Mutagenetix > Incidental Mutation

Incidental Mutation 'R9055:Flnb'

688646

Institutional Source

Beutler Lab

Gene Symbol

Flnb

Ensembl Gene

ENSMUSG00000025278

Gene Name

filamin, beta

Synonyms

MMRRC Submission

068881-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9055 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

7817957-7951588 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 7908553 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 1310 (D1310E)

Ref Sequence

ENSEMBL: ENSMUSP00000052020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052678]

AlphaFold

Q80X90

Predicted Effect

probably benign
Transcript: ENSMUST00000052678
AA Change: D1310E

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000052020
Gene: ENSMUSG00000025278
AA Change: D1310E

Domain	Start	End	E-Value	Type
CH	18	120	2.38e-26	SMART
CH	141	237	1.83e-18	SMART
IG_FLMN	253	350	1.17e-33	SMART
IG_FLMN	353	449	2.08e-34	SMART
IG_FLMN	451	546	3.42e-35	SMART
IG_FLMN	548	639	6.06e-27	SMART
IG_FLMN	644	739	1.94e-34	SMART
IG_FLMN	741	842	4.25e-31	SMART
IG_FLMN	844	941	5.16e-30	SMART
IG_FLMN	943	1037	5.12e-25	SMART
IG_FLMN	1039	1130	5.31e-41	SMART
IG_FLMN	1132	1225	1.4e-31	SMART
IG_FLMN	1227	1325	1.42e-32	SMART
IG_FLMN	1327	1418	5.19e-35	SMART
IG_FLMN	1420	1514	2.48e-41	SMART
IG_FLMN	1516	1611	3.15e-34	SMART
IG_FLMN	1613	1707	4.99e-37	SMART
IG_FLMN	1730	1819	4.44e-11	SMART
IG_FLMN	1820	1911	5.82e-38	SMART
IG_FLMN	1914	1997	5.68e-9	SMART
IG_FLMN	2001	2092	4.45e-34	SMART
IG_FLMN	2101	2188	1.24e-9	SMART
IG_FLMN	2192	2283	4.48e-39	SMART
IG_FLMN	2286	2378	2.94e-25	SMART
IG_FLMN	2383	2474	5.66e-27	SMART
IG_FLMN	2511	2602	1.63e-27	SMART

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mutations in this gene cause skeletal defects including runting, premature mineralization, and bone fusion. Nullizygous mice show a delay and reduction in long bone growth. Truncation mutations cause early fusion of spinal vertebrae due to enhanced chondrocyte hypertrophy and early differentiation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	T	A	19: 57,041,966 (GRCm38)	T753S	probably benign	Het
Acy3	A	G	19: 3,988,178 (GRCm38)	E157G	probably benign	Het
Adamts20	A	G	15: 94,283,986 (GRCm38)	Y1609H	probably damaging	Het
Ahnak2	C	T	12: 112,774,585 (GRCm38)	A1018T	possibly damaging	Het
Ahsg	T	A	16: 22,892,319 (GRCm38)	W69R	possibly damaging	Het
Alox12	T	G	11: 70,253,077 (GRCm38)	D159A	probably damaging	Het
Alox12b	C	G	11: 69,164,058 (GRCm38)	P296A	possibly damaging	Het
Ankrd17	T	C	5: 90,232,309 (GRCm38)	I2507V	probably benign	Het
Apeh	T	C	9: 108,085,846 (GRCm38)	M670V	possibly damaging	Het
Aqp12	C	T	1: 93,006,905 (GRCm38)	A168V	probably benign	Het
Ceacam1	A	T	7: 25,471,874 (GRCm38)	D388E	probably damaging	Het
Clp1	A	T	2: 84,723,922 (GRCm38)	I301N	probably damaging	Het
Cntn3	A	G	6: 102,267,437 (GRCm38)	F384L	probably benign	Het
Dnpep	A	T	1: 75,315,161 (GRCm38)	M238K	possibly damaging	Het
Dync2h1	A	T	9: 6,996,641 (GRCm38)		probably benign	Het
Edc3	T	A	9: 57,716,077 (GRCm38)	N98K	probably benign	Het
Egr3	T	A	14: 70,078,900 (GRCm38)	N15K	probably damaging	Het
Fbxo25	T	A	8: 13,915,023 (GRCm38)	C25S	possibly damaging	Het
Fgd4	T	A	16: 16,422,630 (GRCm38)	D745V	possibly damaging	Het
Ggnbp2	A	G	11: 84,841,622 (GRCm38)	F215S	probably damaging	Het
Gm1979	C	T	5: 26,002,034 (GRCm38)	R107Q	probably benign	Het
Gm4847	T	A	1: 166,640,108 (GRCm38)	H173L	probably damaging	Het
Gmeb1	A	T	4: 132,237,114 (GRCm38)	L146H	probably damaging	Het
Gpr88	A	G	3: 116,252,651 (GRCm38)	S4P	unknown	Het
Gucy1a1	A	G	3: 82,109,126 (GRCm38)	L185P	possibly damaging	Het
Hsd3b6	G	T	3: 98,806,668 (GRCm38)	T105N	probably damaging	Het
Iqca1	T	C	1: 90,070,613 (GRCm38)	Y546C	probably benign	Het
Irf1	T	A	11: 53,776,370 (GRCm38)	I305N	probably benign	Het
Itgb3	G	A	11: 104,665,625 (GRCm38)	W764*	probably null	Het
Klra8	G	T	6: 130,119,054 (GRCm38)	R192S	probably benign	Het
Krt79	T	A	15: 101,931,487 (GRCm38)	I358F	probably damaging	Het
Mettl4	T	C	17: 94,740,415 (GRCm38)	D266G	possibly damaging	Het
Mfsd8	G	A	3: 40,832,058 (GRCm38)	A219V	probably benign	Het
Mgam	G	A	6: 40,714,729 (GRCm38)		probably benign	Het
Nek9	A	T	12: 85,301,842 (GRCm38)	C973S	probably damaging	Het
Or2k2	G	T	4: 58,785,374 (GRCm38)	A116E	possibly damaging	Het
Or4f15	A	T	2: 111,983,704 (GRCm38)	Y123*	probably null	Het
Or8c13	T	C	9: 38,180,484 (GRCm38)	Y113C	probably damaging	Het
Ostc	T	A	3: 130,709,371 (GRCm38)	E2V	probably damaging	Het
Ostc	C	A	3: 130,709,372 (GRCm38)	E2*	probably null	Het
Pcdhb8	T	A	18: 37,357,532 (GRCm38)	C754*	probably null	Het
Polr1a	A	G	6: 71,915,069 (GRCm38)	T111A	possibly damaging	Het
Prep	C	T	10: 45,115,195 (GRCm38)	T319M	probably benign	Het
Reg3b	A	C	6: 78,372,903 (GRCm38)	D142A	possibly damaging	Het
Retsat	A	G	6: 72,606,953 (GRCm38)	D537G	probably benign	Het
Rnf2	G	A	1: 151,476,279 (GRCm38)	L109F	probably damaging	Het
Rskr	A	G	11: 78,293,547 (GRCm38)	D240G	probably damaging	Het
Runx3	G	T	4: 135,175,345 (GRCm38)	R262L	probably damaging	Het
Rwdd3	G	T	3: 121,159,222 (GRCm38)	T112K	probably benign	Het
Scfd2	T	C	5: 74,531,270 (GRCm38)	H117R	possibly damaging	Het
Scn10a	C	T	9: 119,622,892 (GRCm38)	V1321M	probably damaging	Het
Sec31a	T	C	5: 100,386,181 (GRCm38)	E119G	possibly damaging	Het
Skint6	C	A	4: 113,238,150 (GRCm38)	G104V	probably damaging	Het
Slc18a1	T	A	8: 69,068,171 (GRCm38)	I199F	possibly damaging	Het
St18	C	T	1: 6,802,982 (GRCm38)	R314*	probably null	Het
Sucla2	T	G	14: 73,581,628 (GRCm38)		probably benign	Het
Sulf1	A	T	1: 12,807,963 (GRCm38)	H225L	probably damaging	Het
Tas2r140	A	G	6: 133,055,417 (GRCm38)	V126A	possibly damaging	Het
Tcp10b	T	C	17: 13,062,941 (GRCm38)	I66T	probably damaging	Het
Tmem45a2	T	C	16: 57,040,752 (GRCm38)	I237M	probably benign	Het
Traf3ip1	C	T	1: 91,501,011 (GRCm38)	R167*	probably null	Het
Trav17	T	G	14: 53,806,863 (GRCm38)	V30G	probably damaging	Het
Vmn1r54	A	G	6: 90,269,118 (GRCm38)	N5D	probably benign	Het
Vmn2r81	A	G	10: 79,274,607 (GRCm38)	H518R	probably benign	Het
Washc3	A	G	10: 88,216,054 (GRCm38)	T102A	probably benign	Het
Xaf1	A	G	11: 72,303,440 (GRCm38)	H49R	probably damaging	Het
Zfp1001	G	A	2: 150,323,833 (GRCm38)		probably benign	Het
Zfp276	T	C	8: 123,258,370 (GRCm38)	F356L	probably damaging	Het
Zfp398	G	T	6: 47,866,385 (GRCm38)	R457L	possibly damaging	Het
Zfp512	G	T	5: 31,480,189 (GRCm38)	E541*	probably null	Het

Other mutations in Flnb

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01017:Flnb	APN	14	7,917,390 (GRCm38)	splice site	probably benign
IGL01063:Flnb	APN	14	7,926,518 (GRCm38)	splice site	probably benign
IGL01135:Flnb	APN	14	7,909,736 (GRCm38)	missense	probably benign
IGL01139:Flnb	APN	14	7,945,989 (GRCm38)	missense	probably damaging	1.00
IGL01364:Flnb	APN	14	7,934,562 (GRCm38)	critical splice acceptor site	probably null
IGL01417:Flnb	APN	14	7,905,513 (GRCm38)	missense	probably damaging	0.99
IGL01505:Flnb	APN	14	7,902,003 (GRCm38)	critical splice donor site	probably null
IGL01560:Flnb	APN	14	7,893,829 (GRCm38)	missense	probably benign	0.07
IGL01621:Flnb	APN	14	7,950,470 (GRCm38)	missense	probably damaging	1.00
IGL01656:Flnb	APN	14	7,902,010 (GRCm38)	splice site	probably benign
IGL01889:Flnb	APN	14	7,935,967 (GRCm38)	missense	possibly damaging	0.85
IGL01987:Flnb	APN	14	7,922,748 (GRCm38)	critical splice donor site	probably null
IGL02322:Flnb	APN	14	7,894,676 (GRCm38)	missense	probably damaging	1.00
IGL02496:Flnb	APN	14	7,930,919 (GRCm38)	splice site	probably benign
IGL02752:Flnb	APN	14	7,917,338 (GRCm38)	missense	probably benign
IGL03001:Flnb	APN	14	7,934,680 (GRCm38)	missense	probably damaging	0.99
IGL03076:Flnb	APN	14	7,901,988 (GRCm38)	missense	probably benign	0.01
IGL03085:Flnb	APN	14	7,882,211 (GRCm38)	missense	probably benign
IGL03170:Flnb	APN	14	7,818,261 (GRCm38)	missense	possibly damaging	0.90
IGL03373:Flnb	APN	14	7,890,867 (GRCm38)	critical splice donor site	probably null
Boomerang	UTSW	14	7,901,945 (GRCm38)	missense	probably damaging	1.00
Queensland	UTSW	14	7,927,352 (GRCm38)	missense	probably damaging	1.00
R3437_Flnb_252	UTSW	14	7,942,057 (GRCm38)	missense	probably damaging	0.97
R8441_Flnb_221	UTSW	14	7,896,488 (GRCm38)	missense	probably benign	0.15
Rhodelinda	UTSW	14	7,887,682 (GRCm38)	splice site	probably benign
saul	UTSW	14	7,889,183 (GRCm38)	missense	probably damaging	0.99
Xerxes	UTSW	14	7,867,551 (GRCm38)	missense	probably damaging	1.00
R0068:Flnb	UTSW	14	7,915,290 (GRCm38)	missense	possibly damaging	0.49
R0068:Flnb	UTSW	14	7,915,290 (GRCm38)	missense	possibly damaging	0.49
R0084:Flnb	UTSW	14	7,935,979 (GRCm38)	missense	probably benign
R0128:Flnb	UTSW	14	7,901,951 (GRCm38)	missense	probably damaging	0.99
R0130:Flnb	UTSW	14	7,901,951 (GRCm38)	missense	probably damaging	0.99
R0148:Flnb	UTSW	14	7,939,077 (GRCm38)	missense	probably benign	0.01
R0166:Flnb	UTSW	14	7,896,115 (GRCm38)	missense	probably damaging	1.00
R0376:Flnb	UTSW	14	7,946,014 (GRCm38)	critical splice donor site	probably null
R0547:Flnb	UTSW	14	7,912,943 (GRCm38)	splice site	probably null
R0612:Flnb	UTSW	14	7,887,682 (GRCm38)	splice site	probably benign
R0656:Flnb	UTSW	14	7,927,352 (GRCm38)	missense	probably damaging	1.00
R0691:Flnb	UTSW	14	7,890,810 (GRCm38)	missense	probably benign	0.16
R1241:Flnb	UTSW	14	7,896,503 (GRCm38)	missense	probably benign	0.06
R1572:Flnb	UTSW	14	7,883,908 (GRCm38)	missense	probably damaging	0.97
R1682:Flnb	UTSW	14	7,913,121 (GRCm38)	missense	probably benign	0.04
R1807:Flnb	UTSW	14	7,934,645 (GRCm38)	missense	probably benign	0.26
R1848:Flnb	UTSW	14	7,892,113 (GRCm38)	missense	probably damaging	1.00
R1959:Flnb	UTSW	14	7,884,735 (GRCm38)	nonsense	probably null
R2078:Flnb	UTSW	14	7,927,466 (GRCm38)	missense	probably damaging	1.00
R2132:Flnb	UTSW	14	7,873,376 (GRCm38)	missense	probably benign	0.04
R2209:Flnb	UTSW	14	7,905,507 (GRCm38)	nonsense	probably null
R2212:Flnb	UTSW	14	7,881,652 (GRCm38)	small deletion	probably benign
R2213:Flnb	UTSW	14	7,881,652 (GRCm38)	small deletion	probably benign
R2363:Flnb	UTSW	14	7,945,950 (GRCm38)	missense	possibly damaging	0.95
R2415:Flnb	UTSW	14	7,929,932 (GRCm38)	missense	probably benign	0.07
R2983:Flnb	UTSW	14	7,882,250 (GRCm38)	missense	probably damaging	1.00
R3001:Flnb	UTSW	14	7,907,162 (GRCm38)	missense	probably benign	0.22
R3002:Flnb	UTSW	14	7,907,162 (GRCm38)	missense	probably benign	0.22
R3436:Flnb	UTSW	14	7,942,057 (GRCm38)	missense	probably damaging	0.97
R3437:Flnb	UTSW	14	7,942,057 (GRCm38)	missense	probably damaging	0.97
R3778:Flnb	UTSW	14	7,915,353 (GRCm38)	missense	probably benign	0.06
R3783:Flnb	UTSW	14	7,889,236 (GRCm38)	missense	probably benign	0.04
R4162:Flnb	UTSW	14	7,915,374 (GRCm38)	missense	possibly damaging	0.81
R4163:Flnb	UTSW	14	7,915,374 (GRCm38)	missense	possibly damaging	0.81
R4164:Flnb	UTSW	14	7,915,374 (GRCm38)	missense	possibly damaging	0.81
R4356:Flnb	UTSW	14	7,922,700 (GRCm38)	missense	probably benign
R4369:Flnb	UTSW	14	7,942,216 (GRCm38)	missense	probably benign
R4783:Flnb	UTSW	14	7,905,701 (GRCm38)	missense	probably benign	0.12
R4785:Flnb	UTSW	14	7,905,701 (GRCm38)	missense	probably benign	0.12
R4790:Flnb	UTSW	14	7,905,661 (GRCm38)	missense	probably benign	0.34
R4828:Flnb	UTSW	14	7,919,238 (GRCm38)	missense	probably benign	0.13
R4882:Flnb	UTSW	14	7,929,936 (GRCm38)	missense	possibly damaging	0.56
R5002:Flnb	UTSW	14	7,945,882 (GRCm38)	missense	probably damaging	1.00
R5058:Flnb	UTSW	14	7,924,262 (GRCm38)	nonsense	probably null
R5184:Flnb	UTSW	14	7,901,945 (GRCm38)	missense	probably damaging	1.00
R5186:Flnb	UTSW	14	7,909,748 (GRCm38)	missense	probably damaging	1.00
R5395:Flnb	UTSW	14	7,883,881 (GRCm38)	missense	probably benign	0.02
R5421:Flnb	UTSW	14	7,926,494 (GRCm38)	missense	probably damaging	1.00
R5667:Flnb	UTSW	14	7,890,843 (GRCm38)	missense	probably benign	0.00
R5671:Flnb	UTSW	14	7,890,843 (GRCm38)	missense	probably benign	0.00
R5714:Flnb	UTSW	14	7,929,073 (GRCm38)	missense	probably damaging	1.00
R5860:Flnb	UTSW	14	7,931,135 (GRCm38)	missense	probably damaging	1.00
R5892:Flnb	UTSW	14	7,907,183 (GRCm38)	missense	probably damaging	1.00
R5924:Flnb	UTSW	14	7,890,765 (GRCm38)	missense	probably benign	0.00
R6131:Flnb	UTSW	14	7,894,635 (GRCm38)	missense	possibly damaging	0.79
R6244:Flnb	UTSW	14	7,892,092 (GRCm38)	missense	probably damaging	1.00
R6489:Flnb	UTSW	14	7,867,551 (GRCm38)	missense	probably damaging	1.00
R6582:Flnb	UTSW	14	7,892,275 (GRCm38)	critical splice donor site	probably null
R6586:Flnb	UTSW	14	7,929,138 (GRCm38)	missense	possibly damaging	0.93
R6611:Flnb	UTSW	14	7,915,318 (GRCm38)	missense	probably damaging	1.00
R6626:Flnb	UTSW	14	7,929,012 (GRCm38)	missense	probably damaging	1.00
R6700:Flnb	UTSW	14	7,892,189 (GRCm38)	missense	probably damaging	0.99
R6738:Flnb	UTSW	14	7,904,536 (GRCm38)	missense	probably benign	0.01
R6864:Flnb	UTSW	14	7,905,640 (GRCm38)	missense	possibly damaging	0.84
R6916:Flnb	UTSW	14	7,907,171 (GRCm38)	missense	probably damaging	0.99
R7117:Flnb	UTSW	14	7,894,214 (GRCm38)	missense	probably benign	0.02
R7164:Flnb	UTSW	14	7,915,944 (GRCm38)	splice site	probably null
R7328:Flnb	UTSW	14	7,894,660 (GRCm38)	nonsense	probably null
R7328:Flnb	UTSW	14	7,883,788 (GRCm38)	missense	possibly damaging	0.95
R7687:Flnb	UTSW	14	7,924,224 (GRCm38)	missense	probably damaging	1.00
R7716:Flnb	UTSW	14	7,917,274 (GRCm38)	missense	possibly damaging	0.64
R7763:Flnb	UTSW	14	7,926,478 (GRCm38)	missense	probably benign	0.00
R7821:Flnb	UTSW	14	7,939,113 (GRCm38)	missense	probably benign	0.00
R7921:Flnb	UTSW	14	7,933,800 (GRCm38)	missense	possibly damaging	0.57
R8008:Flnb	UTSW	14	7,892,155 (GRCm38)	missense	probably damaging	1.00
R8075:Flnb	UTSW	14	7,913,048 (GRCm38)	missense	probably benign	0.00
R8084:Flnb	UTSW	14	7,907,243 (GRCm38)	missense	probably benign	0.00
R8259:Flnb	UTSW	14	7,889,183 (GRCm38)	missense	probably damaging	0.99
R8441:Flnb	UTSW	14	7,896,488 (GRCm38)	missense	probably benign	0.15
R8493:Flnb	UTSW	14	7,869,822 (GRCm38)	missense	probably damaging	0.97
R8508:Flnb	UTSW	14	7,950,394 (GRCm38)	missense	probably damaging	0.98
R8531:Flnb	UTSW	14	7,929,939 (GRCm38)	missense	probably damaging	1.00
R8812:Flnb	UTSW	14	7,887,624 (GRCm38)	missense	probably benign	0.06
R8814:Flnb	UTSW	14	7,927,409 (GRCm38)	missense	probably damaging	1.00
R8825:Flnb	UTSW	14	7,887,566 (GRCm38)	missense	probably damaging	1.00
R8868:Flnb	UTSW	14	7,908,671 (GRCm38)	missense	probably benign	0.02
R8955:Flnb	UTSW	14	7,904,688 (GRCm38)	nonsense	probably null
R8955:Flnb	UTSW	14	7,892,874 (GRCm38)	missense	probably damaging	1.00
R8976:Flnb	UTSW	14	7,901,882 (GRCm38)	critical splice acceptor site	probably null
R9148:Flnb	UTSW	14	7,817,996 (GRCm38)	start gained	probably benign
R9179:Flnb	UTSW	14	7,887,541 (GRCm38)	nonsense	probably null
R9180:Flnb	UTSW	14	7,818,219 (GRCm38)	missense	probably damaging	1.00
R9189:Flnb	UTSW	14	7,892,976 (GRCm38)	missense	possibly damaging	0.90
R9286:Flnb	UTSW	14	7,873,414 (GRCm38)	missense	probably damaging	0.98
R9288:Flnb	UTSW	14	7,904,498 (GRCm38)	missense	probably benign	0.43
R9354:Flnb	UTSW	14	7,818,411 (GRCm38)	missense	probably benign	0.13
R9484:Flnb	UTSW	14	7,929,004 (GRCm38)	missense	probably benign	0.06
R9505:Flnb	UTSW	14	7,904,665 (GRCm38)	missense	probably benign
R9525:Flnb	UTSW	14	7,905,481 (GRCm38)	missense	probably damaging	1.00
R9621:Flnb	UTSW	14	7,926,421 (GRCm38)	missense	probably damaging	0.99
R9630:Flnb	UTSW	14	7,926,438 (GRCm38)	nonsense	probably null
R9739:Flnb	UTSW	14	7,935,954 (GRCm38)	nonsense	probably null
R9760:Flnb	UTSW	14	7,929,846 (GRCm38)	missense	probably damaging	0.98
X0066:Flnb	UTSW	14	7,908,636 (GRCm38)	missense	probably damaging	1.00
Z1088:Flnb	UTSW	14	7,905,871 (GRCm38)	missense	probably benign	0.04
Z1176:Flnb	UTSW	14	7,942,066 (GRCm38)	missense	probably benign	0.25

Predicted Primers

PCR Primer
(F):5'- AAATTGAAAGTACACTGGCCTTACC -3'
(R):5'- GGGGATCAATACCCTGAACC -3'

Sequencing Primer
(F):5'- CATTAGAGTCCTGGACCTGGAG -3'
(R):5'- TGAACCCAGGACCCTGC -3'

Posted On

2021-11-19