Mutagenetix > Incidental Mutation

Incidental Mutation 'R9084:Or1f19'

690493

Institutional Source

Beutler Lab

Gene Symbol

Or1f19

Ensembl Gene

ENSMUSG00000051003

Gene Name

olfactory receptor family 1 subfamily F member 19

Synonyms

GA_x54KRFPKG5P-112942-113883, Olfr161, MOR131-1

MMRRC Submission

068903-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.304) question?

Stock #

R9084 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

3410262-3411203 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 3410617 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Arginine at position 119 (M119R)

Ref Sequence

ENSEMBL: ENSMUSP00000150825 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061541] [ENSMUST00000216259]

AlphaFold

Q8VGB9

Predicted Effect

probably damaging
Transcript: ENSMUST00000061541
AA Change: M119R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000058956
Gene: ENSMUSG00000051003
AA Change: M119R

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	308	6.4e-60	PFAM
Pfam:7TM_GPCR_Srsx	36	220	7.2e-6	PFAM
Pfam:7tm_1	42	291	2.9e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000216259
AA Change: M119R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (62/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930590J08Rik	A	G	6: 91,892,016 (GRCm39)	K93E	probably damaging	Het
Adam21	T	C	12: 81,606,160 (GRCm39)	Y534C	probably damaging	Het
Add1	A	G	5: 34,763,186 (GRCm39)	T125A	probably damaging	Het
Ank3	T	C	10: 69,786,879 (GRCm39)	V981A	probably benign	Het
Ankrd34b	T	A	13: 92,575,720 (GRCm39)	D317E	probably benign	Het
Arhgap9	T	C	10: 127,158,114 (GRCm39)	S44P	possibly damaging	Het
Atg7	T	C	6: 114,678,896 (GRCm39)	S370P	probably damaging	Het
Atp1b2	T	C	11: 69,492,388 (GRCm39)	D224G	probably damaging	Het
C1qtnf6	T	C	15: 78,409,283 (GRCm39)	Y188C	probably damaging	Het
Col22a1	T	C	15: 71,691,929 (GRCm39)	D1297G	unknown	Het
Cpne7	C	T	8: 123,856,951 (GRCm39)	P402L	probably damaging	Het
Csmd1	T	C	8: 16,138,325 (GRCm39)	I1576V	probably benign	Het
Cyp2a12	T	C	7: 26,735,944 (GRCm39)	F451S	probably damaging	Het
Dusp6	T	C	10: 99,099,692 (GRCm39)	S47P	probably benign	Het
Fam163a	T	A	1: 155,955,730 (GRCm39)	I21F	probably damaging	Het
Fer1l5	A	G	1: 36,429,619 (GRCm39)	D457G	probably benign	Het
Fyn	C	T	10: 39,402,845 (GRCm39)	R206C	probably damaging	Het
Git2	G	T	5: 114,902,515 (GRCm39)	H172Q	probably damaging	Het
Gm21698	T	A	5: 26,190,244 (GRCm39)	H151L	probably damaging	Het
Gm4924	T	A	10: 82,213,953 (GRCm39)	C584S	unknown	Het
Hspb8	A	G	5: 116,560,492 (GRCm39)	L16P	probably benign	Het
Ipo9	T	C	1: 135,334,563 (GRCm39)	H282R	probably benign	Het
Kcnn1	GTCCTCCTCCTCCTCCTCCTC	GTCCTCCTCCTCCTCCTC	8: 71,307,810 (GRCm39)		probably benign	Het
Kmt2a	T	C	9: 44,740,130 (GRCm39)	D1871G	unknown	Het
Lrrk2	A	T	15: 91,634,469 (GRCm39)	Q1411L		Het
Med13	A	G	11: 86,191,621 (GRCm39)	S914P	probably damaging	Het
Med15	T	C	16: 17,471,072 (GRCm39)	H706R	probably damaging	Het
Mfsd2a	T	C	4: 122,843,994 (GRCm39)	Y325C	probably damaging	Het
Mrpl9	T	A	3: 94,354,558 (GRCm39)		probably benign	Het
Ncoa2	A	C	1: 13,244,653 (GRCm39)	S682A	probably damaging	Het
Nfe2l1	A	T	11: 96,710,957 (GRCm39)	M424K	probably damaging	Het
Nid1	T	C	13: 13,652,925 (GRCm39)		probably null	Het
Or4f7	A	G	2: 111,644,996 (GRCm39)	L25P	probably damaging	Het
Or4k15b	T	C	14: 50,271,916 (GRCm39)	I315V	probably benign	Het
Or6c69c	A	T	10: 129,910,941 (GRCm39)	M221L	probably benign	Het
Or6c69c	G	C	10: 129,910,969 (GRCm39)	S230T	probably benign	Het
Or6d15	C	T	6: 116,559,232 (GRCm39)	R225H	probably benign	Het
Or8g33	A	T	9: 39,337,521 (GRCm39)	V282E	probably damaging	Het
Pcnt	T	C	10: 76,235,826 (GRCm39)	D1385G	probably benign	Het
Pcx	A	G	19: 4,669,868 (GRCm39)	Y846C	probably damaging	Het
Prune2	A	G	19: 17,097,741 (GRCm39)	M1082V	probably damaging	Het
Ptprm	A	C	17: 67,263,948 (GRCm39)	V433G	possibly damaging	Het
Pvr	G	T	7: 19,650,937 (GRCm39)	Q196K	possibly damaging	Het
Reck	A	G	4: 43,922,809 (GRCm39)		probably benign	Het
Ripk2	T	C	4: 16,123,795 (GRCm39)	D460G	probably damaging	Het
Ryr2	T	C	13: 11,616,724 (GRCm39)	D3898G	probably damaging	Het
Skint8	A	G	4: 111,794,210 (GRCm39)	H200R	probably benign	Het
Slamf1	A	T	1: 171,602,522 (GRCm39)	D83V	probably damaging	Het
Snx25	T	C	8: 46,521,203 (GRCm39)	*143W	probably null	Het
Spata31d1c	A	G	13: 65,182,959 (GRCm39)	D167G	probably benign	Het
Sting1	A	T	18: 35,869,155 (GRCm39)	I178N	probably damaging	Het
Syne1	T	A	10: 5,289,240 (GRCm39)	D1420V	probably benign	Het
Tlcd5	C	T	9: 43,022,664 (GRCm39)	R230Q	probably benign	Het
Tmem116	A	G	5: 121,627,387 (GRCm39)	S211G		Het
Tmprss6	C	T	15: 78,338,417 (GRCm39)	V310M	probably damaging	Het
Ttn	A	G	2: 76,612,722 (GRCm39)	I17119T	probably damaging	Het
Tubb1	T	A	2: 174,299,197 (GRCm39)	M293K	possibly damaging	Het
Unc13d	AATGCCTCCCATGCC	AATGCCTCCCATGCCTCCCATGCC	11: 115,958,998 (GRCm39)		probably benign	Het
Uncx	T	C	5: 139,529,753 (GRCm39)	M2T	possibly damaging	Het
Washc4	T	C	10: 83,422,499 (GRCm39)	F943L	possibly damaging	Het
Xkr9	G	A	1: 13,742,733 (GRCm39)	C6Y	probably benign	Het
Zfp458	A	T	13: 67,407,633 (GRCm39)	V74E	probably benign	Het

Other mutations in Or1f19

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01447:Or1f19	APN	16	3,410,848 (GRCm39)	missense	possibly damaging	0.55
IGL01519:Or1f19	APN	16	3,410,398 (GRCm39)	missense	probably damaging	1.00
IGL02727:Or1f19	APN	16	3,411,190 (GRCm39)	missense	probably benign	0.04
IGL03278:Or1f19	APN	16	3,410,971 (GRCm39)	missense	possibly damaging	0.55
F6893:Or1f19	UTSW	16	3,411,027 (GRCm39)	missense	possibly damaging	0.55
R1634:Or1f19	UTSW	16	3,411,073 (GRCm39)	missense	probably benign	0.19
R2345:Or1f19	UTSW	16	3,411,003 (GRCm39)	missense	probably damaging	0.99
R4858:Or1f19	UTSW	16	3,410,706 (GRCm39)	missense	probably damaging	1.00
R4930:Or1f19	UTSW	16	3,410,299 (GRCm39)	missense	probably damaging	1.00
R6774:Or1f19	UTSW	16	3,410,380 (GRCm39)	missense	probably damaging	1.00
R7480:Or1f19	UTSW	16	3,410,493 (GRCm39)	missense	probably benign	0.03
R7712:Or1f19	UTSW	16	3,410,295 (GRCm39)	missense	probably damaging	1.00
R8685:Or1f19	UTSW	16	3,410,904 (GRCm39)	missense	probably damaging	0.99
R9042:Or1f19	UTSW	16	3,411,132 (GRCm39)	missense	probably damaging	1.00
R9534:Or1f19	UTSW	16	3,410,937 (GRCm39)	missense	probably benign	0.00
R9561:Or1f19	UTSW	16	3,410,725 (GRCm39)	missense	probably damaging	1.00
Z1176:Or1f19	UTSW	16	3,410,997 (GRCm39)	missense	probably benign	0.16
Z1176:Or1f19	UTSW	16	3,410,404 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACCTGCTCATCATCCTGGC -3'
(R):5'- GGATCACATTGTCCCCACAGAAG -3'

Sequencing Primer
(F):5'- TCCTGGCCATCAGCACAG -3'
(R):5'- CATTGTCCCCACAGAAGTAGAGTTG -3'

Posted On

2021-12-30