Mutagenetix > Incidental Mutation

Incidental Mutation 'R9018:Muc4'

686172

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

068848-MU

Accession Numbers

Genbank: NM_080457; MGI: 2153525

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R9018 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

32735886-32782391 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 32762536 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 492 (Y492H)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096106
AA Change: Y2562H

PolyPhen 2 Score 0.931 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: Y2562H

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620
AA Change: Y492H

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

100% (77/77)

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	A	G	7: 120,319,309 (GRCm38)	E1552G	probably damaging	Het
Abca14	A	T	7: 120,289,540 (GRCm38)	K1236N	probably benign	Het
Adam3	A	T	8: 24,694,276 (GRCm38)	Y569*	probably null	Het
Adgrb2	A	G	4: 130,013,866 (GRCm38)	T998A	probably benign	Het
Adgre4	T	A	17: 55,791,993 (GRCm38)	H166Q	probably benign	Het
Ank1	G	A	8: 23,116,248 (GRCm38)	G1219S	probably null	Het
Ankrd11	A	G	8: 122,895,512 (GRCm38)	S534P	probably damaging	Het
Atn1	C	T	6: 124,745,698 (GRCm38)	E805K	unknown	Het
Bsn	T	C	9: 108,117,289 (GRCm38)	T596A	probably benign	Het
Cacnb4	C	T	2: 52,434,694 (GRCm38)	R452Q	probably benign	Het
Cdc123	C	A	2: 5,844,872 (GRCm38)	A13S	probably benign	Het
Chd7	G	A	4: 8,847,083 (GRCm38)	G1609S	possibly damaging	Het
Cp	T	A	3: 19,989,152 (GRCm38)	C1035S	probably damaging	Het
Dcaf1	T	A	9: 106,865,637 (GRCm38)	C1383S	probably damaging	Het
Derl3	G	A	10: 75,893,770 (GRCm38)	V54I	probably benign	Het
Dgcr6	T	C	16: 18,066,743 (GRCm38)	L25P	probably damaging	Het
Dmrt2	A	G	19: 25,673,621 (GRCm38)	E57G	probably benign	Het
Dst	A	G	1: 34,196,059 (GRCm38)	K3562E	probably damaging	Het
Dyrk2	T	C	10: 118,860,109 (GRCm38)	T415A	probably damaging	Het
Fam186b	G	A	15: 99,279,735 (GRCm38)	A570V	probably damaging	Het
Fbln1	T	C	15: 85,242,014 (GRCm38)	I484T	probably damaging	Het
Greb1l	T	A	18: 10,542,004 (GRCm38)	D1250E	possibly damaging	Het
Ift88	A	C	14: 57,438,245 (GRCm38)	K72Q	probably benign	Het
Impg1	T	A	9: 80,394,192 (GRCm38)	I228F	probably benign	Het
Itsn2	A	G	12: 4,658,091 (GRCm38)	N799S	possibly damaging	Het
Jade1	T	A	3: 41,609,857 (GRCm38)	C521S	probably benign	Het
Katna1	T	G	10: 7,761,276 (GRCm38)	L397R	probably damaging	Het
Kcnj15	A	C	16: 95,296,270 (GRCm38)	K250N	probably damaging	Het
Lrrc6	A	T	15: 66,449,630 (GRCm38)	S221T	probably benign	Het
Macc1	A	G	12: 119,446,206 (GRCm38)	I236M	possibly damaging	Het
Mapk13	C	T	17: 28,777,786 (GRCm38)	R276C	probably benign	Het
Mier2	G	A	10: 79,548,440 (GRCm38)	R166W	probably damaging	Het
Mindy4	C	T	6: 55,301,087 (GRCm38)	H639Y	possibly damaging	Het
Mphosph9	A	G	5: 124,298,650 (GRCm38)	S544P	probably benign	Het
Mybbp1a	C	T	11: 72,443,594 (GRCm38)	T225I	probably benign	Het
Myo6	A	T	9: 80,251,804 (GRCm38)	K285I	unknown	Het
Nmd3	G	A	3: 69,739,995 (GRCm38)	V277I	probably benign	Het
Nol9	G	C	4: 152,039,461 (GRCm38)	R36P	probably damaging	Het
Nom1	A	G	5: 29,434,714 (GRCm38)	R13G	possibly damaging	Het
Nudt14	G	A	12: 112,939,286 (GRCm38)	H40Y	probably damaging	Het
Nxt2	C	T	X: 142,237,751 (GRCm38)	A118V	possibly damaging	Het
Olfr1375	G	A	11: 51,048,111 (GRCm38)	M1I	probably null	Het
Olfr1474	A	T	19: 13,471,357 (GRCm38)	H87L	possibly damaging	Het
Olfr933	T	A	9: 38,976,391 (GRCm38)	F238L	probably benign	Het
Pcnx4	T	A	12: 72,556,663 (GRCm38)	F492I	probably damaging	Het
Pex19	GTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTC	GTCTCTTGTCTCCGAAGGTGCTCTTGATGATTTC	1: 172,128,583 (GRCm38)		probably null	Het
Pgghg	A	G	7: 140,944,666 (GRCm38)	I309V	probably benign	Het
Phf12	C	T	11: 78,023,684 (GRCm38)	P651S	possibly damaging	Het
Pltp	A	G	2: 164,852,490 (GRCm38)	L199P	probably damaging	Het
Pole	T	A	5: 110,289,809 (GRCm38)	L78I	probably benign	Het
Ppm1d	C	A	11: 85,337,135 (GRCm38)	H292Q	probably damaging	Het
Ppp2cb	A	C	8: 33,615,759 (GRCm38)	I224L	probably benign	Het
Rabggta	A	T	14: 55,720,423 (GRCm38)	I171N	probably damaging	Het
Rapgef5	A	G	12: 117,748,397 (GRCm38)	I740V	probably damaging	Het
Rb1cc1	G	A	1: 6,249,266 (GRCm38)	E970K	probably benign	Het
Rnh1	A	T	7: 141,168,631 (GRCm38)	V11D	probably benign	Het
Robo4	C	T	9: 37,404,224 (GRCm38)	T288I	probably benign	Het
Scmh1	A	G	4: 120,505,317 (GRCm38)	D250G	probably benign	Het
Sele	T	A	1: 164,053,679 (GRCm38)	C483S	probably damaging	Het
Slc12a6	A	G	2: 112,344,240 (GRCm38)		probably benign	Het
Slc5a4a	A	G	10: 76,166,712 (GRCm38)	E234G	possibly damaging	Het
Smarca5	A	G	8: 80,704,726 (GRCm38)	L954P	probably damaging	Het
St7	T	A	6: 17,906,495 (GRCm38)	N413K	probably damaging	Het
Stam2	C	T	2: 52,716,451 (GRCm38)	V141I	probably benign	Het
Stim1	C	T	7: 102,411,275 (GRCm38)	T175I	probably benign	Het
Strap	A	G	6: 137,739,813 (GRCm38)	N130S	probably benign	Het
Stxbp2	A	G	8: 3,642,627 (GRCm38)		probably benign	Het
Sult3a2	T	A	10: 33,779,693 (GRCm38)	I97F	probably benign	Het
Tbc1d32	A	T	10: 56,072,597 (GRCm38)	N965K	probably benign	Het
Tle3	T	A	9: 61,412,468 (GRCm38)	I506N	probably damaging	Het
Tmco1	C	T	1: 167,308,563 (GRCm38)		probably benign	Het
Tvp23a	A	G	16: 10,446,982 (GRCm38)	S22P	probably damaging	Het
Vmn2r82	A	G	10: 79,396,705 (GRCm38)	N846S	probably damaging	Het
Vmn2r83	A	G	10: 79,480,186 (GRCm38)	N472S	probably damaging	Het
Xpo7	G	A	14: 70,707,424 (GRCm38)	Q10*	probably null	Het
Zfp423	G	C	8: 87,781,753 (GRCm38)	S654R	probably benign	Het
Zfp646	A	T	7: 127,879,071 (GRCm38)	H140L	probably benign	Het
Zfp719	C	A	7: 43,584,065 (GRCm38)		probably benign	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,778,812 (GRCm38)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,753,185 (GRCm38)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,750,910 (GRCm38)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,754,347 (GRCm38)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,849 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,848 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,823 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,755,805 (GRCm38)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,753,955 (GRCm38)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,754,071 (GRCm38)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,763,966 (GRCm38)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,750,655 (GRCm38)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,756,627 (GRCm38)	missense	unknown
IGL01625:Muc4	APN	16	32,755,544 (GRCm38)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,736,402 (GRCm38)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,761,348 (GRCm38)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,753,196 (GRCm38)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,751,426 (GRCm38)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,754,265 (GRCm38)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,777,649 (GRCm38)	splice site	probably benign
IGL02210:Muc4	APN	16	32,752,254 (GRCm38)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,750,850 (GRCm38)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,752,076 (GRCm38)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,756,129 (GRCm38)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,775,819 (GRCm38)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,755,282 (GRCm38)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,751,648 (GRCm38)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,750,394 (GRCm38)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,752,048 (GRCm38)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,751,948 (GRCm38)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,751,439 (GRCm38)	nonsense	probably null
IGL03335:Muc4	APN	16	32,753,021 (GRCm38)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,754,318 (GRCm38)	missense	probably benign	0.01
multisplendored	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,770,394 (GRCm38)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,760,251 (GRCm38)	splice site	probably benign
BB004:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
BB014:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
IGL02835:Muc4	UTSW	16	32,763,945 (GRCm38)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,760,248 (GRCm38)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,754,796 (GRCm38)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,756,017 (GRCm38)	missense	unknown
R0119:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,771,604 (GRCm38)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,765,746 (GRCm38)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,752,905 (GRCm38)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,762,536 (GRCm38)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,751,069 (GRCm38)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,756,746 (GRCm38)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,752,107 (GRCm38)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,751,670 (GRCm38)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,769,827 (GRCm38)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,769,847 (GRCm38)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,778,955 (GRCm38)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,756,196 (GRCm38)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,750,525 (GRCm38)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,753,020 (GRCm38)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,752,233 (GRCm38)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,750,349 (GRCm38)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,755,501 (GRCm38)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,756,902 (GRCm38)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,755,933 (GRCm38)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,756,251 (GRCm38)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,756,341 (GRCm38)	missense	unknown
R1928:Muc4	UTSW	16	32,750,532 (GRCm38)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,750,642 (GRCm38)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,751,303 (GRCm38)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,752,254 (GRCm38)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,756,409 (GRCm38)	missense	unknown
R2121:Muc4	UTSW	16	32,760,238 (GRCm38)	missense	unknown
R2139:Muc4	UTSW	16	32,761,225 (GRCm38)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,750,476 (GRCm38)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,766,942 (GRCm38)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,755,891 (GRCm38)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,757,472 (GRCm38)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,755,273 (GRCm38)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,755,826 (GRCm38)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,770,292 (GRCm38)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,756,701 (GRCm38)	missense	unknown
R4523:Muc4	UTSW	16	32,736,336 (GRCm38)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,755,843 (GRCm38)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,757,058 (GRCm38)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,769,277 (GRCm38)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,755,846 (GRCm38)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,751,214 (GRCm38)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,775,903 (GRCm38)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,753,625 (GRCm38)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,751,747 (GRCm38)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,756,098 (GRCm38)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,754,319 (GRCm38)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,041 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,756,296 (GRCm38)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,757,058 (GRCm38)	nonsense	probably null
R5161:Muc4	UTSW	16	32,762,521 (GRCm38)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,751,738 (GRCm38)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,751,666 (GRCm38)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,761,261 (GRCm38)	missense	unknown
R5567:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,750,782 (GRCm38)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,776,807 (GRCm38)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,736,241 (GRCm38)	nonsense	probably null
R5708:Muc4	UTSW	16	32,754,769 (GRCm38)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,751,916 (GRCm38)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,774,839 (GRCm38)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,751,295 (GRCm38)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,751,705 (GRCm38)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,753,052 (GRCm38)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,756,278 (GRCm38)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,759,308 (GRCm38)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,775,783 (GRCm38)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,756,795 (GRCm38)	missense	unknown
R6144:Muc4	UTSW	16	32,766,924 (GRCm38)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,736,140 (GRCm38)	start gained	probably benign
R6268:Muc4	UTSW	16	32,768,767 (GRCm38)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,752,035 (GRCm38)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,753,946 (GRCm38)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,754,358 (GRCm38)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,767,351 (GRCm38)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,736,243 (GRCm38)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,778,946 (GRCm38)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,751,789 (GRCm38)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,759,320 (GRCm38)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,782,008 (GRCm38)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,766,938 (GRCm38)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,775,803 (GRCm38)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,762,518 (GRCm38)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,751,999 (GRCm38)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,751,810 (GRCm38)	nonsense	probably null
R7033:Muc4	UTSW	16	32,756,324 (GRCm38)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,757,091 (GRCm38)	missense
R7123:Muc4	UTSW	16	32,750,691 (GRCm38)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,762,488 (GRCm38)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,756,461 (GRCm38)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,755,336 (GRCm38)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,755,366 (GRCm38)	missense	unknown
R7381:Muc4	UTSW	16	32,780,915 (GRCm38)	missense
R7411:Muc4	UTSW	16	32,751,322 (GRCm38)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,766,950 (GRCm38)	missense
R7539:Muc4	UTSW	16	32,756,396 (GRCm38)	missense
R7544:Muc4	UTSW	16	32,736,198 (GRCm38)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,765,702 (GRCm38)	missense
R7596:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,752,361 (GRCm38)	nonsense	probably null
R7639:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,760,105 (GRCm38)	missense
R7651:Muc4	UTSW	16	32,756,575 (GRCm38)	missense
R7688:Muc4	UTSW	16	32,751,460 (GRCm38)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,753,011 (GRCm38)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,768,734 (GRCm38)	missense
R7763:Muc4	UTSW	16	32,753,311 (GRCm38)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,756,194 (GRCm38)	missense	unknown
R7787:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,751,321 (GRCm38)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
R7930:Muc4	UTSW	16	32,754,078 (GRCm38)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,756,749 (GRCm38)	missense
R8096:Muc4	UTSW	16	32,755,390 (GRCm38)	missense	unknown
R8115:Muc4	UTSW	16	32,755,304 (GRCm38)	missense	unknown
R8162:Muc4	UTSW	16	32,750,379 (GRCm38)	missense	unknown
R8220:Muc4	UTSW	16	32,755,327 (GRCm38)	missense	unknown
R8260:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,754,316 (GRCm38)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,755,897 (GRCm38)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,752,401 (GRCm38)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,753,508 (GRCm38)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,752,993 (GRCm38)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,755,255 (GRCm38)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,781,986 (GRCm38)	missense
R8845:Muc4	UTSW	16	32,756,515 (GRCm38)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,750,643 (GRCm38)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,751,462 (GRCm38)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,753,994 (GRCm38)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,752,209 (GRCm38)	missense	probably benign	0.01
R9034:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,757,468 (GRCm38)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,768,728 (GRCm38)	missense
R9336:Muc4	UTSW	16	32,750,938 (GRCm38)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,751,153 (GRCm38)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,756,618 (GRCm38)	missense
R9429:Muc4	UTSW	16	32,755,724 (GRCm38)	missense	unknown
R9570:Muc4	UTSW	16	32,750,877 (GRCm38)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,756,860 (GRCm38)	missense
R9696:Muc4	UTSW	16	32,753,284 (GRCm38)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,770,270 (GRCm38)	missense
R9743:Muc4	UTSW	16	32,780,824 (GRCm38)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,751,858 (GRCm38)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,751,825 (GRCm38)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,755,481 (GRCm38)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,759,319 (GRCm38)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,756,076 (GRCm38)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,767,393 (GRCm38)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,774,862 (GRCm38)	missense
Z1177:Muc4	UTSW	16	32,767,394 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- AATAGGTGACGTCCCTGTGTC -3'
(R):5'- TCAAAGGTTAAAGGACTCCGGTAC -3'

Sequencing Primer
(F):5'- GTCCCTGTGTCTGCTCAGAG -3'
(R):5'- ACTCCGGTACACAAGTGTTG -3'

Posted On

2021-10-11