Mutagenetix > Incidental Mutation

Incidental Mutation 'R9065:Msrb2'

689185

Institutional Source

Beutler Lab

Gene Symbol

Msrb2

Ensembl Gene

ENSMUSG00000023094

Gene Name

methionine sulfoxide reductase B2

Synonyms

2310050L06Rik, Msrb

MMRRC Submission

068890-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R9065 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19376251-19399787 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19383041 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 56 (F56I)

Ref Sequence

ENSEMBL: ENSMUSP00000023856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023856]

AlphaFold

Q78J03

PDB Structure

Structure-Functional Analysis of Mammalian MsrB2 protein [SOLUTION NMR]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000023856
AA Change: F56I

PolyPhen 2 Score 0.841 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000023856
Gene: ENSMUSG00000023094
AA Change: F56I

Domain	Start	End	E-Value	Type
low complexity region	2	22	N/A	INTRINSIC
Pfam:SelR	45	172	2.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	T	C	5: 113,323,084 (GRCm39)	N1472S	probably benign	Het
4930447C04Rik	C	A	12: 72,939,604 (GRCm39)	Q435H	possibly damaging	Het
Aass	G	T	6: 23,075,746 (GRCm39)	A797E	probably benign	Het
Ap3b1	T	A	13: 94,608,223 (GRCm39)	Y565N	probably damaging	Het
Arid2	A	G	15: 96,269,372 (GRCm39)	T1162A	probably benign	Het
Atp11b	T	C	3: 35,887,131 (GRCm39)		probably null	Het
Card11	G	A	5: 140,894,297 (GRCm39)	P111S	probably damaging	Het
Ccdc66	T	A	14: 27,213,850 (GRCm39)	E328D	probably damaging	Het
Cldn8	T	A	16: 88,359,902 (GRCm39)	M8L	probably benign	Het
Cnot9	T	A	1: 74,558,142 (GRCm39)	L96*	probably null	Het
Cntnap5c	T	A	17: 58,445,642 (GRCm39)	C561S	probably damaging	Het
Dnah5	A	G	15: 28,293,936 (GRCm39)	D1347G	probably benign	Het
Epb41	A	G	4: 131,682,888 (GRCm39)	V390A		Het
Ephb6	C	T	6: 41,590,293 (GRCm39)	A15V	probably benign	Het
Eps15l1	G	T	8: 73,145,762 (GRCm39)	S157*	probably null	Het
Ercc6l2	A	G	13: 63,967,866 (GRCm39)	I155M	possibly damaging	Het
Esco1	G	T	18: 10,594,005 (GRCm39)	T427K	probably benign	Het
Fignl1	C	T	11: 11,752,692 (GRCm39)	C121Y	possibly damaging	Het
Flrt2	A	G	12: 95,746,177 (GRCm39)	S172G	probably damaging	Het
Frmd3	A	T	4: 74,063,269 (GRCm39)		probably null	Het
Fuz	C	T	7: 44,546,721 (GRCm39)	R119W	probably damaging	Het
Gtf2ird2	T	G	5: 134,225,407 (GRCm39)	I165S	probably damaging	Het
Itih1	T	A	14: 30,657,833 (GRCm39)	I417F	probably damaging	Het
Jarid2	G	T	13: 44,994,326 (GRCm39)	V13L		Het
Kndc1	C	T	7: 139,507,708 (GRCm39)	S1222F	possibly damaging	Het
Lcor	C	T	19: 41,573,698 (GRCm39)	R818W	probably damaging	Het
Lpp	T	A	16: 24,580,889 (GRCm39)	W327R	probably benign	Het
Muc16	A	G	9: 18,554,305 (GRCm39)	I3996T	unknown	Het
Myh4	C	A	11: 67,139,573 (GRCm39)	A630E	probably benign	Het
Nxt2	C	T	X: 141,020,747 (GRCm39)	A118V	possibly damaging	Het
Oacyl	T	A	18: 65,840,484 (GRCm39)	F85Y	probably damaging	Het
Or5ac24	C	T	16: 59,165,530 (GRCm39)	C178Y	probably damaging	Het
Or5b118	T	A	19: 13,448,670 (GRCm39)	I70N	probably damaging	Het
Or6c69b	T	A	10: 129,626,727 (GRCm39)	I244F	possibly damaging	Het
Pcdha11	A	C	18: 37,139,877 (GRCm39)	E502A	possibly damaging	Het
Pcnx3	T	C	19: 5,717,582 (GRCm39)	E1509G	possibly damaging	Het
Pfkm	A	T	15: 98,021,680 (GRCm39)	S314C	probably damaging	Het
Plch1	T	C	3: 63,674,924 (GRCm39)	H230R	probably damaging	Het
Ppfia4	A	T	1: 134,251,893 (GRCm39)	V427E	possibly damaging	Het
Ppox	TCTTATACCTGGAC	TC	1: 171,105,447 (GRCm39)		probably benign	Het
Prr11	G	A	11: 86,992,249 (GRCm39)	P209S	probably damaging	Het
Prrc2b	C	A	2: 32,109,304 (GRCm39)	Q1690K	probably damaging	Het
Rgs3	A	T	4: 62,620,465 (GRCm39)	M564L	probably benign	Het
Sec24d	T	C	3: 123,149,452 (GRCm39)	Y820H	probably damaging	Het
Six4	A	C	12: 73,159,498 (GRCm39)	I154S	possibly damaging	Het
Spata31e4	A	T	13: 50,856,276 (GRCm39)	H638L	probably benign	Het
Spock3	A	G	8: 63,801,989 (GRCm39)	K323E	probably damaging	Het
Stmn4	A	T	14: 66,596,126 (GRCm39)		probably null	Het
Tafa2	A	G	10: 123,429,421 (GRCm39)	K12E	probably benign	Het
Tanc2	C	T	11: 105,689,518 (GRCm39)	R227*	probably null	Het
Tcp1	T	A	17: 13,139,210 (GRCm39)	V179D	probably damaging	Het
Trmt5	A	T	12: 73,328,038 (GRCm39)	F388L	probably damaging	Het
Tsc2	G	T	17: 24,822,164 (GRCm39)	P1126H	probably benign	Het
Ttn	T	A	2: 76,612,720 (GRCm39)	I17120F	probably damaging	Het
Ttn	T	G	2: 76,778,270 (GRCm39)	K1372N	unknown	Het
U2surp	T	C	9: 95,359,563 (GRCm39)	T652A	probably benign	Het
Utp20	A	G	10: 88,592,972 (GRCm39)	V2239A	probably benign	Het
Vps33b	A	G	7: 79,935,339 (GRCm39)	T356A	probably damaging	Het
Vwf	C	A	6: 125,623,262 (GRCm39)	H1786Q		Het
Wwox	A	G	8: 115,215,682 (GRCm39)	I152V	probably benign	Het
Ythdc2	A	G	18: 44,977,418 (GRCm39)	T405A	probably benign	Het
Zfpm2	A	T	15: 40,962,712 (GRCm39)	I257F	possibly damaging	Het

Other mutations in Msrb2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Msrb2	APN	2	19,399,152 (GRCm39)	missense	probably damaging	0.98
IGL00330:Msrb2	APN	2	19,376,510 (GRCm39)	missense	unknown
BB002:Msrb2	UTSW	2	19,388,091 (GRCm39)	missense	probably benign	0.06
BB012:Msrb2	UTSW	2	19,388,091 (GRCm39)	missense	probably benign	0.06
R0628:Msrb2	UTSW	2	19,398,091 (GRCm39)	missense	probably damaging	1.00
R1778:Msrb2	UTSW	2	19,388,114 (GRCm39)	missense	probably benign	0.01
R1975:Msrb2	UTSW	2	19,398,032 (GRCm39)	missense	probably damaging	1.00
R6029:Msrb2	UTSW	2	19,399,122 (GRCm39)	missense	probably damaging	1.00
R7925:Msrb2	UTSW	2	19,388,091 (GRCm39)	missense	probably benign	0.06
R7953:Msrb2	UTSW	2	19,399,166 (GRCm39)	makesense	probably null
R9145:Msrb2	UTSW	2	19,399,066 (GRCm39)	missense	probably benign	0.01
R9243:Msrb2	UTSW	2	19,388,073 (GRCm39)	missense	probably benign	0.00
R9607:Msrb2	UTSW	2	19,399,130 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGGTCTGGGATTACTTCTCCAG -3'
(R):5'- AGAGCATGTGTGGCTCTCTG -3'

Sequencing Primer
(F):5'- TTACTTCTCCAGGTGATAGGGCAAAG -3'
(R):5'- TTTCTACAGGTGGATGGAAACC -3'

Posted On

2021-11-19