Mutagenetix > Incidental Mutation

Incidental Mutation 'R9106:Slfn3'

692032

Institutional Source

Beutler Lab

Gene Symbol

Slfn3

Ensembl Gene

ENSMUSG00000018986

Gene Name

schlafen 3

Synonyms

MMRRC Submission

068970-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9106 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

83082156-83105980 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 83103458 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 110 (F110L)

Ref Sequence

ENSEMBL: ENSMUSP00000150425 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019130] [ENSMUST00000214041]

AlphaFold

A0A1L1STQ7

Predicted Effect

probably benign
Transcript: ENSMUST00000019130

SMART Domains

Protein: ENSMUSP00000019130
Gene: ENSMUSG00000018986

Domain	Start	End	E-Value	Type
Pfam:AlbA_2	165	303	5.5e-11	PFAM
low complexity region	394	412	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000214041
AA Change: F110L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

97% (63/65)

MGI Phenotype

PHENOTYPE: Mice homozygous for a targeted allele exhibit normal immune cell populations. [provided by MGI curators]

Allele List at MGI

All alleles(3) : Targeted(3)

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahctf1	C	A	1: 179,614,601 (GRCm39)	K394N	probably benign	Het
Banp	A	T	8: 122,705,372 (GRCm39)	T81S	possibly damaging	Het
Btn2a2	T	C	13: 23,662,465 (GRCm39)	E495G	probably benign	Het
Cfap69	T	C	5: 5,690,190 (GRCm39)	I158M	possibly damaging	Het
Cgnl1	C	A	9: 71,628,873 (GRCm39)		probably benign	Het
Clip1	T	A	5: 123,753,223 (GRCm39)	Q186L	probably damaging	Het
Clspn	G	A	4: 126,471,243 (GRCm39)		probably benign	Het
Cnst	A	G	1: 179,432,162 (GRCm39)	E224G	probably damaging	Het
Ctdsp1	T	C	1: 74,433,884 (GRCm39)	L155P	probably damaging	Het
D5Ertd579e	C	T	5: 36,773,682 (GRCm39)	A238T	probably benign	Het
Dnah6	T	C	6: 73,121,752 (GRCm39)	Y1410C	probably damaging	Het
Fam186a	AGCCGCTGCCGCTGCCGCTGCCGC	AGCCGCTGCCGCTGCCGC	15: 99,844,107 (GRCm39)		probably benign	Het
Farp2	T	C	1: 93,488,910 (GRCm39)		probably null	Het
Galnt7	T	G	8: 57,985,729 (GRCm39)	D547A	probably damaging	Het
Gm1527	C	A	3: 28,956,440 (GRCm39)	D135E	probably damaging	Het
Grm5	T	C	7: 87,723,747 (GRCm39)	I679T	probably damaging	Het
Hectd4	C	A	5: 121,467,619 (GRCm39)	R2523S	possibly damaging	Het
Hps4	G	A	5: 112,525,905 (GRCm39)	S642N	possibly damaging	Het
Htr1f	A	T	16: 64,746,637 (GRCm39)	S218R	probably damaging	Het
Lrit1	G	A	14: 36,776,891 (GRCm39)	A4T	unknown	Het
Ly6f	G	T	15: 75,141,706 (GRCm39)	D50Y	probably damaging	Het
Mafg	GGTTCTTCAGTGT	GGT	11: 120,520,415 (GRCm39)		probably null	Het
Map2	T	A	1: 66,454,522 (GRCm39)	Y1137*	probably null	Het
Map4k3	C	A	17: 81,035,257 (GRCm39)	R21L	possibly damaging	Het
Mapk10	A	T	5: 103,186,442 (GRCm39)	V90D	probably damaging	Het
Mapkapk3	T	A	9: 107,136,067 (GRCm39)	E219V	probably damaging	Het
Mideas	T	C	12: 84,199,327 (GRCm39)	Y1040C	probably damaging	Het
Mos	T	G	4: 3,871,457 (GRCm39)	I120L	probably benign	Het
Mrgprb4	A	G	7: 47,848,679 (GRCm39)	V83A	probably benign	Het
Mrnip	A	G	11: 50,065,768 (GRCm39)	Q19R	probably damaging	Het
Myrip	T	C	9: 120,261,544 (GRCm39)	S386P	probably benign	Het
Mzt2	A	G	16: 15,666,568 (GRCm39)	W141R	probably benign	Het
Ncam1	A	G	9: 49,428,856 (GRCm39)	Y710H	probably damaging	Het
Nlrp9c	G	T	7: 26,081,837 (GRCm39)	L630I	probably benign	Het
Odad2	A	T	18: 7,294,527 (GRCm39)	S29T	probably benign	Het
Oplah	C	A	15: 76,189,876 (GRCm39)	G150C	probably benign	Het
Or4c11b	A	G	2: 88,625,016 (GRCm39)	T97A	probably benign	Het
Or51q1	A	T	7: 103,628,581 (GRCm39)	M61L	probably damaging	Het
Or52a20	G	A	7: 103,366,737 (GRCm39)	C312Y	probably benign	Het
Or6k2	A	G	1: 173,986,369 (GRCm39)	Q10R	probably benign	Het
Patl1	A	G	19: 11,908,973 (GRCm39)	K460R	probably damaging	Het
Pdgfrb	A	T	18: 61,179,100 (GRCm39)		probably null	Het
Phldb2	T	C	16: 45,680,757 (GRCm39)	I17V	probably benign	Het
Ppp3r1	A	G	11: 17,144,789 (GRCm39)	D134G	probably damaging	Het
Ppp4r4	C	T	12: 103,570,315 (GRCm39)	T763I	probably benign	Het
Prmt9	A	G	8: 78,276,358 (GRCm39)	D61G	probably benign	Het
Ptk2	T	C	15: 73,131,457 (GRCm39)	M589V	possibly damaging	Het
Rabl6	A	G	2: 25,486,446 (GRCm39)	W153R	probably benign	Het
Rb1cc1	A	G	1: 6,319,109 (GRCm39)	I843V		Het
Resf1	T	C	6: 149,230,368 (GRCm39)	V1138A	possibly damaging	Het
Rnf19b	A	G	4: 128,977,940 (GRCm39)	E719G		Het
Sart3	T	C	5: 113,892,410 (GRCm39)	D363G	possibly damaging	Het
Sgo2a	C	A	1: 58,037,283 (GRCm39)	D9E	possibly damaging	Het
Slc27a5	A	G	7: 12,725,097 (GRCm39)	V450A	probably benign	Het
Slc8b1	T	A	5: 120,668,416 (GRCm39)	Y483N	probably damaging	Het
Slco1b2	A	G	6: 141,617,974 (GRCm39)	T475A	probably damaging	Het
Spata31e5	T	C	1: 28,815,975 (GRCm39)	I686V	probably benign	Het
Ssr2	T	A	3: 88,495,269 (GRCm39)	Y175N	probably damaging	Het
Tagap	A	T	17: 8,150,280 (GRCm39)	N222Y	probably damaging	Het
Tecta	C	T	9: 42,278,479 (GRCm39)	V1010M	probably benign	Het
Trim36	A	T	18: 46,300,664 (GRCm39)	I669N	possibly damaging	Het
Ubp1	A	G	9: 113,799,319 (GRCm39)	T425A	probably benign	Het
Usp47	T	C	7: 111,681,713 (GRCm39)	I508T	probably damaging	Het
Vit	G	A	17: 78,934,278 (GRCm39)	D627N	probably damaging	Het
Vmn2r59	A	T	7: 41,695,884 (GRCm39)	I176N	probably benign	Het

Other mutations in Slfn3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00843:Slfn3	APN	11	83,104,257 (GRCm39)	missense	probably damaging	1.00
IGL01405:Slfn3	APN	11	83,105,542 (GRCm39)	missense	possibly damaging	0.90
IGL01631:Slfn3	APN	11	83,104,361 (GRCm39)	missense	probably damaging	0.99
IGL01944:Slfn3	APN	11	83,103,974 (GRCm39)	missense	possibly damaging	0.59
IGL02354:Slfn3	APN	11	83,104,068 (GRCm39)	missense	possibly damaging	0.95
IGL02361:Slfn3	APN	11	83,104,068 (GRCm39)	missense	possibly damaging	0.95
IGL02512:Slfn3	APN	11	83,103,851 (GRCm39)	missense	possibly damaging	0.55
IGL02875:Slfn3	APN	11	83,104,253 (GRCm39)	missense	probably damaging	0.98
IGL02944:Slfn3	APN	11	83,103,837 (GRCm39)	missense	probably damaging	0.99
IGL03402:Slfn3	APN	11	83,104,257 (GRCm39)	missense	probably damaging	1.00
R0452:Slfn3	UTSW	11	83,103,954 (GRCm39)	missense	possibly damaging	0.87
R0506:Slfn3	UTSW	11	83,103,986 (GRCm39)	missense	probably damaging	0.99
R0560:Slfn3	UTSW	11	83,103,978 (GRCm39)	missense	probably damaging	0.99
R0788:Slfn3	UTSW	11	83,103,662 (GRCm39)	missense	possibly damaging	0.47
R1602:Slfn3	UTSW	11	83,103,541 (GRCm39)	missense	probably damaging	1.00
R1713:Slfn3	UTSW	11	83,104,140 (GRCm39)	missense	probably damaging	0.98
R1881:Slfn3	UTSW	11	83,104,202 (GRCm39)	missense	possibly damaging	0.80
R2264:Slfn3	UTSW	11	83,103,798 (GRCm39)	missense	probably benign	0.00
R2441:Slfn3	UTSW	11	83,103,509 (GRCm39)	missense	probably benign	0.00
R2921:Slfn3	UTSW	11	83,105,871 (GRCm39)	missense	probably benign	0.01
R4163:Slfn3	UTSW	11	83,103,596 (GRCm39)	missense	probably damaging	1.00
R5099:Slfn3	UTSW	11	83,105,764 (GRCm39)	missense	probably damaging	0.98
R5448:Slfn3	UTSW	11	83,105,431 (GRCm39)	missense	probably damaging	0.99
R6441:Slfn3	UTSW	11	83,105,740 (GRCm39)	missense	probably benign	0.00
R6527:Slfn3	UTSW	11	83,103,932 (GRCm39)	missense	probably benign	0.01
R6785:Slfn3	UTSW	11	83,105,427 (GRCm39)	missense	possibly damaging	0.73
R7128:Slfn3	UTSW	11	83,105,721 (GRCm39)	missense	probably benign	0.00
R7344:Slfn3	UTSW	11	83,103,648 (GRCm39)	missense	probably benign	0.28
R7528:Slfn3	UTSW	11	83,105,731 (GRCm39)	missense	probably benign	0.01
R7763:Slfn3	UTSW	11	83,105,614 (GRCm39)	missense	possibly damaging	0.95
R8155:Slfn3	UTSW	11	83,103,611 (GRCm39)	missense	probably damaging	1.00
R8178:Slfn3	UTSW	11	83,105,505 (GRCm39)	missense	probably benign	0.33
R8210:Slfn3	UTSW	11	83,105,332 (GRCm39)	missense	possibly damaging	0.48
R8347:Slfn3	UTSW	11	83,104,415 (GRCm39)	missense	possibly damaging	0.95
R8671:Slfn3	UTSW	11	83,103,825 (GRCm39)	missense	probably benign	0.00
R9093:Slfn3	UTSW	11	83,103,948 (GRCm39)	missense	probably damaging	0.99
R9293:Slfn3	UTSW	11	83,105,616 (GRCm39)	missense	possibly damaging	0.85
R9362:Slfn3	UTSW	11	83,103,807 (GRCm39)	missense	probably benign
R9521:Slfn3	UTSW	11	83,103,825 (GRCm39)	missense	probably benign
R9522:Slfn3	UTSW	11	83,103,825 (GRCm39)	missense	probably benign
R9644:Slfn3	UTSW	11	83,105,728 (GRCm39)	missense	probably damaging	1.00
Z1176:Slfn3	UTSW	11	83,104,235 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAGCTGAGATGGTTGTGCTC -3'
(R):5'- TAGTCATGAGGAAGGTGCATGATAC -3'

Sequencing Primer
(F):5'- CTCTTTGTGAGGTTAAGCAGAATTGC -3'
(R):5'- TGCATGATACCGGGTAAGGAATTTAC -3'

Posted On

2021-12-30