Mutagenetix > Incidental Mutation

Incidental Mutation 'R9083:Zfp932'

690389

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

068902-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R9083 (G1)

Quality Score

221.009

Status

Not validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 110157100 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 266 (H266L)

Ref Sequence

ENSEMBL: ENSMUSP00000108159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000112536
AA Change: H233L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: H233L

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000112540
AA Change: H266L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: H266L

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000187241
AA Change: H266L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: H266L

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.5%

Validation Efficiency

100% (61/61)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	T	6: 128,534,524 (GRCm39)	S844R	possibly damaging	Het
Adig	C	A	2: 158,347,709 (GRCm39)		probably benign	Het
Aspm	A	G	1: 139,421,436 (GRCm39)	N3106S	possibly damaging	Het
Bcas1	A	T	2: 170,190,081 (GRCm39)		probably benign	Het
Bco1	A	C	8: 117,844,143 (GRCm39)	I286L	probably benign	Het
Bnc1	C	T	7: 81,624,646 (GRCm39)	V194I	probably benign	Het
Bptf	G	A	11: 106,959,176 (GRCm39)	R1912W	probably damaging	Het
Cacna1a	A	G	8: 85,344,511 (GRCm39)	I1858V	probably benign	Het
Chdh	T	C	14: 29,753,703 (GRCm39)	F204S	probably damaging	Het
Cic	A	G	7: 24,985,470 (GRCm39)	T1212A	probably damaging	Het
Cpne7	C	T	8: 123,856,951 (GRCm39)	P402L	probably damaging	Het
Cts8	A	G	13: 61,397,036 (GRCm39)	Y295H	probably damaging	Het
Cyp1a2	T	C	9: 57,587,572 (GRCm39)	T333A	probably benign	Het
Cyp2a12	T	C	7: 26,735,944 (GRCm39)	F451S	probably damaging	Het
Dmxl2	T	C	9: 54,316,548 (GRCm39)	I1613V	probably benign	Het
Eme1	A	G	11: 94,540,958 (GRCm39)	L260S	probably damaging	Het
Ermp1	A	C	19: 29,623,415 (GRCm39)	S192A	probably benign	Het
Fam186a	T	C	15: 99,843,079 (GRCm39)	Q1055R	probably benign	Het
Fat1	C	T	8: 45,466,127 (GRCm39)	T1462M	possibly damaging	Het
Fat1	A	G	8: 45,491,336 (GRCm39)	N3822S	probably benign	Het
Gm973	A	G	1: 59,675,317 (GRCm39)	T225A		Het
Gnl2	A	G	4: 124,941,357 (GRCm39)	Y367C	probably damaging	Het
Golga5	T	A	12: 102,458,476 (GRCm39)	S640T	probably benign	Het
Inafm1	A	G	7: 16,007,196 (GRCm39)	V7A	unknown	Het
Irx2	A	G	13: 72,777,392 (GRCm39)	D71G	possibly damaging	Het
Kash5	G	A	7: 44,854,058 (GRCm39)	R24C	unknown	Het
Kif13a	T	C	13: 46,966,263 (GRCm39)	Y448C	probably damaging	Het
Lcor	G	T	19: 41,574,839 (GRCm39)	R1198L	probably damaging	Het
Ldlrad4	A	T	18: 68,197,746 (GRCm39)	N10I	probably benign	Het
Lix1	T	C	17: 17,677,392 (GRCm39)	Y196H	possibly damaging	Het
Lrrc37	A	C	11: 103,509,830 (GRCm39)	S713A	unknown	Het
Mon1a	A	G	9: 107,779,835 (GRCm39)	Y468C	probably damaging	Het
Mroh4	A	T	15: 74,498,140 (GRCm39)	I177N	probably damaging	Het
Mterf4	A	T	1: 93,229,515 (GRCm39)	Y236*	probably null	Het
Myo1f	T	A	17: 33,813,036 (GRCm39)	I614N	probably damaging	Het
Nbas	T	A	12: 13,385,856 (GRCm39)	S707T	possibly damaging	Het
Ncl	A	T	1: 86,279,183 (GRCm39)	S577T	possibly damaging	Het
Or10al4	T	A	17: 38,037,060 (GRCm39)	N48K	probably damaging	Het
Or52d13	T	C	7: 103,109,896 (GRCm39)	D173G		Het
Or6c2b	A	T	10: 128,947,892 (GRCm39)	M134K	probably damaging	Het
Or6c69c	A	T	10: 129,910,941 (GRCm39)	M221L	probably benign	Het
Or6c69c	G	C	10: 129,910,969 (GRCm39)	S230T	probably benign	Het
Or7g17	A	T	9: 18,768,550 (GRCm39)	I201F	probably benign	Het
Or8g17	A	G	9: 38,930,016 (GRCm39)	S274P	probably damaging	Het
Padi1	C	A	4: 140,559,602 (GRCm39)		probably null	Het
Patj	G	A	4: 98,401,871 (GRCm39)	V1004M	probably benign	Het
Pcdhb9	A	T	18: 37,535,770 (GRCm39)	D588V	probably damaging	Het
Pdzph1	C	A	17: 59,261,395 (GRCm39)	R879L	possibly damaging	Het
Pla2g2c	G	A	4: 138,463,378 (GRCm39)	V91I	probably benign	Het
Plin4	T	C	17: 56,416,345 (GRCm39)	D53G	possibly damaging	Het
Poll	T	C	19: 45,546,317 (GRCm39)	Q241R	probably benign	Het
Recql5	A	T	11: 115,785,475 (GRCm39)	L674M	possibly damaging	Het
Shroom3	G	A	5: 93,098,533 (GRCm39)	G1338S	probably damaging	Het
Slc38a8	C	T	8: 120,212,780 (GRCm39)	V294I	probably benign	Het
Slc4a1ap	T	C	5: 31,684,457 (GRCm39)	V31A	probably benign	Het
Tln2	G	A	9: 67,269,927 (GRCm39)	P489S	probably damaging	Het
Trappc9	G	A	15: 72,608,626 (GRCm39)	R928*	probably null	Het
Uba7	C	A	9: 107,855,166 (GRCm39)	T343N	probably benign	Het
Vasn	C	T	16: 4,467,871 (GRCm39)	T606I	probably benign	Het
Xrn2	T	A	2: 146,880,199 (GRCm39)	D507E	probably damaging	Het
Zfp592	T	A	7: 80,674,644 (GRCm39)	M536K	possibly damaging	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4551:Zfp932	UTSW	5	110,157,505 (GRCm39)	missense	probably benign	0.03
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5116:Zfp932	UTSW	5	110,157,242 (GRCm39)	missense	probably benign	0.05
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7437:Zfp932	UTSW	5	110,157,880 (GRCm39)	missense	probably benign	0.10
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9142:Zfp932	UTSW	5	110,157,520 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00
R9640:Zfp932	UTSW	5	110,157,064 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAAGCCTTTGCATTCCATAGTAC -3'
(R):5'- GGCAAAGGATTTAGTACATTGCT -3'

Sequencing Primer
(F):5'- CAGTGTAGTAAAGCCTTTGCAC -3'
(R):5'- AGGCAAAGGATTTAGTACATTGC -3'

Posted On

2021-12-30