Mutagenetix > Incidental Mutation

Incidental Mutation 'R9092:Zfp608'

691100

Institutional Source

Beutler Lab

Gene Symbol

Zfp608

Ensembl Gene

ENSMUSG00000052713

Gene Name

zinc finger protein 608

Synonyms

4932417D18Rik

MMRRC Submission

068908-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.899) question?

Stock #

R9092 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

55021120-55125627 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 55031648 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 764 (I764T)

Ref Sequence

ENSEMBL: ENSMUSP00000068192 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064763]

AlphaFold

Q56A10

Predicted Effect

probably benign
Transcript: ENSMUST00000064763
AA Change: I764T

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000068192
Gene: ENSMUSG00000052713
AA Change: I764T

Domain	Start	End	E-Value	Type
low complexity region	51	61	N/A	INTRINSIC
low complexity region	150	184	N/A	INTRINSIC
low complexity region	214	234	N/A	INTRINSIC
low complexity region	252	266	N/A	INTRINSIC
low complexity region	309	326	N/A	INTRINSIC
low complexity region	428	445	N/A	INTRINSIC
low complexity region	459	471	N/A	INTRINSIC
ZnF_C2H2	552	577	1.86e0	SMART
low complexity region	624	636	N/A	INTRINSIC
low complexity region	706	718	N/A	INTRINSIC
low complexity region	734	773	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	833	846	N/A	INTRINSIC
low complexity region	934	956	N/A	INTRINSIC
coiled coil region	1028	1054	N/A	INTRINSIC
low complexity region	1182	1193	N/A	INTRINSIC
low complexity region	1255	1268	N/A	INTRINSIC
low complexity region	1439	1450	N/A	INTRINSIC
low complexity region	1458	1466	N/A	INTRINSIC
low complexity region	1486	1502	N/A	INTRINSIC

Meta Mutation Damage Score

0.0611

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.8%
20x: 99.4%

Validation Efficiency

93% (56/60)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Chl1	T	C	6: 103,645,815 (GRCm39)		probably benign	Het
Crtc3	T	C	7: 80,239,628 (GRCm39)	M575V	probably benign	Het
Cux1	T	A	5: 136,514,671 (GRCm39)	N22I	probably damaging	Het
Dcun1d2	G	A	8: 13,307,935 (GRCm39)	R248W	probably damaging	Het
Dlc1	G	T	8: 37,199,860 (GRCm39)	H7Q	probably benign	Het
Drd2	A	G	9: 49,307,004 (GRCm39)	D30G	probably benign	Het
Duxf3	GCCC	GCC	10: 58,066,944 (GRCm39)		probably null	Het
E2f7	T	C	10: 110,616,874 (GRCm39)	S705P	probably benign	Het
Ephb3	G	A	16: 21,041,214 (GRCm39)	S977N	probably benign	Het
F13a1	T	G	13: 37,089,993 (GRCm39)	D448A	probably benign	Het
Fam216b	T	C	14: 78,322,537 (GRCm39)	T56A	possibly damaging	Het
Fcho2	T	C	13: 98,886,391 (GRCm39)	T408A	probably benign	Het
Flvcr1	A	T	1: 190,740,364 (GRCm39)	V552E		Het
Gab3	CTT	CTTGTT	X: 74,043,612 (GRCm39)		probably benign	Het
Gab3	TCT	TCTGCT	X: 74,043,602 (GRCm39)		probably benign	Het
Galnt15	A	G	14: 31,780,196 (GRCm39)	K622E	probably benign	Het
Gjb3	GCCAGATGCGCCCA	GCCAGATGCGCCCAGATGCGCCCA	4: 127,220,458 (GRCm39)		probably null	Het
Gjb3	A	AGATGCGCCCG	4: 127,220,471 (GRCm39)		probably null	Het
Gm5414	T	G	15: 101,536,345 (GRCm39)	R93S	probably benign	Het
Gtf2i	A	G	5: 134,318,241 (GRCm39)	*87Q	probably null	Het
Il7r	T	A	15: 9,510,270 (GRCm39)	H261L	probably benign	Het
Itsn1	T	A	16: 91,609,002 (GRCm39)	M250K	possibly damaging	Het
Krt9	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	TCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCCCCCACTTCCTCCTCCATAGCTGCC	11: 100,079,903 (GRCm39)		probably benign	Het
Liat1	A	G	11: 75,893,887 (GRCm39)	D88G	possibly damaging	Het
Lrrc47	C	A	4: 154,096,421 (GRCm39)	T72K	possibly damaging	Het
Map2k4	C	A	11: 65,581,599 (GRCm39)	R371L	probably benign	Het
Mmrn2	T	C	14: 34,118,587 (GRCm39)	F158L	probably benign	Het
Mtus1	A	T	8: 41,455,475 (GRCm39)	L242Q	probably damaging	Het
Myo5a	T	C	9: 75,054,414 (GRCm39)		probably null	Het
Noc3l	T	A	19: 38,798,487 (GRCm39)	K305I	probably damaging	Het
Or4p8	T	C	2: 88,727,321 (GRCm39)	T207A	probably damaging	Het
Pag1	T	C	3: 9,764,848 (GRCm39)	T102A	probably benign	Het
Pam	A	G	1: 97,791,976 (GRCm39)	S482P	probably benign	Het
Pax1	T	G	2: 147,204,287 (GRCm39)	W23G	unknown	Het
Pcdhgb1	T	A	18: 37,813,989 (GRCm39)	V160D	possibly damaging	Het
Pdzph1	T	C	17: 59,280,125 (GRCm39)	D719G	probably damaging	Het
Phlda1	T	C	10: 111,342,474 (GRCm39)	L70S	possibly damaging	Het
Pikfyve	G	A	1: 65,283,559 (GRCm39)	R732K	probably damaging	Het
Pkd1	G	T	17: 24,788,347 (GRCm39)	V702F	possibly damaging	Het
Pkhd1	A	G	1: 20,632,586 (GRCm39)	Y610H	probably benign	Het
Pofut1	T	G	2: 153,101,508 (GRCm39)	H87Q	probably benign	Het
Runx2	C	A	17: 45,046,443 (GRCm39)	D109Y	probably damaging	Het
Serpina1b	T	A	12: 103,696,540 (GRCm39)	I290F	probably benign	Het
Sez6	G	A	11: 77,865,121 (GRCm39)	E623K	possibly damaging	Het
Sh3tc1	T	C	5: 35,874,321 (GRCm39)	N198S	probably benign	Het
Slc27a6	C	A	18: 58,742,330 (GRCm39)	R515S	probably benign	Het
Sorbs3	C	T	14: 70,445,004 (GRCm39)	V25I	probably benign	Het
Speg	A	G	1: 75,399,378 (GRCm39)	E2275G	probably benign	Het
Tmem161b	C	T	13: 84,440,503 (GRCm39)	T307I	possibly damaging	Het
Tmem30a	T	C	9: 79,678,581 (GRCm39)	D361G	probably damaging	Het
Tmprss11c	T	C	5: 86,385,495 (GRCm39)	T326A	probably benign	Het
Tpbg	T	C	9: 85,726,916 (GRCm39)	V295A	possibly damaging	Het
Ulk4	A	T	9: 120,903,003 (GRCm39)	I1158N		Het
Utp20	C	A	10: 88,604,679 (GRCm39)	A1739S	probably benign	Het
Utp20	T	C	10: 88,611,180 (GRCm39)	N1379S	probably damaging	Het
Vmn1r66	T	A	7: 10,008,110 (GRCm39)	I308F	possibly damaging	Het
Vmn2r101	A	T	17: 19,809,807 (GRCm39)	T198S	probably benign	Het
Vps16	T	C	2: 130,281,593 (GRCm39)	I318T	probably damaging	Het
Wdr11	T	A	7: 129,226,451 (GRCm39)	W750R	probably damaging	Het
Zfp708	C	A	13: 67,218,564 (GRCm39)	D420Y	probably damaging	Het

Other mutations in Zfp608

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00500:Zfp608	APN	18	55,121,405 (GRCm39)	missense	probably benign
IGL00920:Zfp608	APN	18	55,022,903 (GRCm39)	missense	probably benign	0.00
IGL01088:Zfp608	APN	18	55,031,159 (GRCm39)	missense	probably benign	0.03
IGL01447:Zfp608	APN	18	55,032,083 (GRCm39)	missense	possibly damaging	0.75
IGL01534:Zfp608	APN	18	55,032,004 (GRCm39)	missense	probably damaging	0.99
IGL01547:Zfp608	APN	18	55,027,521 (GRCm39)	critical splice donor site	probably null
IGL01933:Zfp608	APN	18	55,120,871 (GRCm39)	missense	probably benign
IGL01998:Zfp608	APN	18	55,024,890 (GRCm39)	missense	probably damaging	0.99
IGL02167:Zfp608	APN	18	55,121,296 (GRCm39)	missense	probably damaging	1.00
IGL02266:Zfp608	APN	18	55,030,653 (GRCm39)	missense	probably benign	0.08
IGL02335:Zfp608	APN	18	55,030,509 (GRCm39)	nonsense	probably null
IGL02455:Zfp608	APN	18	55,032,405 (GRCm39)	missense	probably damaging	1.00
IGL02612:Zfp608	APN	18	55,031,273 (GRCm39)	missense	probably damaging	1.00
IGL02900:Zfp608	APN	18	55,079,865 (GRCm39)	missense	probably damaging	1.00
PIT4243001:Zfp608	UTSW	18	55,031,096 (GRCm39)	missense	probably damaging	1.00
PIT4519001:Zfp608	UTSW	18	55,079,783 (GRCm39)	missense	possibly damaging	0.71
R0005:Zfp608	UTSW	18	55,028,592 (GRCm39)	missense	possibly damaging	0.88
R0010:Zfp608	UTSW	18	55,028,286 (GRCm39)	splice site	probably benign
R0010:Zfp608	UTSW	18	55,028,286 (GRCm39)	splice site	probably benign
R0064:Zfp608	UTSW	18	55,031,888 (GRCm39)	missense	probably benign
R0401:Zfp608	UTSW	18	55,032,066 (GRCm39)	missense	probably benign
R0722:Zfp608	UTSW	18	55,033,306 (GRCm39)	missense	probably damaging	1.00
R1351:Zfp608	UTSW	18	55,031,463 (GRCm39)	missense	probably benign	0.00
R1512:Zfp608	UTSW	18	55,079,738 (GRCm39)	missense	probably damaging	1.00
R1554:Zfp608	UTSW	18	55,031,126 (GRCm39)	missense	probably damaging	1.00
R1622:Zfp608	UTSW	18	55,121,366 (GRCm39)	missense	probably benign	0.07
R1669:Zfp608	UTSW	18	55,120,811 (GRCm39)	missense	probably benign	0.09
R1690:Zfp608	UTSW	18	55,120,706 (GRCm39)	missense	possibly damaging	0.62
R1721:Zfp608	UTSW	18	55,032,321 (GRCm39)	missense	probably benign
R1826:Zfp608	UTSW	18	55,031,648 (GRCm39)	missense	probably benign	0.03
R1864:Zfp608	UTSW	18	55,030,983 (GRCm39)	missense	probably benign	0.00
R1952:Zfp608	UTSW	18	55,030,851 (GRCm39)	nonsense	probably null
R2049:Zfp608	UTSW	18	55,028,637 (GRCm39)	missense	probably damaging	1.00
R2051:Zfp608	UTSW	18	55,121,386 (GRCm39)	missense	probably benign
R2168:Zfp608	UTSW	18	55,031,125 (GRCm39)	nonsense	probably null
R2218:Zfp608	UTSW	18	55,120,756 (GRCm39)	missense	probably benign	0.14
R2283:Zfp608	UTSW	18	55,121,446 (GRCm39)	missense	probably damaging	1.00
R2399:Zfp608	UTSW	18	55,030,974 (GRCm39)	missense	probably damaging	1.00
R2520:Zfp608	UTSW	18	55,121,578 (GRCm39)	missense	possibly damaging	0.92
R2961:Zfp608	UTSW	18	55,031,544 (GRCm39)	missense	possibly damaging	0.60
R4074:Zfp608	UTSW	18	55,031,180 (GRCm39)	missense	probably damaging	1.00
R4076:Zfp608	UTSW	18	55,031,180 (GRCm39)	missense	probably damaging	1.00
R4206:Zfp608	UTSW	18	55,121,267 (GRCm39)	missense	probably damaging	1.00
R4756:Zfp608	UTSW	18	55,027,544 (GRCm39)	missense	probably damaging	1.00
R4771:Zfp608	UTSW	18	55,121,372 (GRCm39)	missense	probably benign
R4820:Zfp608	UTSW	18	55,120,756 (GRCm39)	missense	probably benign	0.14
R4825:Zfp608	UTSW	18	55,031,041 (GRCm39)	missense	probably benign	0.01
R4912:Zfp608	UTSW	18	55,079,663 (GRCm39)	missense	probably damaging	1.00
R4975:Zfp608	UTSW	18	55,022,962 (GRCm39)	missense	probably damaging	1.00
R5138:Zfp608	UTSW	18	55,024,871 (GRCm39)	missense	probably damaging	1.00
R5192:Zfp608	UTSW	18	55,031,569 (GRCm39)	missense	probably damaging	1.00
R5557:Zfp608	UTSW	18	55,120,942 (GRCm39)	missense	possibly damaging	0.81
R5624:Zfp608	UTSW	18	55,031,344 (GRCm39)	missense	probably damaging	1.00
R5818:Zfp608	UTSW	18	55,028,468 (GRCm39)	missense	probably benign	0.02
R5840:Zfp608	UTSW	18	55,031,978 (GRCm39)	missense	probably damaging	1.00
R5992:Zfp608	UTSW	18	55,032,320 (GRCm39)	missense	probably benign	0.15
R6106:Zfp608	UTSW	18	55,120,944 (GRCm39)	missense	possibly damaging	0.54
R6174:Zfp608	UTSW	18	55,121,616 (GRCm39)	start gained	probably benign
R6181:Zfp608	UTSW	18	55,028,700 (GRCm39)	missense	possibly damaging	0.56
R6517:Zfp608	UTSW	18	55,032,150 (GRCm39)	missense	possibly damaging	0.94
R6567:Zfp608	UTSW	18	55,030,628 (GRCm39)	missense	probably damaging	1.00
R6668:Zfp608	UTSW	18	55,031,091 (GRCm39)	missense	probably damaging	1.00
R6920:Zfp608	UTSW	18	55,121,337 (GRCm39)	missense	probably damaging	1.00
R7061:Zfp608	UTSW	18	55,121,069 (GRCm39)	missense	probably benign
R7074:Zfp608	UTSW	18	55,030,454 (GRCm39)	missense	possibly damaging	0.69
R7087:Zfp608	UTSW	18	55,032,469 (GRCm39)	missense	probably damaging	1.00
R7391:Zfp608	UTSW	18	55,030,619 (GRCm39)	missense	possibly damaging	0.49
R7600:Zfp608	UTSW	18	55,121,092 (GRCm39)	missense	probably damaging	1.00
R7723:Zfp608	UTSW	18	55,030,673 (GRCm39)	missense	probably damaging	1.00
R8054:Zfp608	UTSW	18	55,032,618 (GRCm39)	missense	probably benign	0.28
R8236:Zfp608	UTSW	18	55,032,281 (GRCm39)	missense	probably damaging	1.00
R8260:Zfp608	UTSW	18	55,030,821 (GRCm39)	missense	possibly damaging	0.60
R8732:Zfp608	UTSW	18	55,121,072 (GRCm39)	missense	probably benign	0.06
R8781:Zfp608	UTSW	18	55,031,801 (GRCm39)	missense	probably damaging	1.00
R8851:Zfp608	UTSW	18	55,032,194 (GRCm39)	missense	possibly damaging	0.94
R8940:Zfp608	UTSW	18	55,033,301 (GRCm39)	missense	possibly damaging	0.93
R9051:Zfp608	UTSW	18	55,032,266 (GRCm39)	missense	probably damaging	1.00
R9091:Zfp608	UTSW	18	55,032,190 (GRCm39)	missense	probably damaging	1.00
R9236:Zfp608	UTSW	18	55,032,354 (GRCm39)	missense	probably benign	0.39
R9270:Zfp608	UTSW	18	55,032,190 (GRCm39)	missense	probably damaging	1.00
R9283:Zfp608	UTSW	18	55,030,913 (GRCm39)	missense	possibly damaging	0.50
R9288:Zfp608	UTSW	18	55,033,341 (GRCm39)	missense	probably benign	0.39
R9463:Zfp608	UTSW	18	55,030,274 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAGCATCCATTTTGGGACCTC -3'
(R):5'- AATGCCCAAATTGGAAGCGG -3'

Sequencing Primer
(F):5'- GGACCTCCAGTCTCTTTCCC -3'
(R):5'- CTGATTGACAAGAAAAGTTTGGGG -3'

Posted On

2021-12-30