Mutagenetix > Incidental Mutation

Incidental Mutation 'R9009:Scn9a'

685484

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

068839-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9009 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66310424-66465306 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 66338927 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 1186 (I1186M)

Ref Sequence

ENSEMBL: ENSMUSP00000097642 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

probably damaging
Transcript: ENSMUST00000100063
AA Change: I1177M

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: I1177M

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100064
AA Change: I1186M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: I1186M

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000112354
AA Change: I1175M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: I1175M

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164384
AA Change: I1186M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: I1186M

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000169900
AA Change: I1175M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: I1175M

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

94% (58/62)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	T	A	8: 120,878,279 (GRCm39)		probably benign	Het
Ahctf1	C	T	1: 179,581,171 (GRCm39)	S1677N	probably benign	Het
Ank2	T	C	3: 126,728,025 (GRCm39)		probably benign	Het
Arid3c	A	T	4: 41,729,925 (GRCm39)	I90N	probably benign	Het
Asb1	C	A	1: 91,480,205 (GRCm39)	D301E	unknown	Het
Asb1	T	G	1: 91,480,206 (GRCm39)	*302G	probably null	Het
Birc2	A	T	9: 7,833,937 (GRCm39)	F181L	probably benign	Het
Bnip1	T	A	17: 27,001,590 (GRCm39)		probably benign	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,128,173 (GRCm39)		probably benign	Het
Cd2ap	T	C	17: 43,116,135 (GRCm39)	D575G	possibly damaging	Het
Cdca7	T	A	2: 72,314,273 (GRCm39)	H248Q	probably damaging	Het
Chd2	T	C	7: 73,143,192 (GRCm39)	D567G	probably benign	Het
Chd2	T	C	7: 73,140,402 (GRCm39)	I609V	probably benign	Het
Cnmd	T	A	14: 79,894,085 (GRCm39)	T101S	probably damaging	Het
Col6a4	T	C	9: 105,954,404 (GRCm39)	T312A	probably benign	Het
Cript	C	A	17: 87,338,475 (GRCm39)	T41N	probably damaging	Het
Cts6	T	C	13: 61,344,261 (GRCm39)	I264V	probably benign	Het
Cyp4a32	C	T	4: 115,467,802 (GRCm39)	T262I	probably null	Het
D430041D05Rik	T	A	2: 104,240,521 (GRCm39)		probably benign	Het
Dctd	C	T	8: 48,564,712 (GRCm39)	P5S	probably benign	Het
Det1	T	C	7: 78,492,984 (GRCm39)	N340S	probably benign	Het
Dnah7b	A	G	1: 46,262,232 (GRCm39)	N2032D	probably benign	Het
Dscam	T	A	16: 96,840,116 (GRCm39)	T164S	probably benign	Het
Emcn	T	C	3: 137,124,775 (GRCm39)	V196A	possibly damaging	Het
Fbxo32	A	G	15: 58,046,358 (GRCm39)	I284T	possibly damaging	Het
Gimap3	C	A	6: 48,742,094 (GRCm39)	D279Y	possibly damaging	Het
Gm43302	C	T	5: 105,427,974 (GRCm39)	D196N	probably benign	Het
Gpc6	C	A	14: 117,424,217 (GRCm39)	H102N	possibly damaging	Het
Gpr158	A	T	2: 21,581,760 (GRCm39)	D413V	probably damaging	Het
Ifi207	T	C	1: 173,555,382 (GRCm39)	R767G	probably damaging	Het
Kcnip2	T	C	19: 45,800,634 (GRCm39)		probably benign	Het
Kifap3	A	C	1: 163,696,291 (GRCm39)	D640A	probably damaging	Het
Lmbrd2	A	T	15: 9,157,311 (GRCm39)	Q183L	possibly damaging	Het
Malt1	A	G	18: 65,577,911 (GRCm39)	I135V	probably benign	Het
Mfhas1	T	C	8: 36,057,109 (GRCm39)	V528A	probably damaging	Het
Mllt10	T	C	2: 18,167,163 (GRCm39)	S363P	probably damaging	Het
Mrnip	A	G	11: 50,073,323 (GRCm39)	Y44C	probably damaging	Het
Mstn	C	T	1: 53,103,131 (GRCm39)	Q156*	probably null	Het
Mtmr7	T	C	8: 41,008,904 (GRCm39)	D469G	possibly damaging	Het
Muc6	A	G	7: 141,217,018 (GRCm39)	S2552P	possibly damaging	Het
Myo1f	C	A	17: 33,823,662 (GRCm39)	N1063K	probably benign	Het
Or52k2	T	A	7: 102,253,642 (GRCm39)	I27N	probably benign	Het
Or5af1	A	T	11: 58,722,280 (GRCm39)	Y100F	probably benign	Het
Or6z5	G	A	7: 6,477,399 (GRCm39)	V97M	probably benign	Het
Orai2	A	G	5: 136,179,430 (GRCm39)	V201A	possibly damaging	Het
Pcdhga6	A	T	18: 37,841,878 (GRCm39)	T533S	possibly damaging	Het
Pkd1l1	T	A	11: 8,881,552 (GRCm39)	K907N		Het
Plaat5	G	T	19: 7,614,823 (GRCm39)	M228I	probably benign	Het
Ppp1r15a	A	T	7: 45,174,049 (GRCm39)	V253E	probably benign	Het
Ppp1r9b	A	G	11: 94,887,467 (GRCm39)	E493G	probably benign	Het
Prdm1	T	C	10: 44,322,997 (GRCm39)	N151S	probably benign	Het
Prkcd	C	T	14: 30,329,297 (GRCm39)	A163T	probably damaging	Het
Prl7a2	T	A	13: 27,849,994 (GRCm39)	E26V	probably damaging	Het
Ptprm	A	T	17: 66,996,354 (GRCm39)	N1278K	probably damaging	Het
Ptprz1	T	A	6: 23,001,653 (GRCm39)	S1248T	possibly damaging	Het
Sv2a	T	C	3: 96,094,409 (GRCm39)	F248S	probably benign	Het
Tas2r116	T	A	6: 132,832,963 (GRCm39)	I188K	probably damaging	Het
Tcf21	T	C	10: 22,693,671 (GRCm39)	T169A	probably benign	Het
Tecrl	C	A	5: 83,432,121 (GRCm39)	C258F	probably damaging	Het
Tet2	T	A	3: 133,193,360 (GRCm39)	E358V	possibly damaging	Het
Thada	A	T	17: 84,759,203 (GRCm39)	S219T	possibly damaging	Het
Tpgs2	T	A	18: 25,301,777 (GRCm39)		probably benign	Het
Ttc6	A	T	12: 57,744,219 (GRCm39)	I1284F	probably damaging	Het
Vmn1r160	T	C	7: 22,571,127 (GRCm39)	V160A	possibly damaging	Het
Vmn1r44	T	C	6: 89,870,671 (GRCm39)	L139P	probably damaging	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,393,945 (GRCm39)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,314,486 (GRCm39)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,314,279 (GRCm39)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,314,645 (GRCm39)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,357,316 (GRCm39)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,318,397 (GRCm39)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,357,416 (GRCm39)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,367,722 (GRCm39)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,324,230 (GRCm39)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,317,986 (GRCm39)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,314,386 (GRCm39)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,314,777 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,325,170 (GRCm39)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,377,479 (GRCm39)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,323,447 (GRCm39)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,314,955 (GRCm39)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,366,440 (GRCm39)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,367,637 (GRCm39)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,370,903 (GRCm39)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,392,855 (GRCm39)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,314,358 (GRCm39)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,357,091 (GRCm39)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,392,788 (GRCm39)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,363,437 (GRCm39)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,398,475 (GRCm39)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,335,354 (GRCm39)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,373,621 (GRCm39)	missense	probably benign
R0465:Scn9a	UTSW	2	66,357,340 (GRCm39)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,314,022 (GRCm39)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,357,143 (GRCm39)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,367,721 (GRCm39)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,363,405 (GRCm39)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,363,721 (GRCm39)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,313,843 (GRCm39)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,377,456 (GRCm39)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,366,468 (GRCm39)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,314,079 (GRCm39)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,335,341 (GRCm39)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,393,886 (GRCm39)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,314,930 (GRCm39)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,357,232 (GRCm39)	missense	probably benign
R1511:Scn9a	UTSW	2	66,357,157 (GRCm39)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,335,371 (GRCm39)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,314,648 (GRCm39)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,318,361 (GRCm39)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,313,803 (GRCm39)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,335,220 (GRCm39)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,313,850 (GRCm39)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,317,938 (GRCm39)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,332,060 (GRCm39)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,396,594 (GRCm39)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,314,655 (GRCm39)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,345,724 (GRCm39)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,345,665 (GRCm39)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,363,720 (GRCm39)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,398,527 (GRCm39)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,314,396 (GRCm39)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,356,998 (GRCm39)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,366,669 (GRCm39)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,364,573 (GRCm39)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,335,312 (GRCm39)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,392,749 (GRCm39)	missense	probably benign
R3771:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,313,992 (GRCm39)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,357,217 (GRCm39)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,395,398 (GRCm39)	intron	probably benign
R4385:Scn9a	UTSW	2	66,314,900 (GRCm39)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,357,037 (GRCm39)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,313,902 (GRCm39)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,377,362 (GRCm39)	missense	probably benign
R4783:Scn9a	UTSW	2	66,370,967 (GRCm39)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,314,093 (GRCm39)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,382,057 (GRCm39)	missense	probably benign
R4908:Scn9a	UTSW	2	66,357,087 (GRCm39)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,396,614 (GRCm39)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,392,824 (GRCm39)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,364,463 (GRCm39)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,395,511 (GRCm39)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,318,387 (GRCm39)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,377,447 (GRCm39)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,395,373 (GRCm39)	intron	probably benign
R6108:Scn9a	UTSW	2	66,314,393 (GRCm39)	missense	probably damaging	1.00
R6115:Scn9a	UTSW	2	66,393,973 (GRCm39)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,317,868 (GRCm39)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,314,240 (GRCm39)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,398,608 (GRCm39)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,357,307 (GRCm39)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,313,846 (GRCm39)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,393,686 (GRCm39)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,398,373 (GRCm39)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,379,359 (GRCm39)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,364,567 (GRCm39)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,314,748 (GRCm39)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,314,931 (GRCm39)	missense	probably benign
R7386:Scn9a	UTSW	2	66,370,894 (GRCm39)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,377,531 (GRCm39)	missense	possibly damaging	0.81
R7483:Scn9a	UTSW	2	66,363,692 (GRCm39)	missense	probably damaging	0.99
R7485:Scn9a	UTSW	2	66,364,561 (GRCm39)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,313,990 (GRCm39)	missense	probably benign
R7617:Scn9a	UTSW	2	66,370,893 (GRCm39)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,366,580 (GRCm39)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,357,424 (GRCm39)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,314,642 (GRCm39)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,314,135 (GRCm39)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,314,904 (GRCm39)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,373,456 (GRCm39)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,335,193 (GRCm39)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,314,597 (GRCm39)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,345,774 (GRCm39)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,317,754 (GRCm39)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,314,745 (GRCm39)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,370,874 (GRCm39)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,366,626 (GRCm39)	missense	probably benign
R8345:Scn9a	UTSW	2	66,324,966 (GRCm39)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,396,625 (GRCm39)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,332,015 (GRCm39)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,366,628 (GRCm39)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,332,010 (GRCm39)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,370,979 (GRCm39)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,313,961 (GRCm39)	missense	probably benign
R9038:Scn9a	UTSW	2	66,325,147 (GRCm39)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,314,744 (GRCm39)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,363,657 (GRCm39)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,335,236 (GRCm39)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,314,261 (GRCm39)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,357,040 (GRCm39)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,395,553 (GRCm39)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,314,328 (GRCm39)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,363,708 (GRCm39)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,392,809 (GRCm39)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,357,002 (GRCm39)	missense	probably benign
X0003:Scn9a	UTSW	2	66,338,991 (GRCm39)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,398,421 (GRCm39)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,370,936 (GRCm39)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,325,029 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GAACCATGTTTATGATAGCCTACC -3'
(R):5'- GAAGGTCCTGCAGCATTAGG -3'

Sequencing Primer
(F):5'- TGATAGCCTACCAATTAACCTTCAG -3'
(R):5'- CGATGTAATGTGGGAGAAGGTAGTCC -3'

Posted On

2021-10-11