Incidental Mutation 'R8927:Prag1'
ID 690159
Institutional Source Beutler Lab
Gene Symbol Prag1
Ensembl Gene ENSMUSG00000050271
Gene Name PEAK1 related kinase activating pseudokinase 1
Synonyms D8Ertd82e, NACK
MMRRC Submission 068771-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8927 (G1)
Quality Score 225.009
Status Validated
Chromosome 8
Chromosomal Location 36561982-36614941 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to G at 36614360 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Glycine at position 1304 (V1304G)
Ref Sequence ENSEMBL: ENSMUSP00000106118 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000110492]
AlphaFold Q571I4
Predicted Effect probably damaging
Transcript: ENSMUST00000110492
AA Change: V1304G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000106118
Gene: ENSMUSG00000050271
AA Change: V1304G

DomainStartEndE-ValueType
low complexity region 317 333 N/A INTRINSIC
low complexity region 395 412 N/A INTRINSIC
low complexity region 525 544 N/A INTRINSIC
low complexity region 781 793 N/A INTRINSIC
low complexity region 892 925 N/A INTRINSIC
Pfam:Pkinase_Tyr 1060 1288 1.7e-7 PFAM
Pfam:Pkinase 1061 1293 1.5e-13 PFAM
low complexity region 1363 1373 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.9%
Validation Efficiency 99% (78/79)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous disruption of this gene leads to complete embryonic lethality. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 86 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A630089N07Rik A T 16: 97,867,367 (GRCm39) H198Q probably damaging Het
Abcb9 T C 5: 124,221,706 (GRCm39) T223A probably benign Het
Actc1 T A 2: 113,880,881 (GRCm39) K115* probably null Het
Angptl2 G A 2: 33,132,316 (GRCm39) D308N probably benign Het
Arl4d A C 11: 101,557,892 (GRCm39) Q139H possibly damaging Het
Aspm T A 1: 139,418,125 (GRCm39) C2951* probably null Het
Atp23 A T 10: 126,723,362 (GRCm39) *247R probably null Het
B3gnt7 T A 1: 86,232,839 (GRCm39) S28R probably benign Het
Bmp7 C T 2: 172,721,211 (GRCm39) R288H probably damaging Het
Bsdc1 G T 4: 129,355,439 (GRCm39) probably benign Het
Capn13 T C 17: 73,631,761 (GRCm39) probably null Het
Ccdc146 T C 5: 21,538,060 (GRCm39) E88G probably damaging Het
Ccdc73 C A 2: 104,822,542 (GRCm39) D2E Het
Cdc5l C A 17: 45,721,839 (GRCm39) R477L Het
Cdh22 T A 2: 164,965,504 (GRCm39) H529L possibly damaging Het
Chchd3 T A 6: 32,780,951 (GRCm39) E178D probably benign Het
Ciz1 A T 2: 32,257,512 (GRCm39) R186* probably null Het
Col4a2 A G 8: 11,475,543 (GRCm39) probably null Het
Copa T A 1: 171,931,737 (GRCm39) H329Q probably null Het
Dcaf7 A G 11: 105,942,752 (GRCm39) D233G probably damaging Het
Dclk2 G T 3: 86,739,048 (GRCm39) P317Q probably damaging Het
Dgat2 T C 7: 98,818,710 (GRCm39) Y90C probably benign Het
Dsc3 A T 18: 20,107,234 (GRCm39) H462Q probably benign Het
Fam151a T A 4: 106,603,242 (GRCm39) probably benign Het
Fn1 T C 1: 71,638,535 (GRCm39) N1998S probably benign Het
Fpr2 T C 17: 18,113,724 (GRCm39) L240S possibly damaging Het
Fzd9 A T 5: 135,278,589 (GRCm39) M432K probably damaging Het
Gm5862 T A 5: 26,226,678 (GRCm39) I75F probably damaging Het
Gpr45 T A 1: 43,072,314 (GRCm39) V319D probably damaging Het
H4c11 T C 13: 21,919,525 (GRCm39) V87A possibly damaging Het
Igfn1 T A 1: 135,905,984 (GRCm39) R275W probably damaging Het
Inafm1 G A 7: 16,006,980 (GRCm39) A79V unknown Het
Itfg1 C T 8: 86,567,420 (GRCm39) probably benign Het
Kcnt2 T A 1: 140,356,535 (GRCm39) probably null Het
Klhl35 T G 7: 99,120,221 (GRCm39) I366S probably damaging Het
Lysmd1 A T 3: 95,045,831 (GRCm39) I223F probably damaging Het
Mrps30 A G 13: 118,523,205 (GRCm39) L189P probably damaging Het
Ms4a4d A C 19: 11,533,574 (GRCm39) T152P probably benign Het
Mtcl1 A T 17: 66,755,628 (GRCm39) I238K probably benign Het
Musk T C 4: 58,301,638 (GRCm39) I132T probably damaging Het
Myh2 A T 11: 67,079,509 (GRCm39) M989L probably benign Het
Myh8 G A 11: 67,174,081 (GRCm39) R172H probably benign Het
Nt5c1a C T 4: 123,102,281 (GRCm39) T69M possibly damaging Het
Nuak2 T A 1: 132,255,916 (GRCm39) D196E probably damaging Het
Or52h7 A T 7: 104,214,229 (GRCm39) H267L probably damaging Het
Or5ap2 A T 2: 85,679,918 (GRCm39) I41F possibly damaging Het
Or5t7 T C 2: 86,507,630 (GRCm39) T16A probably benign Het
Or8k53 T C 2: 86,178,090 (GRCm39) T7A possibly damaging Het
Or8s10 A G 15: 98,336,519 (GRCm39) probably benign Het
Palb2 A T 7: 121,723,821 (GRCm39) C643S probably damaging Het
Pde5a T A 3: 122,633,249 (GRCm39) I706N probably damaging Het
Pdlim4 T C 11: 53,950,790 (GRCm39) R100G probably benign Het
Pip5k1c T A 10: 81,128,906 (GRCm39) V5E possibly damaging Het
Pira1 A C 7: 3,742,358 (GRCm39) N56K probably benign Het
Plekhm1 A G 11: 103,268,039 (GRCm39) V644A probably benign Het
Plk2 T G 13: 110,535,750 (GRCm39) V524G probably damaging Het
Ppl T A 16: 4,905,474 (GRCm39) N1607I probably benign Het
Prex1 C T 2: 166,426,995 (GRCm39) V1077M probably damaging Het
Prmt6 T C 3: 110,158,248 (GRCm39) S14G probably benign Het
Psrc1 T G 3: 108,293,973 (GRCm39) S263R probably damaging Het
Qrfprl C A 6: 65,358,597 (GRCm39) S107* probably null Het
Rbp7 A T 4: 149,537,958 (GRCm39) L36* probably null Het
Rgs12 G T 5: 35,123,633 (GRCm39) W472L possibly damaging Het
Rrp8 C A 7: 105,384,073 (GRCm39) C143F possibly damaging Het
Sart1 T C 19: 5,438,529 (GRCm39) S43G probably benign Het
Setx A G 2: 29,016,971 (GRCm39) T25A possibly damaging Het
Skint5 G A 4: 113,341,099 (GRCm39) T1414I probably benign Het
Sorbs2 T A 8: 46,248,952 (GRCm39) S734R probably damaging Het
Spata31g1 A T 4: 42,972,251 (GRCm39) Y528F probably benign Het
Spon1 T C 7: 113,629,592 (GRCm39) probably null Het
Sptbn1 C T 11: 30,088,962 (GRCm39) V767I probably damaging Het
Srf T C 17: 46,860,095 (GRCm39) I477V probably damaging Het
Tanc2 A G 11: 105,701,331 (GRCm39) Y282C probably damaging Het
Tbc1d23 A G 16: 56,992,152 (GRCm39) Y603H probably damaging Het
Tdpoz6 T A 3: 93,599,950 (GRCm39) T140S probably benign Het
Tfap4 T C 16: 4,369,218 (GRCm39) D134G probably damaging Het
Tmem200c A G 17: 69,148,733 (GRCm39) T439A probably benign Het
Tmem63b A T 17: 45,975,908 (GRCm39) M515K probably damaging Het
Tnc A T 4: 63,925,595 (GRCm39) N980K probably damaging Het
Ttc23l T A 15: 10,530,720 (GRCm39) L297F probably damaging Het
Vinac1 A G 2: 128,882,789 (GRCm39) S76P Het
Vmn1r170 A G 7: 23,305,814 (GRCm39) N72S possibly damaging Het
Vmn2r8 T C 5: 108,950,131 (GRCm39) M239V Het
Vopp1 A G 6: 57,731,578 (GRCm39) F138L probably damaging Het
Vps41 T A 13: 18,929,501 (GRCm39) F79I probably benign Het
Zfp317 T C 9: 19,552,521 (GRCm39) M1T probably null Het
Other mutations in Prag1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01107:Prag1 APN 8 36,567,085 (GRCm39) missense probably benign 0.01
IGL01132:Prag1 APN 8 36,613,511 (GRCm39) missense probably damaging 1.00
IGL01322:Prag1 APN 8 36,571,088 (GRCm39) missense probably benign 0.01
IGL01343:Prag1 APN 8 36,570,200 (GRCm39) missense possibly damaging 0.95
IGL01726:Prag1 APN 8 36,570,146 (GRCm39) missense probably damaging 1.00
IGL01739:Prag1 APN 8 36,569,834 (GRCm39) missense probably benign 0.00
IGL02420:Prag1 APN 8 36,614,580 (GRCm39) utr 3 prime probably benign
IGL02433:Prag1 APN 8 36,606,722 (GRCm39) missense probably damaging 1.00
IGL02627:Prag1 APN 8 36,606,593 (GRCm39) missense possibly damaging 0.93
IGL02797:Prag1 APN 8 36,606,655 (GRCm39) missense probably damaging 1.00
IGL03070:Prag1 APN 8 36,570,703 (GRCm39) missense probably benign 0.01
IGL03323:Prag1 APN 8 36,607,162 (GRCm39) missense probably damaging 1.00
FR4340:Prag1 UTSW 8 36,571,040 (GRCm39) small insertion probably benign
FR4548:Prag1 UTSW 8 36,571,039 (GRCm39) small insertion probably benign
FR4589:Prag1 UTSW 8 36,571,037 (GRCm39) small insertion probably benign
FR4976:Prag1 UTSW 8 36,571,037 (GRCm39) small insertion probably benign
R0325:Prag1 UTSW 8 36,570,958 (GRCm39) missense probably benign 0.00
R0486:Prag1 UTSW 8 36,613,787 (GRCm39) missense probably damaging 1.00
R0506:Prag1 UTSW 8 36,570,854 (GRCm39) missense possibly damaging 0.92
R0507:Prag1 UTSW 8 36,571,277 (GRCm39) missense probably damaging 1.00
R0595:Prag1 UTSW 8 36,614,156 (GRCm39) missense probably damaging 1.00
R0618:Prag1 UTSW 8 36,566,987 (GRCm39) missense probably damaging 1.00
R0618:Prag1 UTSW 8 36,566,987 (GRCm39) missense probably damaging 1.00
R0885:Prag1 UTSW 8 36,570,421 (GRCm39) missense probably benign 0.00
R1015:Prag1 UTSW 8 36,613,697 (GRCm39) missense probably damaging 1.00
R1168:Prag1 UTSW 8 36,613,799 (GRCm39) missense probably damaging 1.00
R1182:Prag1 UTSW 8 36,614,413 (GRCm39) missense possibly damaging 0.95
R1227:Prag1 UTSW 8 36,607,105 (GRCm39) missense probably damaging 1.00
R1282:Prag1 UTSW 8 36,567,068 (GRCm39) missense probably damaging 0.96
R1469:Prag1 UTSW 8 36,613,452 (GRCm39) splice site probably benign
R1656:Prag1 UTSW 8 36,571,500 (GRCm39) missense probably damaging 1.00
R1660:Prag1 UTSW 8 36,607,177 (GRCm39) missense possibly damaging 0.73
R1676:Prag1 UTSW 8 36,570,052 (GRCm39) missense probably damaging 0.96
R1820:Prag1 UTSW 8 36,570,958 (GRCm39) missense probably benign 0.00
R1970:Prag1 UTSW 8 36,596,314 (GRCm39) splice site probably null
R1974:Prag1 UTSW 8 36,570,081 (GRCm39) missense probably damaging 1.00
R4398:Prag1 UTSW 8 36,570,809 (GRCm39) missense probably damaging 1.00
R4429:Prag1 UTSW 8 36,613,796 (GRCm39) missense probably damaging 1.00
R4627:Prag1 UTSW 8 36,570,446 (GRCm39) missense probably damaging 1.00
R4980:Prag1 UTSW 8 36,606,740 (GRCm39) missense probably damaging 1.00
R5131:Prag1 UTSW 8 36,607,123 (GRCm39) missense probably damaging 1.00
R5215:Prag1 UTSW 8 36,567,043 (GRCm39) missense probably benign 0.06
R5346:Prag1 UTSW 8 36,570,839 (GRCm39) missense probably damaging 1.00
R5414:Prag1 UTSW 8 36,606,776 (GRCm39) missense probably benign 0.00
R5535:Prag1 UTSW 8 36,571,168 (GRCm39) missense probably benign
R5687:Prag1 UTSW 8 36,613,967 (GRCm39) missense probably benign 0.02
R5785:Prag1 UTSW 8 36,570,641 (GRCm39) missense probably benign 0.35
R5817:Prag1 UTSW 8 36,570,857 (GRCm39) missense probably damaging 1.00
R6002:Prag1 UTSW 8 36,571,337 (GRCm39) missense probably benign 0.31
R6127:Prag1 UTSW 8 36,614,555 (GRCm39) missense unknown
R6240:Prag1 UTSW 8 36,570,506 (GRCm39) missense probably benign 0.03
R6277:Prag1 UTSW 8 36,613,745 (GRCm39) missense probably damaging 1.00
R6326:Prag1 UTSW 8 36,569,860 (GRCm39) missense possibly damaging 0.79
R6741:Prag1 UTSW 8 36,614,434 (GRCm39) missense probably benign 0.41
R6925:Prag1 UTSW 8 36,571,048 (GRCm39) missense probably damaging 1.00
R7085:Prag1 UTSW 8 36,571,391 (GRCm39) missense possibly damaging 0.71
R7095:Prag1 UTSW 8 36,569,714 (GRCm39) missense probably benign
R7204:Prag1 UTSW 8 36,613,915 (GRCm39) missense probably benign 0.03
R7213:Prag1 UTSW 8 36,613,769 (GRCm39) missense probably damaging 0.99
R7567:Prag1 UTSW 8 36,569,760 (GRCm39) missense possibly damaging 0.68
R7577:Prag1 UTSW 8 36,614,096 (GRCm39) missense probably damaging 1.00
R7783:Prag1 UTSW 8 36,570,409 (GRCm39) missense possibly damaging 0.66
R8146:Prag1 UTSW 8 36,571,364 (GRCm39) missense probably damaging 1.00
R8152:Prag1 UTSW 8 36,567,079 (GRCm39) missense possibly damaging 0.53
R8157:Prag1 UTSW 8 36,614,393 (GRCm39) missense probably damaging 0.99
R8332:Prag1 UTSW 8 36,613,457 (GRCm39) missense probably damaging 1.00
R8821:Prag1 UTSW 8 36,613,891 (GRCm39) missense probably benign
R8831:Prag1 UTSW 8 36,613,891 (GRCm39) missense probably benign
R8928:Prag1 UTSW 8 36,614,360 (GRCm39) missense probably damaging 1.00
R8973:Prag1 UTSW 8 36,566,744 (GRCm39) start gained probably benign
R9516:Prag1 UTSW 8 36,607,208 (GRCm39) missense probably damaging 1.00
R9596:Prag1 UTSW 8 36,570,113 (GRCm39) missense probably damaging 1.00
R9598:Prag1 UTSW 8 36,571,069 (GRCm39) missense probably benign 0.20
Z1177:Prag1 UTSW 8 36,614,296 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCATCTATGAGCTGCTCCACCAG -3'
(R):5'- GTTGTAGGAGCTTCAGGGAC -3'

Sequencing Primer
(F):5'- AACCCATTCGAGGTGCG -3'
(R):5'- ACTGCAAGAGGGCCCCTG -3'
Posted On 2021-11-19