Mutagenetix > Incidental Mutation

Incidental Mutation 'R9027:Vmn1r228'

686834

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r228

Ensembl Gene

ENSMUSG00000060245

Gene Name

vomeronasal 1 receptor 228

Synonyms

V1re3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R9027 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20776059-20777501 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20777160 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 32 (D32G)

Ref Sequence

ENSEMBL: ENSMUSP00000072243 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072410]

AlphaFold

Q8R2A7

Predicted Effect

probably benign
Transcript: ENSMUST00000072410
AA Change: D32G

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000072243
Gene: ENSMUSG00000060245
AA Change: D32G

Domain	Start	End	E-Value	Type
Pfam:TAS2R	32	317	2.6e-11	PFAM
Pfam:7tm_1	53	316	2.6e-9	PFAM
Pfam:V1R	63	321	1.3e-26	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (59/60)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017D01Rik	A	T	19: 11,105,691	Y122N	probably damaging	Het
Adam1b	C	A	5: 121,502,725	E86*	probably null	Het
Ahnak	T	G	19: 9,007,253	I1967S	possibly damaging	Het
Alox12e	A	G	11: 70,321,774	V83A	possibly damaging	Het
Arhgap20	A	T	9: 51,843,677	R439S	probably damaging	Het
Arl1	A	G	10: 88,733,596	I20V	probably damaging	Het
Atad2	T	C	15: 58,132,232	D93G	probably benign	Het
Btbd7	T	G	12: 102,838,579	K67N	probably damaging	Het
C130050O18Rik	A	G	5: 139,414,546	N118S	probably benign	Het
Ccz1	A	C	5: 144,009,302		probably benign	Het
Cdk19	A	G	10: 40,479,732	S479G	unknown	Het
Chrd	A	G	16: 20,736,987	T503A	probably damaging	Het
Clca4b	G	A	3: 144,912,066	R759*	probably null	Het
Cpa5	G	T	6: 30,612,605	M1I	probably null	Het
Cr2	A	T	1: 195,151,721	I920N	probably benign	Het
Crmp1	C	A	5: 37,280,603	Y430*	probably null	Het
Dars	A	T	1: 128,368,426	V390D	possibly damaging	Het
Dmtn	A	G	14: 70,616,115	S85P	probably damaging	Het
Fancm	C	A	12: 65,075,831	D42E	probably damaging	Het
Gabrg3	T	A	7: 56,773,374	Y192F	possibly damaging	Het
Gm3435	A	G	17: 15,022,102	E416G	unknown	Het
Gm8765	C	T	13: 50,702,971	Q882*	probably null	Het
Gpr3	A	T	4: 133,210,898	Y154*	probably null	Het
Huwe1	G	A	X: 151,933,088	R4331Q	unknown	Het
Ints5	C	A	19: 8,895,958	P427Q	possibly damaging	Het
Jhy	A	T	9: 40,917,527	V361D	probably benign	Het
Klhl33	G	A	14: 50,892,865	Q131*	probably null	Het
Lama2	A	T	10: 27,204,885	C981S	probably damaging	Het
Mks1	C	A	11: 87,857,215	L225I	probably damaging	Het
Nfasc	T	A	1: 132,611,605	S402C	probably damaging	Het
Olfr1300-ps1	A	T	2: 111,692,172	Y218F	unknown	Het
Olfr47	T	C	6: 43,236,424	I272T	possibly damaging	Het
Olfr577	T	C	7: 102,973,353	D213G	probably damaging	Het
Pbx4	T	A	8: 69,864,349	D85E	possibly damaging	Het
Plcd1	T	C	9: 119,084,641	T50A	probably damaging	Het
Plk5	C	G	10: 80,357,996	R40G	probably damaging	Het
Psen2	C	A	1: 180,229,407	E351*	probably null	Het
Rragd	G	A	4: 32,996,083	V143I	probably damaging	Het
Rsph14	A	T	10: 74,959,591	M254K	probably damaging	Het
Selenoi	C	T	5: 30,232,609		probably benign	Het
Six6	T	C	12: 72,940,161	S36P		Het
Slc12a8	T	C	16: 33,624,845	S370P	probably benign	Het
Slc25a46	A	G	18: 31,583,379	Y357H	probably benign	Het
Socs2	A	T	10: 95,413,086	V55D	probably damaging	Het
Socs6	T	C	18: 88,870,728	E21G	probably benign	Het
Speg	A	T	1: 75,388,432	T486S	possibly damaging	Het
Spryd3	A	G	15: 102,119,408	Y235H	probably damaging	Het
Sry	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	Y: 2,662,638		probably benign	Het
Stxbp5l	T	C	16: 37,345,111	K82E	probably damaging	Het
Sugt1	A	G	14: 79,587,715		probably benign	Het
Synm	T	C	7: 67,734,692	Y1074C	probably damaging	Het
Sytl2	A	G	7: 90,379,540	T476A	probably benign	Het
Tbc1d1	T	C	5: 64,257,006	S237P	probably benign	Het
Tbc1d5	C	A	17: 50,756,664	M629I	probably damaging	Het
Tlr11	G	A	14: 50,361,292	G245D	probably damaging	Het
Tspan4	T	C	7: 141,489,664	V59A	probably benign	Het
Tulp4	T	A	17: 6,233,197	V1167E	possibly damaging	Het
Usp42	G	A	5: 143,723,151	T204M	probably damaging	Het
Vapb	A	G	2: 173,776,155	K147R	possibly damaging	Het
Vmn2r33	A	G	7: 7,551,169	F795S	probably damaging	Het
Vmn2r34	A	T	7: 7,672,528	N620K	probably damaging	Het
Vwf	G	T	6: 125,666,663	C2389F		Het
Wdr81	T	C	11: 75,442,082	E652G		Het
Wdr81	A	T	11: 75,452,381	S687T	probably benign	Het
Zcrb1	A	G	15: 93,387,575		probably null	Het

Other mutations in Vmn1r228

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01368:Vmn1r228	APN	17	20776512	missense	probably benign	0.07
IGL03275:Vmn1r228	APN	17	20776842	missense	probably damaging	1.00
PIT4498001:Vmn1r228	UTSW	17	20776510	missense	probably benign	0.00
R0097:Vmn1r228	UTSW	17	20776363	missense	probably benign	0.05
R0097:Vmn1r228	UTSW	17	20776363	missense	probably benign	0.05
R0270:Vmn1r228	UTSW	17	20776596	missense	possibly damaging	0.60
R0279:Vmn1r228	UTSW	17	20776375	missense	probably benign	0.02
R1544:Vmn1r228	UTSW	17	20777023	missense	probably benign	0.00
R1695:Vmn1r228	UTSW	17	20776298	missense	possibly damaging	0.49
R2086:Vmn1r228	UTSW	17	20777193	missense	possibly damaging	0.71
R2275:Vmn1r228	UTSW	17	20776545	missense	probably damaging	1.00
R2965:Vmn1r228	UTSW	17	20776347	missense	probably damaging	0.99
R4425:Vmn1r228	UTSW	17	20776599	missense	probably damaging	1.00
R4447:Vmn1r228	UTSW	17	20777107	missense	probably damaging	0.96
R5031:Vmn1r228	UTSW	17	20776681	nonsense	probably null
R6345:Vmn1r228	UTSW	17	20776882	missense	probably damaging	1.00
R7064:Vmn1r228	UTSW	17	20777023	missense	probably benign	0.00
R7880:Vmn1r228	UTSW	17	20776410	missense	probably damaging	1.00
R8000:Vmn1r228	UTSW	17	20776965	missense	possibly damaging	0.88
R8290:Vmn1r228	UTSW	17	20776462	missense	probably benign	0.09
R9022:Vmn1r228	UTSW	17	20776516	missense	probably damaging	1.00
R9291:Vmn1r228	UTSW	17	20776761	missense	probably benign
R9492:Vmn1r228	UTSW	17	20776600	missense	probably damaging	1.00
R9618:Vmn1r228	UTSW	17	20776783	missense	probably benign	0.00
X0018:Vmn1r228	UTSW	17	20776701	missense	probably benign	0.08

Predicted Primers

PCR Primer
(F):5'- GGGTCCTTTGGAGAGAAGGATC -3'
(R):5'- ACATGTGCACTCAGACTTTGAAAG -3'

Sequencing Primer
(F):5'- TCAAGGCGTTGGATGTAAACAC -3'
(R):5'- GCACTCAGACTTTGAAAGTTTCATGG -3'

Posted On

2021-11-19