 (GRCm39) V3795G probably damaging Het
Atg2b A T 12: 105,602,725 (GRCm39) M1606K probably damaging Het
Bmt2 A T 6: 13,630,611 (GRCm39) I187K probably damaging Het
Cep250 C T 2: 155,812,042 (GRCm39) A446V unknown Het
Cep290 A G 10: 100,349,782 (GRCm39) N610D possibly damaging Het
Ctps1 C T 4: 120,406,743 (GRCm39) D338N probably benign Het
Ctsz A T 2: 174,275,421 (GRCm39) I118N probably benign Het
Depdc1b A G 13: 108,525,094 (GRCm39) E483G probably null Het
Dhh T C 15: 98,795,976 (GRCm39) S60G probably damaging Het
Dpagt1 T C 9: 44,237,446 (GRCm39) probably benign Het
Dpy19l1 T C 9: 24,359,102 (GRCm39) K162R probably damaging Het
Dynap T C 18: 70,375,093 (GRCm39) T41A possibly damaging Het
Dyrk4 T C 6: 126,871,783 (GRCm39) N162D probably benign Het
Efcab3 A G 11: 104,954,415 (GRCm39) K5090E probably benign Het
Eml5 T A 12: 98,776,829 (GRCm39) T1429S probably damaging Het
Fbn2 C T 18: 58,286,928 (GRCm39) G244R probably damaging Het
Fer1l5 A G 1: 36,456,897 (GRCm39) D1625G probably benign Het
Frmd4b G A 6: 97,283,477 (GRCm39) T484M possibly damaging Het
Hhat T C 1: 192,431,250 (GRCm39) E33G probably damaging Het
Ifngr1 A G 10: 19,485,360 (GRCm39) D453G probably damaging Het
Itpr3 T A 17: 27,335,628 (GRCm39) I2246N possibly damaging Het
Larp4b T G 13: 9,195,537 (GRCm39) I212S probably damaging Het
Lilra5 T C 7: 4,241,636 (GRCm39) V145A probably benign Het
Map1lc3a A G 2: 155,118,926 (GRCm39) K30R possibly damaging Het
Mapk8ip1 A G 2: 92,215,166 (GRCm39) Y652H probably damaging Het
Med23 A G 10: 24,780,334 (GRCm39) E994G probably benign Het
Mep1b A T 18: 21,208,714 (GRCm39) D30V probably benign Het
Mki67 C A 7: 135,297,364 (GRCm39) A2557S possibly damaging Het
Mki67 T C 7: 135,300,129 (GRCm39) D1635G probably benign Het
Msantd5f6 A G 4: 73,320,167 (GRCm39) L204P probably damaging Het
Mup3 A T 4: 62,003,472 (GRCm39) D132E probably benign Het
Myo15b A T 11: 115,781,780 (GRCm39) T2830S unknown Het
Myocd T A 11: 65,069,287 (GRCm39) H979L probably damaging Het
Nvl A T 1: 180,958,001 (GRCm39) N260K probably benign Het
Or2a57 T A 6: 43,213,056 (GRCm39) H171Q probably benign Het
Or2d2b T A 7: 106,705,664 (GRCm39) T135S probably benign Het
Or4d2 T A 11: 87,784,645 (GRCm39) Y35F probably damaging Het
Pdha2 C A 3: 140,917,649 (GRCm39) probably benign Het
Peg10 GC GCTCC 6: 4,756,452 (GRCm39) probably benign Het
Phtf2 A C 5: 20,969,249 (GRCm39) V687G probably damaging Het
Plagl2 C T 2: 153,074,346 (GRCm39) G185D possibly damaging Het
Ppp1r3e A G 14: 55,113,946 (GRCm39) V242A probably benign Het
Proser3 A G 7: 30,239,458 (GRCm39) S549P possibly damaging Het
Prrc2c A G 1: 162,533,630 (GRCm39) V995A unknown Het
Ptpn13 A G 5: 103,649,266 (GRCm39) H223R probably benign Het
Rsf1 CG CGTCGGCGGGG 7: 97,229,115 (GRCm39) probably benign Het
Scn11a T C 9: 119,587,565 (GRCm39) E1393G probably damaging Het
Sipa1l1 T A 12: 82,479,612 (GRCm39) S1432T possibly damaging Het
Slc10a5 G C 3: 10,399,670 (GRCm39) A330G probably benign Het
Slc2a5 A C 4: 150,224,270 (GRCm39) N293H probably damaging Het
Spmap1 A T 11: 97,663,572 (GRCm39) Y93N probably damaging Het
Spmip4 C T 6: 50,561,391 (GRCm39) R176H probably damaging Het
Sptbn1 T C 11: 30,073,869 (GRCm39) E1400G possibly damaging Het
Syne1 G A 10: 5,161,945 (GRCm39) H5450Y probably benign Het
Tbcd G C 11: 121,387,679 (GRCm39) probably benign Het
Tkt T A 14: 30,288,884 (GRCm39) probably benign Het
Tpra1 A T 6: 88,888,726 (GRCm39) M303L probably benign Het
Ubr3 T C 2: 69,752,651 (GRCm39) S303P probably damaging Het
Uqcrc1 T C 9: 108,776,721 (GRCm39) F341S probably damaging Het
Vmn1r223 T C 13: 23,434,248 (GRCm39) S281P possibly damaging Het
Vmn1r32 C A 6: 66,530,189 (GRCm39) A196S probably benign Het
Zfp644 G T 5: 106,785,467 (GRCm39) T360N probably benign Het
Other mutations in Stk33
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01137:Stk33 APN 7 108,928,775 (GRCm39) missense probably benign 0.02
IGL01467:Stk33 APN 7 108,928,796 (GRCm39) missense probably damaging 0.99
IGL01558:Stk33 APN 7 108,940,491 (GRCm39) intron probably benign
IGL01775:Stk33 APN 7 108,911,574 (GRCm39) missense possibly damaging 0.92
R0052:Stk33 UTSW 7 108,878,876 (GRCm39) missense possibly damaging 0.46
R0336:Stk33 UTSW 7 108,930,681 (GRCm39) missense probably benign 0.01
R0394:Stk33 UTSW 7 108,940,696 (GRCm39) missense probably benign
R0579:Stk33 UTSW 7 108,924,904 (GRCm39) missense probably damaging 0.99
R0727:Stk33 UTSW 7 108,920,725 (GRCm39) missense probably damaging 0.96
R1363:Stk33 UTSW 7 108,879,028 (GRCm39) missense probably benign 0.06
R1574:Stk33 UTSW 7 108,879,027 (GRCm39) missense probably benign 0.01
R1574:Stk33 UTSW 7 108,879,027 (GRCm39) missense probably benign 0.01
R2153:Stk33 UTSW 7 108,940,527 (GRCm39) missense probably benign 0.01
R4366:Stk33 UTSW 7 108,879,002 (GRCm39) missense probably benign 0.06
R4896:Stk33 UTSW 7 108,926,802 (GRCm39) missense probably damaging 1.00
R4994:Stk33 UTSW 7 108,939,605 (GRCm39) missense probably benign 0.08
R5283:Stk33 UTSW 7 108,935,334 (GRCm39) missense possibly damaging 0.69
R6339:Stk33 UTSW 7 108,920,672 (GRCm39) missense probably benign 0.03
R6547:Stk33 UTSW 7 108,920,042 (GRCm39) missense possibly damaging 0.73
R6717:Stk33 UTSW 7 108,926,823 (GRCm39) missense possibly damaging 0.51
R6894:Stk33 UTSW 7 108,935,269 (GRCm39) missense possibly damaging 0.70
R9168:Stk33 UTSW 7 108,928,747 (GRCm39) missense probably damaging 0.99
R9175:Stk33 UTSW 7 108,920,724 (GRCm39) missense probably damaging 1.00
R9204:Stk33 UTSW 7 108,940,686 (GRCm39) missense probably benign 0.00
R9416:Stk33 UTSW 7 108,940,689 (GRCm39) missense probably benign 0.39
Z1176:Stk33 UTSW 7 108,935,266 (GRCm39) missense possibly damaging 0.51
Predicted Primers PCR Primer
(F):5'- CTAATGTGCCACCCAGAACATG -3'
(R):5'- CACTATTCAAAACTGTAGGAAAGGG -3'

Sequencing Primer
(F):5'- GAAGTCCTTCAGAATGTTCTCATC -3'
(R):5'- TAGGCTGGAAGTTCTGCA -3'
Posted On 2021-10-11