Mutagenetix > Incidental Mutation

Incidental Mutation 'R9090:Smg1'

690899

Institutional Source

Beutler Lab

Gene Symbol

Smg1

Ensembl Gene

ENSMUSG00000030655

Gene Name

SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Synonyms

2610207I05Rik, 5430435M13Rik, C130002K18Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9090 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

118131308-118243670 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 118212563 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 88 (V88G)

Ref Sequence

ENSEMBL: ENSMUSP00000032891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032891] [ENSMUST00000179047]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000032891
AA Change: V88G

SMART Domains

Protein: ENSMUSP00000032891
Gene: ENSMUSG00000030655
AA Change: V88G

Domain	Start	End	E-Value	Type
low complexity region	42	55	N/A	INTRINSIC
SCOP:d1gw5a_	147	621	7e-7	SMART
Pfam:SMG1	629	1240	9.8e-249	PFAM
low complexity region	1540	1551	N/A	INTRINSIC
SCOP:d1gw5a_	1680	1942	8e-3	SMART
low complexity region	2125	2141	N/A	INTRINSIC
PI3Kc	2149	2493	7.93e-50	SMART
low complexity region	2759	2770	N/A	INTRINSIC
low complexity region	3425	3442	N/A	INTRINSIC
FATC	3626	3658	8.66e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179047
AA Change: V64G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000137172
Gene: ENSMUSG00000030655
AA Change: V64G

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	123	282	7e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179331

SMART Domains

Protein: ENSMUSP00000137592
Gene: ENSMUSG00000030655

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	71	545	1e-6	SMART
low complexity region	602	612	N/A	INTRINSIC
low complexity region	631	646	N/A	INTRINSIC
low complexity region	698	718	N/A	INTRINSIC
low complexity region	898	915	N/A	INTRINSIC
low complexity region	1135	1147	N/A	INTRINSIC
low complexity region	1464	1475	N/A	INTRINSIC
low complexity region	2049	2065	N/A	INTRINSIC
PI3Kc	2073	2417	7.93e-50	SMART
low complexity region	2683	2694	N/A	INTRINSIC
low complexity region	3349	3366	N/A	INTRINSIC
FATC	3550	3582	8.66e-12	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for a gene trap allele exhibit early embryonic lethality. Mice heteroygous for a gene trap allele exhibit abnormal tooth development, chronic inflammation, increased body weight, increased incidence of tumor formation and premature death. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	A	T	11: 120,011,114 (GRCm38)	S819T	probably damaging	Het
Abca13	A	T	11: 9,291,698 (GRCm38)	D1187V	probably damaging	Het
Ak9	C	A	10: 41,424,627 (GRCm38)	T1611K	unknown	Het
Als2	G	T	1: 59,203,030 (GRCm38)	T622K	probably benign	Het
Ankrd40	G	A	11: 94,334,436 (GRCm38)	A98T	probably benign	Het
Ap1b1	T	C	11: 5,023,174 (GRCm38)	L339P	probably damaging	Het
Appl1	A	C	14: 26,947,127 (GRCm38)	H363Q	probably benign	Het
Atf6	T	C	1: 170,794,676 (GRCm38)	N459D	probably damaging	Het
Ccdc6	A	T	10: 70,189,163 (GRCm38)	Q432L	unknown	Het
Ccl21a	T	A	4: 42,773,486 (GRCm38)	Q84L	probably damaging	Het
Cct4	A	G	11: 23,001,389 (GRCm38)	I348V	probably benign	Het
Cd300ld2	A	G	11: 115,013,724 (GRCm38)	Y106H	probably damaging	Het
Cdh19	C	T	1: 110,949,217 (GRCm38)	E131K	probably damaging	Het
Cdon	A	G	9: 35,491,879 (GRCm38)	D1095G	probably damaging	Het
Cep85l	T	A	10: 53,281,574 (GRCm38)	R678*	probably null	Het
Chst3	T	A	10: 60,185,643 (GRCm38)	S461C	probably damaging	Het
Clk1	A	G	1: 58,420,153 (GRCm38)	I149T	possibly damaging	Het
Clvs2	T	A	10: 33,513,305 (GRCm38)	D313V	possibly damaging	Het
Dap3	T	C	3: 88,933,606 (GRCm38)	T75A	probably benign	Het
Dnaaf1	T	A	8: 119,597,653 (GRCm38)	F631L	probably benign	Het
Dnah14	A	T	1: 181,769,760 (GRCm38)	N3549I	probably benign	Het
Dnah17	A	T	11: 118,041,044 (GRCm38)	Y3701N	probably damaging	Het
Dock6	A	G	9: 21,841,500 (GRCm38)	V339A	possibly damaging	Het
Dpp6	T	A	5: 27,598,834 (GRCm38)	C259*	probably null	Het
Dqx1	A	G	6: 83,059,043 (GRCm38)	T119A	probably benign	Het
Dync1h1	G	T	12: 110,616,876 (GRCm38)	R469L	probably benign	Het
Eno4	C	T	19: 58,962,828 (GRCm38)	A424V	probably benign	Het
Entrep1	A	T	19: 23,994,857 (GRCm38)	I161N	possibly damaging	Het
Fads1	T	A	19: 10,185,798 (GRCm38)	D146E	probably damaging	Het
Fam171a1	C	T	2: 3,223,506 (GRCm38)	T303M	probably damaging	Het
Fbxw20	C	T	9: 109,221,355 (GRCm38)	D401N	probably benign	Het
Fcho1	T	A	8: 71,710,424 (GRCm38)	T654S	possibly damaging	Het
Flad1	C	A	3: 89,408,551 (GRCm38)	E235*	probably null	Het
Flrt2	G	T	12: 95,779,133 (GRCm38)	A82S	probably benign	Het
Gabrg3	A	T	7: 57,179,638 (GRCm38)	S124T	probably benign	Het
Galt	C	T	4: 41,756,777 (GRCm38)	T139I	probably benign	Het
Garin4	A	G	1: 191,162,956 (GRCm38)	S497P	probably damaging	Het
Gfod1	T	C	13: 43,303,385 (GRCm38)	E38G	possibly damaging	Het
Gm10330	A	G	12: 23,779,991 (GRCm38)	I63T	possibly damaging	Het
Gm49359	A	G	13: 62,455,053 (GRCm38)	V111A	probably benign	Het
Gpank1	C	T	17: 35,121,758 (GRCm38)		probably benign	Het
Hck	T	C	2: 153,131,265 (GRCm38)	L156P	probably damaging	Het
Hcn3	G	T	3: 89,149,960 (GRCm38)	R444S	probably damaging	Het
Hephl1	T	C	9: 15,076,940 (GRCm38)	N624S	probably damaging	Het
Hmcn1	A	G	1: 150,756,558 (GRCm38)	I876T	probably damaging	Het
Ifi207	CTGTTGATGAAGTTGCATGTGGAGCCAGGAGGTTGCTAGATGTTGTTGATAGAGTTGCATGTGGAGCCAGGAGGTTGCTAGATGTTGTTGATAGAGTTGCATGTGGAGCCAGGAGGTTGCTAGATGCTGTTGATACAGTTGCTTGTGGAGCCAGGAGGTTGCTAGATGCTGTTGATAGAGTTGCATATGGAGCCAGGAGGATGCTATATGTTGTTGATGAAGTTGCATGTGGAGCCAGGAG	CTGTTGATAGAGTTGCATGTGGAGCCAGGAGGTTGCTAGATGTTGTTGATAGAGTTGCATGTGGAGCCAGGAGGTTGCTAGATGCTGTTGATACAGTTGCTTGTGGAGCCAGGAGGTTGCTAGATGCTGTTGATAGAGTTGCATATGGAGCCAGGAGGATGCTATATGTTGTTGATGAAGTTGCATGTGGAGCCAGGAG	1: 173,729,196 (GRCm38)		probably benign	Het
Itga9	T	A	9: 118,671,791 (GRCm38)	D377E	possibly damaging	Het
Itpr3	G	A	17: 27,118,677 (GRCm38)		probably benign	Het
Itprid1	G	T	6: 55,967,066 (GRCm38)	R417L	probably benign	Het
Klk11	C	T	7: 43,776,530 (GRCm38)	L32F	probably benign	Het
Lta4h	A	G	10: 93,474,550 (GRCm38)	S427G	probably benign	Het
Lxn	T	A	3: 67,461,318 (GRCm38)	I122F	probably damaging	Het
Lyz1	A	G	10: 117,288,587 (GRCm38)	V148A	possibly damaging	Het
Mael	G	T	1: 166,204,855 (GRCm38)	Q326K	probably benign	Het
Magi3	T	C	3: 104,015,948 (GRCm38)	D1151G	possibly damaging	Het
Map3k9	C	A	12: 81,722,487 (GRCm38)	G929V	probably benign	Het
Mcoln1	A	G	8: 3,505,771 (GRCm38)	Y22C	probably damaging	Het
Mier3	G	T	13: 111,691,336 (GRCm38)	M45I	probably benign	Het
Mrpl1	T	C	5: 96,223,887 (GRCm38)	V91A	probably damaging	Het
Myo7a	T	C	7: 98,091,074 (GRCm38)	H571R	probably benign	Het
Myom1	T	A	17: 71,067,330 (GRCm38)	W601R	probably damaging	Het
Myom3	C	T	4: 135,778,168 (GRCm38)	T456I	probably benign	Het
Nags	A	T	11: 102,146,758 (GRCm38)	H225L	probably benign	Het
Ncaph	C	A	2: 127,116,634 (GRCm38)	K488N	probably damaging	Het
Nkain3	C	T	4: 20,484,897 (GRCm38)	R60H	probably damaging	Het
Nlrp9a	A	T	7: 26,562,519 (GRCm38)	M698L	probably benign	Het
Nmur2	A	C	11: 56,040,482 (GRCm38)	I134M	probably benign	Het
Nrp2	A	C	1: 62,745,511 (GRCm38)	E273A	probably benign	Het
Nup85	A	G	11: 115,577,961 (GRCm38)	D210G	possibly damaging	Het
Obscn	A	G	11: 59,115,817 (GRCm38)	F1173S	probably damaging	Het
Or5ac24	A	T	16: 59,344,782 (GRCm38)	C306*	probably null	Het
Otogl	T	A	10: 107,817,113 (GRCm38)	E1126V	probably null	Het
Pfkl	T	C	10: 77,997,592 (GRCm38)	I259V	probably benign	Het
Phkg1	A	T	5: 129,865,022 (GRCm38)	Y291N	probably benign	Het
Piezo2	T	G	18: 63,030,379 (GRCm38)	H2156P	probably damaging	Het
Piezo2	C	A	18: 63,075,719 (GRCm38)	V1408L	probably damaging	Het
Pira13	T	A	7: 3,816,998 (GRCm38)	E640V	unknown	Het
Plekhg3	A	G	12: 76,575,920 (GRCm38)	T646A	probably benign	Het
Plk5	C	G	10: 80,357,996 (GRCm38)	R40G	probably damaging	Het
Ppp1r12a	T	C	10: 108,262,363 (GRCm38)	S838P	probably damaging	Het
Rarb	A	T	14: 16,435,235 (GRCm38)	Y270*	probably null	Het
Rbm15	T	C	3: 107,331,996 (GRCm38)	E362G	possibly damaging	Het
Rcan1	A	G	16: 92,465,853 (GRCm38)	F76L		Het
Rdh19	T	C	10: 127,860,273 (GRCm38)	L298P	probably damaging	Het
Rims1	T	A	1: 22,428,522 (GRCm38)	H57L		Het
Rlf	T	C	4: 121,147,554 (GRCm38)	T1520A	probably benign	Het
Rnase1	T	A	14: 51,145,507 (GRCm38)	H130L	possibly damaging	Het
Samd8	T	A	14: 21,792,501 (GRCm38)	M360K	probably damaging	Het
Samhd1	A	G	2: 157,114,285 (GRCm38)	L329P	probably damaging	Het
Scube1	C	T	15: 83,610,193 (GRCm38)	E878K	probably damaging	Het
Sema3b	T	C	9: 107,598,955 (GRCm38)	Y689C	probably damaging	Het
Semp2l2a	T	C	8: 13,836,697 (GRCm38)	T465A	possibly damaging	Het
Sh3pxd2b	A	T	11: 32,423,361 (GRCm38)	N843Y	possibly damaging	Het
Slco1a6	C	T	6: 142,089,849 (GRCm38)	C583Y	probably damaging	Het
Spata24	T	A	18: 35,657,001 (GRCm38)	N146Y	probably damaging	Het
Tcf3	C	A	10: 80,417,357 (GRCm38)	V258L	probably benign	Het
Tiam2	T	C	17: 3,414,736 (GRCm38)	Y247H	probably damaging	Het
Ticrr	T	C	7: 79,660,856 (GRCm38)	F173L	possibly damaging	Het
Tpp2	A	G	1: 43,954,651 (GRCm38)	E232G	probably damaging	Het
Trank1	T	A	9: 111,345,479 (GRCm38)	V138E	probably damaging	Het
Ttll3	T	C	6: 113,392,635 (GRCm38)	S47P	probably benign	Het
Utp4	C	T	8: 106,906,225 (GRCm38)	T280M	probably damaging	Het
Vmn1r123	A	T	7: 21,162,869 (GRCm38)	N229Y	probably benign	Het
Vmn1r85	A	T	7: 13,085,015 (GRCm38)	Y67*	probably null	Het
Zbtb12	T	A	17: 34,895,344 (GRCm38)	V35E	possibly damaging	Het
Zmym6	T	A	4: 127,124,061 (GRCm38)	F1212I	probably damaging	Het

Other mutations in Smg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Smg1	APN	7	118,198,271 (GRCm38)	utr 3 prime	probably benign
IGL00481:Smg1	APN	7	118,210,794 (GRCm38)	missense	possibly damaging	0.67
IGL00503:Smg1	APN	7	118,185,483 (GRCm38)	utr 3 prime	probably benign
IGL00927:Smg1	APN	7	118,140,632 (GRCm38)	missense	probably damaging	1.00
IGL01333:Smg1	APN	7	118,163,378 (GRCm38)	splice site	probably benign
IGL01344:Smg1	APN	7	118,190,836 (GRCm38)	utr 3 prime	probably benign
IGL01397:Smg1	APN	7	118,163,221 (GRCm38)	utr 3 prime	probably benign
IGL01403:Smg1	APN	7	118,158,132 (GRCm38)	utr 3 prime	probably benign
IGL01573:Smg1	APN	7	118,167,962 (GRCm38)	utr 3 prime	probably benign
IGL01872:Smg1	APN	7	118,148,944 (GRCm38)	utr 3 prime	probably benign
IGL02010:Smg1	APN	7	118,186,146 (GRCm38)	utr 3 prime	probably benign
IGL02158:Smg1	APN	7	118,212,946 (GRCm38)	missense	possibly damaging	0.77
IGL02268:Smg1	APN	7	118,182,541 (GRCm38)	missense	probably benign	0.19
IGL02314:Smg1	APN	7	118,154,709 (GRCm38)	utr 3 prime	probably benign
IGL02552:Smg1	APN	7	118,195,894 (GRCm38)	utr 3 prime	probably benign
IGL02577:Smg1	APN	7	118,203,122 (GRCm38)	missense	probably damaging	0.99
IGL02859:Smg1	APN	7	118,148,933 (GRCm38)	utr 3 prime	probably benign
IGL02890:Smg1	APN	7	118,185,501 (GRCm38)	utr 3 prime	probably benign
IGL02892:Smg1	APN	7	118,167,955 (GRCm38)	utr 3 prime	probably benign
IGL03119:Smg1	APN	7	118,195,113 (GRCm38)	utr 3 prime	probably benign
IGL03123:Smg1	APN	7	118,157,181 (GRCm38)	utr 3 prime	probably benign
IGL03128:Smg1	APN	7	118,203,059 (GRCm38)	missense	probably benign	0.03
IGL03184:Smg1	APN	7	118,180,380 (GRCm38)	missense	possibly damaging	0.86
PIT4508001:Smg1	UTSW	7	118,185,541 (GRCm38)	missense	unknown
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0098:Smg1	UTSW	7	118,145,467 (GRCm38)	missense	probably benign	0.02
R0139:Smg1	UTSW	7	118,152,675 (GRCm38)	critical splice donor site	probably null
R0371:Smg1	UTSW	7	118,168,300 (GRCm38)	utr 3 prime	probably benign
R0415:Smg1	UTSW	7	118,182,468 (GRCm38)	missense	probably benign	0.34
R0416:Smg1	UTSW	7	118,184,461 (GRCm38)	splice site	probably benign
R0423:Smg1	UTSW	7	118,176,880 (GRCm38)	missense	possibly damaging	0.53
R0600:Smg1	UTSW	7	118,160,383 (GRCm38)	utr 3 prime	probably benign
R0626:Smg1	UTSW	7	118,182,383 (GRCm38)	missense	possibly damaging	0.82
R0627:Smg1	UTSW	7	118,167,861 (GRCm38)	utr 3 prime	probably benign
R0727:Smg1	UTSW	7	118,166,422 (GRCm38)	utr 3 prime	probably benign
R0729:Smg1	UTSW	7	118,146,289 (GRCm38)	utr 3 prime	probably benign
R0841:Smg1	UTSW	7	118,143,301 (GRCm38)	missense	possibly damaging	0.96
R1114:Smg1	UTSW	7	118,159,790 (GRCm38)	utr 3 prime	probably benign
R1256:Smg1	UTSW	7	118,203,087 (GRCm38)	missense	probably damaging	1.00
R1298:Smg1	UTSW	7	118,168,211 (GRCm38)	utr 3 prime	probably benign
R1370:Smg1	UTSW	7	118,159,752 (GRCm38)	utr 3 prime	probably benign
R1591:Smg1	UTSW	7	118,156,919 (GRCm38)	utr 3 prime	probably benign
R1736:Smg1	UTSW	7	118,165,967 (GRCm38)	splice site	probably null
R1755:Smg1	UTSW	7	118,203,064 (GRCm38)	nonsense	probably null
R1765:Smg1	UTSW	7	118,139,715 (GRCm38)	missense	probably benign	0.03
R1789:Smg1	UTSW	7	118,145,798 (GRCm38)	missense	possibly damaging	0.73
R1845:Smg1	UTSW	7	118,154,622 (GRCm38)	utr 3 prime	probably benign
R1908:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1909:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1942:Smg1	UTSW	7	118,158,103 (GRCm38)	utr 3 prime	probably benign
R2064:Smg1	UTSW	7	118,156,867 (GRCm38)	utr 3 prime	probably benign
R2072:Smg1	UTSW	7	118,163,166 (GRCm38)	utr 3 prime	probably benign
R2154:Smg1	UTSW	7	118,158,076 (GRCm38)	utr 3 prime	probably benign
R2895:Smg1	UTSW	7	118,189,143 (GRCm38)	utr 3 prime	probably benign
R2915:Smg1	UTSW	7	118,210,879 (GRCm38)	splice site	probably benign
R3416:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3417:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3873:Smg1	UTSW	7	118,154,662 (GRCm38)	utr 3 prime	probably benign
R4082:Smg1	UTSW	7	118,160,246 (GRCm38)	utr 3 prime	probably benign
R4230:Smg1	UTSW	7	118,148,733 (GRCm38)	critical splice donor site	probably null
R4304:Smg1	UTSW	7	118,139,518 (GRCm38)	missense	probably benign	0.03
R4549:Smg1	UTSW	7	118,159,683 (GRCm38)	utr 3 prime	probably benign
R4571:Smg1	UTSW	7	118,139,465 (GRCm38)	missense	possibly damaging	0.72
R4638:Smg1	UTSW	7	118,195,926 (GRCm38)	utr 3 prime	probably benign
R4642:Smg1	UTSW	7	118,154,264 (GRCm38)	utr 3 prime	probably benign
R4656:Smg1	UTSW	7	118,212,951 (GRCm38)	missense	probably benign	0.00
R4754:Smg1	UTSW	7	118,156,731 (GRCm38)	utr 3 prime	probably benign
R4798:Smg1	UTSW	7	118,180,474 (GRCm38)	missense	probably benign	0.32
R4906:Smg1	UTSW	7	118,152,408 (GRCm38)	utr 3 prime	probably benign
R4978:Smg1	UTSW	7	118,154,247 (GRCm38)	utr 3 prime	probably benign
R4989:Smg1	UTSW	7	118,208,051 (GRCm38)	missense	probably benign
R4989:Smg1	UTSW	7	118,158,100 (GRCm38)	utr 3 prime	probably benign
R5026:Smg1	UTSW	7	118,193,545 (GRCm38)	utr 3 prime	probably benign
R5124:Smg1	UTSW	7	118,213,012 (GRCm38)	missense	probably benign	0.00
R5318:Smg1	UTSW	7	118,160,204 (GRCm38)	utr 3 prime	probably benign
R5356:Smg1	UTSW	7	118,195,133 (GRCm38)	utr 3 prime	probably benign
R5404:Smg1	UTSW	7	118,206,908 (GRCm38)	missense	probably damaging	1.00
R5423:Smg1	UTSW	7	118,146,071 (GRCm38)	missense	possibly damaging	0.70
R5441:Smg1	UTSW	7	118,195,081 (GRCm38)	utr 3 prime	probably benign
R5490:Smg1	UTSW	7	118,139,436 (GRCm38)	missense	possibly damaging	0.86
R5541:Smg1	UTSW	7	118,157,163 (GRCm38)	utr 3 prime	probably benign
R5564:Smg1	UTSW	7	118,189,819 (GRCm38)	utr 3 prime	probably benign
R5580:Smg1	UTSW	7	118,148,902 (GRCm38)	utr 3 prime	probably benign
R5600:Smg1	UTSW	7	118,167,884 (GRCm38)	utr 3 prime	probably benign
R5628:Smg1	UTSW	7	118,154,701 (GRCm38)	utr 3 prime	probably benign
R5646:Smg1	UTSW	7	118,212,559 (GRCm38)	missense	probably benign	0.42
R5656:Smg1	UTSW	7	118,154,664 (GRCm38)	utr 3 prime	probably benign
R5660:Smg1	UTSW	7	118,143,347 (GRCm38)	missense	probably benign	0.33
R5706:Smg1	UTSW	7	118,145,590 (GRCm38)	missense	possibly damaging	0.86
R5786:Smg1	UTSW	7	118,212,897 (GRCm38)	missense	probably benign	0.12
R5890:Smg1	UTSW	7	118,190,586 (GRCm38)	utr 3 prime	probably benign
R5912:Smg1	UTSW	7	118,154,586 (GRCm38)	utr 3 prime	probably benign
R5977:Smg1	UTSW	7	118,141,357 (GRCm38)	utr 3 prime	probably benign
R5993:Smg1	UTSW	7	118,140,509 (GRCm38)	missense	probably benign	0.33
R6161:Smg1	UTSW	7	118,163,330 (GRCm38)	utr 3 prime	probably benign
R6187:Smg1	UTSW	7	118,189,163 (GRCm38)	utr 3 prime	probably benign
R6264:Smg1	UTSW	7	118,166,087 (GRCm38)	utr 3 prime	probably benign
R6331:Smg1	UTSW	7	118,154,277 (GRCm38)	utr 3 prime	probably benign
R6561:Smg1	UTSW	7	118,166,077 (GRCm38)	utr 3 prime	probably benign
R6571:Smg1	UTSW	7	118,184,514 (GRCm38)	utr 3 prime	probably benign
R6736:Smg1	UTSW	7	118,157,166 (GRCm38)	utr 3 prime	probably benign
R6752:Smg1	UTSW	7	118,163,316 (GRCm38)	utr 3 prime	probably benign
R6777:Smg1	UTSW	7	118,189,117 (GRCm38)	utr 3 prime	probably benign
R6788:Smg1	UTSW	7	118,184,571 (GRCm38)	utr 3 prime	probably benign
R6883:Smg1	UTSW	7	118,168,180 (GRCm38)	utr 3 prime	probably benign
R6991:Smg1	UTSW	7	118,167,868 (GRCm38)	utr 3 prime	probably benign
R7056:Smg1	UTSW	7	118,146,400 (GRCm38)	splice site	probably benign
R7058:Smg1	UTSW	7	118,198,279 (GRCm38)	utr 3 prime	probably benign
R7100:Smg1	UTSW	7	118,184,520 (GRCm38)	missense	unknown
R7133:Smg1	UTSW	7	118,152,908 (GRCm38)	missense	unknown
R7221:Smg1	UTSW	7	118,182,797 (GRCm38)	missense	possibly damaging	0.86
R7229:Smg1	UTSW	7	118,176,955 (GRCm38)	missense	probably benign	0.03
R7293:Smg1	UTSW	7	118,166,099 (GRCm38)	missense	unknown
R7361:Smg1	UTSW	7	118,184,977 (GRCm38)	missense	unknown
R7438:Smg1	UTSW	7	118,195,893 (GRCm38)	missense	unknown
R7686:Smg1	UTSW	7	118,167,858 (GRCm38)	missense	unknown
R7798:Smg1	UTSW	7	118,171,939 (GRCm38)	missense	possibly damaging	0.73
R7908:Smg1	UTSW	7	118,186,134 (GRCm38)	missense	unknown
R7923:Smg1	UTSW	7	118,143,322 (GRCm38)	missense	possibly damaging	0.96
R7978:Smg1	UTSW	7	118,193,655 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,142 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,141 (GRCm38)	missense	unknown
R8025:Smg1	UTSW	7	118,206,989 (GRCm38)	nonsense	probably null
R8056:Smg1	UTSW	7	118,160,366 (GRCm38)	missense	unknown
R8061:Smg1	UTSW	7	118,152,387 (GRCm38)	missense	unknown
R8095:Smg1	UTSW	7	118,173,062 (GRCm38)	missense	unknown
R8198:Smg1	UTSW	7	118,145,606 (GRCm38)	missense	probably benign	0.03
R8399:Smg1	UTSW	7	118,190,571 (GRCm38)	missense	unknown
R8445:Smg1	UTSW	7	118,136,977 (GRCm38)	missense	possibly damaging	0.72
R8519:Smg1	UTSW	7	118,171,759 (GRCm38)	utr 3 prime	probably benign
R8817:Smg1	UTSW	7	118,159,664 (GRCm38)	missense	unknown
R8832:Smg1	UTSW	7	118,139,783 (GRCm38)	missense	probably benign	0.33
R8855:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8866:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8946:Smg1	UTSW	7	118,152,677 (GRCm38)	missense	probably null
R8954:Smg1	UTSW	7	118,206,992 (GRCm38)	missense	probably damaging	1.00
R8967:Smg1	UTSW	7	118,166,516 (GRCm38)	missense	unknown
R9072:Smg1	UTSW	7	118,183,809 (GRCm38)	missense	unknown
R9156:Smg1	UTSW	7	118,154,661 (GRCm38)	missense	unknown
R9198:Smg1	UTSW	7	118,195,956 (GRCm38)	missense	unknown
R9240:Smg1	UTSW	7	118,139,808 (GRCm38)	missense	probably benign	0.18
R9271:Smg1	UTSW	7	118,212,563 (GRCm38)	missense	unknown
R9289:Smg1	UTSW	7	118,145,416 (GRCm38)	missense	possibly damaging	0.53
R9378:Smg1	UTSW	7	118,178,775 (GRCm38)	nonsense	probably null
R9396:Smg1	UTSW	7	118,208,080 (GRCm38)	missense	unknown
R9469:Smg1	UTSW	7	118,140,551 (GRCm38)	missense	possibly damaging	0.72
R9539:Smg1	UTSW	7	118,145,753 (GRCm38)	missense	probably benign	0.03
R9549:Smg1	UTSW	7	118,196,031 (GRCm38)	missense	unknown
R9563:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9564:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9597:Smg1	UTSW	7	118,213,047 (GRCm38)	missense	unknown
R9643:Smg1	UTSW	7	118,156,710 (GRCm38)	missense	unknown
R9703:Smg1	UTSW	7	118,140,521 (GRCm38)	missense	possibly damaging	0.73
R9730:Smg1	UTSW	7	118,183,781 (GRCm38)	missense	unknown
Z1088:Smg1	UTSW	7	118,178,399 (GRCm38)	missense	possibly damaging	0.96
Z1088:Smg1	UTSW	7	118,168,661 (GRCm38)	nonsense	probably null
Z1088:Smg1	UTSW	7	118,154,635 (GRCm38)	utr 3 prime	probably benign
Z1176:Smg1	UTSW	7	118,206,907 (GRCm38)	missense	unknown
Z1176:Smg1	UTSW	7	118,206,887 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,213,033 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,168,608 (GRCm38)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- TCAACCCTGGTCTCCAAGTC -3'
(R):5'- GAGGTTCTTCCAGGCTACTGTC -3'

Sequencing Primer
(F):5'- GGTCTCCAAGTCTTACCCTGTG -3'
(R):5'- GCTTAAAGCATCTGTCTGAATTGAAC -3'

Posted On

2021-12-30