Incidental Mutation 'R9048:Siglecg'
ID 688174
Institutional Source Beutler Lab
Gene Symbol Siglecg
Ensembl Gene ENSMUSG00000030468
Gene Name sialic acid binding Ig-like lectin G
Synonyms mSiglec-G, A630096C01Rik
MMRRC Submission 068874-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.077) question?
Stock # R9048 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 43408204-43418358 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 43408579 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Leucine to Proline at position 7 (L7P)
Ref Sequence ENSEMBL: ENSMUSP00000005592 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000005592]
AlphaFold Q80ZE3
Predicted Effect probably benign
Transcript: ENSMUST00000005592
AA Change: L7P

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)
SMART Domains Protein: ENSMUSP00000005592
Gene: ENSMUSG00000030468
AA Change: L7P

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
IG 27 139 5.21e-2 SMART
IG_like 148 232 8.97e0 SMART
IGc2 262 325 3.38e-10 SMART
IGc2 366 427 8.26e-5 SMART
low complexity region 473 480 N/A INTRINSIC
transmembrane domain 545 564 N/A INTRINSIC
Meta Mutation Damage Score 0.6154 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.5%
Validation Efficiency 100% (53/53)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]
PHENOTYPE: Mice homozygous for a null allele exhibit increased B-1 cell numbers, increased IgM levels and IgM-producing plasma cells, and produce more IgM autoantibodies. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 53 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4932438A13Rik T C 3: 37,011,777 S67P Het
Als2 C T 1: 59,186,511 V947M possibly damaging Het
Atp5s C T 12: 69,740,978 R63C probably damaging Het
Bmp6 C T 13: 38,498,802 T453I probably damaging Het
Ccdc141 T C 2: 77,023,528 K1092R probably damaging Het
Ces1f T C 8: 93,263,067 D407G probably benign Het
Cfap46 G T 7: 139,627,343 H1887N unknown Het
Cldn10 G A 14: 118,788,244 V30M probably damaging Het
Col4a1 T A 8: 11,231,944 probably benign Het
Enpp7 A G 11: 118,990,629 H200R probably damaging Het
Epb41l3 C T 17: 69,210,223 A82V probably benign Het
Exph5 A G 9: 53,373,635 H672R possibly damaging Het
Fam189b T C 3: 89,185,741 Y269H probably damaging Het
Fam49a G A 12: 12,362,285 A183T probably damaging Het
Glce A T 9: 62,060,131 Y579* probably null Het
Gm4737 A T 16: 46,154,195 V273D probably damaging Het
Gm9507 G A 10: 77,811,411 P145S unknown Het
Igf2 A T 7: 142,654,022 F113I probably benign Het
Il1a T C 2: 129,306,521 S90G probably benign Het
Kalrn A G 16: 34,034,484 I2002T possibly damaging Het
Kng2 G A 16: 22,987,772 T559I probably benign Het
Leng8 A G 7: 4,143,932 probably benign Het
Lpcat2 G T 8: 92,909,250 A427S probably benign Het
Mapk14 A G 17: 28,728,384 T175A probably benign Het
Mei1 C A 15: 82,084,835 S329* probably null Het
Mfsd2a A G 4: 122,960,975 I20T probably benign Het
Mtcl1 T C 17: 66,371,336 N665S probably benign Het
Mup5 T A 4: 61,832,375 Q177L probably benign Het
Nkx6-2 A G 7: 139,581,960 F167S probably damaging Het
Olfr1386 T A 11: 49,471,053 W301R probably benign Het
Olfr1395 G A 11: 49,149,003 V249I probably damaging Het
Olfr156 T C 4: 43,820,584 Y259C probably damaging Het
Olfr16 T C 1: 172,957,362 V189A probably benign Het
Olfr186 G A 16: 59,027,235 T224I probably benign Het
Olfr543 A T 7: 102,477,477 I131N probably damaging Het
Phyh A T 2: 4,927,438 M112L probably benign Het
Pip5k1c C A 10: 81,316,876 probably benign Het
Pkn1 G A 8: 83,698,034 R11C possibly damaging Het
Podn G C 4: 108,021,399 R424G probably damaging Het
Ptdss2 A G 7: 141,152,884 D222G probably damaging Het
Ryk A T 9: 102,897,269 M401L probably benign Het
Sec61g A G 11: 16,508,129 V22A probably benign Het
Sept8 A G 11: 53,536,703 N250D probably damaging Het
Sin3a A G 9: 57,125,336 D1188G probably damaging Het
Sipa1l2 G A 8: 125,447,726 T1271M possibly damaging Het
Stard9 T A 2: 120,677,934 L241H probably damaging Het
Stx8 A G 11: 68,011,385 T132A probably damaging Het
Stxbp2 A G 8: 3,637,218 I403V Het
Stxbp3 A T 3: 108,816,388 M177K probably benign Het
Supt16 T A 14: 52,181,056 Q248L probably damaging Het
Tmem8 T C 17: 26,121,541 V633A probably damaging Het
Vmn2r91 A T 17: 18,135,860 L596F probably benign Het
Zkscan3 T C 13: 21,396,516 M1V probably null Het
Other mutations in Siglecg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00528:Siglecg APN 7 43409057 missense possibly damaging 0.64
IGL00556:Siglecg APN 7 43411795 missense probably benign 0.02
IGL01806:Siglecg APN 7 43411464 splice site probably null
IGL01947:Siglecg APN 7 43408763 missense probably benign 0.43
IGL02257:Siglecg APN 7 43411904 missense probably benign 0.00
IGL02410:Siglecg APN 7 43408829 missense probably damaging 0.99
IGL02454:Siglecg APN 7 43408895 missense probably benign 0.00
Chamonix UTSW 7 43409422 missense possibly damaging 0.91
Dollywood UTSW 7 43411099 missense probably damaging 1.00
glowworm UTSW 7 43408579 missense probably benign 0.04
Montblanc UTSW 7 43411386 intron probably benign
Shenandoah UTSW 7 43408802 missense probably damaging 0.99
shenandoah2 UTSW 7 43412017 missense possibly damaging 0.82
Sherando UTSW 7 43409057 missense possibly damaging 0.64
Smokies UTSW 7 43409279 missense probably benign 0.02
IGL02988:Siglecg UTSW 7 43418052 missense probably damaging 1.00
R0134:Siglecg UTSW 7 43411171 missense probably damaging 1.00
R0225:Siglecg UTSW 7 43411171 missense probably damaging 1.00
R0480:Siglecg UTSW 7 43411126 missense probably benign 0.42
R1538:Siglecg UTSW 7 43417889 missense possibly damaging 0.53
R1681:Siglecg UTSW 7 43408941 missense probably benign 0.17
R2358:Siglecg UTSW 7 43409422 missense possibly damaging 0.91
R4428:Siglecg UTSW 7 43417926 missense possibly damaging 0.84
R4429:Siglecg UTSW 7 43417926 missense possibly damaging 0.84
R4736:Siglecg UTSW 7 43417908 missense probably benign 0.03
R4754:Siglecg UTSW 7 43411871 intron probably benign
R5017:Siglecg UTSW 7 43411386 intron probably benign
R5713:Siglecg UTSW 7 43408802 missense probably damaging 0.99
R5777:Siglecg UTSW 7 43409413 missense possibly damaging 0.80
R5892:Siglecg UTSW 7 43412204 intron probably benign
R6153:Siglecg UTSW 7 43412017 missense possibly damaging 0.82
R6154:Siglecg UTSW 7 43412017 missense possibly damaging 0.82
R6331:Siglecg UTSW 7 43408754 missense possibly damaging 0.83
R6562:Siglecg UTSW 7 43409057 missense possibly damaging 0.64
R6749:Siglecg UTSW 7 43408979 missense probably benign 0.00
R7066:Siglecg UTSW 7 43411742 missense probably benign 0.40
R7884:Siglecg UTSW 7 43409279 missense probably benign 0.02
R8275:Siglecg UTSW 7 43412468 missense probably benign
R8554:Siglecg UTSW 7 43408896 missense probably benign 0.01
R8846:Siglecg UTSW 7 43412518 missense probably benign 0.02
R8873:Siglecg UTSW 7 43418024 missense probably benign 0.00
R8887:Siglecg UTSW 7 43408584 missense probably benign 0.18
R9012:Siglecg UTSW 7 43411099 missense probably damaging 1.00
R9032:Siglecg UTSW 7 43411625 missense probably benign 0.24
R9085:Siglecg UTSW 7 43411625 missense probably benign 0.24
R9313:Siglecg UTSW 7 43412432 missense probably benign 0.03
R9320:Siglecg UTSW 7 43409429 missense probably benign 0.33
R9745:Siglecg UTSW 7 43418052 missense probably damaging 0.98
RF006:Siglecg UTSW 7 43408864 nonsense probably null
Z1177:Siglecg UTSW 7 43412022 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GATGCAATGGGCTCGAAACTTG -3'
(R):5'- AAGATGCACAGACCCTCCTG -3'

Sequencing Primer
(F):5'- GCAGTTTGTCTTGCACCGGTAC -3'
(R):5'- GTAGGAAGTAGCTCTCCATCTGAC -3'
Posted On 2021-11-19