Mutagenetix > Incidental Mutation

Incidental Mutation 'R9098:Or1o2'

691514

Institutional Source

Beutler Lab

Gene Symbol

Or1o2

Ensembl Gene

ENSMUSG00000058802

Gene Name

olfactory receptor family 1 subfamily O member 2

Synonyms

MOR156-2, GA_x6K02T2PSCP-1672287-1671355, Olfr97

MMRRC Submission

068913-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9098 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37542327-37543259 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 37542961 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 100 (Y100C)

Ref Sequence

ENSEMBL: ENSMUSP00000094937 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073667] [ENSMUST00000207414] [ENSMUST00000213328] [ENSMUST00000214622] [ENSMUST00000214848] [ENSMUST00000216225]

AlphaFold

Q8VFE1

Predicted Effect

probably benign
Transcript: ENSMUST00000073667
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000094937
Gene: ENSMUSG00000058802
AA Change: Y100C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srv	24	305	4.5e-9	PFAM
Pfam:7tm_4	29	306	1.4e-54	PFAM
Pfam:7TM_GPCR_Srsx	33	303	1.1e-5	PFAM
Pfam:7tm_1	39	288	8.6e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000207414
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000213328
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000214622
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000214848
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000216225
AA Change: Y100C

PolyPhen 2 Score 0.115 (Sensitivity: 0.93; Specificity: 0.86)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

97% (72/74)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	A	T	1: 11,633,211 (GRCm39)	D168V	probably damaging	Het
Abcb4	A	T	5: 9,008,441 (GRCm39)	D1203V	probably damaging	Het
Acat2	G	A	17: 13,178,979 (GRCm39)		probably benign	Het
Adam3	C	T	8: 25,179,484 (GRCm39)	C611Y	probably damaging	Het
Adgrb1	A	G	15: 74,415,189 (GRCm39)	D638G	probably damaging	Het
Adra1b	T	C	11: 43,667,218 (GRCm39)	N340D	probably damaging	Het
Ankrd31	A	G	13: 96,916,879 (GRCm39)	H131R	probably damaging	Het
Aoc2	T	C	11: 101,217,164 (GRCm39)	S416P	possibly damaging	Het
Armc8	G	A	9: 99,387,362 (GRCm39)	R419*	probably null	Het
Atp6v1h	T	G	1: 5,163,638 (GRCm39)	V90G	probably damaging	Het
Avl9	T	C	6: 56,707,628 (GRCm39)	V191A	probably benign	Het
Bsn	G	A	9: 107,990,173 (GRCm39)	P1860S	possibly damaging	Het
Btd	A	G	14: 31,384,233 (GRCm39)	K73R	probably benign	Het
Cd22	T	C	7: 30,567,391 (GRCm39)	K731R	probably benign	Het
Chmp7	C	A	14: 69,956,911 (GRCm39)	L332F	probably damaging	Het
Cirop	T	G	14: 54,932,686 (GRCm39)	S393R	probably damaging	Het
Cldn11	A	G	3: 31,217,276 (GRCm39)	E148G	probably damaging	Het
Cmtm1	TCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGG	TCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGGCTGGCTGGTGTCCCGGGTACTGAAGGTCCCTGG	8: 105,036,334 (GRCm39)		probably null	Het
Cnbd1	A	T	4: 18,886,061 (GRCm39)	Y319*	probably null	Het
Cnnm4	A	G	1: 36,511,170 (GRCm39)	T133A	probably benign	Het
Cpeb4	A	T	11: 31,822,679 (GRCm39)	E131V	probably benign	Het
Ctns	A	G	11: 73,078,561 (GRCm39)		probably null	Het
Dhx36	C	A	3: 62,414,142 (GRCm39)	Q73H	probably benign	Het
Dhx36	C	A	3: 62,414,141 (GRCm39)	E74*	probably null	Het
Dnah5	A	G	15: 28,420,107 (GRCm39)	T3802A		Het
Fbxo31	G	A	8: 122,281,136 (GRCm39)	R337C	probably damaging	Het
Flnc	C	T	6: 29,455,518 (GRCm39)	Q2056*	probably null	Het
Fmo9	T	C	1: 166,492,199 (GRCm39)	D408G	possibly damaging	Het
Gipr	C	T	7: 18,897,495 (GRCm39)	S117N	unknown	Het
Gm14412	T	A	2: 177,006,356 (GRCm39)	H513L	probably damaging	Het
Gm21663	G	C	5: 26,147,167 (GRCm39)	C46W	probably benign	Het
Gucy1a2	A	T	9: 3,634,489 (GRCm39)	I178L	probably benign	Het
Htr1b	A	T	9: 81,514,481 (GRCm39)	L42Q		Het
Htr2b	T	C	1: 86,027,481 (GRCm39)	T342A	probably damaging	Het
Jkampl	T	C	6: 73,446,517 (GRCm39)	T11A	probably benign	Het
Jph2	C	T	2: 163,181,473 (GRCm39)	V564M	probably damaging	Het
Kcnmb1	A	G	11: 33,914,806 (GRCm39)	T36A	probably damaging	Het
Krit1	A	C	5: 3,863,135 (GRCm39)	K310N	probably benign	Het
Lama1	C	T	17: 68,111,508 (GRCm39)	T2253M		Het
Lpar6	C	T	14: 73,476,233 (GRCm39)	L65F	probably damaging	Het
Megf6	G	A	4: 154,354,160 (GRCm39)	G1355R	probably damaging	Het
Mmp3	T	A	9: 7,446,936 (GRCm39)	Y39N	probably damaging	Het
Mrtfb	A	G	16: 13,221,053 (GRCm39)	H743R	probably benign	Het
Ms4a18	C	A	19: 10,990,741 (GRCm39)	V118F		Het
Mthfr	G	A	4: 148,126,082 (GRCm39)	S51N	probably benign	Het
Muc16	T	A	9: 18,554,975 (GRCm39)	T3773S	unknown	Het
Mus81	T	A	19: 5,534,032 (GRCm39)	K400*	probably null	Het
Naaladl2	A	T	3: 24,487,344 (GRCm39)	D192E	probably benign	Het
Naip5	T	C	13: 100,366,127 (GRCm39)	D329G	possibly damaging	Het
Nek11	T	A	9: 105,170,856 (GRCm39)	H393L	probably benign	Het
Nmt2	T	A	2: 3,306,315 (GRCm39)		probably benign	Het
Nmur2	T	C	11: 55,920,408 (GRCm39)	D279G	possibly damaging	Het
Nol4l	C	A	2: 153,312,630 (GRCm39)	R226L	probably damaging	Het
Ogfr	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	GGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGAGCCAGAGGACCCCAAAAGCCAGGTGGGGCCAGAGGACCCCCAAAGCCAGGTGG	2: 180,237,059 (GRCm39)		probably benign	Het
Or10d4c	A	G	9: 39,558,379 (GRCm39)	D119G	probably damaging	Het
Or5ak22	T	C	2: 85,229,995 (GRCm39)	N294S	probably damaging	Het
Palm	A	G	10: 79,654,988 (GRCm39)	T249A	probably benign	Het
Pramel22	A	T	4: 143,381,097 (GRCm39)	Y309N	probably benign	Het
Rhobtb3	A	G	13: 76,087,702 (GRCm39)	I95T	probably damaging	Het
Rnpep	A	T	1: 135,206,559 (GRCm39)	F178L	possibly damaging	Het
Rsl1d1	A	G	16: 11,019,227 (GRCm39)	S105P	probably damaging	Het
Rxra	C	T	2: 27,638,756 (GRCm39)	T253I	possibly damaging	Het
Schip1	A	G	3: 67,972,318 (GRCm39)	D15G		Het
Sis	T	A	3: 72,844,578 (GRCm39)	I719F	possibly damaging	Het
Snx18	A	G	13: 113,754,310 (GRCm39)	S208P	probably benign	Het
Srcap	T	C	7: 127,151,816 (GRCm39)	V2125A	probably damaging	Het
Stx7	C	T	10: 24,058,724 (GRCm39)	Q199*	probably null	Het
Syt6	T	C	3: 103,492,895 (GRCm39)	I134T	probably damaging	Het
Tlr12	A	G	4: 128,510,870 (GRCm39)	M460T	probably benign	Het
Tnni3k	T	C	3: 154,647,314 (GRCm39)	T398A	possibly damaging	Het
Tspan4	T	C	7: 141,071,816 (GRCm39)	S188P	probably benign	Het
Ubap2l	A	G	3: 89,909,756 (GRCm39)	S1025P	unknown	Het
Ubl7	A	G	9: 57,829,035 (GRCm39)	D219G	probably benign	Het
Vmn2r115	C	A	17: 23,564,803 (GRCm39)	T230K	probably benign	Het
Vmn2r95	T	A	17: 18,660,167 (GRCm39)	M193K	possibly damaging	Het
Zfp639	A	G	3: 32,573,885 (GRCm39)	E170G	probably damaging	Het
Zfy1	A	G	Y: 725,987 (GRCm39)	S593P	possibly damaging	Het
Zscan4-ps1	T	A	7: 10,799,495 (GRCm39)	T465S	probably damaging	Het

Other mutations in Or1o2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01934:Or1o2	APN	17	37,542,439 (GRCm39)	missense	probably damaging	1.00
IGL02424:Or1o2	APN	17	37,543,263 (GRCm39)	start gained	probably benign
IGL03129:Or1o2	APN	17	37,543,087 (GRCm39)	missense	probably damaging	1.00
R0619:Or1o2	UTSW	17	37,543,046 (GRCm39)	missense	possibly damaging	0.92
R0811:Or1o2	UTSW	17	37,543,223 (GRCm39)	missense	probably benign	0.00
R0812:Or1o2	UTSW	17	37,543,223 (GRCm39)	missense	probably benign	0.00
R1122:Or1o2	UTSW	17	37,542,934 (GRCm39)	missense	probably damaging	1.00
R1927:Or1o2	UTSW	17	37,542,434 (GRCm39)	missense	probably damaging	1.00
R1997:Or1o2	UTSW	17	37,542,523 (GRCm39)	missense	probably damaging	1.00
R3771:Or1o2	UTSW	17	37,542,356 (GRCm39)	missense	possibly damaging	0.53
R3775:Or1o2	UTSW	17	37,543,121 (GRCm39)	missense	probably damaging	0.98
R4182:Or1o2	UTSW	17	37,542,739 (GRCm39)	missense	possibly damaging	0.93
R4183:Or1o2	UTSW	17	37,542,739 (GRCm39)	missense	possibly damaging	0.93
R4184:Or1o2	UTSW	17	37,542,739 (GRCm39)	missense	possibly damaging	0.93
R4198:Or1o2	UTSW	17	37,543,025 (GRCm39)	missense	probably benign	0.01
R7453:Or1o2	UTSW	17	37,542,871 (GRCm39)	missense	probably damaging	1.00
R7615:Or1o2	UTSW	17	37,542,341 (GRCm39)	missense	probably benign	0.25
R7829:Or1o2	UTSW	17	37,543,201 (GRCm39)	missense	probably benign	0.01
R8223:Or1o2	UTSW	17	37,542,727 (GRCm39)	missense	possibly damaging	0.89
R8526:Or1o2	UTSW	17	37,542,470 (GRCm39)	missense	probably damaging	0.97
Z1177:Or1o2	UTSW	17	37,543,187 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGGGTCAGAGACACACAATG -3'
(R):5'- GAAATGGCAACCCCTCTTTAGC -3'

Sequencing Primer
(F):5'- GGTCAGAGACACACAATGAGCTACTC -3'
(R):5'- GGAACCTGCTACTTTTGTTAGC -3'

Posted On

2021-12-30