Mutagenetix > Incidental Mutation

Incidental Mutation 'R9003:Or2y1b'

685103

Institutional Source

Beutler Lab

Gene Symbol

Or2y1b

Ensembl Gene

ENSMUSG00000100923

Gene Name

olfactory receptor family 2 subfamily Y member 1B

Synonyms

L45, MOR256-55, Olfr10, GA_x6K02T2QP88-6117098-6116163

MMRRC Submission

068833-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R9003 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

49208375-49209310 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 49209155 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 261 (P261S)

Ref Sequence

ENSEMBL: ENSMUSP00000149724 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000187570] [ENSMUST00000215671] [ENSMUST00000217290]

AlphaFold

Q60883

Predicted Effect

SMART Domains

Protein: ENSMUSP00000139744
Gene: ENSMUSG00000100923
AA Change: P261S

Domain	Start	End	E-Value	Type
low complexity region	23	34	N/A	INTRINSIC
Pfam:7TM_GPCR_Srsx	35	222	4.7e-7	PFAM
Pfam:7tm_1	41	289	1.3e-33	PFAM
Pfam:7tm_4	139	282	4.6e-43	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215671

Predicted Effect

possibly damaging
Transcript: ENSMUST00000217290
AA Change: P261S

PolyPhen 2 Score 0.659 (Sensitivity: 0.86; Specificity: 0.91)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	G	A	12: 118,850,013 (GRCm39)	H917Y	possibly damaging	Het
Acot4	A	G	12: 84,089,969 (GRCm39)	K222R	possibly damaging	Het
Akap12	A	G	10: 4,306,744 (GRCm39)	S1290G	probably benign	Het
Arid1a	T	C	4: 133,411,799 (GRCm39)	I1311V	unknown	Het
Asf1b	G	T	8: 84,682,530 (GRCm39)	E25*	probably null	Het
Atp10a	T	A	7: 58,457,203 (GRCm39)	W901R	probably damaging	Het
Aurkc	T	A	7: 6,999,547 (GRCm39)	I18N	probably damaging	Het
Carmil2	A	G	8: 106,423,905 (GRCm39)	T1253A	probably damaging	Het
Ccdc146	T	C	5: 21,508,132 (GRCm39)	T639A	possibly damaging	Het
Cdca4	A	G	12: 112,785,659 (GRCm39)	V23A	probably benign	Het
Chpt1	A	G	10: 88,312,943 (GRCm39)	F290L	probably damaging	Het
Clca3b	C	T	3: 144,533,072 (GRCm39)	W653*	probably null	Het
Coa8	A	G	12: 111,688,189 (GRCm39)	*45W	probably null	Het
Cpb2	A	T	14: 75,479,868 (GRCm39)		probably benign	Het
Cul9	A	G	17: 46,836,001 (GRCm39)	V1215A	possibly damaging	Het
Cxxc4	A	T	3: 133,945,431 (GRCm39)	N4I	unknown	Het
Eps8l1	G	T	7: 4,464,016 (GRCm39)	V47F	possibly damaging	Het
Exoc6	T	C	19: 37,587,097 (GRCm39)	I537T	probably damaging	Het
Fbn2	T	C	18: 58,176,591 (GRCm39)	Y2040C	probably damaging	Het
Gfm2	T	G	13: 97,282,889 (GRCm39)		probably benign	Het
Jak2	A	T	19: 29,254,240 (GRCm39)	M187L	probably benign	Het
Kif5b	A	G	18: 6,224,047 (GRCm39)	V247A	probably benign	Het
Klhl22	T	C	16: 17,589,612 (GRCm39)	V91A	probably damaging	Het
Ldhd	T	C	8: 112,356,894 (GRCm39)	S17G	probably benign	Het
Madd	C	A	2: 90,988,359 (GRCm39)	E1223*	probably null	Het
Mecom	T	C	3: 30,034,639 (GRCm39)	I346V	probably benign	Het
Mthfd1	C	T	12: 76,350,754 (GRCm39)	T712M	probably benign	Het
Nek4	C	T	14: 30,704,471 (GRCm39)	T662M	probably benign	Het
Or10g3b	T	A	14: 52,586,768 (GRCm39)	H245L	probably damaging	Het
Or4c117	T	A	2: 88,956,024 (GRCm39)	Q17L	possibly damaging	Het
Or4k42	A	G	2: 111,320,411 (GRCm39)	F31L	probably benign	Het
Or5ac19	A	T	16: 59,089,263 (GRCm39)	F256I	probably benign	Het
Or8c18	C	T	9: 38,203,343 (GRCm39)	T34I	probably benign	Het
Phc3	G	A	3: 31,020,007 (GRCm39)	T19I	possibly damaging	Het
Pla2g4e	T	A	2: 120,007,282 (GRCm39)	Q472L	probably benign	Het
Plcg2	C	T	8: 118,342,002 (GRCm39)	T1121I		Het
Plin2	T	C	4: 86,580,324 (GRCm39)	T146A	probably benign	Het
Pum1	T	G	4: 130,474,393 (GRCm39)	S488A	probably benign	Het
Robo1	A	G	16: 72,539,002 (GRCm39)		probably benign	Het
Rsbn1	T	C	3: 103,822,188 (GRCm39)	L102P	probably damaging	Het
Sbno2	A	T	10: 79,896,049 (GRCm39)	V939E	probably damaging	Het
Scarf1	A	T	11: 75,406,069 (GRCm39)	T118S	possibly damaging	Het
Sdcbp2	G	A	2: 151,429,113 (GRCm39)	V171M	probably benign	Het
Sh3pxd2b	T	C	11: 32,361,571 (GRCm39)	I261T	probably damaging	Het
Slc4a9	A	G	18: 36,673,787 (GRCm39)		probably null	Het
Smpdl3a	A	G	10: 57,683,977 (GRCm39)	Y245C	probably damaging	Het
Stk39	T	C	2: 68,222,462 (GRCm39)	I201V	probably damaging	Het
Timm44	A	G	8: 4,324,204 (GRCm39)	L25P	possibly damaging	Het
Tinf2	T	C	14: 55,917,859 (GRCm39)	H210R	probably benign	Het
Tmem168	A	T	6: 13,591,446 (GRCm39)	Y457N	probably benign	Het
Topbp1	T	C	9: 103,200,727 (GRCm39)	S587P	probably benign	Het
Tpp1	T	A	7: 105,398,156 (GRCm39)	I336F	probably benign	Het
Ttc28	G	A	5: 111,424,896 (GRCm39)	V1574I	probably benign	Het
Tut4	A	G	4: 108,400,029 (GRCm39)	K1277E	probably damaging	Het
Vmn2r52	A	T	7: 9,905,181 (GRCm39)	D219E	probably benign	Het
Zbtb40	G	T	4: 136,745,904 (GRCm39)	A43E	probably damaging	Het
Zfp970	G	A	2: 177,167,010 (GRCm39)	A195T	probably damaging	Het

Other mutations in Or2y1b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01349:Or2y1b	APN	11	49,209,127 (GRCm39)	nonsense	probably null	0.00
IGL02283:Or2y1b	APN	11	49,209,162 (GRCm39)	missense	probably benign
IGL03010:Or2y1b	APN	11	49,208,973 (GRCm39)	missense	probably damaging	0.99
R0575:Or2y1b	UTSW	11	49,208,880 (GRCm39)	missense	probably damaging	1.00
R1430:Or2y1b	UTSW	11	49,208,928 (GRCm39)	splice site	probably null
R1891:Or2y1b	UTSW	11	49,208,684 (GRCm39)	missense	probably benign	0.00
R2509:Or2y1b	UTSW	11	49,209,048 (GRCm39)	missense	probably damaging	0.97
R2511:Or2y1b	UTSW	11	49,209,048 (GRCm39)	missense	probably damaging	0.97
R4942:Or2y1b	UTSW	11	49,208,375 (GRCm39)	start codon destroyed	probably null	0.99
R5080:Or2y1b	UTSW	11	49,208,914 (GRCm39)	missense	probably benign	0.03
R5413:Or2y1b	UTSW	11	49,209,240 (GRCm39)	missense	probably damaging	1.00
R5843:Or2y1b	UTSW	11	49,209,076 (GRCm39)	missense	probably benign	0.34
R6354:Or2y1b	UTSW	11	49,208,465 (GRCm39)	missense	probably damaging	0.98
R6605:Or2y1b	UTSW	11	49,208,541 (GRCm39)	missense	probably damaging	0.97
R8005:Or2y1b	UTSW	11	49,208,968 (GRCm39)	missense	probably benign	0.12
R8284:Or2y1b	UTSW	11	49,209,002 (GRCm39)	missense	probably benign
R8902:Or2y1b	UTSW	11	49,209,206 (GRCm39)	missense	probably benign	0.03
R9431:Or2y1b	UTSW	11	49,208,459 (GRCm39)	missense	probably benign
R9696:Or2y1b	UTSW	11	49,208,390 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTGGCCAGAGCAGTGATTG -3'
(R):5'- ATTGCCCTTTCCCATGGAAC -3'

Sequencing Primer
(F):5'- CAGTGATTGTTGCAGTGCCTACC -3'
(R):5'- TCCCATGGAACTACTCAGAATATG -3'

Posted On

2021-10-11