Mutagenetix > Incidental Mutation

Incidental Mutation 'R9002:Usp32'

685043

Institutional Source

Beutler Lab

Gene Symbol

Usp32

Ensembl Gene

ENSMUSG00000000804

Gene Name

ubiquitin specific peptidase 32

Synonyms

6430526O11Rik, 2900074J03Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9002 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84984442-85140161 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 85053951 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 304 (R304C)

Ref Sequence

ENSEMBL: ENSMUSP00000103710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108075]

AlphaFold

F8VPZ3

Predicted Effect

probably damaging
Transcript: ENSMUST00000108075
AA Change: R304C

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000103710
Gene: ENSMUSG00000000804
AA Change: R304C

Domain	Start	End	E-Value	Type
EFh	232	260	4.66e0	SMART
EFh	268	296	5.8e-1	SMART
Blast:EFh	318	346	5e-7	BLAST
DUSP	389	588	2.32e-16	SMART
Pfam:Ubiquitin_3	628	711	2.4e-9	PFAM
Pfam:UCH	733	1564	2.4e-83	PFAM
Pfam:UCH_1	1202	1547	2.9e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,291,926 (GRCm38)	M1263K	possibly damaging	Het
Abca8b	C	T	11: 109,952,630 (GRCm38)	D985N	probably benign	Het
Ak5	A	T	3: 152,653,454 (GRCm38)	M207K	probably damaging	Het
Akt1	T	C	12: 112,659,614 (GRCm38)	I75V	probably benign	Het
Ank	T	A	15: 27,544,327 (GRCm38)	L58*	probably null	Het
Ap1g1	A	C	8: 109,855,106 (GRCm38)	T666P	probably benign	Het
Ap3b2	A	T	7: 81,467,444 (GRCm38)	S615T	probably benign	Het
Ash1l	G	T	3: 88,981,408 (GRCm38)	R198L	probably benign	Het
Axl	A	T	7: 25,778,678 (GRCm38)	C199S	probably damaging	Het
C1d	T	C	11: 17,262,787 (GRCm38)	L44S	probably damaging	Het
Chst13	G	A	6: 90,309,524 (GRCm38)	P152L	probably damaging	Het
Col4a4	A	G	1: 82,471,311 (GRCm38)	L1186P	probably benign	Het
Ctdsp2	T	A	10: 126,996,192 (GRCm38)	I223N	probably damaging	Het
Eml1	T	A	12: 108,538,179 (GRCm38)	I799N	probably damaging	Het
Fbxw18	G	A	9: 109,690,592 (GRCm38)	T282I	probably damaging	Het
Fmo2	A	T	1: 162,878,078 (GRCm38)	C397*	probably null	Het
Gbp10	C	A	5: 105,221,981 (GRCm38)	V262L	probably benign	Het
Gm11639	A	T	11: 105,029,996 (GRCm38)	D4671V	probably damaging	Het
Gm45871	A	T	18: 90,591,844 (GRCm38)	H402L	probably damaging	Het
Has1	T	C	17: 17,843,650 (GRCm38)	S576G	unknown	Het
Hat1	C	T	2: 71,441,303 (GRCm38)	R407W	probably damaging	Het
Hivep2	G	T	10: 14,132,413 (GRCm38)	R1585L	probably benign	Het
Ifi211	A	G	1: 173,906,328 (GRCm38)	V89A	possibly damaging	Het
Igkv4-59	T	C	6: 69,438,491 (GRCm38)	T27A	possibly damaging	Het
Irf9	T	A	14: 55,607,683 (GRCm38)	N333K	possibly damaging	Het
Jakmip2	C	T	18: 43,582,258 (GRCm38)	V68I	probably benign	Het
Kif1b	T	G	4: 149,191,255 (GRCm38)	I1400L	probably damaging	Het
Kif2b	C	T	11: 91,576,227 (GRCm38)	C410Y	probably benign	Het
Klk1b16	T	C	7: 44,140,765 (GRCm38)	L153P	possibly damaging	Het
Kndc1	C	T	7: 139,927,795 (GRCm38)	S1222F	possibly damaging	Het
Lama5	A	G	2: 180,196,518 (GRCm38)	C855R	probably damaging	Het
Mast3	A	G	8: 70,781,260 (GRCm38)	L947P	probably damaging	Het
Mblac2	C	A	13: 81,711,953 (GRCm38)	A142E	possibly damaging	Het
Mppe1	A	G	18: 67,225,854 (GRCm38)	S348P	possibly damaging	Het
Mroh8	A	C	2: 157,217,019 (GRCm38)	V909G	probably damaging	Het
Mthfd1	C	T	12: 76,303,980 (GRCm38)	T712M	probably benign	Het
Nek10	T	C	14: 14,980,590 (GRCm38)	L982P	probably damaging	Het
Nlrp4b	C	T	7: 10,714,959 (GRCm38)	T363I	probably damaging	Het
Nol10	A	G	12: 17,358,133 (GRCm38)	E120G	probably damaging	Het
Olfml1	T	C	7: 107,590,216 (GRCm38)	S163P	probably damaging	Het
Or2n1c	A	T	17: 38,208,664 (GRCm38)	N140Y	probably benign	Het
Or51a7	A	T	7: 102,965,411 (GRCm38)	I104F	probably damaging	Het
Or6ae1	T	C	7: 140,162,285 (GRCm38)	I222V	probably damaging	Het
Or8b43	T	A	9: 38,448,875 (GRCm38)	M1K	probably null	Het
Or8i2	T	C	2: 87,021,897 (GRCm38)	T216A	probably benign	Het
Pde6a	T	A	18: 61,285,989 (GRCm38)	L812Q	probably damaging	Het
Pdxp	T	A	15: 78,918,259 (GRCm38)	M231K	probably damaging	Het
Pi4ka	A	G	16: 17,299,453 (GRCm38)	L1368P		Het
Ppie	T	C	4: 123,130,551 (GRCm38)	N171S	possibly damaging	Het
Rimbp2	T	C	5: 128,788,292 (GRCm38)	H657R	probably benign	Het
Sarnp	T	A	10: 128,821,973 (GRCm38)		probably null	Het
Serpinb9c	T	C	13: 33,150,346 (GRCm38)	T266A	probably damaging	Het
Srgap3	T	A	6: 112,780,893 (GRCm38)	I218F	possibly damaging	Het
Susd1	C	A	4: 59,324,882 (GRCm38)	W717L	probably benign	Het
Tgfbi	A	G	13: 56,623,589 (GRCm38)	Y88C	probably damaging	Het
Tmc6	A	T	11: 117,770,482 (GRCm38)	F624Y	probably damaging	Het
Tnni2	A	G	7: 142,444,276 (GRCm38)	E172G	probably damaging	Het
Traf3ip1	T	C	1: 91,505,456 (GRCm38)	S316P	probably benign	Het
Tshr	C	A	12: 91,537,774 (GRCm38)	N495K	possibly damaging	Het
Ulk3	C	A	9: 57,593,259 (GRCm38)	A317E	probably damaging	Het
Usp24	T	C	4: 106,418,215 (GRCm38)	V2229A	possibly damaging	Het
Usp40	C	T	1: 88,007,341 (GRCm38)	G28D	probably benign	Het
Vmn1r41	A	G	6: 89,747,127 (GRCm38)	K217E	possibly damaging	Het
Vmn2r73	T	A	7: 85,858,076 (GRCm38)	K676M	probably benign	Het
Vnn1	A	G	10: 23,899,451 (GRCm38)	T200A	possibly damaging	Het
Zc3hav1	A	G	6: 38,325,241 (GRCm38)	L698P	possibly damaging	Het

Other mutations in Usp32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00529:Usp32	APN	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
IGL00701:Usp32	APN	11	85,059,125 (GRCm38)	splice site	probably null
IGL00848:Usp32	APN	11	85,051,181 (GRCm38)	splice site	probably benign
IGL00934:Usp32	APN	11	85,007,076 (GRCm38)	missense	probably damaging	1.00
IGL01019:Usp32	APN	11	85,039,265 (GRCm38)	missense	probably damaging	0.97
IGL01302:Usp32	APN	11	84,988,482 (GRCm38)	missense	probably benign	0.05
IGL01444:Usp32	APN	11	85,059,164 (GRCm38)	missense	probably damaging	0.97
IGL01575:Usp32	APN	11	85,022,802 (GRCm38)	missense	probably damaging	1.00
IGL01981:Usp32	APN	11	85,036,524 (GRCm38)	missense	probably benign	0.02
IGL02118:Usp32	APN	11	85,032,177 (GRCm38)	nonsense	probably null
IGL02159:Usp32	APN	11	85,005,802 (GRCm38)	splice site	probably null
IGL02227:Usp32	APN	11	84,986,481 (GRCm38)	missense	probably damaging	1.00
IGL02363:Usp32	APN	11	85,044,787 (GRCm38)	missense	probably benign	0.01
IGL02524:Usp32	APN	11	85,010,011 (GRCm38)	nonsense	probably null
IGL02613:Usp32	APN	11	85,040,070 (GRCm38)	missense	probably damaging	0.99
IGL02720:Usp32	APN	11	85,006,991 (GRCm38)	critical splice donor site	probably null
IGL02738:Usp32	APN	11	85,083,806 (GRCm38)	missense	probably damaging	1.00
IGL02929:Usp32	APN	11	84,988,372 (GRCm38)	missense	probably benign	0.01
IGL03303:Usp32	APN	11	85,022,832 (GRCm38)	missense	probably damaging	1.00
BB010:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
BB020:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
PIT4812001:Usp32	UTSW	11	85,010,074 (GRCm38)	missense	probably damaging	1.00
R0026:Usp32	UTSW	11	85,032,074 (GRCm38)	missense	possibly damaging	0.48
R0295:Usp32	UTSW	11	85,053,692 (GRCm38)	missense	probably damaging	0.98
R1320:Usp32	UTSW	11	85,017,793 (GRCm38)	missense	probably damaging	0.98
R1712:Usp32	UTSW	11	85,042,580 (GRCm38)	missense	probably benign	0.12
R1922:Usp32	UTSW	11	85,007,004 (GRCm38)	nonsense	probably null
R1973:Usp32	UTSW	11	85,103,931 (GRCm38)	missense	probably benign	0.09
R2010:Usp32	UTSW	11	85,040,004 (GRCm38)	missense	probably damaging	0.98
R2082:Usp32	UTSW	11	85,030,512 (GRCm38)	missense	probably damaging	0.99
R2355:Usp32	UTSW	11	85,005,909 (GRCm38)	missense	probably benign	0.34
R3147:Usp32	UTSW	11	85,029,087 (GRCm38)	missense	probably damaging	1.00
R3160:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3162:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3716:Usp32	UTSW	11	85,042,563 (GRCm38)	missense	probably damaging	1.00
R3816:Usp32	UTSW	11	84,994,384 (GRCm38)	critical splice donor site	probably null
R3870:Usp32	UTSW	11	85,007,055 (GRCm38)	nonsense	probably null
R3871:Usp32	UTSW	11	85,081,156 (GRCm38)	missense	probably null	0.81
R4041:Usp32	UTSW	11	85,017,739 (GRCm38)	missense	probably benign	0.40
R4079:Usp32	UTSW	11	85,039,229 (GRCm38)	missense	probably damaging	0.98
R4332:Usp32	UTSW	11	85,103,978 (GRCm38)	missense	possibly damaging	0.79
R4396:Usp32	UTSW	11	85,053,975 (GRCm38)	missense	probably benign
R4580:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4620:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4744:Usp32	UTSW	11	84,994,393 (GRCm38)	missense	probably damaging	1.00
R4909:Usp32	UTSW	11	85,055,772 (GRCm38)	nonsense	probably null
R5056:Usp32	UTSW	11	85,026,795 (GRCm38)	missense	probably benign	0.07
R5111:Usp32	UTSW	11	85,077,331 (GRCm38)	missense	possibly damaging	0.95
R5213:Usp32	UTSW	11	85,022,259 (GRCm38)	missense	probably damaging	1.00
R5308:Usp32	UTSW	11	85,017,718 (GRCm38)	missense	probably benign	0.12
R5381:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably benign
R5538:Usp32	UTSW	11	85,017,786 (GRCm38)	missense	possibly damaging	0.65
R5659:Usp32	UTSW	11	85,077,414 (GRCm38)	missense	possibly damaging	0.94
R6006:Usp32	UTSW	11	84,992,451 (GRCm38)	critical splice donor site	probably null
R6011:Usp32	UTSW	11	85,032,097 (GRCm38)	missense	possibly damaging	0.70
R6029:Usp32	UTSW	11	85,025,582 (GRCm38)	missense	probably damaging	0.99
R6074:Usp32	UTSW	11	84,994,573 (GRCm38)	missense	probably benign	0.00
R6331:Usp32	UTSW	11	84,986,576 (GRCm38)	missense	possibly damaging	0.92
R6353:Usp32	UTSW	11	85,022,281 (GRCm38)	missense	probably benign
R6714:Usp32	UTSW	11	85,026,870 (GRCm38)	missense	probably damaging	0.99
R6778:Usp32	UTSW	11	85,025,686 (GRCm38)	missense	probably benign	0.00
R6988:Usp32	UTSW	11	85,010,143 (GRCm38)	missense	probably benign	0.35
R6992:Usp32	UTSW	11	85,032,088 (GRCm38)	missense	probably damaging	0.99
R7182:Usp32	UTSW	11	85,040,170 (GRCm38)	missense	probably benign	0.34
R7186:Usp32	UTSW	11	85,051,234 (GRCm38)	missense	probably benign	0.45
R7198:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7201:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7469:Usp32	UTSW	11	84,988,553 (GRCm38)	missense	possibly damaging	0.94
R7502:Usp32	UTSW	11	85,022,898 (GRCm38)	missense	possibly damaging	0.48
R7513:Usp32	UTSW	11	85,027,112 (GRCm38)	nonsense	probably null
R7629:Usp32	UTSW	11	85,019,855 (GRCm38)	frame shift	probably null
R7703:Usp32	UTSW	11	85,077,327 (GRCm38)	missense	probably damaging	0.99
R7741:Usp32	UTSW	11	84,987,281 (GRCm38)	missense	probably damaging	0.99
R7765:Usp32	UTSW	11	84,994,408 (GRCm38)	missense	probably damaging	1.00
R7933:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
R7973:Usp32	UTSW	11	85,022,808 (GRCm38)	missense	probably damaging	0.99
R7989:Usp32	UTSW	11	85,034,300 (GRCm38)	missense
R7998:Usp32	UTSW	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
R8292:Usp32	UTSW	11	85,077,401 (GRCm38)	missense	probably damaging	0.99
R8305:Usp32	UTSW	11	85,032,185 (GRCm38)	missense	possibly damaging	0.83
R8548:Usp32	UTSW	11	85,017,827 (GRCm38)	missense	possibly damaging	0.52
R8924:Usp32	UTSW	11	85,025,544 (GRCm38)	missense	probably damaging	0.98
R9145:Usp32	UTSW	11	85,022,292 (GRCm38)	missense	probably damaging	1.00
R9209:Usp32	UTSW	11	85,040,012 (GRCm38)	missense	probably damaging	0.98
R9211:Usp32	UTSW	11	85,022,733 (GRCm38)	missense	probably damaging	1.00
R9296:Usp32	UTSW	11	85,017,652 (GRCm38)	missense	probably damaging	1.00
R9310:Usp32	UTSW	11	85,051,202 (GRCm38)	missense	probably benign	0.29
R9417:Usp32	UTSW	11	84,994,543 (GRCm38)	missense	probably damaging	1.00
R9514:Usp32	UTSW	11	85,022,734 (GRCm38)	missense	probably damaging	0.99
R9652:Usp32	UTSW	11	85,030,491 (GRCm38)	missense	probably damaging	0.97
R9723:Usp32	UTSW	11	85,044,710 (GRCm38)	nonsense	probably null
R9757:Usp32	UTSW	11	85,077,329 (GRCm38)	nonsense	probably null
X0028:Usp32	UTSW	11	84,992,606 (GRCm38)	missense	probably benign	0.05
Z1177:Usp32	UTSW	11	84,988,612 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCCAGTCTCACCTTTGTG -3'
(R):5'- GGGTAGCTTCTAATGAAATGCATC -3'

Sequencing Primer
(F):5'- GTGGTATCATGTGCATTCAATATCC -3'
(R):5'- GCTTCTAATGAAATGCATCATAGTTC -3'

Posted On

2021-10-11