Mutagenetix > Incidental Mutation

Incidental Mutation 'R9043:Cyp2g1'

687836

Institutional Source

Beutler Lab

Gene Symbol

Cyp2g1

Ensembl Gene

ENSMUSG00000049685

Gene Name

cytochrome P450, family 2, subfamily g, polypeptide 1

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9043 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

26808892-26821205 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 26809831 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 110 (N110S)

Ref Sequence

ENSEMBL: ENSMUSP00000047150 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040944]

AlphaFold

Q9WV19

Predicted Effect

probably benign
Transcript: ENSMUST00000040944
AA Change: N110S

PolyPhen 2 Score 0.048 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000047150
Gene: ENSMUSG00000049685
AA Change: N110S

Domain	Start	End	E-Value	Type
low complexity region	12	23	N/A	INTRINSIC
Pfam:p450	34	491	4e-150	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.0%

Validation Efficiency

99% (87/88)

MGI Phenotype

PHENOTYPE: Mice homozygous for disruptions in this gene display an essentially normal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402J07Rik	T	A	8: 87,582,610	S134T	possibly damaging	Het
Aadacl4	T	A	4: 144,623,220	L349Q	probably damaging	Het
Ankrd29	T	C	18: 12,295,913	N33S	probably damaging	Het
Arid3b	T	C	9: 57,792,617	S536G	possibly damaging	Het
Art5	A	C	7: 102,099,492	M1R	probably null	Het
Arvcf	T	A	16: 18,399,702	C545S	probably damaging	Het
Axin2	T	A	11: 108,942,968	N580K	probably benign	Het
BC037034	G	A	5: 138,261,627	T389I	possibly damaging	Het
Cdh23	T	G	10: 60,315,699	D2469A	probably benign	Het
Cep350	T	A	1: 155,897,482	T1781S	probably damaging	Het
Cfap65	T	A	1: 74,904,688	T1555S	possibly damaging	Het
Chga	G	A	12: 102,562,795	E344K	possibly damaging	Het
Ckap2l	A	T	2: 129,284,972	L429M	probably damaging	Het
Cpne7	C	T	8: 123,130,212	P402L	probably damaging	Het
Cyp3a11	T	C	5: 145,869,185	D124G	probably benign	Het
Dgkq	A	T	5: 108,653,195	V527D	probably damaging	Het
Dnah3	T	C	7: 119,952,049	I37V	probably benign	Het
Dnah9	T	A	11: 65,954,854	E3068V		Het
Dusp23	T	C	1: 172,632,617	E89G	probably null	Het
Fam173b	A	G	15: 31,616,955	T189A	probably benign	Het
Fam187a	A	G	11: 102,885,859	Y163C	possibly damaging	Het
Fam19a2	T	C	10: 123,704,389	V50A	probably damaging	Het
Gabrg2	T	A	11: 41,974,835	I106F	probably damaging	Het
Gpc2	A	G	5: 138,278,931	I78T	probably benign	Het
Gpr149	A	C	3: 62,603,939	L213R	probably damaging	Het
Grm1	A	T	10: 10,689,312	L1084*	probably null	Het
Gypa	A	G	8: 80,496,317	T17A	unknown	Het
Gys2	A	T	6: 142,430,333	F534L	probably damaging	Het
Iqce	A	T	5: 140,666,055	M721K	probably benign	Het
Klrc2	T	A	6: 129,658,778	Y129F	probably damaging	Het
Krt14	T	C	11: 100,204,638	S314G	possibly damaging	Het
L3mbtl3	T	C	10: 26,280,254	Q685R	unknown	Het
Lamb3	A	T	1: 193,325,611	K206*	probably null	Het
Lgr5	A	G	10: 115,478,438	I198T	probably damaging	Het
Mapk10	A	T	5: 102,926,159		probably benign	Het
Mcm6	C	T	1: 128,343,494	D463N	probably damaging	Het
Med15	A	T	16: 17,652,718	W742R	probably benign	Het
Meis1	T	A	11: 18,881,916	Q374L	possibly damaging	Het
Nrxn3	T	C	12: 89,260,482	C296R	probably damaging	Het
Oaf	C	T	9: 43,224,011	A157T	probably damaging	Het
Oca2	T	A	7: 56,277,441		probably null	Het
Olfr1163	T	A	2: 88,070,639	T248S	possibly damaging	Het
Olfr1448	T	A	19: 12,920,303	E2V	probably benign	Het
Olfr1464-ps1	A	T	19: 13,282,710	M116K	probably damaging	Het
Olfr826	A	T	10: 130,180,285	H198Q	probably benign	Het
Pacs1	A	G	19: 5,138,936	I806T	probably benign	Het
Pax2	T	A	19: 44,816,060	S257T	probably benign	Het
Pbxip1	A	T	3: 89,443,487	T72S	probably benign	Het
Pcdhb14	T	C	18: 37,448,798	I319T	probably damaging	Het
Pcdhga2	C	A	18: 37,669,910	A269E	possibly damaging	Het
Pdzph1	A	C	17: 58,973,540	N582K	probably benign	Het
Plk2	A	T	13: 110,396,660	I186F	probably damaging	Het
Pzp	A	T	6: 128,494,779	C895S	probably damaging	Het
Rasa2	A	T	9: 96,602,717	V148D	probably damaging	Het
Rgsl1	A	T	1: 153,841,821	I14N	possibly damaging	Het
Rhox3c	C	T	X: 37,469,650		probably benign	Het
Rnf213	A	G	11: 119,458,913	R3867G		Het
Rpap3	A	G	15: 97,686,562	Y334H	possibly damaging	Het
Scara3	A	C	14: 65,931,621	N182K	probably damaging	Het
Sh3bp1	A	G	15: 78,907,249	E412G	possibly damaging	Het
Sh3tc2	T	A	18: 61,989,890	I574N	possibly damaging	Het
Slc16a11	T	C	11: 70,215,694	Y253H	probably damaging	Het
Slc38a4	A	T	15: 97,008,924	V311E	possibly damaging	Het
Slc4a7	T	C	14: 14,775,048	L826P	probably damaging	Het
Spag9	T	G	11: 94,060,259	V83G		Het
Sspo	T	G	6: 48,493,280	V4560G	probably benign	Het
Stxbp2	T	A	8: 3,634,478	F144I		Het
Tdh	T	C	14: 63,492,829	I334V	probably benign	Het
Tesk2	C	A	4: 116,802,951	S451Y	probably benign	Het
Tjp1	A	T	7: 65,312,931	V1166E	probably benign	Het
Tmeff2	G	A	1: 50,979,620	V254M	unknown	Het
Tmem126a	A	G	7: 90,451,541		probably null	Het
Togaram2	C	G	17: 71,686,704	P2A	probably benign	Het
Tssk4	C	T	14: 55,651,754	R246W	probably damaging	Het
Tubal3	C	T	13: 3,932,708	R163*	probably null	Het
Usp48	G	A	4: 137,613,685	G332E	probably benign	Het
Vezt	T	C	10: 93,984,165	N446S	probably damaging	Het
Vim	A	T	2: 13,574,438	T3S	unknown	Het
Vmn2r45	A	G	7: 8,471,905	L708P	possibly damaging	Het
Wdyhv1	A	G	15: 58,150,608	Y67C	possibly damaging	Het
Yes1	A	G	5: 32,652,968	Y157C	probably damaging	Het
Zfp358	A	G	8: 3,495,810	T131A	probably benign	Het
Zfp426	C	T	9: 20,475,012	V58M	probably damaging	Het
Zfp692	T	C	11: 58,307,724	S47P	probably damaging	Het
Zfp719	A	T	7: 43,590,181	T398S	possibly damaging	Het

Other mutations in Cyp2g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01134:Cyp2g1	APN	7	26809831	missense	probably benign	0.05
IGL01137:Cyp2g1	APN	7	26814259	missense	possibly damaging	0.87
IGL02052:Cyp2g1	APN	7	26814294	splice site	probably benign
IGL02338:Cyp2g1	APN	7	26814804	splice site	probably benign
IGL02452:Cyp2g1	APN	7	26811446	missense	probably benign	0.28
IGL02523:Cyp2g1	APN	7	26819187	missense	probably damaging	1.00
IGL03165:Cyp2g1	APN	7	26809776	missense	possibly damaging	0.94
IGL03230:Cyp2g1	APN	7	26819403	missense	probably damaging	1.00
PIT4472001:Cyp2g1	UTSW	7	26814194	missense	probably benign	0.28
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0106:Cyp2g1	UTSW	7	26814182	missense	probably damaging	1.00
R0380:Cyp2g1	UTSW	7	26814295	splice site	probably benign
R0697:Cyp2g1	UTSW	7	26814727	nonsense	probably null
R0830:Cyp2g1	UTSW	7	26814791	missense	probably benign	0.00
R1660:Cyp2g1	UTSW	7	26809682	critical splice acceptor site	probably null
R2093:Cyp2g1	UTSW	7	26819433	missense	probably benign	0.35
R2131:Cyp2g1	UTSW	7	26820710	missense	probably damaging	0.99
R4606:Cyp2g1	UTSW	7	26814154	missense	possibly damaging	0.80
R5030:Cyp2g1	UTSW	7	26820801	missense	probably benign	0.06
R5574:Cyp2g1	UTSW	7	26820740	missense	possibly damaging	0.81
R5877:Cyp2g1	UTSW	7	26816640	missense	possibly damaging	0.80
R6745:Cyp2g1	UTSW	7	26814179	missense	probably damaging	1.00
R7040:Cyp2g1	UTSW	7	26820759	missense	probably damaging	0.99
R7223:Cyp2g1	UTSW	7	26814632	missense	probably damaging	0.98
R7934:Cyp2g1	UTSW	7	26819193	missense	probably damaging	1.00
R8112:Cyp2g1	UTSW	7	26819461	missense	probably benign
R8177:Cyp2g1	UTSW	7	26819153	missense	probably damaging	1.00
R8194:Cyp2g1	UTSW	7	26814734	missense	possibly damaging	0.89
R9406:Cyp2g1	UTSW	7	26819485	critical splice donor site	probably null
R9441:Cyp2g1	UTSW	7	26814635	missense	possibly damaging	0.72
X0067:Cyp2g1	UTSW	7	26820762	missense	possibly damaging	0.70

Predicted Primers

PCR Primer
(F):5'- ACACAGCTCTGTCATGCTCC -3'
(R):5'- CTGCCCTCAAACATACTGTGC -3'

Sequencing Primer
(F):5'- ACAGCTCTGTCATGCTCCAATTTAC -3'
(R):5'- ACTGTGCAACACTATCTAACCTTTC -3'

Posted On

2021-11-19