Mutagenetix > Incidental Mutation

Incidental Mutation 'R9046:Or5b101'

688084

Institutional Source

Beutler Lab

Gene Symbol

Or5b101

Ensembl Gene

ENSMUSG00000094755

Gene Name

olfactory receptor family 5 subfamily B member 101

Synonyms

Olfr1453, GA_x6K02T2RE5P-3357666-3356743, MOR202-6

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R9046 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

13004768-13005691 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 13005115 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 193 (N193D)

Ref Sequence

ENSEMBL: ENSMUSP00000085071 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087773]

AlphaFold

Q8VFX0

Predicted Effect

probably benign
Transcript: ENSMUST00000087773
AA Change: N193D

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000085071
Gene: ENSMUSG00000094755
AA Change: N193D

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	6.6e-50	PFAM
Pfam:7tm_1	39	288	2.6e-20	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,243,525 (GRCm39)	V1796A	probably benign	Het
Abca3	A	G	17: 24,617,477 (GRCm39)	D1058G	probably damaging	Het
Adgrg6	A	G	10: 14,323,858 (GRCm39)	V431A	probably benign	Het
Amdhd1	C	T	10: 93,363,087 (GRCm39)	D334N	probably damaging	Het
Ankrd50	A	T	3: 38,506,642 (GRCm39)	M252K	probably benign	Het
Anxa3	G	A	5: 96,976,626 (GRCm39)	R204Q	probably damaging	Het
Apmap	T	C	2: 150,426,093 (GRCm39)	T352A	probably benign	Het
Arhgef4	A	G	1: 34,850,846 (GRCm39)	E464G	possibly damaging	Het
Atp11b	T	A	3: 35,852,740 (GRCm39)		probably benign	Het
Brwd1	T	A	16: 95,829,402 (GRCm39)	S1100C	probably damaging	Het
Ccm2l	T	A	2: 152,916,720 (GRCm39)	I317N	probably damaging	Het
Ccr9	C	T	9: 123,608,831 (GRCm39)	T171I	probably benign	Het
Cdh23	A	T	10: 60,218,303 (GRCm39)		probably benign	Het
Dact1	A	G	12: 71,365,534 (GRCm39)	I735V	probably benign	Het
Dok6	C	A	18: 89,787,221 (GRCm39)	V14L	probably benign	Het
Dthd1	A	T	5: 62,984,603 (GRCm39)	Y436F	possibly damaging	Het
Fndc9	G	T	11: 46,128,889 (GRCm39)	W136L	probably damaging	Het
Gal3st1	G	A	11: 3,948,278 (GRCm39)	V162I	probably benign	Het
Gart	A	G	16: 91,418,561 (GRCm39)	S973P	probably damaging	Het
H2-T5	A	T	17: 36,476,035 (GRCm39)		probably null	Het
Hsp90ab1	G	A	17: 45,879,969 (GRCm39)	P516S	probably damaging	Het
Htr1a	G	C	13: 105,581,816 (GRCm39)	G352A	probably damaging	Het
Hycc2	T	C	1: 58,568,945 (GRCm39)	T545A	probably damaging	Het
Isg20	A	T	7: 78,569,823 (GRCm39)	R265*	probably null	Het
Kcmf1	C	T	6: 72,825,455 (GRCm39)	A213T	probably damaging	Het
Kdm3a	A	G	6: 71,572,540 (GRCm39)	V1007A	probably damaging	Het
Klhl25	A	G	7: 75,515,337 (GRCm39)	E81G	probably damaging	Het
Klhl6	C	A	16: 19,765,803 (GRCm39)	V600F	probably damaging	Het
Kmt2b	A	G	7: 30,285,479 (GRCm39)	V471A	probably benign	Het
Lap3	A	G	5: 45,652,562 (GRCm39)	T53A	probably damaging	Het
Lmntd1	A	T	6: 145,365,565 (GRCm39)	I188N	probably damaging	Het
Lrba	A	G	3: 86,302,543 (GRCm39)	I1868V	possibly damaging	Het
Mef2d	A	T	3: 88,074,825 (GRCm39)	Y337F	probably benign	Het
Mgat4e	T	C	1: 134,474,742 (GRCm39)	T7A	possibly damaging	Het
Myo3a	G	T	2: 22,448,367 (GRCm39)	L985F	probably damaging	Het
Myof	T	C	19: 37,923,112 (GRCm39)		probably benign	Het
Ncapg2	G	A	12: 116,376,145 (GRCm39)	G77S	probably benign	Het
Nostrin	G	T	2: 68,975,123 (GRCm39)	V13L	probably benign	Het
Nup155	TGGG	TGG	15: 8,157,919 (GRCm39)		probably null	Het
Or14j6	A	G	17: 38,215,145 (GRCm39)	K236R	probably damaging	Het
Or3a10	T	C	11: 73,935,284 (GRCm39)	D272G	probably damaging	Het
Or4a75	C	T	2: 89,448,496 (GRCm39)		probably benign	Het
Parp4	A	C	14: 56,864,927 (GRCm39)	K1025T	probably damaging	Het
Parvb	T	C	15: 84,174,639 (GRCm39)	I144T	probably benign	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Phf10	A	G	17: 15,175,160 (GRCm39)	V195A	probably damaging	Het
Prpf18	A	T	2: 4,640,464 (GRCm39)	M257K	possibly damaging	Het
Ptcd3	A	T	6: 71,870,364 (GRCm39)		probably null	Het
Ptprt	G	A	2: 161,372,361 (GRCm39)	T1437M	possibly damaging	Het
Ptx3	T	C	3: 66,132,153 (GRCm39)	F225L	probably damaging	Het
Rpl3l	A	G	17: 24,947,435 (GRCm39)	K50E	probably damaging	Het
Rubcn	T	C	16: 32,661,940 (GRCm39)	N427D	probably benign	Het
Serpinb8	G	A	1: 107,530,563 (GRCm39)	A114T	possibly damaging	Het
Sgsm1	A	G	5: 113,436,725 (GRCm39)	V35A	probably damaging	Het
Slc22a2	G	A	17: 12,834,234 (GRCm39)	A501T	probably null	Het
Slu7	G	A	11: 43,335,629 (GRCm39)	C455Y	probably damaging	Het
Src	C	A	2: 157,307,795 (GRCm39)	H235N	probably damaging	Het
Svopl	T	A	6: 37,998,531 (GRCm39)	M248L	probably benign	Het
Tbx6	G	A	7: 126,381,120 (GRCm39)		probably null	Het
Tprg1l	A	T	4: 154,242,913 (GRCm39)	I239N	probably damaging	Het
Trafd1	A	G	5: 121,513,189 (GRCm39)	F350L	probably benign	Het
Trpv3	A	G	11: 73,176,698 (GRCm39)	Y359C	probably damaging	Het
Vmn1r46	T	C	6: 89,953,585 (GRCm39)	S145P	probably damaging	Het
Vmn2r11	A	T	5: 109,202,850 (GRCm39)	F76I	probably benign	Het
Xpot	A	T	10: 121,432,149 (GRCm39)	C916*	probably null	Het
Zfp236	C	T	18: 82,637,042 (GRCm39)	E1415K	possibly damaging	Het

Other mutations in Or5b101

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00914:Or5b101	APN	19	13,004,955 (GRCm39)	missense	probably damaging	0.98
IGL01137:Or5b101	APN	19	13,005,394 (GRCm39)	missense	possibly damaging	0.52
IGL01894:Or5b101	APN	19	13,005,649 (GRCm39)	missense	probably damaging	1.00
IGL02579:Or5b101	APN	19	13,004,892 (GRCm39)	missense	probably damaging	0.99
PIT4418001:Or5b101	UTSW	19	13,005,095 (GRCm39)	nonsense	probably null
R0099:Or5b101	UTSW	19	13,005,165 (GRCm39)	missense	probably damaging	1.00
R0211:Or5b101	UTSW	19	13,005,646 (GRCm39)	missense	possibly damaging	0.95
R0211:Or5b101	UTSW	19	13,005,646 (GRCm39)	missense	possibly damaging	0.95
R0395:Or5b101	UTSW	19	13,005,663 (GRCm39)	missense	probably damaging	1.00
R0453:Or5b101	UTSW	19	13,005,295 (GRCm39)	missense	probably damaging	0.97
R0847:Or5b101	UTSW	19	13,005,095 (GRCm39)	nonsense	probably null
R1227:Or5b101	UTSW	19	13,005,217 (GRCm39)	missense	probably benign
R1823:Or5b101	UTSW	19	13,005,181 (GRCm39)	missense	probably benign	0.06
R2509:Or5b101	UTSW	19	13,005,058 (GRCm39)	missense	probably damaging	1.00
R2899:Or5b101	UTSW	19	13,005,058 (GRCm39)	missense	probably damaging	1.00
R2964:Or5b101	UTSW	19	13,005,412 (GRCm39)	missense	probably benign	0.38
R2965:Or5b101	UTSW	19	13,005,412 (GRCm39)	missense	probably benign	0.38
R3157:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R3158:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R3409:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R3410:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R3411:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R3425:Or5b101	UTSW	19	13,005,411 (GRCm39)	missense	probably benign	0.03
R4018:Or5b101	UTSW	19	13,005,189 (GRCm39)	missense	probably benign
R4668:Or5b101	UTSW	19	13,005,445 (GRCm39)	missense	probably benign	0.00
R4805:Or5b101	UTSW	19	13,005,661 (GRCm39)	missense	probably benign	0.04
R5180:Or5b101	UTSW	19	13,004,776 (GRCm39)	missense	probably benign	0.01
R5391:Or5b101	UTSW	19	13,005,150 (GRCm39)	missense	probably damaging	1.00
R5557:Or5b101	UTSW	19	13,005,004 (GRCm39)	missense	probably benign	0.01
R5740:Or5b101	UTSW	19	13,004,926 (GRCm39)	missense	probably benign	0.24
R7472:Or5b101	UTSW	19	13,005,439 (GRCm39)	missense	probably benign
R7832:Or5b101	UTSW	19	13,005,360 (GRCm39)	missense	probably benign	0.02
R7970:Or5b101	UTSW	19	13,005,058 (GRCm39)	missense	probably damaging	1.00
R8044:Or5b101	UTSW	19	13,004,829 (GRCm39)	missense	probably damaging	1.00
R8229:Or5b101	UTSW	19	13,005,561 (GRCm39)	missense	possibly damaging	0.55
R8474:Or5b101	UTSW	19	13,005,357 (GRCm39)	missense	probably damaging	1.00
R8951:Or5b101	UTSW	19	13,004,827 (GRCm39)	missense
R8957:Or5b101	UTSW	19	13,004,881 (GRCm39)	missense	probably benign	0.03
R9059:Or5b101	UTSW	19	13,005,277 (GRCm39)	missense	probably damaging	1.00
R9131:Or5b101	UTSW	19	13,005,360 (GRCm39)	missense	probably benign	0.02
R9266:Or5b101	UTSW	19	13,005,648 (GRCm39)	missense	probably damaging	1.00
R9269:Or5b101	UTSW	19	13,004,994 (GRCm39)	missense	probably benign
Z1088:Or5b101	UTSW	19	13,005,391 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GAGCTAGGCTGCAAGTACATG -3'
(R):5'- TGTAAGCCCCTACATTATGCTACC -3'

Sequencing Primer
(F):5'- CTAGGCTGCAAGTACATGAATATG -3'
(R):5'- CCCTACATTATGCTACCACCATG -3'

Posted On

2021-11-19