Incidental Mutation 'R9065:Olfr1474'
ID 689242
Institutional Source Beutler Lab
Gene Symbol Olfr1474
Ensembl Gene ENSMUSG00000096273
Gene Name olfactory receptor 1474
Synonyms MOR202-42, MOR202-26P, GA_x6K02T2RE5P-3803583-3804527
MMRRC Submission
Accession Numbers

Genbank: NM_001011842.1
Essential gene? Probably non essential (E-score: 0.074) question?
Stock # R9065 (G1)
Quality Score 225.009
Status Not validated
Chromosome 19
Chromosomal Location 13469565-13472157 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 13471306 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 70 (I70N)
Ref Sequence ENSEMBL: ENSMUSP00000147143 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000096202] [ENSMUST00000207529] [ENSMUST00000220113]
AlphaFold Q7TQQ8
Predicted Effect probably damaging
Transcript: ENSMUST00000096202
AA Change: I112N

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000093916
Gene: ENSMUSG00000096273
AA Change: I112N

DomainStartEndE-ValueType
Pfam:7tm_4 29 306 1.6e-52 PFAM
Pfam:7TM_GPCR_Srsx 33 303 1e-7 PFAM
Pfam:7tm_1 39 288 8.7e-22 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000207529
AA Change: I70N

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
Predicted Effect probably damaging
Transcript: ENSMUST00000220113
AA Change: I112N

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.7%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI

none
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2900026A02Rik T C 5: 113,175,218 N1472S probably benign Het
4930447C04Rik C A 12: 72,892,830 Q435H possibly damaging Het
Aass G T 6: 23,075,747 A797E probably benign Het
Ap3b1 T A 13: 94,471,715 Y565N probably damaging Het
Arid2 A G 15: 96,371,491 T1162A probably benign Het
Atp11b T C 3: 35,832,982 probably null Het
Card11 G A 5: 140,908,542 P111S probably damaging Het
Ccdc66 T A 14: 27,491,893 E328D probably damaging Het
Cldn8 T A 16: 88,563,014 M8L probably benign Het
Cnot9 T A 1: 74,518,983 L96* probably null Het
Cntnap5c T A 17: 58,138,647 C561S probably damaging Het
Dnah5 A G 15: 28,293,790 D1347G probably benign Het
Epb41 A G 4: 131,955,577 V390A Het
Ephb6 C T 6: 41,613,359 A15V probably benign Het
Eps15l1 G T 8: 72,391,918 S157* probably null Het
Ercc6l2 A G 13: 63,820,052 I155M possibly damaging Het
Esco1 G T 18: 10,594,005 T427K probably benign Het
Fam19a2 A G 10: 123,593,516 K12E probably benign Het
Fignl1 C T 11: 11,802,692 C121Y possibly damaging Het
Flrt2 A G 12: 95,779,403 S172G probably damaging Het
Frmd3 A T 4: 74,145,032 probably null Het
Fuz C T 7: 44,897,297 R119W probably damaging Het
Gm340 C T 19: 41,585,259 R818W probably damaging Het
Gm8765 A T 13: 50,702,240 H638L probably benign Het
Gtf2ird2 T G 5: 134,196,566 I165S probably damaging Het
Itih1 T A 14: 30,935,876 I417F probably damaging Het
Jarid2 G T 13: 44,840,850 V13L Het
Kndc1 C T 7: 139,927,795 S1222F possibly damaging Het
Lpp T A 16: 24,762,139 W327R probably benign Het
Msrb2 T A 2: 19,378,230 F56I possibly damaging Het
Muc16 A G 9: 18,643,009 I3996T unknown Het
Myh4 C A 11: 67,248,747 A630E probably benign Het
Nxt2 C T X: 142,237,751 A118V possibly damaging Het
Oacyl T A 18: 65,707,413 F85Y probably damaging Het
Olfr206 C T 16: 59,345,167 C178Y probably damaging Het
Olfr810 T A 10: 129,790,858 I244F possibly damaging Het
Pcdha11 A C 18: 37,006,824 E502A possibly damaging Het
Pcnx3 T C 19: 5,667,554 E1509G possibly damaging Het
Pfkm A T 15: 98,123,799 S314C probably damaging Het
Plch1 T C 3: 63,767,503 H230R probably damaging Het
Ppfia4 A T 1: 134,324,155 V427E possibly damaging Het
Ppox TCTTATACCTGGAC TC 1: 171,277,874 probably benign Het
Prr11 G A 11: 87,101,423 P209S probably damaging Het
Prrc2b C A 2: 32,219,292 Q1690K probably damaging Het
Rgs3 A T 4: 62,702,228 M564L probably benign Het
Sec24d T C 3: 123,355,803 Y820H probably damaging Het
Six4 A C 12: 73,112,724 I154S possibly damaging Het
Spock3 A G 8: 63,348,955 K323E probably damaging Het
Stmn4 A T 14: 66,358,677 probably null Het
Tanc2 C T 11: 105,798,692 R227* probably null Het
Tcp1 T A 17: 12,920,323 V179D probably damaging Het
Trmt5 A T 12: 73,281,264 F388L probably damaging Het
Tsc2 G T 17: 24,603,190 P1126H probably benign Het
Ttn T A 2: 76,782,376 I17120F probably damaging Het
Ttn T G 2: 76,947,926 K1372N unknown Het
U2surp T C 9: 95,477,510 T652A probably benign Het
Utp20 A G 10: 88,757,110 V2239A probably benign Het
Vps33b A G 7: 80,285,591 T356A probably damaging Het
Vwf C A 6: 125,646,299 H1786Q Het
Wwox A G 8: 114,488,942 I152V probably benign Het
Ythdc2 A G 18: 44,844,351 T405A probably benign Het
Zfpm2 A T 15: 41,099,316 I257F possibly damaging Het
Other mutations in Olfr1474
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03256:Olfr1474 APN 19 13471267 missense probably damaging 0.99
D605:Olfr1474 UTSW 19 13471157 nonsense probably null
R0173:Olfr1474 UTSW 19 13471701 missense probably benign 0.02
R1102:Olfr1474 UTSW 19 13471407 missense probably damaging 0.97
R1515:Olfr1474 UTSW 19 13471680 missense probably damaging 0.97
R1780:Olfr1474 UTSW 19 13471362 missense probably benign 0.14
R2061:Olfr1474 UTSW 19 13471241 missense probably damaging 0.98
R4016:Olfr1474 UTSW 19 13471197 missense possibly damaging 0.95
R4485:Olfr1474 UTSW 19 13471555 missense probably benign 0.08
R5119:Olfr1474 UTSW 19 13471546 missense probably benign 0.00
R5150:Olfr1474 UTSW 19 13471430 missense probably benign 0.01
R5156:Olfr1474 UTSW 19 13471673 missense probably damaging 1.00
R5699:Olfr1474 UTSW 19 13470972 start codon destroyed probably null 0.78
R5800:Olfr1474 UTSW 19 13471896 missense probably benign 0.06
R5840:Olfr1474 UTSW 19 13471878 missense probably benign 0.01
R5953:Olfr1474 UTSW 19 13471368 missense possibly damaging 0.92
R5997:Olfr1474 UTSW 19 13471506 missense probably benign 0.12
R6233:Olfr1474 UTSW 19 13471740 missense probably damaging 1.00
R6488:Olfr1474 UTSW 19 13471617 missense probably damaging 1.00
R6847:Olfr1474 UTSW 19 13471038 missense probably benign 0.03
R6964:Olfr1474 UTSW 19 13471361 nonsense probably null
R7214:Olfr1474 UTSW 19 13470973 start codon destroyed probably null 1.00
R8001:Olfr1474 UTSW 19 13471422 missense probably benign 0.03
R8035:Olfr1474 UTSW 19 13471899 missense probably benign
R8129:Olfr1474 UTSW 19 13471144 missense probably damaging 1.00
R9018:Olfr1474 UTSW 19 13471357 missense possibly damaging 0.60
R9061:Olfr1474 UTSW 19 13471159 missense probably damaging 0.98
R9373:Olfr1474 UTSW 19 13471852 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ATACTGATTCTGCTGGACTCCC -3'
(R):5'- TGTGTGGGTGTCAGAACAAG -3'

Sequencing Primer
(F):5'- GATTCTGCTGGACTCCCGACTC -3'
(R):5'- TCTAGCAGTGGGGGAATGTCAC -3'
Posted On 2021-11-19