Mutagenetix > Incidental Mutation

Incidental Mutation 'R9079:Wdr90'

689921

Institutional Source

Beutler Lab

Gene Symbol

Wdr90

Ensembl Gene

ENSMUSG00000073434

Gene Name

WD repeat domain 90

Synonyms

3230401M21Rik

MMRRC Submission

068899-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R9079 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

26063745-26080475 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 26076403 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 45 (W45R)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079461] [ENSMUST00000176923]

AlphaFold

Q6ZPG2

Predicted Effect

probably damaging
Transcript: ENSMUST00000079461
AA Change: W457R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000078426
Gene: ENSMUSG00000073434
AA Change: W457R

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	183	5e-26	PFAM
Pfam:DUF667	210	271	3.7e-9	PFAM
low complexity region	381	396	N/A	INTRINSIC
WD40	478	519	5.94e0	SMART
WD40	522	565	3.2e0	SMART
WD40	572	612	3.3e1	SMART
WD40	687	725	1.15e1	SMART
WD40	728	766	5.75e-1	SMART
WD40	768	808	9.24e-4	SMART
WD40	811	850	4.13e0	SMART
WD40	853	892	4.62e-1	SMART
WD40	950	993	1.07e1	SMART
WD40	996	1035	5.75e-1	SMART
WD40	1040	1077	1.58e-2	SMART
WD40	1290	1334	5.23e-3	SMART
WD40	1337	1378	1.27e-1	SMART
WD40	1384	1419	1.83e2	SMART
WD40	1422	1469	3.08e0	SMART
WD40	1472	1509	9.9e0	SMART
WD40	1568	1607	9.02e-7	SMART
WD40	1610	1655	5.75e-1	SMART
WD40	1659	1697	2.98e-1	SMART
WD40	1700	1749	6.14e1	SMART
WD40	1850	1888	1.92e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176923
AA Change: W439R

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000135420
Gene: ENSMUSG00000073434
AA Change: W439R

Domain	Start	End	E-Value	Type
low complexity region	32	53	N/A	INTRINSIC
Pfam:DUF667	60	252	5.1e-14	PFAM
low complexity region	363	378	N/A	INTRINSIC
WD40	460	501	5.94e0	SMART
WD40	504	547	3.2e0	SMART
WD40	554	594	3.3e1	SMART
WD40	669	707	1.15e1	SMART
WD40	710	748	5.75e-1	SMART
WD40	750	790	9.24e-4	SMART
WD40	793	832	4.13e0	SMART
WD40	835	874	4.62e-1	SMART
WD40	932	975	1.07e1	SMART
WD40	978	1017	5.75e-1	SMART
WD40	1022	1059	1.58e-2	SMART
WD40	1272	1316	5.23e-3	SMART
WD40	1319	1360	1.27e-1	SMART
WD40	1366	1401	1.83e2	SMART
WD40	1404	1451	3.08e0	SMART
WD40	1454	1491	9.9e0	SMART
WD40	1550	1589	9.02e-7	SMART
WD40	1592	1637	5.75e-1	SMART
WD40	1641	1679	2.98e-1	SMART
WD40	1682	1731	6.14e1	SMART
WD40	1832	1870	1.92e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atad2	A	T	15: 57,989,223 (GRCm39)	S212R	probably benign	Het
Bcl9	A	T	3: 97,112,816 (GRCm39)	V1213E	probably damaging	Het
Brd1	T	C	15: 88,598,153 (GRCm39)	D531G	probably damaging	Het
Cd1d1	T	A	3: 86,906,197 (GRCm39)	Y26F	probably benign	Het
Cep83	T	C	10: 94,564,541 (GRCm39)	F160S	possibly damaging	Het
Cfap61	G	T	2: 145,781,859 (GRCm39)	V31F	probably benign	Het
Cox11	G	A	11: 90,535,246 (GRCm39)	M232I	probably damaging	Het
Cpne7	C	T	8: 123,856,951 (GRCm39)	P402L	probably damaging	Het
Cubn	A	G	2: 13,291,914 (GRCm39)	S3304P	probably benign	Het
Eif4b	T	A	15: 102,003,177 (GRCm39)	D549E	unknown	Het
Extl1	T	A	4: 134,089,975 (GRCm39)	N378Y	probably damaging	Het
Fbxo4	C	T	15: 3,998,388 (GRCm39)		probably null	Het
Fhip1a	C	A	3: 85,579,590 (GRCm39)	G872*	probably null	Het
G430095P16Rik	A	T	8: 85,453,412 (GRCm39)	H133L	unknown	Het
Gm8138	A	T	14: 43,272,502 (GRCm39)	N104K		Het
Gzma	A	G	13: 113,232,858 (GRCm39)	F78S	probably benign	Het
Hars2	T	C	18: 36,923,190 (GRCm39)	S400P	possibly damaging	Het
Hnrnpm	G	T	17: 33,868,775 (GRCm39)	R551S	probably damaging	Het
Htr5b	T	A	1: 121,455,816 (GRCm39)	T35S	probably benign	Het
Jak3	A	C	8: 72,131,898 (GRCm39)	E158A	probably benign	Het
Kctd16	A	G	18: 40,390,080 (GRCm39)		probably benign	Het
Kdm4c	C	A	4: 74,277,738 (GRCm39)	D797E	probably benign	Het
Klhl31	T	A	9: 77,558,151 (GRCm39)	V289E	probably damaging	Het
Loxhd1	T	C	18: 77,490,593 (GRCm39)	S1398P	probably benign	Het
Matn4	A	T	2: 164,235,473 (GRCm39)		probably benign	Het
Mrps28	A	G	3: 8,867,308 (GRCm39)	*187Q	probably null	Het
Myh7b	G	A	2: 155,465,174 (GRCm39)	V677I	probably damaging	Het
Naip1	A	T	13: 100,559,727 (GRCm39)	D1092E	probably benign	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Notch3	A	T	17: 32,383,033 (GRCm39)		probably benign	Het
Nr1h2	C	A	7: 44,199,430 (GRCm39)	E440D	possibly damaging	Het
Or1l4b	T	C	2: 37,036,989 (GRCm39)	V255A	probably damaging	Het
Or6c207	A	T	10: 129,104,466 (GRCm39)	M242K	possibly damaging	Het
Or7g16	A	T	9: 18,726,731 (GRCm39)	N286K	probably damaging	Het
Or8g2b	T	A	9: 39,750,769 (GRCm39)	I13N	probably benign	Het
Osbpl9	A	T	4: 108,920,644 (GRCm39)	V543D	possibly damaging	Het
Pcgf6	T	C	19: 47,039,053 (GRCm39)	E69G	possibly damaging	Het
Piezo2	A	G	18: 63,157,537 (GRCm39)	L2391P	probably damaging	Het
Rcn2	T	A	9: 55,952,393 (GRCm39)		probably benign	Het
Ripor2	G	A	13: 24,915,637 (GRCm39)	R1069H	probably benign	Het
Ro60	A	C	1: 143,641,519 (GRCm39)	L314R	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Senp5	A	T	16: 31,787,718 (GRCm39)	I635N	probably damaging	Het
Slc30a9	A	T	5: 67,484,241 (GRCm39)	K126M	possibly damaging	Het
Slc9b2	A	T	3: 135,042,150 (GRCm39)	R476S	probably damaging	Het
Sptb	G	T	12: 76,677,454 (GRCm39)	R127S	probably damaging	Het
Svil	T	C	18: 5,056,308 (GRCm39)	S394P	probably benign	Het
Tjp1	A	T	7: 64,950,966 (GRCm39)	I1636N	possibly damaging	Het
Tlcd5	C	T	9: 43,022,664 (GRCm39)	R230Q	probably benign	Het
Trio	T	C	15: 27,733,023 (GRCm39)	I2953V	possibly damaging	Het
Ttn	A	G	2: 76,632,734 (GRCm39)	F14107L	probably damaging	Het
Usp20	A	G	2: 30,895,120 (GRCm39)		probably benign	Het
Vmn1r123	A	T	7: 20,896,979 (GRCm39)	L290F	probably benign	Het
Vmn1r202	T	C	13: 22,685,602 (GRCm39)	M272V	probably benign	Het
Vmn2r97	A	T	17: 19,149,640 (GRCm39)	N343Y	probably benign	Het

Other mutations in Wdr90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Wdr90	APN	17	26,068,338 (GRCm39)	missense	probably damaging	1.00
IGL01118:Wdr90	APN	17	26,073,661 (GRCm39)	missense	probably damaging	1.00
IGL01964:Wdr90	APN	17	26,067,383 (GRCm39)	missense	probably benign
IGL02116:Wdr90	APN	17	26,078,466 (GRCm39)	missense	probably benign	0.12
IGL02172:Wdr90	APN	17	26,069,408 (GRCm39)	missense	probably benign	0.22
IGL02716:Wdr90	APN	17	26,076,194 (GRCm39)	missense	probably damaging	0.96
IGL02961:Wdr90	APN	17	26,067,649 (GRCm39)	nonsense	probably null
IGL03229:Wdr90	APN	17	26,064,437 (GRCm39)	splice site	probably benign
IGL03367:Wdr90	APN	17	26,066,765 (GRCm39)	splice site	probably benign
IGL03098:Wdr90	UTSW	17	26,078,961 (GRCm39)	intron	probably benign
R0111:Wdr90	UTSW	17	26,067,418 (GRCm39)	splice site	probably benign
R0454:Wdr90	UTSW	17	26,079,023 (GRCm39)	missense	probably damaging	0.96
R0457:Wdr90	UTSW	17	26,079,459 (GRCm39)	missense	probably benign	0.00
R0488:Wdr90	UTSW	17	26,067,591 (GRCm39)	missense	probably damaging	1.00
R0622:Wdr90	UTSW	17	26,074,632 (GRCm39)	missense	probably damaging	1.00
R0671:Wdr90	UTSW	17	26,065,367 (GRCm39)	missense	probably benign	0.04
R0799:Wdr90	UTSW	17	26,079,104 (GRCm39)	missense	probably benign	0.38
R1177:Wdr90	UTSW	17	26,065,028 (GRCm39)	missense	possibly damaging	0.85
R1460:Wdr90	UTSW	17	26,079,422 (GRCm39)	missense	possibly damaging	0.82
R1468:Wdr90	UTSW	17	26,073,027 (GRCm39)	missense	probably damaging	1.00
R1468:Wdr90	UTSW	17	26,073,027 (GRCm39)	missense	probably damaging	1.00
R1544:Wdr90	UTSW	17	26,068,284 (GRCm39)	missense	possibly damaging	0.69
R2057:Wdr90	UTSW	17	26,074,173 (GRCm39)	missense	probably benign	0.05
R2087:Wdr90	UTSW	17	26,065,577 (GRCm39)	missense	probably damaging	1.00
R2159:Wdr90	UTSW	17	26,070,715 (GRCm39)	missense	probably benign
R2208:Wdr90	UTSW	17	26,079,362 (GRCm39)	missense	probably damaging	1.00
R2345:Wdr90	UTSW	17	26,078,136 (GRCm39)	missense	probably benign	0.05
R2391:Wdr90	UTSW	17	26,070,429 (GRCm39)	missense	probably damaging	1.00
R2394:Wdr90	UTSW	17	26,070,429 (GRCm39)	missense	probably damaging	1.00
R2520:Wdr90	UTSW	17	26,074,326 (GRCm39)	missense	probably damaging	1.00
R3798:Wdr90	UTSW	17	26,069,472 (GRCm39)	missense	probably benign	0.01
R3979:Wdr90	UTSW	17	26,078,252 (GRCm39)	missense	probably benign	0.00
R4111:Wdr90	UTSW	17	26,068,342 (GRCm39)	missense	possibly damaging	0.94
R4326:Wdr90	UTSW	17	26,072,705 (GRCm39)	missense	probably benign	0.25
R4459:Wdr90	UTSW	17	26,079,976 (GRCm39)	missense	possibly damaging	0.95
R4697:Wdr90	UTSW	17	26,074,337 (GRCm39)	missense	probably benign	0.03
R4735:Wdr90	UTSW	17	26,078,424 (GRCm39)	missense	probably benign
R4907:Wdr90	UTSW	17	26,079,624 (GRCm39)	intron	probably benign
R5070:Wdr90	UTSW	17	26,065,307 (GRCm39)	missense	probably damaging	1.00
R5230:Wdr90	UTSW	17	26,074,277 (GRCm39)	missense	probably benign	0.01
R5268:Wdr90	UTSW	17	26,069,819 (GRCm39)	missense	probably damaging	1.00
R5287:Wdr90	UTSW	17	26,080,441 (GRCm39)	utr 5 prime	probably benign
R5382:Wdr90	UTSW	17	26,064,572 (GRCm39)	missense	probably damaging	1.00
R5511:Wdr90	UTSW	17	26,063,995 (GRCm39)	unclassified	probably benign
R5545:Wdr90	UTSW	17	26,064,830 (GRCm39)	missense	probably damaging	1.00
R5707:Wdr90	UTSW	17	26,076,166 (GRCm39)	missense	probably benign	0.00
R5973:Wdr90	UTSW	17	26,065,381 (GRCm39)	missense	probably damaging	1.00
R5973:Wdr90	UTSW	17	26,064,107 (GRCm39)	missense	probably damaging	0.99
R6385:Wdr90	UTSW	17	26,067,504 (GRCm39)	missense	probably damaging	1.00
R6481:Wdr90	UTSW	17	26,064,885 (GRCm39)	missense	probably damaging	0.99
R7078:Wdr90	UTSW	17	26,068,623 (GRCm39)	missense	probably damaging	1.00
R7214:Wdr90	UTSW	17	26,064,367 (GRCm39)	missense	probably benign	0.00
R7288:Wdr90	UTSW	17	26,065,286 (GRCm39)	missense	probably benign	0.03
R7304:Wdr90	UTSW	17	26,070,480 (GRCm39)	missense	probably benign	0.10
R7309:Wdr90	UTSW	17	26,079,676 (GRCm39)	missense	probably benign	0.02
R7391:Wdr90	UTSW	17	26,065,502 (GRCm39)	missense	probably benign	0.08
R7622:Wdr90	UTSW	17	26,073,083 (GRCm39)	missense	probably benign	0.00
R7646:Wdr90	UTSW	17	26,079,104 (GRCm39)	missense	probably benign	0.38
R7772:Wdr90	UTSW	17	26,080,465 (GRCm39)	start gained	probably benign
R7779:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7780:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7781:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7782:Wdr90	UTSW	17	26,065,300 (GRCm39)	missense	probably damaging	1.00
R7812:Wdr90	UTSW	17	26,071,532 (GRCm39)	missense	probably damaging	1.00
R7870:Wdr90	UTSW	17	26,079,513 (GRCm39)	missense	probably damaging	0.96
R7911:Wdr90	UTSW	17	26,069,723 (GRCm39)	missense	probably benign	0.00
R8126:Wdr90	UTSW	17	26,067,951 (GRCm39)	missense	possibly damaging	0.48
R8260:Wdr90	UTSW	17	26,064,141 (GRCm39)	missense	probably damaging	1.00
R8315:Wdr90	UTSW	17	26,064,399 (GRCm39)	missense	probably benign	0.21
R8919:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R8938:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R8940:Wdr90	UTSW	17	26,076,146 (GRCm39)	missense
R9038:Wdr90	UTSW	17	26,076,200 (GRCm39)	missense
R9401:Wdr90	UTSW	17	26,064,750 (GRCm39)	small insertion	probably benign
R9471:Wdr90	UTSW	17	26,080,015 (GRCm39)	missense	possibly damaging	0.72
R9642:Wdr90	UTSW	17	26,072,694 (GRCm39)	critical splice donor site	probably null
X0064:Wdr90	UTSW	17	26,067,537 (GRCm39)	missense	probably damaging	1.00
Z1176:Wdr90	UTSW	17	26,079,470 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAGCGTATCACTGCCATTGAG -3'
(R):5'- AGGCTTTAGTTGATTTCTGCAC -3'

Sequencing Primer
(F):5'- ACTGCCATTGAGCGCCAG -3'
(R):5'- GCACATATGCCTCTGTGTGTG -3'

Posted On

2021-11-19