Mutagenetix > Incidental Mutation

Incidental Mutation 'R8997:Zfp873'

684780

Institutional Source

Beutler Lab

Gene Symbol

Zfp873

Ensembl Gene

ENSMUSG00000061371

Gene Name

zinc finger protein 873

Synonyms

MMRRC Submission

068828-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R8997 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

81883957-81900579 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 81896990 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 574 (T574A)

Ref Sequence

ENSEMBL: ENSMUSP00000100950 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105313] [ENSMUST00000209622] [ENSMUST00000210325]

AlphaFold

A0A1B0GT64

Predicted Effect

probably benign
Transcript: ENSMUST00000105313
AA Change: T574A

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000100950
Gene: ENSMUSG00000061371
AA Change: T574A

Domain	Start	End	E-Value	Type
ZnF_C2H2	68	90	1.12e2	SMART
ZnF_C2H2	96	117	4.69e0	SMART
ZnF_C2H2	123	145	2.06e1	SMART
ZnF_C2H2	151	173	5.5e-3	SMART
ZnF_C2H2	179	201	3.69e-4	SMART
ZnF_C2H2	207	229	3.89e-3	SMART
ZnF_C2H2	235	257	9.88e-5	SMART
ZnF_C2H2	263	285	5.59e-4	SMART
ZnF_C2H2	291	313	2.99e-4	SMART
ZnF_C2H2	319	341	1.95e-3	SMART
ZnF_C2H2	347	369	2.75e-3	SMART
ZnF_C2H2	375	397	7.37e-4	SMART
ZnF_C2H2	403	425	2.53e-2	SMART
ZnF_C2H2	431	453	9.08e-4	SMART
ZnF_C2H2	459	481	5.99e-4	SMART
ZnF_C2H2	487	509	1.22e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.53e-2	SMART
ZnF_C2H2	571	593	9.73e-4	SMART
ZnF_C2H2	599	619	3.13e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000209622

Predicted Effect

probably benign
Transcript: ENSMUST00000210325
AA Change: T611A

PolyPhen 2 Score 0.187 (Sensitivity: 0.92; Specificity: 0.87)

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 98.1%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	G	T	1: 11,615,491 (GRCm39)	E155*	probably null	Het
Abhd17a	T	C	10: 80,422,470 (GRCm39)	T71A	probably benign	Het
Adgrv1	GTT	GT	13: 81,553,457 (GRCm39)		probably null	Het
Adra2a	T	C	19: 54,035,729 (GRCm39)	S362P	probably benign	Het
Arid1a	T	A	4: 133,421,343 (GRCm39)	N436Y	unknown	Het
Cma2	T	A	14: 56,210,201 (GRCm39)	C85S	probably benign	Het
Cntn1	G	A	15: 92,132,347 (GRCm39)	V148M	probably damaging	Het
Cntn3	A	G	6: 102,181,023 (GRCm39)	V724A	probably damaging	Het
Dennd1a	C	T	2: 37,690,497 (GRCm39)	G868R	probably benign	Het
Disp2	C	T	2: 118,617,467 (GRCm39)	P154S	probably damaging	Het
Dlgap1	A	G	17: 70,823,528 (GRCm39)	D171G	possibly damaging	Het
Dpp4	A	C	2: 62,164,958 (GRCm39)	I697S	probably damaging	Het
Dync2h1	A	T	9: 7,129,003 (GRCm39)	D1837E	probably benign	Het
Fbxo39	T	A	11: 72,208,466 (GRCm39)	S273T	probably damaging	Het
Fgf5	A	T	5: 98,423,411 (GRCm39)	*265C	probably null	Het
Fscb	A	G	12: 64,520,758 (GRCm39)	V236A	possibly damaging	Het
Greb1l	A	T	18: 10,510,747 (GRCm39)	T614S	probably damaging	Het
Gtdc1	A	T	2: 44,715,386 (GRCm39)	N52K	probably benign	Het
Il6ra	T	C	3: 89,794,418 (GRCm39)	D181G	probably damaging	Het
Inhba	T	G	13: 16,201,107 (GRCm39)	V223G	possibly damaging	Het
Kcnma1	T	C	14: 23,513,037 (GRCm39)		probably benign	Het
Kdm2b	C	A	5: 123,018,236 (GRCm39)	W1068L	probably null	Het
Mdn1	T	C	4: 32,773,275 (GRCm39)	L5485P	probably damaging	Het
Muc16	T	C	9: 18,518,763 (GRCm39)		probably benign	Het
Ncapd3	T	C	9: 26,959,577 (GRCm39)	F338L	probably damaging	Het
Negr1	T	A	3: 156,721,918 (GRCm39)	I164N	probably damaging	Het
Nek9	A	T	12: 85,367,565 (GRCm39)	C369S	probably benign	Het
Ninl	C	T	2: 150,801,816 (GRCm39)	E151K	probably damaging	Het
Nphp1	A	T	2: 127,595,982 (GRCm39)	S494R	possibly damaging	Het
Nphp4	G	A	4: 152,623,345 (GRCm39)	R673H	probably damaging	Het
Obscn	C	T	11: 58,934,629 (GRCm39)		probably benign	Het
Osbpl8	T	C	10: 111,091,575 (GRCm39)	S82P	probably benign	Het
Pcdhgb1	C	A	18: 37,814,133 (GRCm39)	P208Q	probably damaging	Het
Pik3c2b	A	G	1: 133,018,517 (GRCm39)	E988G	possibly damaging	Het
Rfc1	A	T	5: 65,433,064 (GRCm39)	M722K	probably damaging	Het
Rnf111	A	T	9: 70,383,545 (GRCm39)	N129K	probably damaging	Het
Sdccag8	C	A	1: 176,783,374 (GRCm39)	L628I	probably damaging	Het
Sgcz	G	T	8: 39,127,894 (GRCm39)	D8E	probably benign	Het
Sgip1	T	A	4: 102,790,781 (GRCm39)	F254L		Het
Shf	A	G	2: 122,187,728 (GRCm39)	L26P	probably damaging	Het
Slc39a5	G	A	10: 128,232,348 (GRCm39)	A484V	probably damaging	Het
Slco4c1	T	A	1: 96,795,672 (GRCm39)	I129F	probably damaging	Het
Tbck	A	T	3: 132,440,106 (GRCm39)		probably null	Het
Tcstv7b	A	T	13: 120,702,515 (GRCm39)	M104L	probably benign	Het
Teddm3	A	T	16: 20,971,687 (GRCm39)	V294E	probably damaging	Het
Tent4b	T	A	8: 88,979,023 (GRCm39)	V508D	probably benign	Het
Treml1	A	T	17: 48,667,466 (GRCm39)	H117L	probably damaging	Het
Trip12	G	T	1: 84,771,596 (GRCm39)	T184N	probably benign	Het
Tuba3b	G	T	6: 145,566,954 (GRCm39)	M398I	possibly damaging	Het
Vcam1	T	C	3: 115,910,977 (GRCm39)	I474V	probably benign	Het
Wdsub1	A	C	2: 59,688,977 (GRCm39)	M415R	probably damaging	Het
Zfp14	A	T	7: 29,737,600 (GRCm39)	F462I	probably damaging	Het
Zfp846	G	A	9: 20,505,726 (GRCm39)	V529I	probably benign	Het

Other mutations in Zfp873

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02250:Zfp873	APN	10	81,894,252 (GRCm39)	start codon destroyed	probably null	0.45
G1Funyon:Zfp873	UTSW	10	81,896,713 (GRCm39)	missense	probably damaging	1.00
R0666:Zfp873	UTSW	10	81,896,595 (GRCm39)	missense	possibly damaging	0.75
R1568:Zfp873	UTSW	10	81,896,113 (GRCm39)	missense	probably damaging	1.00
R1739:Zfp873	UTSW	10	81,896,541 (GRCm39)	missense	probably damaging	1.00
R1848:Zfp873	UTSW	10	81,896,406 (GRCm39)	missense	probably benign	0.33
R1892:Zfp873	UTSW	10	81,897,080 (GRCm39)	missense	probably damaging	1.00
R2061:Zfp873	UTSW	10	81,895,991 (GRCm39)	missense	probably benign	0.01
R3735:Zfp873	UTSW	10	81,897,015 (GRCm39)	missense	probably benign	0.15
R4422:Zfp873	UTSW	10	81,896,708 (GRCm39)	missense	probably benign	0.13
R4674:Zfp873	UTSW	10	81,895,814 (GRCm39)	missense	possibly damaging	0.53
R4839:Zfp873	UTSW	10	81,896,353 (GRCm39)	missense	probably damaging	0.98
R5146:Zfp873	UTSW	10	81,896,058 (GRCm39)	missense	probably damaging	1.00
R5154:Zfp873	UTSW	10	81,896,025 (GRCm39)	missense	possibly damaging	0.54
R5160:Zfp873	UTSW	10	81,896,876 (GRCm39)	missense	possibly damaging	0.54
R5811:Zfp873	UTSW	10	81,896,567 (GRCm39)	missense	probably damaging	1.00
R6625:Zfp873	UTSW	10	81,896,138 (GRCm39)	missense	probably damaging	1.00
R6667:Zfp873	UTSW	10	81,896,423 (GRCm39)	missense	probably benign	0.22
R6742:Zfp873	UTSW	10	81,894,256 (GRCm39)	missense	probably damaging	1.00
R6878:Zfp873	UTSW	10	81,896,529 (GRCm39)	missense	probably benign	0.33
R7055:Zfp873	UTSW	10	81,895,832 (GRCm39)	missense	probably damaging	1.00
R7296:Zfp873	UTSW	10	81,897,071 (GRCm39)	missense	probably damaging	1.00
R7381:Zfp873	UTSW	10	81,896,805 (GRCm39)	missense	probably damaging	1.00
R7448:Zfp873	UTSW	10	81,896,461 (GRCm39)	missense	probably damaging	1.00
R7464:Zfp873	UTSW	10	81,896,210 (GRCm39)	missense	possibly damaging	0.51
R7470:Zfp873	UTSW	10	81,895,773 (GRCm39)	missense	probably benign	0.19
R7640:Zfp873	UTSW	10	81,896,109 (GRCm39)	missense	possibly damaging	0.72
R7862:Zfp873	UTSW	10	81,896,109 (GRCm39)	missense	probably benign	0.03
R8017:Zfp873	UTSW	10	81,896,193 (GRCm39)	missense	probably benign	0.01
R8301:Zfp873	UTSW	10	81,896,713 (GRCm39)	missense	probably damaging	1.00
R8765:Zfp873	UTSW	10	81,896,072 (GRCm39)	missense	probably damaging	1.00
R9305:Zfp873	UTSW	10	81,896,514 (GRCm39)	missense	probably benign	0.00
R9462:Zfp873	UTSW	10	81,897,131 (GRCm39)	missense	probably benign	0.31
Z1177:Zfp873	UTSW	10	81,896,999 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCCATGAAAGAAGTCACACTGG -3'
(R):5'- CCATGCCATTCATCTTTGTATCAAG -3'

Sequencing Primer
(F):5'- AGAAGCCCTATGACTGTAATCAG -3'
(R):5'- TTTGTATCAAGCACTGCCACAGG -3'

Posted On

2021-10-11