Mutagenetix > Incidental Mutation

Incidental Mutation 'R9103:Fstl4'

691830

Institutional Source

Beutler Lab

Gene Symbol

Fstl4

Ensembl Gene

ENSMUSG00000036264

Gene Name

follistatin-like 4

Synonyms

SPIG1, B230374F23Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.151) question?

Stock #

R9103 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

52655461-53079365 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 52664696 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Asparagine at position 9 (H9N)

Ref Sequence

ENSEMBL: ENSMUSP00000042007 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036796]

AlphaFold

Q5STE3

Predicted Effect

probably benign
Transcript: ENSMUST00000036796
AA Change: H9N

PolyPhen 2 Score 0.210 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000042007
Gene: ENSMUSG00000036264
AA Change: H9N

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
KAZAL	87	132	4.7e-13	SMART
Blast:IG_like	215	241	6e-7	BLAST
IGc2	260	327	1.9e-6	SMART
IGc2	352	419	1e-14	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

100% (73/73)

MGI Phenotype

PHENOTYPE: Homozygous null mice were born at expected Mendelian ratio and healthy, fertile, apparently normal with normal retinal laminar structure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam6b	T	A	12: 113,454,558 (GRCm39)	C458*	probably null	Het
Adgra2	G	A	8: 27,603,436 (GRCm39)	G407D	probably damaging	Het
Aox4	G	A	1: 58,296,441 (GRCm39)	G1046E	probably damaging	Het
Arhgap27	A	G	11: 103,251,540 (GRCm39)	Y63H	probably damaging	Het
Ascl3	A	T	7: 109,327,127 (GRCm39)	M64K	probably benign	Het
Cacna2d3	G	A	14: 29,068,971 (GRCm39)	T252I	probably damaging	Het
Capn7	A	G	14: 31,091,732 (GRCm39)	T710A	probably benign	Het
Carmil1	A	G	13: 24,295,836 (GRCm39)	C393R	probably benign	Het
Chd5	A	C	4: 152,461,444 (GRCm39)	E1215A	possibly damaging	Het
Cntnap1	A	G	11: 101,072,094 (GRCm39)	N449S	probably benign	Het
Cpz	G	A	5: 35,675,054 (GRCm39)	S65L	probably benign	Het
Cyp2c68	T	A	19: 39,727,625 (GRCm39)	N118I	possibly damaging	Het
Ddx25	A	T	9: 35,458,085 (GRCm39)	F345I	probably benign	Het
Dennd2d	T	C	3: 106,397,684 (GRCm39)	Y161H		Het
Egfl8	C	T	17: 34,833,210 (GRCm39)	D179N	probably benign	Het
Eif2a	A	G	3: 58,452,461 (GRCm39)	N174S		Het
Farp1	C	T	14: 121,509,104 (GRCm39)	H643Y	possibly damaging	Het
Fat1	G	T	8: 45,404,850 (GRCm39)	V534L	probably benign	Het
Fbxo48	A	G	11: 16,903,556 (GRCm39)	T61A	probably benign	Het
Fnbp4	A	G	2: 90,608,187 (GRCm39)	T970A	probably benign	Het
Foxd1	T	A	13: 98,491,763 (GRCm39)	D212E	possibly damaging	Het
Fyb1	A	G	15: 6,673,232 (GRCm39)	D622G	possibly damaging	Het
Gm4787	T	A	12: 81,425,489 (GRCm39)	H223L	probably benign	Het
Grin3a	A	G	4: 49,771,179 (GRCm39)	V531A	probably damaging	Het
Gzmb	T	C	14: 56,498,622 (GRCm39)	D42G	probably benign	Het
Herc2	T	A	7: 55,784,803 (GRCm39)	Y1416N	possibly damaging	Het
Hps4	G	A	5: 112,525,905 (GRCm39)	S642N	possibly damaging	Het
Hsd17b6	A	G	10: 127,833,876 (GRCm39)	W2R	probably damaging	Het
Hspa4l	T	C	3: 40,715,349 (GRCm39)		probably null	Het
Igtp	A	G	11: 58,097,726 (GRCm39)	E299G		Het
Klhl28	T	C	12: 64,990,300 (GRCm39)	D547G	possibly damaging	Het
Leng8	G	C	7: 4,146,897 (GRCm39)	R473P	probably damaging	Het
Lin37	T	A	7: 30,257,055 (GRCm39)	N53Y	possibly damaging	Het
Lrp1	G	A	10: 127,430,979 (GRCm39)	Q357*	probably null	Het
Lrrfip2	A	T	9: 111,034,840 (GRCm39)	K176N	probably damaging	Het
Ltbp4	A	G	7: 27,028,186 (GRCm39)	S390P	unknown	Het
Map3k2	G	A	18: 32,353,625 (GRCm39)	R465H	possibly damaging	Het
Mthfd1	T	A	12: 76,350,517 (GRCm39)	V665E	probably damaging	Het
Myh3	A	T	11: 66,989,451 (GRCm39)	I1524F	probably benign	Het
Myo1g	A	G	11: 6,466,153 (GRCm39)	S339P	possibly damaging	Het
Nf1	A	G	11: 79,450,332 (GRCm39)	K506R	probably damaging	Het
Nudt21	A	G	8: 94,746,321 (GRCm39)	I225T	probably benign	Het
Or4c31	A	G	2: 88,291,942 (GRCm39)	K105R	probably benign	Het
Or56b1b	A	T	7: 108,164,780 (GRCm39)	L74Q	probably benign	Het
Or5g9	T	A	2: 85,552,527 (GRCm39)	Y259*	probably null	Het
Or8b3	A	G	9: 38,314,518 (GRCm39)	Y116C	probably damaging	Het
Or8c18	A	T	9: 38,203,969 (GRCm39)	I243F	probably damaging	Het
Pax2	C	T	19: 44,806,968 (GRCm39)	T299I	probably benign	Het
Pde4dip	C	T	3: 97,749,044 (GRCm39)	S99N	probably damaging	Het
Pdlim5	T	A	3: 142,010,174 (GRCm39)	H147L	possibly damaging	Het
Pfkfb3	T	A	2: 11,487,381 (GRCm39)	Y343F	probably damaging	Het
Phf10	A	G	17: 15,174,382 (GRCm39)	I232T	probably damaging	Het
Plcb3	C	T	19: 6,936,288 (GRCm39)	G745D	probably benign	Het
Pramel11	G	A	4: 143,624,381 (GRCm39)	L39F	probably damaging	Het
Recql	C	A	6: 142,322,515 (GRCm39)	C129F	possibly damaging	Het
Rrp1	C	T	10: 78,240,876 (GRCm39)	D288N	unknown	Het
Samd4	T	C	14: 47,254,066 (GRCm39)	Y177H	probably benign	Het
Sim1	T	A	10: 50,785,525 (GRCm39)	M198K	possibly damaging	Het
Six4	T	C	12: 73,155,964 (GRCm39)	I334M	probably damaging	Het
Slc27a6	T	C	18: 58,705,268 (GRCm39)	S217P	probably damaging	Het
Slc2a4	T	A	11: 69,836,218 (GRCm39)	K264N	probably benign	Het
Slc8b1	A	T	5: 120,670,939 (GRCm39)	I476F	probably benign	Het
Smcp	T	C	3: 92,491,838 (GRCm39)	D3G	unknown	Het
Tagap	A	T	17: 8,152,335 (GRCm39)	M507L	probably benign	Het
Thap11	A	G	8: 106,582,780 (GRCm39)	E263G	probably damaging	Het
Tmco1	C	T	1: 167,136,132 (GRCm39)		probably benign	Het
Top3a	T	G	11: 60,654,253 (GRCm39)		probably null	Het
Ube2q2l	T	A	6: 136,378,011 (GRCm39)	D273V	probably benign	Het
Vmn1r31	A	G	6: 58,449,073 (GRCm39)	V264A	unknown	Het
Vti1a	A	C	19: 55,316,865 (GRCm39)	M81L	probably benign	Het
Wscd1	T	C	11: 71,674,245 (GRCm39)		probably null	Het
Zfp595	T	C	13: 67,464,676 (GRCm39)	E532G	probably damaging	Het
Zranb1	T	A	7: 132,584,167 (GRCm39)	V539D	probably damaging	Het

Other mutations in Fstl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00093:Fstl4	APN	11	53,077,102 (GRCm39)	missense	probably benign	0.01
IGL00885:Fstl4	APN	11	53,039,809 (GRCm39)	missense	possibly damaging	0.90
IGL00915:Fstl4	APN	11	53,067,825 (GRCm39)	missense	probably benign
IGL00933:Fstl4	APN	11	53,077,588 (GRCm39)	missense	possibly damaging	0.80
IGL01022:Fstl4	APN	11	53,077,568 (GRCm39)	missense	probably benign	0.18
IGL01121:Fstl4	APN	11	52,705,464 (GRCm39)	missense	probably benign	0.00
IGL01656:Fstl4	APN	11	52,891,201 (GRCm39)	missense	probably damaging	1.00
IGL01805:Fstl4	APN	11	53,077,184 (GRCm39)	missense	probably damaging	1.00
IGL01997:Fstl4	APN	11	53,053,881 (GRCm39)	nonsense	probably null
IGL02386:Fstl4	APN	11	52,664,698 (GRCm39)	missense	probably benign	0.21
IGL02536:Fstl4	APN	11	53,024,851 (GRCm39)	splice site	probably benign
IGL02807:Fstl4	APN	11	53,077,501 (GRCm39)	missense	probably benign	0.03
IGL03037:Fstl4	APN	11	53,059,050 (GRCm39)	missense	possibly damaging	0.83
R0462:Fstl4	UTSW	11	53,077,229 (GRCm39)	missense	probably benign	0.09
R1190:Fstl4	UTSW	11	52,959,373 (GRCm39)	missense	probably benign
R1300:Fstl4	UTSW	11	52,959,454 (GRCm39)	missense	probably benign
R1626:Fstl4	UTSW	11	52,891,117 (GRCm39)	nonsense	probably null
R1695:Fstl4	UTSW	11	53,056,705 (GRCm39)	splice site	probably null
R1699:Fstl4	UTSW	11	53,059,005 (GRCm39)	missense	possibly damaging	0.81
R1727:Fstl4	UTSW	11	52,959,478 (GRCm39)	missense	probably damaging	1.00
R1752:Fstl4	UTSW	11	53,077,622 (GRCm39)	missense	probably benign	0.09
R1866:Fstl4	UTSW	11	53,077,225 (GRCm39)	missense	probably benign	0.00
R4689:Fstl4	UTSW	11	52,959,477 (GRCm39)	nonsense	probably null
R5126:Fstl4	UTSW	11	53,077,388 (GRCm39)	missense	possibly damaging	0.71
R5129:Fstl4	UTSW	11	53,077,266 (GRCm39)	missense	probably damaging	1.00
R5499:Fstl4	UTSW	11	52,959,374 (GRCm39)	missense	probably benign	0.01
R5578:Fstl4	UTSW	11	53,056,608 (GRCm39)	missense	probably damaging	1.00
R5715:Fstl4	UTSW	11	52,891,243 (GRCm39)	missense	possibly damaging	0.53
R6125:Fstl4	UTSW	11	53,077,130 (GRCm39)	missense	probably benign
R6177:Fstl4	UTSW	11	53,059,031 (GRCm39)	missense	probably benign	0.00
R6236:Fstl4	UTSW	11	53,077,162 (GRCm39)	missense	probably benign	0.00
R6311:Fstl4	UTSW	11	53,067,804 (GRCm39)	missense	probably damaging	1.00
R6611:Fstl4	UTSW	11	53,077,552 (GRCm39)	missense	probably benign	0.01
R6886:Fstl4	UTSW	11	53,077,277 (GRCm39)	missense	probably damaging	1.00
R7404:Fstl4	UTSW	11	53,024,898 (GRCm39)	missense	probably benign	0.03
R7423:Fstl4	UTSW	11	52,959,382 (GRCm39)	missense	possibly damaging	0.54
R7586:Fstl4	UTSW	11	52,963,256 (GRCm39)	missense	probably benign	0.00
R7756:Fstl4	UTSW	11	53,059,123 (GRCm39)	missense	possibly damaging	0.69
R7758:Fstl4	UTSW	11	53,059,123 (GRCm39)	missense	possibly damaging	0.69
R7775:Fstl4	UTSW	11	53,067,798 (GRCm39)	nonsense	probably null
R7953:Fstl4	UTSW	11	52,891,050 (GRCm39)	missense	probably benign	0.01
R8026:Fstl4	UTSW	11	52,959,496 (GRCm39)	missense	probably damaging	0.99
R8043:Fstl4	UTSW	11	52,891,050 (GRCm39)	missense	probably benign	0.01
R8375:Fstl4	UTSW	11	53,053,502 (GRCm39)	missense	possibly damaging	0.63
R8866:Fstl4	UTSW	11	52,963,233 (GRCm39)	missense	possibly damaging	0.54
R9182:Fstl4	UTSW	11	53,024,905 (GRCm39)	missense	probably damaging	0.98
R9297:Fstl4	UTSW	11	53,024,973 (GRCm39)	missense	possibly damaging	0.50
R9390:Fstl4	UTSW	11	52,891,102 (GRCm39)	missense	probably benign
R9396:Fstl4	UTSW	11	52,664,778 (GRCm39)	missense	probably benign
R9447:Fstl4	UTSW	11	53,077,166 (GRCm39)	missense	probably damaging	1.00
R9506:Fstl4	UTSW	11	53,024,950 (GRCm39)	missense	probably benign	0.18
R9518:Fstl4	UTSW	11	53,056,647 (GRCm39)	missense	possibly damaging	0.71
R9523:Fstl4	UTSW	11	53,075,466 (GRCm39)	missense	probably benign	0.04
R9586:Fstl4	UTSW	11	53,077,729 (GRCm39)	missense	probably benign	0.02
R9594:Fstl4	UTSW	11	52,664,694 (GRCm39)	missense	probably benign
X0013:Fstl4	UTSW	11	53,053,446 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATTCTTCATGTGAATGCATGGG -3'
(R):5'- ATTAGACAATCGGACACTGTAACAC -3'

Sequencing Primer
(F):5'- GCTGACCAATTAATTAGGCAGTGCTG -3'
(R):5'- CAGAAAAGCAGTCTGTATGAAGTGC -3'

Posted On

2021-12-30