Mutagenetix > Incidental Mutation

Incidental Mutation 'R9055:Mettl4'

688655

Institutional Source

Beutler Lab

Gene Symbol

Mettl4

Ensembl Gene

ENSMUSG00000055660

Gene Name

methyltransferase like 4

Synonyms

HsT661, A730091E08Rik, 2410198H06Rik

MMRRC Submission

068881-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.288) question?

Stock #

R9055 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

94727080-94749892 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 94740415 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 266 (D266G)

Ref Sequence

ENSEMBL: ENSMUSP00000127142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171284]

AlphaFold

Q3U034

Predicted Effect

possibly damaging
Transcript: ENSMUST00000171284
AA Change: D266G

PolyPhen 2 Score 0.647 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000127142
Gene: ENSMUSG00000055660
AA Change: D266G

Domain	Start	End	E-Value	Type
Pfam:MT-A70	280	454	9.9e-43	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

97% (68/70)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	T	A	19: 57,041,966 (GRCm38)	T753S	probably benign	Het
Acy3	A	G	19: 3,988,178 (GRCm38)	E157G	probably benign	Het
Adamts20	A	G	15: 94,283,986 (GRCm38)	Y1609H	probably damaging	Het
Ahnak2	C	T	12: 112,774,585 (GRCm38)	A1018T	possibly damaging	Het
Ahsg	T	A	16: 22,892,319 (GRCm38)	W69R	possibly damaging	Het
Alox12	T	G	11: 70,253,077 (GRCm38)	D159A	probably damaging	Het
Alox12b	C	G	11: 69,164,058 (GRCm38)	P296A	possibly damaging	Het
Ankrd17	T	C	5: 90,232,309 (GRCm38)	I2507V	probably benign	Het
Apeh	T	C	9: 108,085,846 (GRCm38)	M670V	possibly damaging	Het
Aqp12	C	T	1: 93,006,905 (GRCm38)	A168V	probably benign	Het
BC030499	A	G	11: 78,293,547 (GRCm38)	D240G	probably damaging	Het
Ceacam1	A	T	7: 25,471,874 (GRCm38)	D388E	probably damaging	Het
Clp1	A	T	2: 84,723,922 (GRCm38)	I301N	probably damaging	Het
Cntn3	A	G	6: 102,267,437 (GRCm38)	F384L	probably benign	Het
Dnpep	A	T	1: 75,315,161 (GRCm38)	M238K	possibly damaging	Het
Dync2h1	A	T	9: 6,996,641 (GRCm38)		probably benign	Het
Edc3	T	A	9: 57,716,077 (GRCm38)	N98K	probably benign	Het
Egr3	T	A	14: 70,078,900 (GRCm38)	N15K	probably damaging	Het
Fbxo25	T	A	8: 13,915,023 (GRCm38)	C25S	possibly damaging	Het
Fgd4	T	A	16: 16,422,630 (GRCm38)	D745V	possibly damaging	Het
Flnb	T	A	14: 7,908,553 (GRCm38)	D1310E	probably benign	Het
Ggnbp2	A	G	11: 84,841,622 (GRCm38)	F215S	probably damaging	Het
Gm10130	G	A	2: 150,323,833 (GRCm38)		probably benign	Het
Gm1979	C	T	5: 26,002,034 (GRCm38)	R107Q	probably benign	Het
Gm4847	T	A	1: 166,640,108 (GRCm38)	H173L	probably damaging	Het
Gmeb1	A	T	4: 132,237,114 (GRCm38)	L146H	probably damaging	Het
Gpr88	A	G	3: 116,252,651 (GRCm38)	S4P	unknown	Het
Gucy1a1	A	G	3: 82,109,126 (GRCm38)	L185P	possibly damaging	Het
Hsd3b6	G	T	3: 98,806,668 (GRCm38)	T105N	probably damaging	Het
Iqca	T	C	1: 90,070,613 (GRCm38)	Y546C	probably benign	Het
Irf1	T	A	11: 53,776,370 (GRCm38)	I305N	probably benign	Het
Itgb3	G	A	11: 104,665,625 (GRCm38)	W764*	probably null	Het
Klra8	G	T	6: 130,119,054 (GRCm38)	R192S	probably benign	Het
Krt79	T	A	15: 101,931,487 (GRCm38)	I358F	probably damaging	Het
Mfsd8	G	A	3: 40,832,058 (GRCm38)	A219V	probably benign	Het
Mgam	G	A	6: 40,714,729 (GRCm38)		probably benign	Het
Nek9	A	T	12: 85,301,842 (GRCm38)	C973S	probably damaging	Het
Olfr1309	A	T	2: 111,983,704 (GRCm38)	Y123*	probably null	Het
Olfr267	G	T	4: 58,785,374 (GRCm38)	A116E	possibly damaging	Het
Olfr891	T	C	9: 38,180,484 (GRCm38)	Y113C	probably damaging	Het
Ostc	C	A	3: 130,709,372 (GRCm38)	E2*	probably null	Het
Ostc	T	A	3: 130,709,371 (GRCm38)	E2V	probably damaging	Het
Pcdhb8	T	A	18: 37,357,532 (GRCm38)	C754*	probably null	Het
Polr1a	A	G	6: 71,915,069 (GRCm38)	T111A	possibly damaging	Het
Prep	C	T	10: 45,115,195 (GRCm38)	T319M	probably benign	Het
Reg3b	A	C	6: 78,372,903 (GRCm38)	D142A	possibly damaging	Het
Retsat	A	G	6: 72,606,953 (GRCm38)	D537G	probably benign	Het
Rnf2	G	A	1: 151,476,279 (GRCm38)	L109F	probably damaging	Het
Runx3	G	T	4: 135,175,345 (GRCm38)	R262L	probably damaging	Het
Rwdd3	G	T	3: 121,159,222 (GRCm38)	T112K	probably benign	Het
Scfd2	T	C	5: 74,531,270 (GRCm38)	H117R	possibly damaging	Het
Scn10a	C	T	9: 119,622,892 (GRCm38)	V1321M	probably damaging	Het
Sec31a	T	C	5: 100,386,181 (GRCm38)	E119G	possibly damaging	Het
Skint6	C	A	4: 113,238,150 (GRCm38)	G104V	probably damaging	Het
Slc18a1	T	A	8: 69,068,171 (GRCm38)	I199F	possibly damaging	Het
St18	C	T	1: 6,802,982 (GRCm38)	R314*	probably null	Het
Sucla2	T	G	14: 73,581,628 (GRCm38)		probably benign	Het
Sulf1	A	T	1: 12,807,963 (GRCm38)	H225L	probably damaging	Het
Tas2r140	A	G	6: 133,055,417 (GRCm38)	V126A	possibly damaging	Het
Tcp10b	T	C	17: 13,062,941 (GRCm38)	I66T	probably damaging	Het
Tmem45a2	T	C	16: 57,040,752 (GRCm38)	I237M	probably benign	Het
Traf3ip1	C	T	1: 91,501,011 (GRCm38)	R167*	probably null	Het
Trav17	T	G	14: 53,806,863 (GRCm38)	V30G	probably damaging	Het
Vmn1r54	A	G	6: 90,269,118 (GRCm38)	N5D	probably benign	Het
Vmn2r81	A	G	10: 79,274,607 (GRCm38)	H518R	probably benign	Het
Washc3	A	G	10: 88,216,054 (GRCm38)	T102A	probably benign	Het
Xaf1	A	G	11: 72,303,440 (GRCm38)	H49R	probably damaging	Het
Zfp276	T	C	8: 123,258,370 (GRCm38)	F356L	probably damaging	Het
Zfp398	G	T	6: 47,866,385 (GRCm38)	R457L	possibly damaging	Het
Zfp512	G	T	5: 31,480,189 (GRCm38)	E541*	probably null	Het

Other mutations in Mettl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03376:Mettl4	APN	17	94,735,371 (GRCm38)	missense	probably damaging	1.00
R1938:Mettl4	UTSW	17	94,747,857 (GRCm38)	missense	possibly damaging	0.76
R2172:Mettl4	UTSW	17	94,733,163 (GRCm38)	missense	probably benign
R2370:Mettl4	UTSW	17	94,733,148 (GRCm38)	missense	probably damaging	1.00
R3621:Mettl4	UTSW	17	94,735,508 (GRCm38)	missense	probably damaging	1.00
R3913:Mettl4	UTSW	17	94,740,532 (GRCm38)	missense	probably benign	0.06
R4155:Mettl4	UTSW	17	94,740,575 (GRCm38)	missense	probably benign
R4536:Mettl4	UTSW	17	94,735,505 (GRCm38)	missense	possibly damaging	0.79
R4946:Mettl4	UTSW	17	94,740,532 (GRCm38)	missense	probably benign	0.06
R5263:Mettl4	UTSW	17	94,740,509 (GRCm38)	nonsense	probably null
R5397:Mettl4	UTSW	17	94,727,277 (GRCm38)	nonsense	probably null
R6242:Mettl4	UTSW	17	94,735,374 (GRCm38)	missense	probably damaging	1.00
R6508:Mettl4	UTSW	17	94,743,945 (GRCm38)	missense	probably damaging	0.98
R7069:Mettl4	UTSW	17	94,733,633 (GRCm38)	missense	probably damaging	0.98
R7941:Mettl4	UTSW	17	94,733,194 (GRCm38)	splice site	probably null
R8088:Mettl4	UTSW	17	94,735,367 (GRCm38)	missense	probably damaging	1.00
R8373:Mettl4	UTSW	17	94,733,649 (GRCm38)	missense	probably damaging	1.00
R8710:Mettl4	UTSW	17	94,733,644 (GRCm38)	missense	probably damaging	0.99
R9130:Mettl4	UTSW	17	94,735,485 (GRCm38)	missense	possibly damaging	0.61
R9335:Mettl4	UTSW	17	94,735,508 (GRCm38)	missense	probably damaging	1.00
R9697:Mettl4	UTSW	17	94,727,378 (GRCm38)	missense	probably damaging	0.98
Z1176:Mettl4	UTSW	17	94,733,563 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTAAGTCTGAACCTCTCAATTCTG -3'
(R):5'- GCCTTCCTTAAATGAAATGGAGCTTC -3'

Sequencing Primer
(F):5'- TGAGATGCCAAGGCTATCCTG -3'
(R):5'- CTTCAAACGCTACAGTTGATGGG -3'

Posted On

2021-11-19