Mutagenetix > Incidental Mutation

Incidental Mutation 'R9005:Ptgr2'

685240

Institutional Source

Beutler Lab

Gene Symbol

Ptgr2

Ensembl Gene

ENSMUSG00000072946

Gene Name

prostaglandin reductase 2

Synonyms

Zadh1, 9630002F03Rik, B830026H24Rik, 1810016I24Rik, PGR-2

MMRRC Submission

068835-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R9005 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

84332011-84362606 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 84344873 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 82 (V82A)

Ref Sequence

ENSEMBL: ENSMUSP00000115704 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000123614] [ENSMUST00000123693] [ENSMUST00000135001] [ENSMUST00000146377] [ENSMUST00000147363]

AlphaFold

Q8VDQ1

PDB Structure

Crystal structure of putative NADPH-dependent oxidoreductase from Mus musculus at 2.10 A resolution [X-RAY DIFFRACTION]
Crystal structure of mouse 15-ketoprostaglandin delta-13-reductase in complex with NADPH [X-RAY DIFFRACTION]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000123614
AA Change: V82A

PolyPhen 2 Score 0.859 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000115704
Gene: ENSMUSG00000072946
AA Change: V82A

Domain	Start	End	E-Value	Type
Pfam:ADH_N_2	4	165	5.4e-40	PFAM
Pfam:ADH_zinc_N	166	287	1.3e-15	PFAM
Pfam:ADH_zinc_N_2	200	343	4.5e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000123693

SMART Domains

Protein: ENSMUSP00000115506
Gene: ENSMUSG00000072946

Domain	Start	End	E-Value	Type
Pfam:ADH_N_2	4	56	1.3e-12	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000135001
AA Change: V82A

PolyPhen 2 Score 0.398 (Sensitivity: 0.89; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000114559
Gene: ENSMUSG00000072946
AA Change: V82A

Domain	Start	End	E-Value	Type
Pfam:ADH_zinc_N	166	283	7.4e-19	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000146377
AA Change: V82A

PolyPhen 2 Score 0.859 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000119981
Gene: ENSMUSG00000072946
AA Change: V82A

Domain	Start	End	E-Value	Type
Pfam:ADH_zinc_N	166	283	1.4e-16	PFAM
Pfam:ADH_zinc_N_2	200	343	6.9e-9	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000147363
AA Change: V82A

PolyPhen 2 Score 0.830 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000114766
Gene: ENSMUSG00000072946
AA Change: V82A

Domain	Start	End	E-Value	Type
Pfam:ADH_zinc_N	166	283	1.2e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	C	T	19: 21,630,075 (GRCm39)		probably benign	Het
Abcd4	C	T	12: 84,655,356 (GRCm39)	W369*	probably null	Het
Abcf3	A	G	16: 20,368,056 (GRCm39)	E98G	probably benign	Het
Acaca	A	T	11: 84,262,410 (GRCm39)	D2004V	probably damaging	Het
Acot5	G	C	12: 84,116,630 (GRCm39)	M130I		Het
Adamts3	C	T	5: 89,825,693 (GRCm39)	E1049K	probably benign	Het
Agfg2	A	T	5: 137,650,744 (GRCm39)	L456Q	probably damaging	Het
Agtr1b	T	A	3: 20,370,343 (GRCm39)	T88S	possibly damaging	Het
Amer2	G	C	14: 60,617,376 (GRCm39)	D524H	probably damaging	Het
Ankrd61	T	A	5: 143,831,676 (GRCm39)	T43S	probably benign	Het
Apcs	A	G	1: 172,721,776 (GRCm39)	L190P	probably benign	Het
Arsg	A	G	11: 109,381,346 (GRCm39)	K30E	probably benign	Het
Banp	A	T	8: 122,705,441 (GRCm39)	M104L	possibly damaging	Het
Caskin1	A	G	17: 24,718,111 (GRCm39)	Q268R	probably benign	Het
Ccdc112	A	G	18: 46,429,455 (GRCm39)	V81A	probably damaging	Het
Cfap44	T	C	16: 44,280,517 (GRCm39)	I1432T	probably damaging	Het
Chd2	A	T	7: 73,134,294 (GRCm39)	N684K	probably damaging	Het
Creb1	T	C	1: 64,605,478 (GRCm39)		probably null	Het
Ctsc	C	T	7: 87,927,502 (GRCm39)	T31I	probably damaging	Het
Cttnbp2	C	A	6: 18,434,430 (GRCm39)	R476L	probably damaging	Het
Cwf19l1	T	C	19: 44,111,653 (GRCm39)	D260G	possibly damaging	Het
Cyp24a1	A	T	2: 170,336,005 (GRCm39)	S211T	probably damaging	Het
Ddx3y	T	C	Y: 1,282,919 (GRCm39)	N46D	probably damaging	Het
Dgkz	G	T	2: 91,769,090 (GRCm39)	H653Q	probably benign	Het
Dnajc16	A	T	4: 141,491,945 (GRCm39)	I626N	possibly damaging	Het
Dsc2	T	C	18: 20,171,151 (GRCm39)	D616G	probably benign	Het
Dscam	A	T	16: 96,602,580 (GRCm39)	S621T	probably damaging	Het
Dst	T	A	1: 34,267,774 (GRCm39)	V3058E	probably damaging	Het
Ep400	A	T	5: 110,858,959 (GRCm39)	C1125S	unknown	Het
Glyatl3	G	A	17: 41,223,619 (GRCm39)	Q41*	probably null	Het
Gm7694	T	G	1: 170,128,927 (GRCm39)	I201L	probably damaging	Het
Gnal	C	T	18: 67,221,830 (GRCm39)	R78*	probably null	Het
Gtf3c1	T	C	7: 125,303,069 (GRCm39)	D122G	probably benign	Het
Htt	G	T	5: 34,975,095 (GRCm39)	D622Y	possibly damaging	Het
Igkv4-92	A	G	6: 68,732,081 (GRCm39)	C98R	probably benign	Het
Incenp	G	A	19: 9,855,088 (GRCm39)	R608*	probably null	Het
Kics2	A	G	10: 121,586,501 (GRCm39)	Y272C	probably damaging	Het
Kif3c	G	A	12: 3,451,706 (GRCm39)	R679H	probably damaging	Het
Kng1	T	A	16: 22,898,146 (GRCm39)	H515Q	probably damaging	Het
Lamb2	T	C	9: 108,361,370 (GRCm39)		probably null	Het
Malrd1	A	T	2: 15,850,140 (GRCm39)	N1253I	unknown	Het
Map3k21	A	T	8: 126,637,471 (GRCm39)	D19V		Het
Marchf2	G	T	17: 33,915,207 (GRCm39)	P129Q	probably damaging	Het
Mier2	G	A	10: 79,384,274 (GRCm39)	R166W	probably damaging	Het
Ms4a4d	T	A	19: 11,533,564 (GRCm39)	D148E	possibly damaging	Het
Myl4	A	G	11: 104,468,384 (GRCm39)	D36G	probably damaging	Het
Or1x6	G	A	11: 50,938,938 (GRCm39)	M1I	probably null	Het
Or2a25	T	C	6: 42,889,170 (GRCm39)	S238P	probably damaging	Het
Or5ac24	A	T	16: 59,165,929 (GRCm39)	I45N	probably damaging	Het
Or5an11	A	G	19: 12,245,704 (GRCm39)	T37A	probably benign	Het
Pard3	G	A	8: 128,003,647 (GRCm39)	S123N	probably damaging	Het
Parp8	T	C	13: 117,013,126 (GRCm39)	N638D	probably benign	Het
Pgpep1l	A	T	7: 67,887,406 (GRCm39)	S65T	probably damaging	Het
Ppp1r13b	A	G	12: 111,796,708 (GRCm39)	V1068A	probably benign	Het
Pramel20	G	A	4: 143,298,425 (GRCm39)	D123N	probably benign	Het
Prkd1	G	A	12: 50,430,185 (GRCm39)	Q641*	probably null	Het
Rbm34	G	A	8: 127,686,332 (GRCm39)	P225S	possibly damaging	Het
Rnps1	A	T	17: 24,637,496 (GRCm39)	K46I	unknown	Het
Shank1	T	C	7: 44,002,409 (GRCm39)	V1376A	probably benign	Het
Ski	A	T	4: 155,306,317 (GRCm39)	Y221N	probably damaging	Het
Skint6	C	A	4: 113,095,347 (GRCm39)	G104V	probably damaging	Het
Slit2	G	A	5: 48,459,860 (GRCm39)	C1380Y	possibly damaging	Het
Smpd3	C	T	8: 106,984,058 (GRCm39)	G554S	probably benign	Het
Sorbs2	T	G	8: 46,248,774 (GRCm39)	V675G	probably benign	Het
Spdl1	C	A	11: 34,700,535 (GRCm39)	C605F	possibly damaging	Het
Syne1	C	A	10: 5,155,406 (GRCm39)	D5568Y	probably benign	Het
Tac1	C	A	6: 7,559,141 (GRCm39)	A111D	possibly damaging	Het
Tmco1	C	T	1: 167,136,132 (GRCm39)		probably benign	Het
Tnfrsf1a	T	C	6: 125,333,878 (GRCm39)	C44R	probably damaging	Het
Traf3ip3	T	C	1: 192,864,285 (GRCm39)	Q366R	probably benign	Het
Trip10	A	G	17: 57,569,416 (GRCm39)	D479G	probably damaging	Het
Trip11	T	C	12: 101,845,131 (GRCm39)	T1536A	probably benign	Het
Tstd3	G	T	4: 21,767,082 (GRCm39)	A13E	possibly damaging	Het
Urb1	T	C	16: 90,550,678 (GRCm39)	K2058R	probably benign	Het
Usp15	A	T	10: 122,982,703 (GRCm39)	I304N	possibly damaging	Het
Usp18	T	G	6: 121,229,529 (GRCm39)	H49Q	probably benign	Het
Vmn1r56	T	A	7: 5,199,389 (GRCm39)	D76V	probably damaging	Het
Wnk1	A	G	6: 119,939,393 (GRCm39)	S167P	probably damaging	Het
Zfp229	G	A	17: 21,965,731 (GRCm39)	A654T	possibly damaging	Het
Zfp977	C	T	7: 42,230,082 (GRCm39)	V148I	probably benign	Het
Znfx1	A	T	2: 166,880,656 (GRCm39)	I1240N		Het

Other mutations in Ptgr2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02382:Ptgr2	APN	12	84,360,722 (GRCm39)	missense	probably damaging	0.99
IGL03176:Ptgr2	APN	12	84,354,668 (GRCm39)	missense	probably damaging	1.00
R1914:Ptgr2	UTSW	12	84,349,096 (GRCm39)	missense	probably benign	0.11
R1915:Ptgr2	UTSW	12	84,349,096 (GRCm39)	missense	probably benign	0.11
R4624:Ptgr2	UTSW	12	84,355,128 (GRCm39)	missense	possibly damaging	0.70
R5827:Ptgr2	UTSW	12	84,342,110 (GRCm39)	critical splice donor site	probably null
R5978:Ptgr2	UTSW	12	84,342,032 (GRCm39)	nonsense	probably null
R5986:Ptgr2	UTSW	12	84,355,120 (GRCm39)	missense	possibly damaging	0.85
R6019:Ptgr2	UTSW	12	84,344,920 (GRCm39)	missense	probably damaging	1.00
R6320:Ptgr2	UTSW	12	84,349,111 (GRCm39)	missense	probably benign	0.05
R6526:Ptgr2	UTSW	12	84,360,726 (GRCm39)	missense	probably damaging	1.00
R7369:Ptgr2	UTSW	12	84,339,080 (GRCm39)	start gained	probably benign
R7401:Ptgr2	UTSW	12	84,339,103 (GRCm39)	start gained	probably benign
R7463:Ptgr2	UTSW	12	84,339,072 (GRCm39)	start gained	probably benign
R7583:Ptgr2	UTSW	12	84,355,179 (GRCm39)	missense	probably damaging	1.00
R7793:Ptgr2	UTSW	12	84,354,575 (GRCm39)	missense	probably damaging	1.00
R8172:Ptgr2	UTSW	12	84,360,783 (GRCm39)	missense	possibly damaging	0.93
R9495:Ptgr2	UTSW	12	84,354,647 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAAGCAAACTAGCAGTACATGTG -3'
(R):5'- GGCTTTCCACATTTTGGCAC -3'

Sequencing Primer
(F):5'- CTTGCCTTAGTGAGACCT -3'
(R):5'- TCCACATTTTGGCACTGTTATATG -3'

Posted On

2021-10-11