Mutagenetix > Incidental Mutation

Incidental Mutation 'R9047:Zfp81'

688151

Institutional Source

Beutler Lab

Gene Symbol

Zfp81

Ensembl Gene

ENSMUSG00000003929

Gene Name

zinc finger protein 81

Synonyms

KRAB13, Zfp78, Hszfp36, C330034P10Rik, D330034E10Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.299) question?

Stock #

R9047 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

33329342-33358878 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 33334413 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 476 (C476R)

Ref Sequence

ENSEMBL: ENSMUSP00000050728 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054072]

AlphaFold

B8JJZ7

Predicted Effect

probably damaging
Transcript: ENSMUST00000054072
AA Change: C476R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000050728
Gene: ENSMUSG00000003929
AA Change: C476R

Domain	Start	End	E-Value	Type
KRAB	4	55	1.15e-14	SMART
ZnF_C2H2	222	244	1.04e-3	SMART
ZnF_C2H2	250	272	1.53e-1	SMART
ZnF_C2H2	278	300	1.33e-1	SMART
ZnF_C2H2	306	328	5.21e-4	SMART
ZnF_C2H2	334	356	2.2e-2	SMART
ZnF_C2H2	362	384	1.58e-3	SMART
ZnF_C2H2	390	412	1.64e-1	SMART
ZnF_C2H2	418	440	7.67e-2	SMART
ZnF_C2H2	446	468	1.89e-1	SMART
ZnF_C2H2	474	496	2.86e-1	SMART
ZnF_C2H2	502	524	1.08e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.9%
20x: 99.7%

Validation Efficiency

100% (76/76)

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410004B18Rik	T	A	3: 145,939,093	S156T	probably damaging	Het
4930522L14Rik	A	G	5: 109,737,554	L146P		Het
Adra1a	T	A	14: 66,638,185	M203K	probably damaging	Het
Adtrp	G	A	13: 41,816,160	T89I	possibly damaging	Het
Akt3	G	T	1: 177,059,389	T298K	probably damaging	Het
Aldh4a1	G	T	4: 139,623,200		probably benign	Het
Aplf	T	C	6: 87,663,797	T77A	possibly damaging	Het
Arid4b	T	A	13: 14,181,230	W618R	probably damaging	Het
Asxl3	C	A	18: 22,452,408	P130Q	probably damaging	Het
Asxl3	A	G	18: 22,452,414	K132R	probably damaging	Het
Atp11a	T	C	8: 12,828,483	M353T	probably damaging	Het
Atp11b	A	G	3: 35,806,889	D375G	probably damaging	Het
BC052040	A	G	2: 115,777,023	T286A	probably benign	Het
Cage1	C	T	13: 38,017,362	A656T	possibly damaging	Het
Casz1	C	T	4: 148,939,040	P801S	probably damaging	Het
Ccdc130	C	T	8: 84,263,898	R35Q	probably damaging	Het
Ccdc144b	A	G	3: 36,032,884	I187T	probably benign	Het
Cdc14b	A	T	13: 64,220,944		probably benign	Het
Cox6b2	A	G	7: 4,752,087	F63L	probably benign	Het
Cramp1l	A	C	17: 24,979,629	V773G	possibly damaging	Het
Cul7	A	G	17: 46,654,522	E542G	probably benign	Het
Cyp2c55	T	C	19: 39,031,346	Y243H	possibly damaging	Het
Dnah9	C	A	11: 66,072,099	D1797Y	possibly damaging	Het
Dpep2	C	A	8: 105,989,312	A298S		Het
Dvl3	G	A	16: 20,524,076		probably null	Het
Enpp3	T	C	10: 24,798,274	D376G	possibly damaging	Het
Ffar4	T	C	19: 38,113,784	I289T	possibly damaging	Het
Gm3417	A	G	17: 14,967,680	Y111H	probably damaging	Het
Gm5565	T	C	5: 146,158,039	Y299C	probably damaging	Het
Gm8765	C	T	13: 50,702,092	R589*	probably null	Het
Gpat3	A	T	5: 100,846,922	M39L	probably benign	Het
Gpr153	T	C	4: 152,280,207	L240P	probably damaging	Het
Gpr182	T	C	10: 127,750,648	I145V	probably benign	Het
H2-Q4	A	T	17: 35,379,993	T80S	possibly damaging	Het
Hnf1a	T	A	5: 114,950,823	T545S	probably benign	Het
Kif5b	A	C	18: 6,208,261	F946V	probably benign	Het
Krtap16-1	C	T	11: 99,986,341	C79Y	probably damaging	Het
Lama2	A	G	10: 27,006,701	V2622A	possibly damaging	Het
Lrrc37a	T	A	11: 103,500,549	Q1350L	probably damaging	Het
Map2k2	G	A	10: 81,119,664	V294I	probably benign	Het
Mtus1	G	A	8: 41,083,723	H319Y	possibly damaging	Het
Ncdn	T	C	4: 126,750,828	D67G	possibly damaging	Het
Ngef	G	A	1: 87,503,288	P269L	probably damaging	Het
Nlrp9b	A	G	7: 20,023,476	I213V	possibly damaging	Het
Nyap2	G	A	1: 81,298,088	R649H	possibly damaging	Het
Olfr1165-ps	T	G	2: 88,101,072	D305A	unknown	Het
Olfr1200	C	A	2: 88,767,955	R120L	probably damaging	Het
Olfr304	A	G	7: 86,386,040	S207P	probably benign	Het
Pi4kb	T	A	3: 94,993,117	L354Q	probably damaging	Het
Plekhh2	T	C	17: 84,590,762	S944P	probably damaging	Het
Podn	C	T	4: 108,021,546	V375M	probably damaging	Het
Pramef8	C	A	4: 143,419,103	Q381K	possibly damaging	Het
Psmb2	T	A	4: 126,706,102	H110Q	probably benign	Het
Rasip1	A	G	7: 45,632,642	E523G	possibly damaging	Het
Ripor1	T	G	8: 105,616,151	C219G	probably damaging	Het
Rps6ka2	G	C	17: 7,300,279	V714L	probably damaging	Het
Sass6	G	T	3: 116,613,998	L254F	probably damaging	Het
Scarf2	G	A	16: 17,806,406	G525E	probably damaging	Het
Sh3d21	T	A	4: 126,152,338		probably benign	Het
Sh3tc1	C	A	5: 35,706,483	A787S	probably benign	Het
Skint5	T	A	4: 113,655,722	N871I	unknown	Het
Slc48a1	T	G	15: 97,789,952	D62E	probably damaging	Het
Slc6a21	G	A	7: 45,286,974	M157I		Het
Slurp2	T	C	15: 74,743,112	D60G	probably benign	Het
Sparcl1	G	T	5: 104,093,113	N148K	possibly damaging	Het
Spsb2	T	A	6: 124,810,013	N236K	probably benign	Het
Sptb	G	T	12: 76,632,534		probably benign	Het
Ssc4d	A	T	5: 135,961,176	C41S	probably damaging	Het
Tecta	A	T	9: 42,375,079	D760E	probably benign	Het
Tesmin	G	T	19: 3,389,431		probably benign	Het
Tiam1	A	C	16: 89,804,888		probably benign	Het
Tldc1	T	C	8: 119,762,311	N411S	probably benign	Het
Tnrc6a	T	C	7: 123,179,723	L1219S	probably damaging	Het
Tram1	T	G	1: 13,569,606	I306L	probably benign	Het
Trank1	A	C	9: 111,362,432	D503A	probably damaging	Het
Ttc21b	T	C	2: 66,201,252	H1044R		Het
Ubac2	T	C	14: 121,908,214	F95L	probably benign	Het
Vmn2r91	A	T	17: 18,106,034	M194L	probably benign	Het
Zdhhc1	G	T	8: 105,478,901	H90Q	probably damaging	Het
Zfp169	C	T	13: 48,498,816	V42I	probably damaging	Het

Other mutations in Zfp81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
feuer	UTSW	17	33334333	nonsense	probably null
R0143:Zfp81	UTSW	17	33335121	missense	possibly damaging	0.85
R0220:Zfp81	UTSW	17	33336724	missense	possibly damaging	0.93
R0520:Zfp81	UTSW	17	33334377	missense	probably damaging	0.97
R0611:Zfp81	UTSW	17	33334619	missense	probably benign	0.02
R1171:Zfp81	UTSW	17	33335280	missense	probably benign
R1613:Zfp81	UTSW	17	33334783	missense	probably damaging	0.98
R1779:Zfp81	UTSW	17	33335106	missense	probably benign	0.06
R1970:Zfp81	UTSW	17	33335501	missense	probably benign
R2063:Zfp81	UTSW	17	33335304	missense	probably benign	0.03
R2314:Zfp81	UTSW	17	33334623	missense	probably damaging	0.99
R2898:Zfp81	UTSW	17	33334300	nonsense	probably null
R3115:Zfp81	UTSW	17	33334563	missense	possibly damaging	0.80
R4207:Zfp81	UTSW	17	33334916	missense	probably damaging	1.00
R4497:Zfp81	UTSW	17	33334703	missense	possibly damaging	0.47
R4498:Zfp81	UTSW	17	33334703	missense	possibly damaging	0.47
R5756:Zfp81	UTSW	17	33334333	nonsense	probably null
R5964:Zfp81	UTSW	17	33336845	missense	probably damaging	0.99
R6671:Zfp81	UTSW	17	33335439	missense	probably benign	0.17
R7728:Zfp81	UTSW	17	33336817	missense	possibly damaging	0.95
R9561:Zfp81	UTSW	17	33334800	missense	probably benign	0.15
R9800:Zfp81	UTSW	17	33335437	nonsense	probably null
Z1176:Zfp81	UTSW	17	33334829	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TACATTGGTATAAGTTCTACCCACTAG -3'
(R):5'- ATGTAGTTATGGTCAGTCATATGAAAG -3'

Sequencing Primer
(F):5'- CCACTAGATTTCTTTTCACTGTGAG -3'
(R):5'- CCAGTGCCTGTTACATTCATGAGAG -3'

Posted On

2021-11-19