Mutagenetix > Incidental Mutation

Incidental Mutation 'R8978:Trim25'

683583

Institutional Source

Beutler Lab

Gene Symbol

Trim25

Ensembl Gene

ENSMUSG00000000275

Gene Name

tripartite motif-containing 25

Synonyms

Zfp147, estrogen-responsive finger protein

MMRRC Submission

068811-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8978 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

88890202-88911119 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88907027 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 462 (V462A)

Ref Sequence

ENSEMBL: ENSMUSP00000103528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000284] [ENSMUST00000100627] [ENSMUST00000107896]

AlphaFold

Q61510

PDB Structure

PRY-SPRY domain of Trim25 [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000000284
AA Change: V454A

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000000284
Gene: ENSMUSG00000000275
AA Change: V454A

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:SPEC	189	288	5e-34	BLAST
PDB:4LTB\|B	189	380	7e-69	PDB
PRY	453	505	3.44e-17	SMART
SPRY	506	626	9.62e-31	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000100627

SMART Domains

Protein: ENSMUSP00000098192
Gene: ENSMUSG00000000275

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:BBOX	151	186	3e-8	BLAST
Blast:SPEC	189	288	2e-37	BLAST
PDB:4LTB\|B	189	380	2e-71	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000107896
AA Change: V462A

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000103528
Gene: ENSMUSG00000000275
AA Change: V462A

Domain	Start	End	E-Value	Type
RING	13	53	8.04e-9	SMART
Blast:SPEC	189	288	3e-34	BLAST
PDB:4LTB\|B	189	380	8e-69	PDB
low complexity region	382	393	N/A	INTRINSIC
PRY	461	513	3.44e-17	SMART
SPRY	514	634	9.62e-31	SMART

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.8%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Engineered mutations result in a compromised response to estrogen resulting in functional but small uteri. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg4	A	G	9: 44,192,395 (GRCm39)	F224L	probably benign	Het
Adam39	A	G	8: 41,278,707 (GRCm39)	D366G	probably damaging	Het
Adamts14	T	A	10: 61,038,795 (GRCm39)	E902V	probably damaging	Het
Adamts6	T	C	13: 104,512,247 (GRCm39)	C490R	probably damaging	Het
Btbd3	T	C	2: 138,126,055 (GRCm39)	V413A	possibly damaging	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,128,173 (GRCm39)		probably benign	Het
Cdadc1	G	T	14: 59,813,197 (GRCm39)	N403K	probably damaging	Het
Cdc27	C	T	11: 104,399,211 (GRCm39)	V734I	possibly damaging	Het
Celf3	A	G	3: 94,392,667 (GRCm39)	D122G	probably benign	Het
Chgb	T	C	2: 132,634,498 (GRCm39)	S147P	probably benign	Het
Col9a1	G	A	1: 24,278,396 (GRCm39)	G808D	probably damaging	Het
Cpne7	A	G	8: 123,861,177 (GRCm39)		probably null	Het
Crhr1	T	C	11: 104,064,480 (GRCm39)	F330S	possibly damaging	Het
Csmd1	A	C	8: 15,971,056 (GRCm39)	N3086K	probably benign	Het
Ctnnb1	A	G	9: 120,786,650 (GRCm39)	D624G	probably damaging	Het
Dcaf8	G	A	1: 172,022,124 (GRCm39)	R554H	probably benign	Het
Dmbt1	T	C	7: 130,639,611 (GRCm39)	Y50H	possibly damaging	Het
Dmxl1	T	C	18: 50,055,679 (GRCm39)	L2573P	probably benign	Het
Ebf2	G	A	14: 67,661,548 (GRCm39)	G559S	probably benign	Het
Fancd2	A	G	6: 113,562,507 (GRCm39)		probably benign	Het
Fkbp10	A	T	11: 100,313,936 (GRCm39)	I427F	probably benign	Het
Gipc1	T	C	8: 84,389,046 (GRCm39)		probably null	Het
Gm1110	A	T	9: 26,807,095 (GRCm39)		probably benign	Het
Gsdmc3	A	G	15: 63,731,455 (GRCm39)	L359P	probably damaging	Het
Hspg2	A	G	4: 137,291,341 (GRCm39)	D3897G	probably benign	Het
Ift122	T	A	6: 115,902,769 (GRCm39)	M1117K	possibly damaging	Het
Itgbl1	A	T	14: 124,209,617 (GRCm39)	D456V	probably damaging	Het
Itprid2	T	A	2: 79,491,257 (GRCm39)	L1014Q	probably damaging	Het
Kctd1	G	T	18: 15,119,491 (GRCm39)	L675I		Het
Kyat3	C	T	3: 142,443,596 (GRCm39)	T403M	probably benign	Het
Ltbp2	T	C	12: 84,834,164 (GRCm39)	T1442A	probably benign	Het
Mier2	A	C	10: 79,376,790 (GRCm39)	S45R	unknown	Het
Mon2	T	A	10: 122,871,469 (GRCm39)	K383*	probably null	Het
Mroh4	G	T	15: 74,499,473 (GRCm39)	T136K	probably benign	Het
Mtif3	A	T	5: 146,895,846 (GRCm39)	S80R	probably benign	Het
Myh2	A	C	11: 67,068,188 (GRCm39)	E272A	probably damaging	Het
Myh2	A	G	11: 67,080,323 (GRCm39)	Q1179R	probably damaging	Het
Nfatc3	G	A	8: 106,835,402 (GRCm39)	C916Y	probably benign	Het
Or13a17	A	T	7: 140,271,642 (GRCm39)	T275S	probably benign	Het
Or2w2	T	A	13: 21,758,279 (GRCm39)	T116S	probably benign	Het
Or7g21	T	A	9: 19,032,582 (GRCm39)	C107*	probably null	Het
Orc2	T	C	1: 58,511,499 (GRCm39)	D370G	possibly damaging	Het
Pate7	A	T	9: 35,688,069 (GRCm39)		probably benign	Het
Pcdhga6	T	A	18: 37,840,716 (GRCm39)	N145K	probably benign	Het
Pih1d2	A	G	9: 50,536,232 (GRCm39)	M296V	probably benign	Het
Prickle4	A	T	17: 47,999,772 (GRCm39)		probably benign	Het
Rabl6	G	A	2: 25,477,541 (GRCm39)	P303L	probably damaging	Het
Rara	T	G	11: 98,862,595 (GRCm39)	I332S	probably damaging	Het
Reln	A	G	5: 22,090,512 (GRCm39)	F3449L	possibly damaging	Het
Rhoq	T	C	17: 87,271,767 (GRCm39)	L61P		Het
Rigi	A	G	4: 40,239,650 (GRCm39)	I16T	probably damaging	Het
Slc6a15	G	A	10: 103,230,953 (GRCm39)	W226*	probably null	Het
Spef2	A	T	15: 9,725,263 (GRCm39)	S165T	possibly damaging	Het
Stab2	T	C	10: 86,785,782 (GRCm39)	N620S	possibly damaging	Het
Suds3	A	G	5: 117,232,973 (GRCm39)		probably null	Het
Sult1b1	A	G	5: 87,682,900 (GRCm39)	I15T	possibly damaging	Het
Synm	C	A	7: 67,384,672 (GRCm39)	V997L	probably damaging	Het
Tgfbi	C	A	13: 56,778,391 (GRCm39)	D387E	probably benign	Het
Thrb	T	C	14: 17,981,886 (GRCm38)	C4R	possibly damaging	Het
Tk2	A	G	8: 104,957,809 (GRCm39)	V179A	possibly damaging	Het
Tub	T	C	7: 108,629,393 (GRCm39)	Y483H	probably damaging	Het
Vav1	A	G	17: 57,603,710 (GRCm39)	T131A	probably benign	Het
Vav1	A	G	17: 57,631,650 (GRCm39)	T781A	probably benign	Het
Zbtb34	A	G	2: 33,301,048 (GRCm39)	S498P	possibly damaging	Het
Zfp568	T	A	7: 29,716,683 (GRCm39)	H194Q	probably benign	Het
Zfp683	A	T	4: 133,781,239 (GRCm39)	Q18L	probably benign	Het

Other mutations in Trim25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01707:Trim25	APN	11	88,890,517 (GRCm39)	missense	probably damaging	0.96
IGL02398:Trim25	APN	11	88,890,630 (GRCm39)	missense	probably damaging	1.00
IGL03150:Trim25	APN	11	88,890,831 (GRCm39)	missense	probably damaging	1.00
R0003:Trim25	UTSW	11	88,906,598 (GRCm39)	missense	probably benign	0.01
R0184:Trim25	UTSW	11	88,890,466 (GRCm39)	missense	probably damaging	1.00
R0707:Trim25	UTSW	11	88,890,564 (GRCm39)	missense	probably benign	0.03
R1855:Trim25	UTSW	11	88,906,407 (GRCm39)	missense	probably benign	0.04
R1936:Trim25	UTSW	11	88,895,576 (GRCm39)	missense	probably benign	0.03
R2229:Trim25	UTSW	11	88,907,447 (GRCm39)	missense	probably damaging	0.97
R3401:Trim25	UTSW	11	88,901,707 (GRCm39)	missense	probably benign
R5159:Trim25	UTSW	11	88,890,358 (GRCm39)	missense	probably benign	0.20
R5378:Trim25	UTSW	11	88,900,093 (GRCm39)	missense	probably damaging	1.00
R6149:Trim25	UTSW	11	88,906,362 (GRCm39)	missense	probably benign	0.00
R6867:Trim25	UTSW	11	88,901,713 (GRCm39)	missense	probably benign	0.00
R6996:Trim25	UTSW	11	88,890,329 (GRCm39)	missense	probably benign	0.00
R7055:Trim25	UTSW	11	88,890,750 (GRCm39)	missense	probably benign
R7310:Trim25	UTSW	11	88,906,608 (GRCm39)	missense	probably benign	0.03
R7451:Trim25	UTSW	11	88,906,563 (GRCm39)	missense	possibly damaging	0.76
R7632:Trim25	UTSW	11	88,906,602 (GRCm39)	missense	probably null	0.91
R7767:Trim25	UTSW	11	88,899,943 (GRCm39)	critical splice acceptor site	probably null
R8132:Trim25	UTSW	11	88,907,432 (GRCm39)	missense	probably damaging	0.99
R8785:Trim25	UTSW	11	88,904,340 (GRCm39)	missense	probably benign	0.00
R9135:Trim25	UTSW	11	88,899,988 (GRCm39)	missense	probably benign
R9189:Trim25	UTSW	11	88,901,731 (GRCm39)	missense	probably benign	0.00
R9348:Trim25	UTSW	11	88,900,167 (GRCm39)	nonsense	probably null
R9667:Trim25	UTSW	11	88,907,188 (GRCm39)	missense	probably damaging	1.00
R9731:Trim25	UTSW	11	88,906,391 (GRCm39)	missense	probably benign	0.00
X0022:Trim25	UTSW	11	88,906,422 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CCATTGGGGAGTAGGTAGCTAG -3'
(R):5'- CCACCTCCCAGTAGTGAATG -3'

Sequencing Primer
(F):5'- GAGTAAGTGTTCTGATAGATGAATGG -3'
(R):5'- CCCAGTAGTGAATGCCGTTC -3'

Posted On

2021-10-11